JNK是MAPK超家族成员之一,c-Jun是JNK的主要下游因子,是一种受JNK调控的即早基因。JNK和c-Jun是创伤、应激、细胞凋亡相关的调节因子,参与调控多种疾病的发生发展过程。近年来,研究发现,JNK/c-Jun信号通路在IgA肾病、抗GBM肾小球肾炎、...JNK是MAPK超家族成员之一,c-Jun是JNK的主要下游因子,是一种受JNK调控的即早基因。JNK和c-Jun是创伤、应激、细胞凋亡相关的调节因子,参与调控多种疾病的发生发展过程。近年来,研究发现,JNK/c-Jun信号通路在IgA肾病、抗GBM肾小球肾炎、肾纤维化、急性肾损伤等多种肾脏疾病中表现为异常活化,调控着相关肾脏疾病的发生和发展过程。本文就JNK/c-Jun信号通路在肾脏疾病发生发展过程中的调控作用作简要综述。JNK is one of the members of the MAPK superfamily, and c-Jun is the main downstream factor of JNK, which is an early gene regulated by JNK. JNK and c-Jun are regulators related to trauma, stress and apoptosis, and are involved in regulating the occurrence and development of a variety of diseases. In recent years, studies have found that the JNK/c-Jun signaling pathway is abnormally activated in a variety of kidney diseases, such as IgA nephropathy, anti-GBM glomerulonephritis, renal fibrosis, and acute kidney injury, which regulates the occurrence and development of related kidney diseases. This article briefly reviews the regulatory role of JNK/c-Jun signaling pathway in the occurrence and development of kidney diseases.展开更多
丝裂原活化蛋白激酶(mitogen-activated protein kinase,MAPK)是广泛表达的丝氨酸/酪氨酸激酶,在哺乳动物细胞多种信号转导通路中起重要作用。MAPK有4个主要亚族:ERK、JNK、p38MAPK和ERK5。JNK信号转导通路是MAPK通路的一重要分支,它在...丝裂原活化蛋白激酶(mitogen-activated protein kinase,MAPK)是广泛表达的丝氨酸/酪氨酸激酶,在哺乳动物细胞多种信号转导通路中起重要作用。MAPK有4个主要亚族:ERK、JNK、p38MAPK和ERK5。JNK信号转导通路是MAPK通路的一重要分支,它在细胞周期、生产、凋亡和细胞应激等多种生理和病理过程中起重要作用。本文就JNK的分型、分布、信号通路的组成及其在组织损伤中的作用进行综述,旨在为组织损伤程度和伤后时间推断的法医病理学研究提供参考资料。展开更多
文摘JNK是MAPK超家族成员之一,c-Jun是JNK的主要下游因子,是一种受JNK调控的即早基因。JNK和c-Jun是创伤、应激、细胞凋亡相关的调节因子,参与调控多种疾病的发生发展过程。近年来,研究发现,JNK/c-Jun信号通路在IgA肾病、抗GBM肾小球肾炎、肾纤维化、急性肾损伤等多种肾脏疾病中表现为异常活化,调控着相关肾脏疾病的发生和发展过程。本文就JNK/c-Jun信号通路在肾脏疾病发生发展过程中的调控作用作简要综述。JNK is one of the members of the MAPK superfamily, and c-Jun is the main downstream factor of JNK, which is an early gene regulated by JNK. JNK and c-Jun are regulators related to trauma, stress and apoptosis, and are involved in regulating the occurrence and development of a variety of diseases. In recent years, studies have found that the JNK/c-Jun signaling pathway is abnormally activated in a variety of kidney diseases, such as IgA nephropathy, anti-GBM glomerulonephritis, renal fibrosis, and acute kidney injury, which regulates the occurrence and development of related kidney diseases. This article briefly reviews the regulatory role of JNK/c-Jun signaling pathway in the occurrence and development of kidney diseases.
文摘目的 基于c-Jun氨基末端激酶(JNK)-p62/螯合体(SQSTM1)信号通路探讨糖肾煎对2型糖尿病肾病(DN)大鼠足细胞的保护作用。方法 SD大鼠随机分成正常组、DN组、糖肾煎低、中、高[生药5、10、20 g/(kg·d)]剂量组(糖肾煎-L、M、H组)、二甲双胍组[100 mg/(kg·d)]。除正常组外,其余各组通过喂养高脂高糖饲料和腹腔注射链脲佐菌素(STZ)进行DN模型构建。药物干预结束后,检测大鼠血生化指标空腹血糖(FBG)、负荷后2 h血糖(P2 h BG)、血肌酐(SCr)、血尿素氮(BUN)水平;苏木素-伊红(HE)、六胺银(PASM)染色观察肾组织病理学变化;透射电镜(TEM)观察肾小球基底膜损伤和足细胞变化情况;Western印迹检测肾组织中微管相关蛋白1A/1B-轻链(LC)3、p-JNK、JNK、p62/SQSTM1、肾病蛋白(Nephrin)蛋白表达。结果 与正常组比较,DN组FBG、P2 h BG、SCr、BUN水平及p62/SQSTM1蛋白表达明显升高,LC3-Ⅱ、Nephrin蛋白表达和p-JNK/JNK明显降低(P<0.05);光镜下观察到肾小球缩小、管丛系膜明显扩张,并有基底膜增生增厚等现象;TEM下观察到肾小球基底膜增厚、足细胞排列紊乱、形态改变、足突融合等现象。与DN组比较,糖肾煎-L、M、H组和二甲双胍组FBG、P2 h BG、SCr、BUN水平及p62/SQSTM1蛋白表达明显降低,LC3-Ⅱ、Nephrin蛋白表达和p-JNK/JNK明显升高(P<0.05);并且肾小球基底膜增厚、足细胞足突融合等情况均获得一定程度减轻。结论 糖肾煎对2型DN大鼠足细胞具有一定保护作用,可能是通过调控JNK-p62/SQSTM1信号通路,提高足细胞自噬,从而起到肾脏保护功效。
文摘丝裂原活化蛋白激酶(mitogen-activated protein kinase,MAPK)是广泛表达的丝氨酸/酪氨酸激酶,在哺乳动物细胞多种信号转导通路中起重要作用。MAPK有4个主要亚族:ERK、JNK、p38MAPK和ERK5。JNK信号转导通路是MAPK通路的一重要分支,它在细胞周期、生产、凋亡和细胞应激等多种生理和病理过程中起重要作用。本文就JNK的分型、分布、信号通路的组成及其在组织损伤中的作用进行综述,旨在为组织损伤程度和伤后时间推断的法医病理学研究提供参考资料。
