Sophora flavescens , which was first recorded in Shen Nong s Herbal Classic , has the functions of clearing away heat, drying dampness, killing parasites and promoting urination. In its long-term application, traditio...Sophora flavescens , which was first recorded in Shen Nong s Herbal Classic , has the functions of clearing away heat, drying dampness, killing parasites and promoting urination. In its long-term application, traditional Chinese medicine has gradually deepened its understanding of the origin and efficacy of S. flavescens . In order to explore changes in the origin and efficacy of traditional Chinese herb S. flavescens in the history of China and Japan, in this paper, the origin and development of S. flavescens in China and Japan were compared from three aspects: the origin, medicinal efficacy, and processing methods of S. flavescens in China and Japan, hoping to provide clear understanding of the medicinal plant S. flavescens .展开更多
Introduced by the late Prime Minister Shinzo Abe and inherited and developed by Fumio Kishida,Japan's“Indo–Pacific”strategy has gradually taken shape.This strategy can be deemed a broad vision,covering a wide r...Introduced by the late Prime Minister Shinzo Abe and inherited and developed by Fumio Kishida,Japan's“Indo–Pacific”strategy has gradually taken shape.This strategy can be deemed a broad vision,covering a wide range of topics and an extensive network of partners,with a strong trend of pan-securitization.It is a comprehensive inter national st rateg y based on Japan's alliance policy and China containment strategy,following a global,security-oriented approach.Driven by considerations such as maintaining its economic status,realizing its long-cherished dream of becoming a political powerhouse,and containing China,Japan has stepped up its“Indo–Pacific”strategy,which may influence global development,undermine regional maritime security,and impede China's reunification process.Meanwhile,Japan's“Indo–Pacific”strategy faces the triple challenge of a strategic overdraft,the unstable economic foundations,and the weak external support.These constraints may not suffice to reverse the direction of Japan's“Indo–Pacific”strategy in the short term but will limit its effectiveness.展开更多
On May 27,China-Japan-ROK Trilateral Summit Meeting was held in Seoul after a hiatus of more than four years.Li Qiang,Premier of the State Council of the People’s Republic of China,Yoon Suk Yeol,President of the ROK,...On May 27,China-Japan-ROK Trilateral Summit Meeting was held in Seoul after a hiatus of more than four years.Li Qiang,Premier of the State Council of the People’s Republic of China,Yoon Suk Yeol,President of the ROK,and Fumio Kishida,Prime Minister of Japan,attended and addressed the Summit.展开更多
为了给深入研究猪繁殖与呼吸综合征病毒(porcine reproductive and respiratory syndromevirus,PRRSV)ORF6基因编码的M蛋白的生物学功能提供重要试验材料,本研究首先利用慢病毒包装系统构建了过表达PRRSVORF6基因的重组慢病毒质粒,将该...为了给深入研究猪繁殖与呼吸综合征病毒(porcine reproductive and respiratory syndromevirus,PRRSV)ORF6基因编码的M蛋白的生物学功能提供重要试验材料,本研究首先利用慢病毒包装系统构建了过表达PRRSVORF6基因的重组慢病毒质粒,将该质粒连同辅助质粒共同转染至HEK293T细胞获得重组慢病毒;之后将重组慢病毒感染MARC-145细胞,利用嘌呤霉素结合有限稀释法进行筛选,连续筛选3轮后建立了稳定表达PRRSVM蛋白的MARC-145ORF6细胞系;并使用CCK-8试验评估过表达PRRSVM蛋白对MARC-145细胞生长的影响。利用RT-PCR、蛋白免疫印迹(Westernblot)和间接免疫荧光(IFA)评估MARC-145ORF6细胞系的传代稳定性并鉴定M蛋白的亚细胞定位,进一步利用RT-qPCR评估过表达M蛋白对MARC-145细胞的干扰素及相关调节基因的影响;此外,还测定了PRRSV在MARC-145ORF6细胞系、MARC-145Flag细胞系和MARC-145细胞中的病毒滴度并绘制多步生长曲线以比较其差异。CCK-8试验结果表明,过表达PRRSVM蛋白对MARC-145细胞活力无显著影响;RT-qPCR、Westernblot和IFA等试验结果表明,MARC-145ORF6细胞系能够表达PRRSV的M蛋白且在传代过程中稳定。此外,稳定表达PRRSVM蛋白显著下调了细胞系的Ⅰ型干扰素及其相关调节基因;多步生长曲线表明,MARC-145ORF6细胞系促进PRRSV增殖,提高其病毒滴度。综上,本研究构建了可以稳定表达PRRSVM蛋白的MARC-145ORF6细胞系,发现其Ⅰ型干扰素水平显著下调且促进PRRSV复制。本研究构建的MARC-145ORF6细胞系将为M蛋白功能的深入研究提供重要生物材料。展开更多
