Isolation and the Full-length Genome Sequencing of Subgroup J Avian Leukosis Virus ZH-08 Isolate Associated with Hemangioma

A subgroup J avian leukosis virus (AVL-J), designated as ZH-08, was isolated from a breeder flock in Guangdong province with a novel hemangioma case. The identification results of ELISA test, PCR and immunofluoresecence assay (IFA) specific for ALV-J were all positive. Based on the public full-length proviral genome sequence of ALV-J prototype strain HPRS-103, three pairs of primers were synthesized. The full-length proviral genome sequence of ZH-08 isolate is 7597 bp, which has a little difference with that of published full-length genome sequences, but its organization corresponds with typical retroviral genome structure; and known oncogenes were not included in its genome. According to the gp85 sequence comparison of ZH-08 isolate with those of the other reference strains in China and abroad, the highest similarity (93.7%) was with the YZ9901 isolate. Phylogenetic analysis, based on the gp85 gene, showed that the ZH-08 isolated here had the closest linkage to the SD07LK1 isolate. This study provides the basis for the biological characterization and pathogenesis research of the ZH-08 isolate.

Assessment of Flow Drive by Use of Ion Bernstein Wave on Heliotron J and EAST Devices

The possibility of driving poloidal flows by use of ion Bernstein wave is assessed for Heliotron J and EAST devices by means of ray tracing analysis. Sheared poloidal flow is expected to suppress plasma turbulences due to the decorrelation of the waves. In Heliotron J and EAST plasma, the rays of Ion Bernstein Wave travel into the central region with oscillations along the magnetic lines of force and their power is absorbed by ions at the cyclotron resonance layers. The momentum input has been estimated by calculating the momentum change of rays and the poloidal flow has been estimated using neoclassical viscosities. The wave momentum changes its sign as it propagates inward, depositing sheared momentum to the plasma, and therefore causes sheared poloidal flows.

Isolation of Three Strains of Avian Leukosis Virus Subgroup J and gp85 Gene Sequence Analysis

[Objective] To isolate three strains of avian leukosis virus subgroup J（ALV -J） , and then amplify and sequence the gp85 gene. [ Method] Three strains of ALV- J were isolated from Hubei Province, which were identified by pathological anatomy, DF- 1 cell culture and RT- PCR. And then they were named HB1002, HB1003 and HB1009, respectively. [ Result] Test sequence analysis showed, the length of gp85 gene was 921 bp, consistent with expect result; the nucleotide homology between the three isolates was in 97.7% -99.7% ,the homology of amino acid was in 95.1% - 99%. The nucleotide homology between HPRS - 103 and the three isolates was in the 94.1% - 94.8% ; and the nucleotide homolo- gy between other ALV -J and the three isolates was in 87.6% -97.3%. The phylogenetic trees analysis showed that the homology of JS09GY6 vi- rus and the three isolates was nearest, in 95.2% -97.3%. [ Conclusion] In the test, the three strains of virus which were isolated were ALV- J.

Image J Analysis of Six Different Annealed Temperatures of 0.17% C of HSLA Steels

Annealing is a heat treatment procedure in which the mechanical properties of a material are being altered, and such alterations cause changes in its properties such as strength and hardness. It is usually carried out to improve ductility and toughness, to reduce hardness and to remove carbides. This study deals with the use of image analysis processing method for the measurement of structure of six annealed samples of 0.17% High Strength Low Alloy (HSLA) Steels (840°C - 990°C) with 30°C interval and 30 minutes soaking time. From the optical microscope images using Image J program, some parameters like calculation of area, pixel value statistics, distances and angles measurements, edge detection, such as the circularity, ferret angle, solidity and perimeter, average area and the percentage area were examined. The annealed sample of 960°C gave the highest grain count of 543 with a perimeter of 41.518;standard deviation 7.057 and a mean of 28.722 which shows that annealing greatly improved the fatigue life of the steel. From the mechanical analysis result, the annealed sample at 840°C has a highest hardness value of 129.4 BHN and gave the lowest impact value of 58.75 J. This study shows a significant decrease in the hardness value as the annealing temperature increased which signifies that annealing temperature enhanced the fatigue property and tensile strength (wear strength) of the steel in study.

