The quasi-alliance strategy is part of the national security strategy and lies in the grey area between alliance and neutrality.1 In recent years,major countries,including Japan,have positioned quasi-alliance as a fea...The quasi-alliance strategy is part of the national security strategy and lies in the grey area between alliance and neutrality.1 In recent years,major countries,including Japan,have positioned quasi-alliance as a feasible unwritten option in their respective national security strategies.2 Japan’s quasialliance strategy was already in its infancy when former Japanese Prime Minister Shinzo Abe first came to power in September 2006 but was put on hold after his resignation in September 2007.展开更多
Introduced by the late Prime Minister Shinzo Abe and inherited and developed by Fumio Kishida,Japan's“Indo–Pacific”strategy has gradually taken shape.This strategy can be deemed a broad vision,covering a wide r...Introduced by the late Prime Minister Shinzo Abe and inherited and developed by Fumio Kishida,Japan's“Indo–Pacific”strategy has gradually taken shape.This strategy can be deemed a broad vision,covering a wide range of topics and an extensive network of partners,with a strong trend of pan-securitization.It is a comprehensive inter national st rateg y based on Japan's alliance policy and China containment strategy,following a global,security-oriented approach.Driven by considerations such as maintaining its economic status,realizing its long-cherished dream of becoming a political powerhouse,and containing China,Japan has stepped up its“Indo–Pacific”strategy,which may influence global development,undermine regional maritime security,and impede China's reunification process.Meanwhile,Japan's“Indo–Pacific”strategy faces the triple challenge of a strategic overdraft,the unstable economic foundations,and the weak external support.These constraints may not suffice to reverse the direction of Japan's“Indo–Pacific”strategy in the short term but will limit its effectiveness.展开更多
Sophora flavescens , which was first recorded in Shen Nong s Herbal Classic , has the functions of clearing away heat, drying dampness, killing parasites and promoting urination. In its long-term application, traditio...Sophora flavescens , which was first recorded in Shen Nong s Herbal Classic , has the functions of clearing away heat, drying dampness, killing parasites and promoting urination. In its long-term application, traditional Chinese medicine has gradually deepened its understanding of the origin and efficacy of S. flavescens . In order to explore changes in the origin and efficacy of traditional Chinese herb S. flavescens in the history of China and Japan, in this paper, the origin and development of S. flavescens in China and Japan were compared from three aspects: the origin, medicinal efficacy, and processing methods of S. flavescens in China and Japan, hoping to provide clear understanding of the medicinal plant S. flavescens .展开更多
On December 4th,CAFIU Vice-President Liu Hongcai met Mr.Kamada Takeaki,President of the JCI Japan-China Friendship Association(JCFA),to exchange views on China-Japan people-topeople and youth exchanges.Vice-President ...On December 4th,CAFIU Vice-President Liu Hongcai met Mr.Kamada Takeaki,President of the JCI Japan-China Friendship Association(JCFA),to exchange views on China-Japan people-topeople and youth exchanges.Vice-President Liu Hongcai said that JCFA has long been committed to promoting China-Japan youth exchanges and has played a positive role in enhancing mutual understanding between the Chinese and Japanese youths.展开更多
On May 27,China-Japan-ROK Trilateral Summit Meeting was held in Seoul after a hiatus of more than four years.Li Qiang,Premier of the State Council of the People’s Republic of China,Yoon Suk Yeol,President of the ROK,...On May 27,China-Japan-ROK Trilateral Summit Meeting was held in Seoul after a hiatus of more than four years.Li Qiang,Premier of the State Council of the People’s Republic of China,Yoon Suk Yeol,President of the ROK,and Fumio Kishida,Prime Minister of Japan,attended and addressed the Summit.展开更多