Biomanufacturing(BM)is a multidisciplinary area incorporating the characteristics of living organisms and engineering principles to create valuable products for various sectors,including medicine,energy,and the enviro...Biomanufacturing(BM)is a multidisciplinary area incorporating the characteristics of living organisms and engineering principles to create valuable products for various sectors,including medicine,energy,and the environment.BM has undergone a remarkable transformation in the last two decades,entering the era of BM4.0 and becoming a pivotal driver of the sustainable revolution.Notably,Japan has made significant advances in BM,contributing to its development through the creation of innovative materials,advanced processes,and interdisciplinary applications.However,because of certain development policies,this research has not been widely recognized on an international level.This paper provides a comprehensive summary of the research progress made by renowned Japanese laboratories and researchers in biomedical materials,bio-three-dimensional(3D)printing,and biomedical applications in the last five years.Their unique contributions are introduced and analyzed,illuminating the distinctive approaches and breakthroughs within each domain.Additionally,this review highlights the current challenges and prospects of BM.The viewpoints presented in this paper are intended to serve as a valuable reference for scholars studying BM in Japan.展开更多
Mental health difficulties can impact students’motivation,focus,and ability to communicate with others.Students attending medical universities are more likely to experience anxiety,depression,and other mood changes f...Mental health difficulties can impact students’motivation,focus,and ability to communicate with others.Students attending medical universities are more likely to experience anxiety,depression,and other mood changes for thefirst time.However,no study has examined their prevalence among Japanese rehabilitation students.This study investigated the prevalence of anxiety among Japanese rehabilitation students and aimed to identify its predictors.A cross-sectional study was conducted among 148first-year physical and occupational therapy students at a private medical university in Japan in June 2022.Data on sociodemographic and personal characteristics,such as gender,age,subject major,regular exercise,place of residence,and sleeping hours,were recorded using an original questionnaire.Instruments included the State-Trait Anxiety Inventory,COVID-19 questionnaire,and Beck Depression Inventory–Second Edition(BDI-II).The results indicated that 83(56%)and 77(52%)students exhibited state anxiety and trait anxiety symptoms,respectively.Thirty-four(23%)students experienced mild depressive symptoms.Associations were further determined between gender,subject major,handling of stress,loneliness,satisfaction with life,satisfaction with social connections,anxiety about the future,satisfaction with school life,fulfillment of friendships,and depression.Several variables demonstrated a statistically significant association:handling stress(p<0.05;95%CI 0.409–2.472),satisfaction with life(p<0.01;95%CI 0.500–0.897),anxiety about the future(p<0.01;95%CI 1.397–4.087),and depression(total score of BDI-II,p<0.01;95%CI 1.555–100.742).Our results emphasize that universities must establish a system for early identification and support of anxiety and depression in students.展开更多
Ranking the first in tea production and sales globally,China occupies a decisive position in the global tea market.However,China’s tea export still faces problems such as low average export price,few international br...Ranking the first in tea production and sales globally,China occupies a decisive position in the global tea market.However,China’s tea export still faces problems such as low average export price,few international brands,single export market,and weak ability of market entities to deal with technical barriers to trade.Therefore,the analysis and summary of promotion measures for tea export taken by India and Japan is of great significance to the formulation of China’s tea export promotion policies.展开更多
On August 2nd,Vice-President Liu Hongcai met Takami Kunio,Deputy Representative of the Green Earth Network,Japan.The two sides exchanged views on China-Japan relations and people-to-topeople exchanges.