基于PACAP-cAMP-PKA信号通路探讨J型针刀调节神经源性膀胱逼尿肌的效应机制

目的:探讨J型针刀通过PACAP-cAMP-PKA信号通路改善脊髓损伤后神经源性膀胱大鼠逼尿肌的作用机制。方法:36只SD雄性大鼠随机分为6组:空白组、假手术组、模型组、J型针刀治疗组、治疗+Bupivacaine抑制剂组、治疗+H-89抑制剂组,各6只。采用脊髓横断法制作神经源性膀胱模型。空白组为正常SD大鼠;假手术组为切开相关部位组织,无脊髓切断;其余4组予以手术脊髓切断造模。J型针刀治疗组造模后第19天予J型针刀针刺大鼠次髎穴,2 d 1次,共治疗7次。干预结束后各组行HE染色观察膀胱逼尿肌组织形态变化,Elisa检测膀胱逼尿肌组织中c AMP、PKA含量,免疫组化检测膀胱逼尿肌组织中PACAP38蛋白表达,Western blot检测膀胱逼尿肌组织中PKA、p-MLC蛋白表达。结果:HE染色结果显示,与空白组相比,模型组中膀胱逼尿肌组织严重坏死,可见大量炎症细胞浸润;与模型组相比,J型针刀治疗组、治疗+Bupivacaine抑制剂组及治疗+H-89抑制剂组均有不同程度坏死组织修复情况。免疫组化检测PACAP38蛋白表达在模型组中表达量最低,针刀治疗之后,PACAP38表达量均不同程度上调;与J型针刀治疗组相比,治疗+Bupivacaine抑制剂组及治疗+H-89抑制剂组PACAP38蛋白表达均不同程度下调(P<0.05),但高于模型组(P<0.05)。Elisa检测结果显示,cAMP、PKA表达量模型组显著下调(P<0.05),J型针刀治疗组与模型组比显著上调(P<0.05),治疗+Bupivacaine抑制剂组及治疗+H-89抑制剂组比无显著差异(P>0.05)。WB结果显示:与空白组相比,模型组PKA、p-MLC蛋白表达上调;针刀治疗组表达量下调,其中PKA蛋白表达有显著性(P<0.05),p-MLC蛋白表达无显著性(P>0.05)。与J型针刀治疗组比,PKA蛋白表达量在治疗+Bupivacaine抑制剂组及治疗+H-89抑制剂组中无显著差异(P>0.05),p-MLC蛋白表达量显著下降(P<0.05)。结论:J型针刀针刺次髎穴可改善脊髓损伤后神经源性膀胱大鼠膀胱功能,其机制与J型针刀上调膀胱逼尿肌中PACAP38、cAMP、PKA表达,激活PACAP-cAMP-PKA信号通路,从而促进逼尿肌舒张有关。

ATP5J通过TOMM20调节线粒体功能并促进人肝细胞癌细胞转移

目的:探讨ATP合成酶H+转运线粒体F0复合体亚基F6(ATP5J)通过调节肝癌细胞线粒体功能介导细胞骨架重塑影响肝癌细胞转移的作用及其机制。方法:培养人肝细胞癌Li-7细胞株,基因修饰ATP5J表达(过表达和敲减)。使用JC-1染色检测每组细胞线粒体膜电位情况;利用DCFH-DA荧光探针检测Li-7细胞的活性氧(ROS)含量;线粒体ATP荧光探针检测线粒体功能;微丝绿色荧光探针(Actin-Tracker Green-488)检测细胞骨架重塑情况;Transwell检测细胞侵袭能力;Western blot检测ATP5J和线粒体外膜转位酶20(TOMM20)的表达水平。结果:过表达ATP5J可上调线粒体膜电位水平和线粒体ATP荧光强度、诱导细胞骨架重塑、促进细胞侵袭和TOMM20蛋白表达,抑制ROS生成(P<0.01)。相反,敲减ATP5J显著降低线粒体膜电位和线粒体ATP荧光强度,显著降低细胞侵袭能力和TOMM20表达,促进ROS产生,阻断细胞骨架重塑(P<0.01)。结论:ATP5J可调节肝癌细胞线粒体能量转化,其通过TOMM20调节线粒体膜电位水平和线粒体ATP产生介导细胞骨架重塑影响肝癌细胞转移。

