[Objectives] This study was conducted to investigate the effects of lamb age and in vitro culture system of oocytes on the results of juvenile in vitro embryo transfer( JIVET). [Methods]Ten Dorper × small-tailed ...[Objectives] This study was conducted to investigate the effects of lamb age and in vitro culture system of oocytes on the results of juvenile in vitro embryo transfer( JIVET). [Methods]Ten Dorper × small-tailed Han lambs aged 5 to 10 weeks were induced to superovulate via i. p. injection of pregnant mare's serum gonadotropin( PMSG). The oocytes were matured in basal maturation solution or modified maturation solution,which was prepared by adding 200 μmol/L cysteine to the basal maturation solution. Then,the oocytes were fertilized in fertilization medium I containing 2% estrus sheep serum( ESS) or fertilization medium II containing 3 mg/ml bull serum albumin( BSA). Finally,the number of oocytes,oocyte maturation rate and cleavage rate of the lambs of different ages were determined. [Results]The average number of oocytes recovered per lamb was( 111. 00 ± 16. 97),( 139. 50 ± 28. 99),( 108. 50 ± 17. 68) and( 42. 00 ± 11. 31) for5-,7-,8-and 10-week-old Dorper × small-tailed Han lambs,respectively. The number of oocytes obtained from 5-,7-and 8-week-old lambs was significantly higher than that from 10-week-old lambs( P < 0. 05),but there was no significant difference among 5-,7-and 8-week-old lambs( P > 0. 05). The maturation rate of oocytes cultured in modified maturation solution was 3. 64% higher than that in basal maturation solution. The cleavage rate of oocytes in fertilization medium I was very significantly higher than that in fertilization medium II( P < 0. 01). [Conclusions] The results of JIVET can be improved by harvesting oocytes from lambs aged 5-8 weeks,adding a certain amount of cysteine into oocyte maturation solution,and a certain amount of ESS into fertilization medium.展开更多
The present study was conducted to explore ways of JIVET research on Texel sheep,and to provide technical support for the improvement of the JIVET technology system for research on Texel sheep.Superovulation was condu...The present study was conducted to explore ways of JIVET research on Texel sheep,and to provide technical support for the improvement of the JIVET technology system for research on Texel sheep.Superovulation was conducted on 4-8-week old Texel sheep,and in-vivo ovum collection,in-vitro oocyte maturation and fertilization,and fertilized-embryo culture and transplantation were adopted to perform JIVET research on Texel sheep.The results showed that 296 available oocytes were obtained at an average of 74 oocytes per sheep;205 fertilized eggs were obtained at the 2-4 cell stage at an average of 51.25 eggs per sheep and a cleavage rate of 69.26%;and 66 of the fertilized eggs were transplanted to 11 receptor sheep.Six sheep were conceived at a conception rate of 54.55%,and 11 lambs were born.These results indicated that the present test could be used as a method for the study of JIVET technique on Texel sheep.展开更多
探讨特克赛尔羊JIVET（juvenile in vitro embryo technology）技术,为优种绵羊JIVET技术体系完善提供技术支撑。试验对4-8周龄特克赛尔羊超数排卵,采用活体采卵的方法及对卵母细胞的体外成熟—受精—受精卵培养—受精卵移植的方法进行...探讨特克赛尔羊JIVET（juvenile in vitro embryo technology）技术,为优种绵羊JIVET技术体系完善提供技术支撑。试验对4-8周龄特克赛尔羊超数排卵,采用活体采卵的方法及对卵母细胞的体外成熟—受精—受精卵培养—受精卵移植的方法进行特克赛尔羊JIVET技术研究。结果显示：4只4-8周龄特克赛尔羊获得可用卵母细胞296枚,平均74枚/只;获得2-4细胞期受精卵205枚,卵裂率为69.26%,平均获2-4细胞期受精卵51.25枚/只;将66枚2-4细胞期受精卵移植给11只受体羊,6只羊受孕,生产11只羔羊,受胎率为54.55%。研究表明,本试验方法可作为特克赛尔羊JIVET技术研究的方法。展开更多