In this study, we investigated the variations in warming between Japanese cities for 1960-1989, and 1990-2019 using principal component analysis (PCA) and k-means clustering. The precipitation and sunshine hours exhib...In this study, we investigated the variations in warming between Japanese cities for 1960-1989, and 1990-2019 using principal component analysis (PCA) and k-means clustering. The precipitation and sunshine hours exhibited opposite tendencies in the PCA results. It was found that 1960M and 1990M had a correlation (r = 0.51). The 1960M and 1990M are the mean temperature anomalies in Japanese cities for 1960-1989 and 1990-2019, respectively. There was a strong correlation between temperature and precipitation (r = 0.62). There was an inverse correlation between 1960M and sunshine hours (r = −0.25), but a correlation between 1990M and sunshine hours (r = 0.11). Sunshine hours had less effect on the 1960M but more impact on the 1990M. The k-means clustering for 1960M and 1990M can be classified into four types: high 1960M and high 1990M, which indicates that global warming is progressing rapidly (Sapporo, Tokyo, Kyoto, Osaka, Fukuoka, Nagasaki), low 1960M and low 1990M, global warming is progressing slowly (Nemuro, Ishinomaki, Yamagata, Niigata, Fushiki, Nagano, Karuizawa, Mito, Suwa, Iida, Hamada, Miyazaki, Naha), low 1960M and high 1990M, global warming has accelerated since 1990 (Utsunomiya, Kofu, Okayama, Hiroshima), and normal 1960M and normal 1990M, the rate of warming is normal among the 38 cities (Asahikawa, Aomori, Akita, Kanazawa, Maebashi, Matsumoto, Yokohama, Gifu, Nagoya, Hamamatsu, Kochi, Kagoshima). Higher annual temperatures were correlated with higher annual precipitation according to the k-means clustering of temperature and precipitation. Two of the four categories consisted of places with high annual temperatures and high precipitation (Fushiki, Kanazawa, Kochi, Miyazaki, Kagoshima, Naha, Ishigakijima), and places with low annual temperatures and low precipitation (Asahikawa, Nemuro, Sapporo, Karuizawa).展开更多
为了给深入研究猪繁殖与呼吸综合征病毒(porcine reproductive and respiratory syndromevirus,PRRSV)ORF6基因编码的M蛋白的生物学功能提供重要试验材料,本研究首先利用慢病毒包装系统构建了过表达PRRSVORF6基因的重组慢病毒质粒,将该...为了给深入研究猪繁殖与呼吸综合征病毒(porcine reproductive and respiratory syndromevirus,PRRSV)ORF6基因编码的M蛋白的生物学功能提供重要试验材料,本研究首先利用慢病毒包装系统构建了过表达PRRSVORF6基因的重组慢病毒质粒,将该质粒连同辅助质粒共同转染至HEK293T细胞获得重组慢病毒;之后将重组慢病毒感染MARC-145细胞,利用嘌呤霉素结合有限稀释法进行筛选,连续筛选3轮后建立了稳定表达PRRSVM蛋白的MARC-145ORF6细胞系;并使用CCK-8试验评估过表达PRRSVM蛋白对MARC-145细胞生长的影响。利用RT-PCR、蛋白免疫印迹(Westernblot)和间接免疫荧光(IFA)评估MARC-145ORF6细胞系的传代稳定性并鉴定M蛋白的亚细胞定位,进一步利用RT-qPCR评估过表达M蛋白对MARC-145细胞的干扰素及相关调节基因的影响;此外,还测定了PRRSV在MARC-145ORF6细胞系、MARC-145Flag细胞系和MARC-145细胞中的病毒滴度并绘制多步生长曲线以比较其差异。CCK-8试验结果表明,过表达PRRSVM蛋白对MARC-145细胞活力无显著影响;RT-qPCR、Westernblot和IFA等试验结果表明,MARC-145ORF6细胞系能够表达PRRSV的M蛋白且在传代过程中稳定。此外,稳定表达PRRSVM蛋白显著下调了细胞系的Ⅰ型干扰素及其相关调节基因;多步生长曲线表明,MARC-145ORF6细胞系促进PRRSV增殖,提高其病毒滴度。综上,本研究构建了可以稳定表达PRRSVM蛋白的MARC-145ORF6细胞系,发现其Ⅰ型干扰素水平显著下调且促进PRRSV复制。本研究构建的MARC-145ORF6细胞系将为M蛋白功能的深入研究提供重要生物材料。展开更多
文摘The quasi-alliance strategy is part of the national security strategy and lies in the grey area between alliance and neutrality.1 In recent years,major countries,including Japan,have positioned quasi-alliance as a feasible unwritten option in their respective national security strategies.2 Japan’s quasialliance strategy was already in its infancy when former Japanese Prime Minister Shinzo Abe first came to power in September 2006 but was put on hold after his resignation in September 2007.
文摘Introduced by the late Prime Minister Shinzo Abe and inherited and developed by Fumio Kishida,Japan's“Indo–Pacific”strategy has gradually taken shape.This strategy can be deemed a broad vision,covering a wide range of topics and an extensive network of partners,with a strong trend of pan-securitization.It is a comprehensive inter national st rateg y based on Japan's alliance policy and China containment strategy,following a global,security-oriented approach.Driven by considerations such as maintaining its economic status,realizing its long-cherished dream of becoming a political powerhouse,and containing China,Japan has stepped up its“Indo–Pacific”strategy,which may influence global development,undermine regional maritime security,and impede China's reunification process.Meanwhile,Japan's“Indo–Pacific”strategy faces the triple challenge of a strategic overdraft,the unstable economic foundations,and the weak external support.These constraints may not suffice to reverse the direction of Japan's“Indo–Pacific”strategy in the short term but will limit its effectiveness.
基金Supported by Anhui University of Chinese Medicine(2023LCTH18,2021LCTH04)Fuyang Health Commission(FY2023-007)。
文摘Sophora flavescens , which was first recorded in Shen Nong s Herbal Classic , has the functions of clearing away heat, drying dampness, killing parasites and promoting urination. In its long-term application, traditional Chinese medicine has gradually deepened its understanding of the origin and efficacy of S. flavescens . In order to explore changes in the origin and efficacy of traditional Chinese herb S. flavescens in the history of China and Japan, in this paper, the origin and development of S. flavescens in China and Japan were compared from three aspects: the origin, medicinal efficacy, and processing methods of S. flavescens in China and Japan, hoping to provide clear understanding of the medicinal plant S. flavescens .