‘Partly'globalized networks and driving mechanism in resource-based state-owned enterprises:A case study of J Group

In the context of economic globalization,while multinational enterprises from developed countries occupy a high-end position in the global value chain,enterprises from developing countries are often marginalized in the world market.In China,resource-based state-owned enterprises(SOEs)are tasked with the mission of safeguarding resource security,and their internationalization development ideas and strategic deployment are significantly and fundamentally different from those of other non-state-owned enterprises and large multinational corporations.This study provides ideas for the globalization policies of enterprises in developing countries.We consider J Group in western China as a case and discuss its productive investment and global production network development from 2010 to 2019.We found that J Group was‘Partly'globalized,and there are multiple core nodes with the characteristics of centralized and decentralized coexistence in the production network;in addition,the overall layout centre shifted to Southeast Asia and China;however,its global production was restricted by the enterprise's investment security considerations,support and restrictions of the home country,political security risk of the host country,and sanctions from the West.These findings provide insights for future research:under the wave of anti-globalization and'internal circulation as the main body',resource SOEs should consider the potential risk of investment,especially keeping the middle and downstream industrial chain in China as much as possible.

Morphological disruption and visual tuning alterations in the primary visual cortex in glaucoma(DBA/2J)mice

Glaucoma is a leading cause of irreve rsible blindness wo rldwide,and previous studies have shown that,in addition to affecting the eyes,it also causes abnormalities in the brain.However,it is not yet clear how the primary visual cortex(V1)is altered in glaucoma.This study used DBA/2J mice as a model for spontaneous secondary glaucoma.The aim of the study was to compare the electrophysiological and histomorphological chara cteristics of neurons in the V1between 9-month-old DBA/2J mice and age-matched C57BL/6J mice.We conducted single-unit recordings in the V1 of light-anesthetized mice to measure the visually induced responses,including single-unit spiking and gamma band oscillations.The morphology of layerⅡ/Ⅲneurons was determined by neuronal nuclear antigen staining and Nissl staining of brain tissue sections.Eighty-seven neurons from eight DBA/2J mice and eighty-one neurons from eight C57BL/6J mice were examined.Compared with the C57BL/6J group,V1 neurons in the DBA/2J group exhibited weaker visual tuning and impaired spatial summation.Moreove r,fewer neuro ns were observed in the V1 of DBA/2J mice compared with C57BL/6J mice.These findings suggest that DBA/2J mice have fewer neurons in the VI compared with C57BL/6J mice,and that these neurons have impaired visual tuning.Our findings provide a better understanding of the pathological changes that occur in V1 neuron function and morphology in the DBA/2J mouse model.This study might offer some innovative perspectives regarding the treatment of glaucoma.

一种基于Davinci和Neo4j图数据库的微博水军快速识别方法研究

研究探讨了大语言模型和图数据库技术在微博水军识别领域的应用。提出了一种基于Davinci模型的微博水军快速识别方法,并利用提示词工程识别水军账号概率及线索,同时运用Neo4j图数据库实施社群分析,绘制热点事件水军传播网络,识别水军团伙。结果表明,该方法能较好地识别微博水军账号,且速度快,结合人工研判效果明显。探讨了大语言模型在水军识别中的应用前景,研究分析了水军识别过程中基于标签数据集的模型微调、提示词构建、用户社群分析等关键技术。为相关部门提供了一种利用大语言模型工具支持分析工作的新思路,对未来水军监测工作具有一定参考价值。

