Fufang E’jiao Jiang’s effect on immunity,hematopoiesis,and angiogenesis via a systematic“compound-effect-target”analysis

Fufang E’jiao Jiang(FEJ)as a healthy food consisting of medicine food homology materials approved by China’s Ministry of Health has been extensively applied to replenish qi and nourish blood,and it has a positive impact on women’s health.To find out the material basis and mechanism of FEJ,a systematic“compoundeffect-target”analysis including chemical composition resolution,zebrafish,network pharmacology,molecular docking,transcriptome,and bibliometric analysis was adopted.124 chemical components including ginsenosides,and phenylethanoid glycosides in FEJ were discovered,and effects of FEJ on promoting the generation of immune cells,erythropoiesis and angiogenesis in zebrafish were exhibited.Based on network pharmacology,molecular docking and in vivo activity assay,6 compounds including jionoside A1,isoacteoside,echinacoside,acteoside,lobetyolin,and rehmannioside D were identified as active components of FEJ.Transcriptome data showed that several pathways such as complement and coagulation cascades,ECM-receptor interaction,and PI3K-Akt signaling pathway were associated with proangiogenic effect of FEJ.19 common targets were obtained through combined analysis of network pharmacology and transcriptomics,and 5 targets of them were verified by PCR.The bibliometric analysis of these common targets revealed that FEJ was related to energy metabolism,pathway in cancer,etc.,which was consistent with the results of network pharmacology and transcriptome.The studies suggested that FEJ could replenish qi and nourish blood through multi-compound and multi-targets.

Acupuncture at the San Jiao meridian affects brain stem tissue G protein content in a rat migraine model

BACKGROUND： G protein is closely associated with vasomotion. Vasomotor dysfunction accompanies migraine attack. OBJECTIVE： To investigate the effects of the San Jiao meridian acupuncture on G protein content in a rat migraine model. DESIGN, TIME AND SETTING： The present randomized grouping, cellular and molecular biological level trial was performed at the Institute of Integrated Traditional Chinese and Western Medicine, Xiangya Hospital, Central South University ＆ Key Laboratory for Tumor Proteomics of Ministry of Health between October 2003 and June 2004. MATERIALS： Forty healthy, male, Sprague Dawtey rats were included in this study. The G6805-2A electro-acupuncture apparatus was a product of Shanghai Huayi Medical Instrument Factory, China. Nitroglycerin was produced by Guangzhou Mingxing Pharmaceutical Factory, China. Antibodies against inhibitory and stimulatory G proteins were purchased from Sigma Chemical Company, USA. METHODS： All 40 rats were randomly and evenly divided into 4 groups. In the blank control group, the rats remained untouched. Rats from the normal control group were subcutaneously administered 2 mL/kg physiological saline. In the model group, migraine was induced with a subcutaneous injection of 10 mg/kg nitroglycerin （5 g/L）, and the rats received no further treatment. In the acupuncture-treated group, 30 minutes after migraine induction, acupuncture was performed at the bilateral Waiguan （SJ 5） and Yifeng （SJ 17） points, with an acupuncture depth of 1 mm. Electric-stimulation parameters of 20 Hz for low frequency, 40 Hz for high frequency, and 0.5-1.0 mA for current intensity were set. Ten acupuncture sessions were applied, with 20-minute low-frequency and 20-minute high-frequency stimulation and 3 seconds of interval time. MAIN OUTCOME MEASURES： Inhibitory and stimulatory G protein contents were detected by Western blot analysis. RESULTS： At 4 hours after migraine induction, compared with the blank control and normal control groups stimulatory G protein concentration was significantly increased, while inhibitory G protein levels were significantly decreased in the model group （P 〈 0.01 ）. In the acupuncture-treated group, both stimulatory and inhibitory G protein concentrations were significantly increased following acupuncture treatment （P 〈 0.01）, but stimulatory G protein levels were less and the inhibitory G protein concentrations were greater compared to the model group （P 〈 0.01 ）. There was no significant difference in stimulatory and inhibitory G protein levels between the blank control and normal control groups （P 〉 0.05）. CONCLUSION： Dysfunctional G protein signal transductions in the rat brain stem may be responsible tor migraine attack. Acupuncture at the San Jiao meridian ameliorates migraines by mediating the G protein signal transduction pathway.