利用幼畜胚胎体外生产与胚胎移植(Juvenile in vitro embryo transfer,JIVET)技术能够获得大量卵母细胞,缩短世代间隔,加快育种进程。但幼畜卵母细胞形态及代谢与成年动物存在差异,这导致幼畜胚胎发育能力低。目前,对JIVET技术的研究主...利用幼畜胚胎体外生产与胚胎移植(Juvenile in vitro embryo transfer,JIVET)技术能够获得大量卵母细胞,缩短世代间隔,加快育种进程。但幼畜卵母细胞形态及代谢与成年动物存在差异,这导致幼畜胚胎发育能力低。目前,对JIVET技术的研究主要是通过改进激素处理方案及体外培养条件来增加卵母细胞数量并增强卵母细胞发育能力。从供体羔羊的选择、激素处理和卵母细胞的发育等方面对该技术进行了分析。展开更多
在羔羊时期,对母羔进行超数排卵、体外胚胎培养及移植等一系列的技术操作,称之为羔羊的超数排卵及体外胚胎生产技术(juvenile in vitro embryo technology,JIVET)。利用JIVET能够快速繁殖优秀种畜、缩短育种世代间隔、保护遗传资源,在...在羔羊时期,对母羔进行超数排卵、体外胚胎培养及移植等一系列的技术操作,称之为羔羊的超数排卵及体外胚胎生产技术(juvenile in vitro embryo technology,JIVET)。利用JIVET能够快速繁殖优秀种畜、缩短育种世代间隔、保护遗传资源,在遗传育种方面有巨大的优势,应用前景广阔。文章对羔羊JIVET的原理进行了综述,并分析了影响该技术应用效率的主要因素,以期为改进和发展羊JIVET提供参考。展开更多
本研究探讨两种常用的绵羊卵子体外成熟液对绵羔羊卵子体外成熟、受精和胚胎发育能力的影响,以期为改善绵羊JIVET技术体系提供借鉴。在绵羔羊卵子体外成熟方面,体外培养18 h后II液组卵子不但存活率显著高于I液组(0.82±0.02 vs 0.72...本研究探讨两种常用的绵羊卵子体外成熟液对绵羔羊卵子体外成熟、受精和胚胎发育能力的影响,以期为改善绵羊JIVET技术体系提供借鉴。在绵羔羊卵子体外成熟方面,体外培养18 h后II液组卵子不但存活率显著高于I液组(0.82±0.02 vs 0.72±0.03,P<0.05),而且卵子成熟率也显著高于I液组(0.63±0.02 vs 0.57±0.04,P<0.05);受精后胚胎发育方面,I液和II液组的卵裂率差异不显著(0.79±0.02 vs 0.81±0.02,P>0.05);然而II液组桑葚胚率显著高于I液组(0.71±0.02 vs 0.65±0.03,P<0.05)。总之,II液对绵羔羊卵子体外成熟、受精及发育的支持能力较好。此外,本研究进一步指出,II液中的半胱酰胺和丙酮酸钠可能是使其具有较强支持绵羔羊卵子成熟、发育能力的主要原因。展开更多
基金Supported by Special Fund for National Hair Sheep Industrial Technology System(CARS-39-24)Science and Technology Development Program of Shanxi Province(20120311024-1)+2 种基金Science and Technology Innovation Team Project of Shanxi Province(201705D131028-20)Financial Support of Agriculture of Shanxi Province(NYGX2015-03)Talent Project for Science and Technology Development in Outlaying Poor Areas,Frontier Ethnic Minority Areas and Old Revolutionary Base Areas of Shanxi Province,China(2017Sy128)
文摘[Objectives] This study was conducted to investigate the effects of lamb age and in vitro culture system of oocytes on the results of juvenile in vitro embryo transfer( JIVET). [Methods]Ten Dorper × small-tailed Han lambs aged 5 to 10 weeks were induced to superovulate via i. p. injection of pregnant mare's serum gonadotropin( PMSG). The oocytes were matured in basal maturation solution or modified maturation solution,which was prepared by adding 200 μmol/L cysteine to the basal maturation solution. Then,the oocytes were fertilized in fertilization medium I containing 2% estrus sheep serum( ESS) or fertilization medium II containing 3 mg/ml bull serum albumin( BSA). Finally,the number of oocytes,oocyte maturation rate and cleavage rate of the lambs of different ages were determined. [Results]The average number of oocytes recovered per lamb was( 111. 00 ± 16. 97),( 139. 50 ± 28. 99),( 108. 50 ± 17. 68) and( 42. 00 ± 11. 31) for5-,7-,8-and 10-week-old Dorper × small-tailed Han lambs,respectively. The number of oocytes obtained from 5-,7-and 8-week-old lambs was significantly higher than that from 10-week-old lambs( P < 0. 05),but there was no significant difference among 5-,7-and 8-week-old lambs( P > 0. 05). The maturation rate of oocytes cultured in modified maturation solution was 3. 64% higher than that in basal maturation solution. The cleavage rate of oocytes in fertilization medium I was very significantly higher than that in fertilization medium II( P < 0. 01). [Conclusions] The results of JIVET can be improved by harvesting oocytes from lambs aged 5-8 weeks,adding a certain amount of cysteine into oocyte maturation solution,and a certain amount of ESS into fertilization medium.