ARG2基因在ALV-J感染DF-1细胞中的作用研究

为了研究线粒体相关基因精氨酸酶2(arginase-2,ARG2)在J亚群禽白血病病毒(J subgroup leukosis virus,ALV-J)感染中的作用,试验采用qRT-PCR方法分别检测感染ALV-J后试验组鸡不同器官和DF-1细胞中ARG2基因的表达情况,分析ARG2基因和ALV-J感染之间是否有联系;设计ARG2基因的过表达载体(过表达ARG2组)和干扰片段(干扰ARG2组)转染DF-1细胞,并以pcDNA3.1或Si-NC为对照,采用qRT-PCR方法检测ARG2基因的过表达和干扰效率;采用qRT-PCR、Western-blot和间接免疫荧光试验检测过表达或干扰ARG2基因后感染ALV-J的DF-1细胞中gp85基因及Env蛋白表达情况,从而综合分析ARG2基因对ALV-J复制的影响。结果表明:与对照组相比,试验组鸡的脾脏、法氏囊中ARG2基因相对表达量极显著降低(P<0.01),而肾脏中ARG2基因相对表达量极显著升高(P<0.01)。同时在体外试验中,ARG2基因在感染后第6,12小时时相对表达量降低(P>0.05或P<0.05),而在第24,48,74,108小时相对表达量显著或极显著升高(P<0.05或P<0.01)。与对照组相比,过表达ARG2组ARG2基因相对表达量极显著升高(P<0.01),干扰ARG2组ARG2基因相对表达量降低(P>0.05或P<0.05),并选择干扰片段SI-ARG2-001进行后续试验。过表达ARG2基因后,与对照组相比,过表达ARG2组第12,24小时的gp85基因相对表达量显著或极显著升高(P<0.05或P<0.01),Env蛋白表达量显著上调(P<0.05),Env免疫荧光信号强度增强;干扰ARG2基因后,与对照组相比,干扰ARG2组第6,12,48小时的gp85基因相对表达量显著或极显著降低(P<0.05或P<0.01),Env蛋白表达量显著下调(P<0.05),Env免疫荧光信号强度减弱。说明ARG2基因可以促进ALV-J在DF-1细胞中的复制。

一株Clade1.3 ALV-J分离鉴定及其全基因组序列分析

为了解地方品种鸡禽白血病病毒(ALV)感染情况,本试验用从广西某养殖公司地方品种鸡采集的血浆样品接种DF-1细胞进行ALV的培养分离,然后用ALV-p27抗原检测试剂盒对其细胞培养上清进行ELISA检测,并进一步对细胞培养物进行病毒亚群的PCR鉴定和病毒分离株的全基因组序列测定与分析。结果显示:从鸡血浆样品中获得一株ALV,分离毒株经分子鉴定及测序分析确定其为J亚群(ALV-J),命名为GX22YL01;通过对毒株GX22YL01的全病毒基因组与参考毒株序列进行比对分析,发现其与课题组建立的ALV-J分类方法“Pilot tree”中的参考株GX14HG04相似性最高,且同处于Clade 1.3分支。

C57BL/6J小鼠与昆明小鼠NIAAA法酒精性肝病造模效果的比较

为了对比C57BL/6J小鼠和昆明小鼠酒精性肝病模型建造效果及建模完成后续肝脏生化指标变化情况,为酒精性肝病的治疗研究提供基础依据,试验选择雌性C57BL/6J小鼠和昆明小鼠各36只,随机分为对照组(液体饲料+麦芽糊精)、建模16 d组与建模19 d组(液体饲料+酒精,n=12)。利用NIAAA法建造酒精性肝病小鼠模型,并分别于建模第16 d、建模第19 d取其血清及肝组织进行生化指标检测及病理切片观察。结果显示,C57BL/6J小鼠建模16 d组和建模19 d组与昆明小鼠建模16 d组的小鼠血清ALT、AST含量,肝脏MDA、TG含量均分别显著高于其对照组小鼠(P<0.05);而血清ALB含量、肝脏SOD活力显著低于其对照组小鼠(P<0.05)。C57BL/6J建模16 d组和建模19 d组小鼠的肝脏GSH含量和肝脏系数显著高于其对照组小鼠(P<0.05),但昆明小鼠建模19 d组除了肝脏SOD活力显著低于其对照组小鼠(P<0.05),其他指标均与其对照组小鼠无显著差异。病理切片结果表明,与对照组相比,建模16 d组2种小鼠肝细胞排列紊乱,肝细胞索断裂,肝细胞出现炎性浸润和脂肪液滴。C57BL/6J小鼠建模19 d组肝组织仍呈现病变,而昆明小鼠建模19 d组肝细胞已逐渐恢复正常排列。与昆明小鼠相比,C57BL/6J小鼠对于酒精的肝脏氧化应激损伤更严重,酒精性肝病模型更稳定。

