Objective: To investigate protective effects of Hydnophytum formicarum Jack.(H. formicarum) extracts via regulation of SIRT1-FOXO3a-ADAM10 signaling and antioxidant activity against H_2O_2-induced neurotoxicity in neu...Objective: To investigate protective effects of Hydnophytum formicarum Jack.(H. formicarum) extracts via regulation of SIRT1-FOXO3a-ADAM10 signaling and antioxidant activity against H_2O_2-induced neurotoxicity in neuroblastoma SH-SY5Y cells. Methods: Cell viability and apoptosis of neuronal cells pretreated with H. formicarum Jack. extracts under oxidative stress were determined by MTT assay and flow cytometry. The intracellular reactive oxygen species(ROS) was performed using Carboxy-DCFDA assay. Additionally, a profile of protein expressions related to neuroprotection was detected by western blot analysis. Results: The plant extracts(methanol and ethyl acetate) elicited protective effects on the neuronal cell death as performed by the MTT assay and by apoptosis analysis via the activation of BCL-2. Both ethyl acetate and methanol extracts exerted inhibitory effects against H_2O_2-induced ROS generation in the SH-SY5Y cells. Furthermore, the possible mechanism of neuroprotection of H. formicarum Jack. was observed through its antioxidant properties by maintaining the levels of catalase and SOD2 proteins as well as activating SIRT1-FOXO3a pathway. Importantly, pretreatment of neuronal cells with H. formicarum Jack. significantly recovered the levels of ADAM10 protein compared with the H_2O_2 treatment alone. Conclusions: The recent findings suggest the protective effects of H. formicarum Jack. plant extracts on attenuating H_2O_2-induced neurotoxicity in human SH-SY5Y cells.展开更多
The twigs and leaves of Pithecellobium clypearia(Jack.)Benth are used as common Chinese traditional herbal medicine to treat upper respiratory tract infection,acute gastroenteritis and bacterial dysentery.The study is...The twigs and leaves of Pithecellobium clypearia(Jack.)Benth are used as common Chinese traditional herbal medicine to treat upper respiratory tract infection,acute gastroenteritis and bacterial dysentery.The study isolated nine compounds from the EtOAc extract,all of which were obtained from Pithecellobium Mart.for the first time.Based on 1H-NMR and 13C-NMR data,the structures of these compounds,including two ladiosiplodins and two dihydroisocoumarins,were elucidated.展开更多
Traditional construction techniques have a significant impact on the environment and are associated with long construction durations in the construction of municipal rainwater pipelines.Pipe jacking technology,a new t...Traditional construction techniques have a significant impact on the environment and are associated with long construction durations in the construction of municipal rainwater pipelines.Pipe jacking technology,a new type of pipeline construction method,enables non-excavation construction and can address the shortcomings of traditional pipeline construction.This article analyzes the concept and application advantages of pipe jacking technology.Combining engineering examples,it explores the application strategies of pipe jacking technology in the construction process of municipal rainwater pipelines for reference.展开更多
在驾驶舱的设计中应用人机工效学原理,利用JACK软件对舱内布局设计进行仿真及舒适性、可达域、可视域等工效进行评价,并提出修改意见。首先将基于人机工效学设计的驾驶舱的CATIA模型导入JACK软件中创建分析对象;然后基于JACK软件建立用...在驾驶舱的设计中应用人机工效学原理,利用JACK软件对舱内布局设计进行仿真及舒适性、可达域、可视域等工效进行评价,并提出修改意见。首先将基于人机工效学设计的驾驶舱的CATIA模型导入JACK软件中创建分析对象;然后基于JACK软件建立用于仿真工效分析的数字人模型(Digital Human Model),将数字人模型调整成驾驶员工作时的最优姿势,仿真驾驶员位于驾驶舱的虚拟环境。最后应用JACK软件的人机工效分析工具对驾驶舱内部进行分析并提出改进意见,这样既缩短了设计周期,又减少了设计制造费用。展开更多
文摘Objective: To investigate protective effects of Hydnophytum formicarum Jack.(H. formicarum) extracts via regulation of SIRT1-FOXO3a-ADAM10 signaling and antioxidant activity against H_2O_2-induced neurotoxicity in neuroblastoma SH-SY5Y cells. Methods: Cell viability and apoptosis of neuronal cells pretreated with H. formicarum Jack. extracts under oxidative stress were determined by MTT assay and flow cytometry. The intracellular reactive oxygen species(ROS) was performed using Carboxy-DCFDA assay. Additionally, a profile of protein expressions related to neuroprotection was detected by western blot analysis. Results: The plant extracts(methanol and ethyl acetate) elicited protective effects on the neuronal cell death as performed by the MTT assay and by apoptosis analysis via the activation of BCL-2. Both ethyl acetate and methanol extracts exerted inhibitory effects against H_2O_2-induced ROS generation in the SH-SY5Y cells. Furthermore, the possible mechanism of neuroprotection of H. formicarum Jack. was observed through its antioxidant properties by maintaining the levels of catalase and SOD2 proteins as well as activating SIRT1-FOXO3a pathway. Importantly, pretreatment of neuronal cells with H. formicarum Jack. significantly recovered the levels of ADAM10 protein compared with the H_2O_2 treatment alone. Conclusions: The recent findings suggest the protective effects of H. formicarum Jack. plant extracts on attenuating H_2O_2-induced neurotoxicity in human SH-SY5Y cells.
文摘The twigs and leaves of Pithecellobium clypearia(Jack.)Benth are used as common Chinese traditional herbal medicine to treat upper respiratory tract infection,acute gastroenteritis and bacterial dysentery.The study isolated nine compounds from the EtOAc extract,all of which were obtained from Pithecellobium Mart.for the first time.Based on 1H-NMR and 13C-NMR data,the structures of these compounds,including two ladiosiplodins and two dihydroisocoumarins,were elucidated.
文摘Traditional construction techniques have a significant impact on the environment and are associated with long construction durations in the construction of municipal rainwater pipelines.Pipe jacking technology,a new type of pipeline construction method,enables non-excavation construction and can address the shortcomings of traditional pipeline construction.This article analyzes the concept and application advantages of pipe jacking technology.Combining engineering examples,it explores the application strategies of pipe jacking technology in the construction process of municipal rainwater pipelines for reference.
文摘在驾驶舱的设计中应用人机工效学原理,利用JACK软件对舱内布局设计进行仿真及舒适性、可达域、可视域等工效进行评价,并提出修改意见。首先将基于人机工效学设计的驾驶舱的CATIA模型导入JACK软件中创建分析对象;然后基于JACK软件建立用于仿真工效分析的数字人模型(Digital Human Model),将数字人模型调整成驾驶员工作时的最优姿势,仿真驾驶员位于驾驶舱的虚拟环境。最后应用JACK软件的人机工效分析工具对驾驶舱内部进行分析并提出改进意见,这样既缩短了设计周期,又减少了设计制造费用。