A total of 7,788 tons of treated radioactive water had been discharged into the Pacific Ocean from Japan’s wrecked Fukushima Daiichi Nuclear Power Plant by September 11 in the first round of disposal as planned,said ...A total of 7,788 tons of treated radioactive water had been discharged into the Pacific Ocean from Japan’s wrecked Fukushima Daiichi Nuclear Power Plant by September 11 in the first round of disposal as planned,said the plant operator Tokyo Electric Power Company(TEPCO),according to Japanese daily newspaper Sankei Shimbun.The second round of discharge scheduled for early October will release some 7,800 tons of nuclear-contaminated water.展开更多
The natural farming in Japan mainly includes Mokich Okadas natural farming theory and Masanobu Fukuokas natural farming theory in terms of genre.Natural farming was proposed and implemented to deal with the anabatic s...The natural farming in Japan mainly includes Mokich Okadas natural farming theory and Masanobu Fukuokas natural farming theory in terms of genre.Natural farming was proposed and implemented to deal with the anabatic soil degradation and food safety problems.Among themꎬMokich Okadas natural farming theory advocates the restoration of the agricultural tradition of the past and combination with modern agricultural science and technologyꎻMasanobu Fukuokas natural farming theory advocates a complete return to natural and traditional farming and puts forward a set of green agricultural cultivation methods focusing on injurious insect control with beneficial insectꎬweed control with green herbaceous plantsꎬsoil reclamation with grassꎬnontoxic and cost￣reducing method based on the Japanese ancient agricultural books.Their natural farming theories have been widely popularized and applied in Japanꎬand its successful experience is worthy of study and reference for China.展开更多
The Sea of Japan is located in the southeast margin of Eurasia, in the triangle area of the western Pacific Ocean. Due to the interaction of the Pacific plate, Eurasian plate and Philippine plate, its tectonic environ...The Sea of Japan is located in the southeast margin of Eurasia, in the triangle area of the western Pacific Ocean. Due to the interaction of the Pacific plate, Eurasian plate and Philippine plate, its tectonic environment is complex, forming a typical trench-arc-basin system. At present, 148 oil and gas fields have been discovered in Japan, with an oil and gas resource of 255.78×10^(6) t, showing a good prospect for oil and gas exploration. Based on the previous research and the recently collected geological and geophysical data, the characteristics of tectonic-sedimentary evolution and geothermal field in the basins around the Sea of Japan are analyzed. The results show that the tectonic evolution of the basin is mainly controlled by plate subduction and back-arc oceanic crust expansion, and it mainly undergone four tectonic-sedimentary evolution stages: Subduction period, basin development period, subsidence period and compression deformation period. The overall heat flow value of Japan Sea is high, and it is distributed annularly along Yamato Ridge. The geothermal heat flow value is about 50–130 MW/m^(2), and the average heat flow is75.9±19.8 MW/m^(2), which has a typical “hot basin ”. The high heat flow background provides unique thermal evolution conditions for hydrocarbon generation, which leads to the high temperature and rapid evolution. The authors summarized as “early hydrocarbon generation, rapid maturity and shallow and narrow hydrocarbon generation window”. The type of oil and gas is mainly natural gas, and it mainly distributed in Neogene oil and gas reservoirs. The trap types are mainly structural traps, lithologic traps and composite traps. In addition, the pre-Neogene bedrock oil and gas reservoirs also show a good exploration prospect. The resource prospecting indicates that Niigata Basin, Ulleung Basin and kitakami Basin are the main target areas for future exploration and development.展开更多
基金Supported by Anhui University of Chinese Medicine(2023LCTH18,2021LCTH04)Fuyang Health Commission(FY2023-007)。
文摘Sophora flavescens , which was first recorded in Shen Nong s Herbal Classic , has the functions of clearing away heat, drying dampness, killing parasites and promoting urination. In its long-term application, traditional Chinese medicine has gradually deepened its understanding of the origin and efficacy of S. flavescens . In order to explore changes in the origin and efficacy of traditional Chinese herb S. flavescens in the history of China and Japan, in this paper, the origin and development of S. flavescens in China and Japan were compared from three aspects: the origin, medicinal efficacy, and processing methods of S. flavescens in China and Japan, hoping to provide clear understanding of the medicinal plant S. flavescens .
文摘Introduced by the late Prime Minister Shinzo Abe and inherited and developed by Fumio Kishida,Japan's“Indo–Pacific”strategy has gradually taken shape.This strategy can be deemed a broad vision,covering a wide range of topics and an extensive network of partners,with a strong trend of pan-securitization.It is a comprehensive inter national st rateg y based on Japan's alliance policy and China containment strategy,following a global,security-oriented approach.Driven by considerations such as maintaining its economic status,realizing its long-cherished dream of becoming a political powerhouse,and containing China,Japan has stepped up its“Indo–Pacific”strategy,which may influence global development,undermine regional maritime security,and impede China's reunification process.Meanwhile,Japan's“Indo–Pacific”strategy faces the triple challenge of a strategic overdraft,the unstable economic foundations,and the weak external support.These constraints may not suffice to reverse the direction of Japan's“Indo–Pacific”strategy in the short term but will limit its effectiveness.