Study of Type-B QPOs Observed in Black Hole X-Ray Binary Swift J1728.9-3613

We report the detection of type-B quasi-periodic oscillation(QPO)of the black hole X-ray binary Swift J1728.9-3613 observed by NICER during the 2019 outburst.A type-B QPO was observed for the first two days and it disappeared as flux increased,but again appeared at∼7.70 Hz when flux was dramatically decreased.The source was found in the soft intermediate state during these observations.We further studied the energy dependence of the QPO.We found that QPO was observed only for a higher energy range implying that the origin of QPO is possibly due to the corona emitting higher energy photons by the inverse Compton process.The variation of spectral parameters can be explained with the disk truncation model.The fractional rms was found to be monotonically increased with energy.The phase lag spectrum followed the“U-shaped”curve.The rms and phase lag spectrum are modeled and explained with the single-component Comptonization model vkompthdk.

Detection of Increasingγ-Ray Emissions from 4FGL J1718.5+4237 with Fermi-LAT

We report a gradual brighteningγ-ray source,4FGL J1718.5+4237,in 0.1-500.0 GeV,which may be associated with a blazar NVSS J171822+423948 with a redshift of~2.7.We analyzed 15.25 yr ofγ-ray data recorded by the Large Area Telescope on board the Fermi Gamma-ray Space Telescope and detected significantγ-ray emissions in the direction of the blazar with a test statistic(TS)of~135.Based on timing analysis using a 1 yr time bin,we have observed a gradual brightening inγ-ray emissions from the target.In our analysis,we categorize them into two states:Quiet(TS~0)and Loud(TS~226)states,with the distinction occurring in 2016 August(MJD57602.69).From the Quiet state to the brightest period(the last data point),theγ-ray flux in 0.1-500.0 GeV increased by more than 12-fold from<0.2×10^(-8)photons cm^(-1)s^(-1)to 2.6×10^(-8)photons cm^(-1)s^(-1).Additionally,we studied the spectral properties in detail for the Loud state and the overall data.While no significant variation was detected,both exhibited a spectral indexΓof~2.6.The origin of the brighteningγ-ray emissions from the target is not yet clear.Future long-term multi-wavelength observations and studies will provide insight into the astrophysical mechanisms of the target.

Searching for the Highest Energy of Pulsation and Critical Luminosity of Swift J0243.6+6124 Observed by Insight-HXMT

Owing to the broad energy coverage of Insight-HXMT in the hard X-ray band,we detected the highest energy of pulsation exceeding 200 keV around the 2017–2018 outburst peak of the first Galactic pulsating ultraluminous X-ray source (PULX) Swift J0243.6+6124,which is the highest energy detected from PULXs to date.We also obtained the highest energy of pulsation of every exposure during the outburst in 2017–2018,and found the highest energy is roughly positively correlated with luminosity.Using our newly developed method,we identified the critical luminosity being 4×10^(38)erg s^(-1) when the main peaks of the low and high energy pulse profiles became aligned,which separates the fan-beam dominated and pencil-beam dominated accretion regimes.Above the critical luminosity,the phase of the main peak shifted gradually from 0.5 to 0.8 until the outburst peak in al energy bands is reached,which is in agreement with the phase shift found previously at low energies.Our result is consistent with what is derived from spectral analysis.