Jiao-tai-wan Up-regulates Hypothalamic and Peripheral Circadian Clock Gene Cryptochrome and Activates PI3K/AKT Signaling in Partially Sleep-deprived Rats

This study aims to explore the effect and mechanism of Jiao-tai-wan （JTW） on systemic and tissue-specific inflammation and insulin resistance in obesity-resistant （OR） rats with chronic partial sleep deprivation （PSD）. OR rats with PSD were orally given JTW and Estazolam for 4 weeks. The amount of food intake and metabolic parameters such as body weight increase rate, fasting plasma glucose （FPG）, fasting insulin （FINS）, homeostasis model assessment-insulin resistance （HOMA-IR） and plasma inflammatory markers were measured. The expression levels of circadian proteins cryptochrome 1 （Cryl） and cryptochrome 2 （Cry2） in hypothalamus, adipose and liver tissues were also determined. Meanwhile, the mRNA expression of inflammatory markers, activity of nuclear factor kappa B （NF-κB） p65 protein, as well as the expression levels of insulin signaling pathway proteins in hypothalamus, adipose and liver tissues were measured. Additionally, cyclic adenosine 3＇, 5＇-monophosphate （cAMP） and activity of vasodilator-stimulated phosphoprotein （VASP） in hypothalamus tissue were measured. JTW significantly decreased the body weight increase rate and food intake, ameliorated systemic inflammation and insulin resistance. JTW effectively ameliorated inflammation and increased PI3K/AKT signaling activation in hypothalamus, adipose and liver. Interestingly, all these changes were associated with the up-regulation of circadian gene Cryl and Cry2 protein expression. We also found that in hypothalamus tissue of PSD rats, down-regulation of Cryl and Cry2 activated cAMP/PKA signaling and then led to inflammation, while JTW inhibited this signaling. These results suggested that JTW has the beneficial effect on ameliorating inflammation and insulin resistance in partially sleep-deprived rats by up-regulating Cry expression.

Rong Shuan Jiao Nang in the treatment of acute mountain sickness and high altitude myocardial ischemic syndrome in Yushu

To evaluate the therapeutic effects of Rong Shuan Jiao Nang （RSJN） on treatment of acute mountain sickness （AMS） and high altitude myocardial ischemic syndrome in workers in Yushu, three groups were studied： group A （60 patients with AMS, given RSJN）, group B （15 patients with altitude myocardial ischemic syndrome, given RSJN）, and group C （control, without drugs）. All studied subjects were lowland workers who were first time entry to Yushu for work at an altitude of 4 250 m. During the course of treatment, a routing physical examina- tion was performed, AMS Lake Louise Scores were estimated, arterial oxygen saturation （SaO2）, electrocardiography and hemoglobin concentration were measured before and after using RSJN for 10 days. In group A, the effective rate was 68 %, symptomatic improvement in 54 cases （90 %） within 5 days. In group B, the effective rate was 93 %, episodes of angina pectoris stopped in 12 patients within 3 - 7 days, one lasted 8 days. After treatment, the level of SaO2 increased 15.5 %, 21.8 % and 5.6 % in group A, group B and group C, respectively. RSJN tak- en at the start of the arrival at Yushu can decrease AMS scores and facilitate cure. If taken after the illness has begun, RSJN may help lessen symptoms, especially effectively improved angina pectoris of the high altitude myocardial ischemic syndrome. Symptoms usually subside after 3 - 8 days. RSJN should be continually used lbr at least 7 days after ascent.

Structure and Distribution of the San Jiao and Cou Li-Recognized Interstitium in Human Tissues

Since the Yellow Emperor’s Inner Canon(c.200 bce),Traditional Chinese Medicine(TCM)has held that the san jiao system is the largest anatomical structure in the human body,and that it consists of a network comprising the large cavities in the body trunk and the small interstitial spaces between the tissues and cells throughout the body.More than 2000 years later,and according to recent scientific reports in America,this network of structures has been recognized by modern medicine.The two theories,TCM’s san jiao and its system of spaces(腠còu),and the recent scientific discovery of an interstitial network in the human body,are quite similar in structure,distribution and function.

The early Proterozoic metamorphic geodynamics of the Jiao-Liao Massif, east China

Considering the mineral evolution and tectonic deformational history of the early Proterozoic metamorphic sequences in the Jiao Liao Massif, very similar CCW PTt D paths were established in east Liaoning and east Shandong district within this massif. Their style displays the process of developing and closing of some rifted depression belts within the rather stable continental crust of the Jiao Liao Massif during early Proterozoic, and they may be treated as a common kind of metamorphic geodynamic model.