文摘On May 27,China-Japan-ROK Trilateral Summit Meeting was held in Seoul after a hiatus of more than four years.Li Qiang,Premier of the State Council of the People’s Republic of China,Yoon Suk Yeol,President of the ROK,and Fumio Kishida,Prime Minister of Japan,attended and addressed the Summit.
文摘为了给深入研究猪繁殖与呼吸综合征病毒(porcine reproductive and respiratory syndromevirus,PRRSV)ORF6基因编码的M蛋白的生物学功能提供重要试验材料,本研究首先利用慢病毒包装系统构建了过表达PRRSVORF6基因的重组慢病毒质粒,将该质粒连同辅助质粒共同转染至HEK293T细胞获得重组慢病毒;之后将重组慢病毒感染MARC-145细胞,利用嘌呤霉素结合有限稀释法进行筛选,连续筛选3轮后建立了稳定表达PRRSVM蛋白的MARC-145ORF6细胞系;并使用CCK-8试验评估过表达PRRSVM蛋白对MARC-145细胞生长的影响。利用RT-PCR、蛋白免疫印迹(Westernblot)和间接免疫荧光(IFA)评估MARC-145ORF6细胞系的传代稳定性并鉴定M蛋白的亚细胞定位,进一步利用RT-qPCR评估过表达M蛋白对MARC-145细胞的干扰素及相关调节基因的影响;此外,还测定了PRRSV在MARC-145ORF6细胞系、MARC-145Flag细胞系和MARC-145细胞中的病毒滴度并绘制多步生长曲线以比较其差异。CCK-8试验结果表明,过表达PRRSVM蛋白对MARC-145细胞活力无显著影响;RT-qPCR、Westernblot和IFA等试验结果表明,MARC-145ORF6细胞系能够表达PRRSV的M蛋白且在传代过程中稳定。此外,稳定表达PRRSVM蛋白显著下调了细胞系的Ⅰ型干扰素及其相关调节基因;多步生长曲线表明,MARC-145ORF6细胞系促进PRRSV增殖,提高其病毒滴度。综上,本研究构建了可以稳定表达PRRSVM蛋白的MARC-145ORF6细胞系,发现其Ⅰ型干扰素水平显著下调且促进PRRSV复制。本研究构建的MARC-145ORF6细胞系将为M蛋白功能的深入研究提供重要生物材料。
基金This work was supported by the National Natural Science Foundation of China(No.52105072).
文摘Biomanufacturing(BM)is a multidisciplinary area incorporating the characteristics of living organisms and engineering principles to create valuable products for various sectors,including medicine,energy,and the environment.BM has undergone a remarkable transformation in the last two decades,entering the era of BM4.0 and becoming a pivotal driver of the sustainable revolution.Notably,Japan has made significant advances in BM,contributing to its development through the creation of innovative materials,advanced processes,and interdisciplinary applications.However,because of certain development policies,this research has not been widely recognized on an international level.This paper provides a comprehensive summary of the research progress made by renowned Japanese laboratories and researchers in biomedical materials,bio-three-dimensional(3D)printing,and biomedical applications in the last five years.Their unique contributions are introduced and analyzed,illuminating the distinctive approaches and breakthroughs within each domain.Additionally,this review highlights the current challenges and prospects of BM.The viewpoints presented in this paper are intended to serve as a valuable reference for scholars studying BM in Japan.
文摘Mental health difficulties can impact students’motivation,focus,and ability to communicate with others.Students attending medical universities are more likely to experience anxiety,depression,and other mood changes for thefirst time.However,no study has examined their prevalence among Japanese rehabilitation students.This study investigated the prevalence of anxiety among Japanese rehabilitation students and aimed to identify its predictors.A cross-sectional study was conducted among 148first-year physical and occupational therapy students at a private medical university in Japan in June 2022.Data on sociodemographic and personal characteristics,such as gender,age,subject major,regular exercise,place of residence,and sleeping hours,were recorded using an original questionnaire.Instruments included the State-Trait Anxiety Inventory,COVID-19 questionnaire,and Beck Depression Inventory–Second Edition(BDI-II).The results indicated that 83(56%)and 77(52%)students exhibited state anxiety and trait anxiety symptoms,respectively.Thirty-four(23%)students experienced mild depressive symptoms.Associations were further determined between gender,subject major,handling of stress,loneliness,satisfaction with life,satisfaction with social connections,anxiety about the future,satisfaction with school life,fulfillment of friendships,and depression.Several variables demonstrated a statistically significant association:handling stress(p<0.05;95%CI 0.409–2.472),satisfaction with life(p<0.01;95%CI 0.500–0.897),anxiety about the future(p<0.01;95%CI 1.397–4.087),and depression(total score of BDI-II,p<0.01;95%CI 1.555–100.742).Our results emphasize that universities must establish a system for early identification and support of anxiety and depression in students.