RND3对雨蛙素诱导的大鼠AR42J细胞损伤的影响

目的:观察干预RND3对雨蛙素诱导的大鼠胰腺腺泡细胞AR42J损伤的影响,并探究RND3在急性胰腺炎(AP)中的调控机制。方法:分别构建RND3沉默和过表达的雨蛙素诱导的AR42J细胞AP模型,共6组(NC对照组、雨蛙素处理组、si-con阴性对照组、pcDNA阴性对照组、si-RND3沉默组和pcDNA-RND3过表达组)。qRT-PCR和Western blot分别在雨蛙素给药后的0 h、4 h、8 h、12 h和24 h检测AR42J细胞中RND3 mRNA、蛋白表达水平;CCK-8法检测细胞的存活情况和LDH漏出量,流式细胞术检测AR42J细胞的凋亡率,qRT-PCR检测相关炎症因子(TNF-α、IL-6、IL-1β和IL-18)的mRNA表达水平,Western blot检测AR42J细胞中凋亡相关蛋白(GSDMD、NLRP3、Cleaved caspase-1和caspase-1)表达。结果:雨蛙素诱导的AR42J细胞能抑制RND3 mRNA和蛋白表达,且随着雨蛙素处理时间延长,其对RND3的抑制作用越显著;相比NC对照组,siRND3组、雨蛙素处理组大鼠AR42J细胞中的相关炎症因子TNF-α、IL-6、IL-1β和IL-18表达上调(P<0.05),同时,凋亡相关蛋白GSDMD、NLRP3、Cleaved caspase-1和caspase-1表达升高(P<0.05);相反,pcDNA-RND3过表达组细胞炎症和凋亡水平显著降低(P<0.05)。结论:RND3可减轻大鼠胰腺腺泡细胞AR42J的炎症反应和细胞凋亡,其作用机制与NLRP3/caspase-1/GSDMD通路的活性有关。

血管性痴呆患者血清AIM2和载脂蛋白J水平表达与临床病情程度评估及预后的关系研究

目的探究血管性痴呆(vascular dementia,VD)患者血清黑色素瘤缺乏因子2(absent in melanoma 2,AIM2)、载脂蛋白J(apolipoprotein J,Apo J)水平与病情程度及预后的关系。方法选取滨州市第二人民医院2020年10月~2022年9月接诊的血管性痴呆患者128例作为研究组,依据简易精神状态检查表(mini-mental stateexamination,MMSE)评分评估病情严重程度,将研究组分为轻度组(n=43)、中度组(n=54)和重度组(n=31);治疗90天后根据日常生活能力(activities of daily living,ADL)量表评分评估患者预后,分为Ⅰ级组(n=66)、Ⅱ级组(n=40)和Ⅲ级组(n=22);另选取同期正常健康体检者96例作为对照组。应用实时荧光定量PCR(qRT-PCR)、酶联免疫吸附测定(ELISA)法检测血清AIM2和Apo J表达水平,并进行各组间比较;采用Spearman法分析血清AIM2,Apo J表达水平与MMSE评分的相关性以及与预后的关系;采用Logistic回归分析血管性痴呆患者预后影响因素。结果与对照组比较,研究组血清AIM2 mRNA(3.11±0.57 vs 1.06±0.23)表达水平显著升高,血清Apo J表达水平显著降低(68.83±12.24 ng/L vs 81.07±13.15 ng/L),差异具有统计学意义(t=32.054,7.174,均P<0.001);重度组血清AIM2 mRNA表达水平显著高于轻度组和中度组(q=12.807,15.780),血清Apo J表达水平、MMSE评分均明显低于轻度组和中度组(q=26.021,4.301;12.191,20.802),且中度组血清AIM2 mRNA表达水平显著高于轻度组(q=14.688),血清ApoJ表达水平、MMSE评分均低于轻度组(q=20.338,37.537),差异具有统计学意义(均P<0.001);血管性痴呆患者Ⅲ级组血清AIM2 mRNA(5.27±0.60)表达水平显著高于Ⅱ级组(3.36±0.58)、Ⅰ级组(2.23±0.55),而血清ApoJ(51.22±12.21 ng/L)表达水平显著低于Ⅱ级组(64.15±12.23 ng/L)、Ⅰ级组(77.53±12.25ng/L);Ⅱ级组血清AIM2 mRNA表达水平显著高于Ⅰ级组,而血清ApoJ表达水平显著低于Ⅰ级组,差异具有统计学意义(q=5.630~30.740,均P<0.001);血管性痴呆患者血清AIM2水平与MMSE评分呈负相关性,与预后(ADL分级)呈正相关性(r=-0.535,0.432,均P<0.001);血管性痴呆患者血清ApoJ水平与MMSE评分呈正相关性,与预后(ADL分级)呈负相关性(r=0.467,-0.496,均P<0.001);血清AIM2 mRNA[OR(95%CI):2.746(1.481~5.091),Apo J[OR(95%CI):0.496(0.311~0.791),MMSE评分[OR(95%CI):0.568(0.347~0.931)为血管性痴呆患者预后的影响因素(均P<0.05)。结论血管性痴呆患者血清AIM2 mRNA表达水平升高,血清Apo J表达水平降低,与患者病情程度及预后密切相关,可有效评估患者预后。