A Brief Discussion on the Concept of Location San Jiao

San Jiao is one of the most controversial concepts in traditional Chinese medicine.With the changes of history,the concept of San Jiao was developed into another concept,"Location San Jiao",on the basis of"Liu Fu San Jiao".However,the appearance of"Location San Jiao"makes the concept of San Jiao more complicated and controversial.This paper will explore from the origin of"Location San Jiao",analyze the origin of its argument,and discuss the problems in it,so as to preliminarily clarify the connotation of San Jiao conception.Through investigation,it is found that the"Location San Jiao"is actually the dissimilation of"Liu Fu San Jiao",and the three parts of"Liu Fu San Jiao"should be divided by functional features rather than anatomical structure.

基于“三焦生精”理论治疗成人原发免疫性血小板减少症

原发免疫性血小板减少症是一种免疫介导的以血小板减少为主要特征的疾病,归属于中医“紫癜病”范畴,临床多从火热亢盛、气虚失摄、脾不统血、肾虚火旺等方面论治。三焦理论始于《黄帝内经》,三焦具有通行原气,运化水液两大基本功能,该文首先对“三焦生精”的理论内涵进行阐述,其次基于“三焦生精”理论探讨原发免疫性血小板减少症的病机及治法,最后通过对一则验案进一步分析,以期为临床治疗原发免疫性血小板减少症拓宽思路。

Literature and Time:On Jiao Xun’s Concept of Literary Transformation

Jiao Xun’s concept of the succession of literary genres according to each generation’s strengths clearly establishes the interrelationship between historical time and literary genres.Jiao Xun’s idea of the transformation of literary styles differs from the prevailing notion at that time,which advocated prioritizing poetry and prose that conveyed aspirations and ideals while considering other literary genres such as ci(a form of lyric poetry),qu(drama),and novels as inferior.Instead,Jiao Xun advocated using the“nature”and“events”of objects as criteria and awakened a more traditional“poetic teaching tradition”characterized by metaphorical expression and emotional resonance,which aligned with the concept of literature conveying aspirations and ideals.This enabled the standard of“nature”to transcend the boundaries of literary genres,establishing new criteria for the hierarchy of genres and encapsulating Jiao Xun’s intention to summon emerging literary genres that were in harmony with the“present”.

Discussion on "Wei Qi Chu Shang Jiao"

Due to the different versions of Nei Jing have been published in the past,variant of records have shown the different explanation of the original source of Wei Qi,thus there are two popular options are still under debate,Shang Jiao and Xia Jiao.Our research has found that the core reason of above dispute is caused by the vague understanding of the meaning of"Chu".Based on the analysis of relevant text of Nei Jing and the character of Chu with the meaning of leaving,it is concluded that"Wei Qi Chu Shang Jiao".And it means Wei Qi has left Shang Jiao of Zang Fu system and following into Meridian system,according to Wei Qi's connotation of following and protecting.Shang Jiao is the original starting point of Wei Qi in circulation just as it is born.And Shang Jiao is the place where"the slippery and tough Qi of water and grain"has transformed its name into"Wei Qi",which is also the conjunction point that Wei Qi changing from Ti(ontology)into Yong(methodology).And it explains the theory of clinical application based on this view.