基金the Science and Technology Project of Shanxi Province(20120311024-1)Special Fund for the Construction of National Wool Sheep Industrial Technology Research System(CARS-39-24)+1 种基金Shanxi Provincial Science and Technology Innovation Team Fund(201705D131028-20)Shanxi Financial Fund for Agriculture(NYGX2015-03)。
文摘The present study was conducted to explore ways of JIVET research on Texel sheep,and to provide technical support for the improvement of the JIVET technology system for research on Texel sheep.Superovulation was conducted on 4-8-week old Texel sheep,and in-vivo ovum collection,in-vitro oocyte maturation and fertilization,and fertilized-embryo culture and transplantation were adopted to perform JIVET research on Texel sheep.The results showed that 296 available oocytes were obtained at an average of 74 oocytes per sheep;205 fertilized eggs were obtained at the 2-4 cell stage at an average of 51.25 eggs per sheep and a cleavage rate of 69.26%;and 66 of the fertilized eggs were transplanted to 11 receptor sheep.Six sheep were conceived at a conception rate of 54.55%,and 11 lambs were born.These results indicated that the present test could be used as a method for the study of JIVET technique on Texel sheep.
文摘探讨特克赛尔羊JIVET（juvenile in vitro embryo technology）技术,为优种绵羊JIVET技术体系完善提供技术支撑。试验对4-8周龄特克赛尔羊超数排卵,采用活体采卵的方法及对卵母细胞的体外成熟—受精—受精卵培养—受精卵移植的方法进行特克赛尔羊JIVET技术研究。结果显示：4只4-8周龄特克赛尔羊获得可用卵母细胞296枚,平均74枚/只;获得2-4细胞期受精卵205枚,卵裂率为69.26%,平均获2-4细胞期受精卵51.25枚/只;将66枚2-4细胞期受精卵移植给11只受体羊,6只羊受孕,生产11只羔羊,受胎率为54.55%。研究表明,本试验方法可作为特克赛尔羊JIVET技术研究的方法。
文摘利用幼畜胚胎体外生产与胚胎移植(Juvenile in vitro embryo transfer,JIVET)技术能够获得大量卵母细胞,缩短世代间隔,加快育种进程。但幼畜卵母细胞形态及代谢与成年动物存在差异,这导致幼畜胚胎发育能力低。目前,对JIVET技术的研究主要是通过改进激素处理方案及体外培养条件来增加卵母细胞数量并增强卵母细胞发育能力。从供体羔羊的选择、激素处理和卵母细胞的发育等方面对该技术进行了分析。
文摘在羔羊时期,对母羔进行超数排卵、体外胚胎培养及移植等一系列的技术操作,称之为羔羊的超数排卵及体外胚胎生产技术(juvenile in vitro embryo technology,JIVET)。利用JIVET能够快速繁殖优秀种畜、缩短育种世代间隔、保护遗传资源,在遗传育种方面有巨大的优势,应用前景广阔。文章对羔羊JIVET的原理进行了综述,并分析了影响该技术应用效率的主要因素,以期为改进和发展羊JIVET提供参考。
文摘本研究探讨两种常用的绵羊卵子体外成熟液对绵羔羊卵子体外成熟、受精和胚胎发育能力的影响,以期为改善绵羊JIVET技术体系提供借鉴。在绵羔羊卵子体外成熟方面,体外培养18 h后II液组卵子不但存活率显著高于I液组(0.82±0.02 vs 0.72±0.03,P<0.05),而且卵子成熟率也显著高于I液组(0.63±0.02 vs 0.57±0.04,P<0.05);受精后胚胎发育方面,I液和II液组的卵裂率差异不显著(0.79±0.02 vs 0.81±0.02,P>0.05);然而II液组桑葚胚率显著高于I液组(0.71±0.02 vs 0.65±0.03,P<0.05)。总之,II液对绵羔羊卵子体外成熟、受精及发育的支持能力较好。此外,本研究进一步指出,II液中的半胱酰胺和丙酮酸钠可能是使其具有较强支持绵羔羊卵子成熟、发育能力的主要原因。
