The Chinese medicine compound, ]isuikang, can promote recovery of neurological function by inhibiting lipid peroxidation, scavenging oxygen free radicals, and effectively improving the local microenvironment after spi...The Chinese medicine compound, ]isuikang, can promote recovery of neurological function by inhibiting lipid peroxidation, scavenging oxygen free radicals, and effectively improving the local microenvironment after spinal cord injury. However, the mechanism remains unclear. Thus, we established a rat model of acute spinal cord injury using a modified version of Allen＇s method. Jisuikang （50, 25, and 12.5 g/kg/d） and prednis- olone were administered 30 minutes after anesthesia. Basso, Beattie, and Bresnahan locomotor scale scores and the oblique board test showed improved motor function recovery in the prednisone group and moderate-dose Jisuikang group compared with the other groups at 3-7 days post-injury. The rats in the moderate-dose Jisuikang group recovered best at 14 days post-injury. Hematoxylin-eosin staining and transmis- sion electron microscopy showed that the survival rate of neurons in treatment groups increased after 3-7 days of administration. Further, the structure of neurons and glial cells was more distinct, especially in prednisolone and moderate-dose Jisuikang groups. Western blot assay and immunohistochemistry showed that expression of brain-derived neurotrophic factor （BDNF） in injured segments was maintained at a high level after 7-14 days of treatment. In contrast, expression of nerve growth factor （NGF） was down-regulated at 7 days after spinal cord injury. Re- al-time fluorescence quantitative polymerase chain reaction showed that expression of BDNF and NGF mRNA was induced in injured segments by prednisolone and Jisuikang. At 3-7 days after injury, the effect of prednisolone was greater, while 14 days after injury, the effect of moder- ate-dose Jisuikang was greater. These results confirm that Jisuikang can upregulate BDNF and NGF expression for a prolonged period after spinal cord injury and promote repair of acute spinal cord injury, with its effect being similar to prednisolone.展开更多
Objective:To explore the effects of chondroitinase ABC (Ch ABC) combined with spinal cord on neurological recovery and TGF-β1, HIF-1 and Nog-oNgR signaling pathways after spinal cord injury in rats.Methods: Sixty rat...Objective:To explore the effects of chondroitinase ABC (Ch ABC) combined with spinal cord on neurological recovery and TGF-β1, HIF-1 and Nog-oNgR signaling pathways after spinal cord injury in rats.Methods: Sixty rats were randomly divided into 4 groups: control group, model group, Ch ABC group and Ch ABC+ spinal cord. A rat spinal cord injury model was established using the Allen's method. Spinal nerve function was assessed by Basso Beattie Bresnahan (score) and somatosensory evoked potential (sEP) assays. mRNA levels were detected using RT-qPCR. Western blot was used to detect protein levels.Results: After modeling, the BBB scores of the rats were significantly decreased. After the intervention, the BBB scores of the three groups were improved. The BBB scores of the Ch ABC+ spinal cord group were significantly higher than those of the Ch ABC group. After modeling, the SEP of the rats was significantly increased. After the intervention, the BBB scores of the three groups were decreased. The BBB scores of the Ch ABC+ spinal cord group were significantly lower than those of the Ch ABC group. The TGF-β1 in the Ch ABC group and the Ch ABC+ spinal group was significantly higher than that in the model group, and the HIF-1 was significantly lower than the model group. The level of TGF-β1 in Ch ABC+ spinal cord group was significantly higher than that in Ch ABC group and HIF-1 was significantly lower than that in Ch ABC group. After treatment, the expression levels of Nog-oNgR pathway in Ch ABC group and Ch ABC + spinal cord group were significantly lower than those in model group, and the expression levels of Nogo-A, NgR and LINGO-1 in Ch ABC+ spinal cord group were significant after intervention. Lower than Ch ABC group.Conclusion: ChABC combined with spinal cord has a stronger role in promoting the recovery of neurological function. The combination of spinal cord can further inhibit the level of HIF-1 and increase the level of TGF-β1, and improve the prognosis to promote spinal healing. And the mechanism of action of the combination may be related to its role in inhibiting the growth of neurons by the Nogo-NgR signaling pathway.展开更多
Objective： To explore the effect of Jisuikang （脊髓康, JSK） on kinetic dysfunction in patients after spinal injury. Methods： Eighty-four patients with spinal injury were assigned equally, according to a randomizin...Objective： To explore the effect of Jisuikang （脊髓康, JSK） on kinetic dysfunction in patients after spinal injury. Methods： Eighty-four patients with spinal injury were assigned equally, according to a randomizing digital table to the treated group and the control group. Conventional treatment was given to both groups, and JSK was additionally given to the treated group. Changes of various kinetic function concerning parameters including kinetic score, grades of spinal injury, effectiveness of the treatment and available recovery rate in patients allocated in the treated group and the control group were observed and compared in the way issued by Association of Spinal Injury of America （ASIA）. Results： Better effects were shown in the treated group than those in the control group in improving kinetic score （92.00 ± 9.95 scores vs 83.76 ± 24.12 scores）, ASIA overall improvement rate （69.05% vs 45.24%） and grades of effectiveness （P〈0.05）. However, the difference of available recovery rate between the two groups was insignificant （P〉0.05）. Cenclusien： JSK could prevent secondary alteration of spinal injury, promote the recovery and regeneration of nerve tissues, but could not restore the function of a necrotic spine.展开更多