芪苈强心胶囊降低慢性心力衰竭急性失代偿患者发生利尿剂抵抗风险的回顾性研究

背景利尿剂抵抗与心力衰竭(HF)患者的病死率增加有关。芪苈强心胶囊是用于治疗HF的中药。目前缺乏其在改善利尿剂抵抗方面作用的临床证据。目的探讨芪苈强心胶囊能否降低慢性HF急性失代偿(ADCHF)患者发生利尿剂抵抗的风险并改善其预后。方法纳入2018年1月—2022年6月在天津大学胸科医院CICU病区住院治疗的HF患者374例为研究对象,根据是否发生利尿剂抵抗将患者分为利尿剂抵抗组(118例)和非利尿剂抵抗组(256例)。收集患者的一般资料和实验室检查结果等。对患者随访12个月,观察因心血管事件再住院或全因死亡情况。绘制各组患者的Kaplan-Meyer生存曲线,生存曲线比较采用Log-rank检验。采用多因素Logistic回归分析探究患者发生利尿剂抵抗风险的影响因素。采用多因素Cox回归分析探究ADCHF患者发生终点事件的影响因素。结果利尿剂抵抗组年龄、体质量、N末端前体脑利钠肽(NT-proBNP)、血尿素氮、血肌酐、血尿酸、国际标准化比值(INR)高于非利尿剂抵抗组,24 h液体摄入量、估算肾小球滤过率(eGFR)、淋巴细胞绝对值、芪苈强心胶囊使用率低于非利尿剂抵抗组。多因素Logistic回归分析结果显示,使用芪苈强心胶囊(OR=0.363,95%CI=0.186~0.708,P=0.003)、24 h液体摄入量升高(OR=0.286,95%CI=0.177~0.461,P<0.001)是ADCHF患者发生利尿剂抵抗风险的保护因素。体质量增加(OR=1.064,95%CI=1.040~1.088,P<0.001)、血尿酸增高(OR=1.002,95%CI=1.000~1.004,P=0.027)是患者发生利尿剂抵抗风险的危险因素。Log-rank检验结果显示,利尿剂抵抗组无终点事件平均生存时间短于非利尿剂抵抗组(χ^(2)=11.866,P=0.001);未使用芪苈强心胶囊患者无终点事件平均生存时间短于使用芪苈强心胶囊患者(χ^(2)=6.502,P=0.011)。多因素Cox回归分析结果显示,使用芪苈强心胶囊(HR=0.536,95%CI=0.308~0.933,P=0.027)和使用血管紧张素受体脑啡肽酶抑制剂/血管紧张素转化酶抑制剂/血管紧张素受体阻滞剂(HR=0.435,95%CI=0.229~0.826,P=0.011)是患者发生终点事件的保护因素,总胆红素升高(HR=1.019,95%CI=1.008~1.030,P=0.001)、总胆汁酸升高(HR=1.029,95%CI=1.002~1.058,P=0.036)是患者发生终点事件的危险因素。结论在常规抗HF治疗的基础上使用芪苈强心胶囊可以降低ADCHF患者发生利尿剂抵抗的风险,同时降低这类患者1年内因心血管事件再住院或全因死亡的风险。

针刺关元-三阴交对DOR患者卵巢储备功能及卵巢血流灌注的影响

目的探究针刺关元-三阴交对卵巢储备功能下降(DOR)患者卵巢储备功能及卵巢血流灌注的影响。方法选取2021年1月至2022年12月我院收治的DOR患者80例进行研究。将80例患者均分为观察组和对照组各40例。对照组:基础治疗。观察组:基础治疗+针刺关元-三阴交。观察卵巢储备功能、卵巢血流灌注及不良反应。结果两组治疗前后的AFC比较差异均不显著(P>0.05),治疗前,两组FSH、FSH/LH、AMH差异不显著,P>0.05,治疗后的FSH、FSH/LH均降低,AMH增加,P<0.05,且观察组FSH(9.32±2.15)U/L、FSH/LH(1.81±0.21)低于对照组(10.77±2.22)U/L、(1.98±0.23),AMH(1.75±0.50)ng/mL高于对照组(1.51±0.54)ng/mL,差异显著,P<0.05。两组治疗前后的卵巢直径比较差异均不显著(P>0.05),治疗前,两组DOR患者PI、RI差异不显著,P>0.05,治疗后,PI、RI均降低,观察组PI(0.80±0.11)、RI(0.53±0.10)低于对照组(0.86±0.14)、(0.58±0.12),差异显著,P<0.05。两组均无血肿、感染、疼痛、晕针、滞针等不良反应。结论针刺关元-三阴交能够显著改善DOR患者卵巢储备功能及卵巢血流灌注,且安全性高。

The Idea of Edification in Jiao Xun’s Conception of Drama

Jiao Xun’s drama edification concept emphasizes“morality”and“emotion”at the same time,which expresses his tendency for drama conception to move closer to classics.Jiao Xun thinks that drama creation values the audience’s acceptance,which also makes drama an effective testing ground for re-processing the relationship between“morality”and“emotion”in Confucianism;and Jiao Xun respects folk daily life in popular literary forms to promote moral enlightenment,which shows that drama research has become one of his practical methods of“changing customs”.