文摘On December 4th,CAFIU Vice-President Liu Hongcai met Mr.Kamada Takeaki,President of the JCI Japan-China Friendship Association(JCFA),to exchange views on China-Japan people-topeople and youth exchanges.Vice-President Liu Hongcai said that JCFA has long been committed to promoting China-Japan youth exchanges and has played a positive role in enhancing mutual understanding between the Chinese and Japanese youths.
文摘On May 27,China-Japan-ROK Trilateral Summit Meeting was held in Seoul after a hiatus of more than four years.Li Qiang,Premier of the State Council of the People’s Republic of China,Yoon Suk Yeol,President of the ROK,and Fumio Kishida,Prime Minister of Japan,attended and addressed the Summit.
文摘In this study, we investigated the variations in warming between Japanese cities for 1960-1989, and 1990-2019 using principal component analysis (PCA) and k-means clustering. The precipitation and sunshine hours exhibited opposite tendencies in the PCA results. It was found that 1960M and 1990M had a correlation (r = 0.51). The 1960M and 1990M are the mean temperature anomalies in Japanese cities for 1960-1989 and 1990-2019, respectively. There was a strong correlation between temperature and precipitation (r = 0.62). There was an inverse correlation between 1960M and sunshine hours (r = −0.25), but a correlation between 1990M and sunshine hours (r = 0.11). Sunshine hours had less effect on the 1960M but more impact on the 1990M. The k-means clustering for 1960M and 1990M can be classified into four types: high 1960M and high 1990M, which indicates that global warming is progressing rapidly (Sapporo, Tokyo, Kyoto, Osaka, Fukuoka, Nagasaki), low 1960M and low 1990M, global warming is progressing slowly (Nemuro, Ishinomaki, Yamagata, Niigata, Fushiki, Nagano, Karuizawa, Mito, Suwa, Iida, Hamada, Miyazaki, Naha), low 1960M and high 1990M, global warming has accelerated since 1990 (Utsunomiya, Kofu, Okayama, Hiroshima), and normal 1960M and normal 1990M, the rate of warming is normal among the 38 cities (Asahikawa, Aomori, Akita, Kanazawa, Maebashi, Matsumoto, Yokohama, Gifu, Nagoya, Hamamatsu, Kochi, Kagoshima). Higher annual temperatures were correlated with higher annual precipitation according to the k-means clustering of temperature and precipitation. Two of the four categories consisted of places with high annual temperatures and high precipitation (Fushiki, Kanazawa, Kochi, Miyazaki, Kagoshima, Naha, Ishigakijima), and places with low annual temperatures and low precipitation (Asahikawa, Nemuro, Sapporo, Karuizawa).
文摘为了给深入研究猪繁殖与呼吸综合征病毒(porcine reproductive and respiratory syndromevirus,PRRSV)ORF6基因编码的M蛋白的生物学功能提供重要试验材料,本研究首先利用慢病毒包装系统构建了过表达PRRSVORF6基因的重组慢病毒质粒,将该质粒连同辅助质粒共同转染至HEK293T细胞获得重组慢病毒;之后将重组慢病毒感染MARC-145细胞,利用嘌呤霉素结合有限稀释法进行筛选,连续筛选3轮后建立了稳定表达PRRSVM蛋白的MARC-145ORF6细胞系;并使用CCK-8试验评估过表达PRRSVM蛋白对MARC-145细胞生长的影响。利用RT-PCR、蛋白免疫印迹(Westernblot)和间接免疫荧光(IFA)评估MARC-145ORF6细胞系的传代稳定性并鉴定M蛋白的亚细胞定位,进一步利用RT-qPCR评估过表达M蛋白对MARC-145细胞的干扰素及相关调节基因的影响;此外,还测定了PRRSV在MARC-145ORF6细胞系、MARC-145Flag细胞系和MARC-145细胞中的病毒滴度并绘制多步生长曲线以比较其差异。CCK-8试验结果表明,过表达PRRSVM蛋白对MARC-145细胞活力无显著影响;RT-qPCR、Westernblot和IFA等试验结果表明,MARC-145ORF6细胞系能够表达PRRSV的M蛋白且在传代过程中稳定。此外,稳定表达PRRSVM蛋白显著下调了细胞系的Ⅰ型干扰素及其相关调节基因;多步生长曲线表明,MARC-145ORF6细胞系促进PRRSV增殖,提高其病毒滴度。综上,本研究构建了可以稳定表达PRRSVM蛋白的MARC-145ORF6细胞系,发现其Ⅰ型干扰素水平显著下调且促进PRRSV复制。本研究构建的MARC-145ORF6细胞系将为M蛋白功能的深入研究提供重要生物材料。