基金supported by the National Natural Science Foundation of China,No.81573997the Natural Science Foundation for Colleges and Universities in Jiangsu Province of China,No.15KJD360001the Natural Science Foundation of Jiangsu Province of China,No.BK2011180
文摘The Chinese medicine compound, ]isuikang, can promote recovery of neurological function by inhibiting lipid peroxidation, scavenging oxygen free radicals, and effectively improving the local microenvironment after spinal cord injury. However, the mechanism remains unclear. Thus, we established a rat model of acute spinal cord injury using a modified version of Allen＇s method. Jisuikang （50, 25, and 12.5 g/kg/d） and prednis- olone were administered 30 minutes after anesthesia. Basso, Beattie, and Bresnahan locomotor scale scores and the oblique board test showed improved motor function recovery in the prednisone group and moderate-dose Jisuikang group compared with the other groups at 3-7 days post-injury. The rats in the moderate-dose Jisuikang group recovered best at 14 days post-injury. Hematoxylin-eosin staining and transmis- sion electron microscopy showed that the survival rate of neurons in treatment groups increased after 3-7 days of administration. Further, the structure of neurons and glial cells was more distinct, especially in prednisolone and moderate-dose Jisuikang groups. Western blot assay and immunohistochemistry showed that expression of brain-derived neurotrophic factor （BDNF） in injured segments was maintained at a high level after 7-14 days of treatment. In contrast, expression of nerve growth factor （NGF） was down-regulated at 7 days after spinal cord injury. Re- al-time fluorescence quantitative polymerase chain reaction showed that expression of BDNF and NGF mRNA was induced in injured segments by prednisolone and Jisuikang. At 3-7 days after injury, the effect of prednisolone was greater, while 14 days after injury, the effect of moder- ate-dose Jisuikang was greater. These results confirm that Jisuikang can upregulate BDNF and NGF expression for a prolonged period after spinal cord injury and promote repair of acute spinal cord injury, with its effect being similar to prednisolone.
文摘Objective:To explore the effects of chondroitinase ABC (Ch ABC) combined with spinal cord on neurological recovery and TGF-β1, HIF-1 and Nog-oNgR signaling pathways after spinal cord injury in rats.Methods: Sixty rats were randomly divided into 4 groups: control group, model group, Ch ABC group and Ch ABC+ spinal cord. A rat spinal cord injury model was established using the Allen's method. Spinal nerve function was assessed by Basso Beattie Bresnahan (score) and somatosensory evoked potential (sEP) assays. mRNA levels were detected using RT-qPCR. Western blot was used to detect protein levels.Results: After modeling, the BBB scores of the rats were significantly decreased. After the intervention, the BBB scores of the three groups were improved. The BBB scores of the Ch ABC+ spinal cord group were significantly higher than those of the Ch ABC group. After modeling, the SEP of the rats was significantly increased. After the intervention, the BBB scores of the three groups were decreased. The BBB scores of the Ch ABC+ spinal cord group were significantly lower than those of the Ch ABC group. The TGF-β1 in the Ch ABC group and the Ch ABC+ spinal group was significantly higher than that in the model group, and the HIF-1 was significantly lower than the model group. The level of TGF-β1 in Ch ABC+ spinal cord group was significantly higher than that in Ch ABC group and HIF-1 was significantly lower than that in Ch ABC group. After treatment, the expression levels of Nog-oNgR pathway in Ch ABC group and Ch ABC + spinal cord group were significantly lower than those in model group, and the expression levels of Nogo-A, NgR and LINGO-1 in Ch ABC+ spinal cord group were significant after intervention. Lower than Ch ABC group.Conclusion: ChABC combined with spinal cord has a stronger role in promoting the recovery of neurological function. The combination of spinal cord can further inhibit the level of HIF-1 and increase the level of TGF-β1, and improve the prognosis to promote spinal healing. And the mechanism of action of the combination may be related to its role in inhibiting the growth of neurons by the Nogo-NgR signaling pathway.
文摘Objective： To explore the effect of Jisuikang （脊髓康, JSK） on kinetic dysfunction in patients after spinal injury. Methods： Eighty-four patients with spinal injury were assigned equally, according to a randomizing digital table to the treated group and the control group. Conventional treatment was given to both groups, and JSK was additionally given to the treated group. Changes of various kinetic function concerning parameters including kinetic score, grades of spinal injury, effectiveness of the treatment and available recovery rate in patients allocated in the treated group and the control group were observed and compared in the way issued by Association of Spinal Injury of America （ASIA）. Results： Better effects were shown in the treated group than those in the control group in improving kinetic score （92.00 ± 9.95 scores vs 83.76 ± 24.12 scores）, ASIA overall improvement rate （69.05% vs 45.24%） and grades of effectiveness （P〈0.05）. However, the difference of available recovery rate between the two groups was insignificant （P〉0.05）. Cenclusien： JSK could prevent secondary alteration of spinal injury, promote the recovery and regeneration of nerve tissues, but could not restore the function of a necrotic spine.