胶辽吉造山带碰撞后岩浆作用:来自辽东-吉南地区巨斑状和球粒状花岗岩的约束

胶辽吉造山带辽东-吉南地区卧龙泉、双岔、八河川等巨斑状花岗岩体形成于造山峰期之后,记录了造山带演化的重要信息,对于探讨整个胶辽吉造山带构造演化具有重要意义。通过对巨斑状花岗岩研究,识别出局部侵入其中的球粒状花岗岩,并对采集自造山带东部和西部典型岩体的3件巨斑状花岗岩和1件球粒状花岗岩开展了岩相学、锆石U-Pb年代学、Lu-Hf同位素及主微量元素研究。结果显示巨斑状花岗岩具有硅含量相对较低、全碱较高、A/CNK值变化较大(0.86~1.19)等特征。虽然双岔巨斑状花岗岩中见有石榴子石,但全岩地球化学特征表现出准铝质、锆饱和温度较高等A型花岗岩特征;宝甸地区花岗岩中也有石榴子石,显示了S型花岗岩地球化学特征。卧龙泉和双岔巨斑状花岗岩体侵位时代为1880~1895Ma,球粒状花岗岩形成于1870±13Ma,宝甸地区巨斑状含石榴花岗岩侵位于1845±13Ma左右;来自这些锆石的原位εHf(t)值介于-3.6~+4.2之间,相应的二阶段模式年龄为2474~2730Ma。本研究认为,辽吉造山带碰撞后花岗质岩石成因较为复杂:球粒状花岗岩反映了岩浆过冷度较大、快速侵位至浅表过程;宝甸地区巨斑状花岗岩具有S型花岗岩特征;具有A型花岗岩特征的巨斑状花岗岩则反映了深部物质和加厚地壳均有贡献。结合前期报道的同时代碰撞后I型和A型花岗岩以及变质岩的研究等,本文认为辽东半岛巨斑状和球粒状花岗岩的产出与胶辽吉造山带碰撞后强烈的下地壳作用有关,反映了造山带去根垮塌、岩石圈伸展过程,代表了碰撞后花岗质岩浆作用。

ChronologicalInferenceofPuSongling。SPoems＂TOBeIII，SicknessFoot，andNiannujiao

路编《蒲松龄年谱》谓蒲氏《抱病》等诗写于康熙十八年，盛编《蒲松龄年谱》谓《病足》等诗写于康熙二十五年，袁世硕先生谓蒲袁酬答词全在康熙二十四年。其实，蒲氏《抱病》、《病足》、《念奴娇·新秋月夜，病中感赋，呈袁宣四孝廉》等作品，均有可能写于康熙十八年，“抱病”和“病足”说的实际是一回事。因此，有关聊斋诗词的编年问题，应该重新考虑。

龟鹿二仙胶诱导破骨细胞凋亡的机制研究

目的 探讨不同浓度龟鹿二仙胶对破骨细胞凋亡影响及相关机制。方法 RAW 264.7细胞培养传代,用M-CSF+RANKL诱导小鼠单核细胞RAW 264.7细胞株分化成破骨细胞,获得破骨细胞;CCK8法、TUNEL染色、流式细胞法检测不同浓度的龟鹿二仙胶对破骨细胞凋亡的影响;Real-time PCR法、Western blot法检测破骨细胞凋亡相关分子Bcl-2、Bax、Cytochrome C的表达;经龟鹿二仙胶及PI3K/AKT通路抑制剂LY294002干预后,通过流式细胞法、Western blot法检测破骨细胞凋亡率及凋亡相关蛋白AKT的变化。结果 不同浓度的龟鹿二仙胶(GLEXJ高、中、低)均能抑制破骨细胞的增殖活性,提高破骨细胞的凋亡率,提高凋亡相关分子Bax/Bcl-2的比值以及Cytochrome C的表达,其中,以中浓度GLEXJ作用72 h后影响最显著;经中浓度龟鹿二仙胶,以及PI3K/AKT通路抑制剂LY294002的干预,明显抑制了PI3K/AKT通路相关蛋白AKT蛋白的磷酸化,破骨细胞总凋亡率显著上升。结论 龟鹿二仙胶通过抑制PI3K/AKT通路活化作用而促进破骨细胞凋亡。

高校图书馆特色资源阅读推广实证研究——以上海交通大学馆藏地方历史文献为例

特色资源阅读推广是图书馆业务中极具挑战性的内容,由上海交通大学馆藏地方历史文献的阅读推广实践显示:文献学解读与数字化建设相结合的方式能够实现文献内容的深度挖掘,扩大阅读推广的范围。中华传统教育文化、中医药文化、红色文化等都在阅读推广中具有实效。场馆展览、文化活动与新媒体运营的结合同样构建了阅读推广服务的新路径,为高校文化育人打开了新的空间。