文摘Ranking the first in tea production and sales globally,China occupies a decisive position in the global tea market.However,China’s tea export still faces problems such as low average export price,few international brands,single export market,and weak ability of market entities to deal with technical barriers to trade.Therefore,the analysis and summary of promotion measures for tea export taken by India and Japan is of great significance to the formulation of China’s tea export promotion policies.
文摘On August 2nd,Vice-President Liu Hongcai met Takami Kunio,Deputy Representative of the Green Earth Network,Japan.The two sides exchanged views on China-Japan relations and people-to-topeople exchanges.
文摘A total of 7,788 tons of treated radioactive water had been discharged into the Pacific Ocean from Japan’s wrecked Fukushima Daiichi Nuclear Power Plant by September 11 in the first round of disposal as planned,said the plant operator Tokyo Electric Power Company(TEPCO),according to Japanese daily newspaper Sankei Shimbun.The second round of discharge scheduled for early October will release some 7,800 tons of nuclear-contaminated water.
基金Supported by the Youth Program of the Ministry of Education's Humanities and Social Sciences Fund(22YJCZH220)the Major Program of Philosophy and Social Sciences Research in Jiangsu Universities(2022SJZD149).
文摘The natural farming in Japan mainly includes Mokich Okadas natural farming theory and Masanobu Fukuokas natural farming theory in terms of genre.Natural farming was proposed and implemented to deal with the anabatic soil degradation and food safety problems.Among themꎬMokich Okadas natural farming theory advocates the restoration of the agricultural tradition of the past and combination with modern agricultural science and technologyꎻMasanobu Fukuokas natural farming theory advocates a complete return to natural and traditional farming and puts forward a set of green agricultural cultivation methods focusing on injurious insect control with beneficial insectꎬweed control with green herbaceous plantsꎬsoil reclamation with grassꎬnontoxic and cost￣reducing method based on the Japanese ancient agricultural books.Their natural farming theories have been widely popularized and applied in Japanꎬand its successful experience is worthy of study and reference for China.
基金jointly supported by the Major Project of National Laboratory for Marine Science and Technology (Qingdao) (2021QNLM020001-1, 2021QNLM020001-4)the Project of China Geology Survey (DD20221723, DD20230317)+3 种基金the National Natural Science Foundation of China (41776075, 42076220 and 42206234)the Natural Science Foundation of Shandong Province (ZR2020QD038)the Major Basic Research Projects of Shandong Province (ZR2021ZD09)funded by the Qingdao Institute of Marine Geology, China Geological Survey。
文摘The Sea of Japan is located in the southeast margin of Eurasia, in the triangle area of the western Pacific Ocean. Due to the interaction of the Pacific plate, Eurasian plate and Philippine plate, its tectonic environment is complex, forming a typical trench-arc-basin system. At present, 148 oil and gas fields have been discovered in Japan, with an oil and gas resource of 255.78×10^(6) t, showing a good prospect for oil and gas exploration. Based on the previous research and the recently collected geological and geophysical data, the characteristics of tectonic-sedimentary evolution and geothermal field in the basins around the Sea of Japan are analyzed. The results show that the tectonic evolution of the basin is mainly controlled by plate subduction and back-arc oceanic crust expansion, and it mainly undergone four tectonic-sedimentary evolution stages: Subduction period, basin development period, subsidence period and compression deformation period. The overall heat flow value of Japan Sea is high, and it is distributed annularly along Yamato Ridge. The geothermal heat flow value is about 50–130 MW/m^(2), and the average heat flow is75.9±19.8 MW/m^(2), which has a typical “hot basin ”. The high heat flow background provides unique thermal evolution conditions for hydrocarbon generation, which leads to the high temperature and rapid evolution. The authors summarized as “early hydrocarbon generation, rapid maturity and shallow and narrow hydrocarbon generation window”. The type of oil and gas is mainly natural gas, and it mainly distributed in Neogene oil and gas reservoirs. The trap types are mainly structural traps, lithologic traps and composite traps. In addition, the pre-Neogene bedrock oil and gas reservoirs also show a good exploration prospect. The resource prospecting indicates that Niigata Basin, Ulleung Basin and kitakami Basin are the main target areas for future exploration and development.