Spin Evolution of the Magnetar SGR J1935+2154

Fast radio bursts(FRBs)are short pulses observed in radio frequencies usually originating from cosmological distances.The discovery of FRB 200428 and its X-ray counterpart from the Galactic magnetar SGR J1935+2154suggests that at least some FRBs can be generated by magnetars.However,the majority of X-ray bursts from magnetars are not associated with radio emission.The fact that only in rare cases can an FRB be generated raises the question regarding the special triggering mechanism of FRBs.Here we report long time spin evolution of SGR J1935+2154 until the end of 2022.According to v and v,the spin evolution of SGR J1935+2154 could be divided into two stages.The first stage evolves relatively steady evolution until 2020 April 27.After the burst activity in2020,the spin of SGR J1935+2154 shows strong variations,especially for v.After the burst activity in 2022October,a new spin-down glitch with△v/v=(-7.2±0.6)×10^(-6)is detected around MJD 59876,which is the second event in SGR J1935+2154.At the end,spin frequency and pulse profile do not show variations around the time of FRB 200428 and radio bursts 221014 and 221021,which supply strong clues to constrain the trigger mechanism of FRBs or radio bursts.

A Possible γ-Ray Pulsation from PSR J1740-5340B in the Globular Cluster NGC 6397

Recently, a new radio millisecond pulsar(MSP) J1740-5340B, hosted in the globular cluster(GC) NGC 6397,was reported with a 5.78 ms spin period in an eclipsing binary system with a 1.97 days orbital period. Based on a modified radio ephemeris updated by tool tempo2, we analyze the ~15 yr γ-ray data obtained from the Large Area Telescope on board the Fermi Gamma-ray Space Telescope and detect PSR J1740-5340B's γ-ray pulsation at a confidence level of ~4σ with a weighted H-test value of ~26. By performing a phase-resolved analysis, the γ-ray luminosity in on-pulse interval of PSR J1740-5340B is L_(γ)~ 3.8 × 10^(33) erg s^(-1) using NGC 6397's distance of 2.48 kpc. And γ-rays from the on-pulse part of PSR J1740-5340B contribute ~90% of the total observed γ-ray emissions from NGC 6397. No significant γ-ray pulsation of another MSP J1740-5340A in the GC is detected.Considering that the previous four cases of MSPs in GCs, more data in γ-ray, X-ray, and radio are encouraged to finally confirm the γ-ray emissions from MSP J1740-5340B, especially starving for a precise ephemeris.

CAST/EiJ小鼠外周血和脾中免疫细胞及亚群分析

目的探索CAST/EiJ小鼠对多种病原体易感的可能原因,分析CAST/EiJ小鼠外周血和脾内的免疫细胞表型,明确CAST/EiJ小鼠的免疫细胞构成。方法利用流式细胞术,通过细胞表面标记物CD3、CD4、CD8、CD11b、CD11c、CD19、CD27、CD49b和TCRβ检测CAST/EiJ小鼠和C57BL/6J小鼠外周血和脾内经典型树突状细胞(cDCs)、自然杀伤细胞(NK)、B淋巴细胞、T淋巴细胞及其亚群的细胞比例。结果CAST/EiJ小鼠与C57BL/6J小鼠的cDCs细胞比例无明显差异,但cDC1细胞亚群比例偏低;CAST/EiJ小鼠的NK细胞(主要是成熟NK细胞亚群)和T淋巴细胞(主要是CD8+T细胞)比例都显著低于C57BL/6J小鼠,而CAST/EiJ小鼠的B淋巴细胞比例高于C57BL/6J小鼠。结论CAST/EiJ小鼠中NK和T淋巴细胞比例显著低于C57BL/6J小鼠。