In this study,voltammetric and spectroscopic investigation of the interaction between Janus Green B（JGB） and bovine serum albumin（BSA） was reported.The interaction was observed at Britton-Robinson buffer（pH 7.0）...In this study,voltammetric and spectroscopic investigation of the interaction between Janus Green B（JGB） and bovine serum albumin（BSA） was reported.The interaction was observed at Britton-Robinson buffer（pH 7.0）.When JGB was added to solution containing BSA,the peak currents of BSA decrease with the increasing of JGB concentrations which is due to the interaction of JGB and BSA.The binding constant of JGB with BSA was obtained by voltammetric data.Also,this interaction was supported by means of UV-vis spectroscopic measurements.The UV-vis absorption spectra of JGB in the presence of BSA decrease with the increasing of BSA concentrations.展开更多
This study describes a simple,rapid and selective catalytic kinetic spectrophotometric method for the determination of 6- monoacetylmorphine（6-MAM） as major metabolite of heroin in biological samples.The method is b...This study describes a simple,rapid and selective catalytic kinetic spectrophotometric method for the determination of 6- monoacetylmorphine（6-MAM） as major metabolite of heroin in biological samples.The method is based upon the catalytic effect of 6-MAM on the oxidation of Janus Green by bromate in acid media.The reaction was followed spectrophotometrically by measuring the decrease in absorbance of Janus Green at 618 nm.The dependence of sensitivity on the reaction variables was studied.Under optimum conditions,two linear calibration curves over the range 0.1-1.0μg mL^（-1） and 1.0-34.0μg mL^（-1） of 6- MAM were obtained.The detection limit was 1.2×10~2μg mL^（-1） of 6-MAM.The relative standard deviations for six replicate determinations of 0.8 and 5.0μg mL^（-1） of 6-MAM were 1.4 and 1.1%respectively.The effect of various species commonly associated with heroin in real samples was also investigated.The proposed method was successfully applied in human urine and serum samples with satisfactory results.展开更多
文摘In this study,voltammetric and spectroscopic investigation of the interaction between Janus Green B（JGB） and bovine serum albumin（BSA） was reported.The interaction was observed at Britton-Robinson buffer（pH 7.0）.When JGB was added to solution containing BSA,the peak currents of BSA decrease with the increasing of JGB concentrations which is due to the interaction of JGB and BSA.The binding constant of JGB with BSA was obtained by voltammetric data.Also,this interaction was supported by means of UV-vis spectroscopic measurements.The UV-vis absorption spectra of JGB in the presence of BSA decrease with the increasing of BSA concentrations.
文摘This study describes a simple,rapid and selective catalytic kinetic spectrophotometric method for the determination of 6- monoacetylmorphine（6-MAM） as major metabolite of heroin in biological samples.The method is based upon the catalytic effect of 6-MAM on the oxidation of Janus Green by bromate in acid media.The reaction was followed spectrophotometrically by measuring the decrease in absorbance of Janus Green at 618 nm.The dependence of sensitivity on the reaction variables was studied.Under optimum conditions,two linear calibration curves over the range 0.1-1.0μg mL^（-1） and 1.0-34.0μg mL^（-1） of 6- MAM were obtained.The detection limit was 1.2×10~2μg mL^（-1） of 6-MAM.The relative standard deviations for six replicate determinations of 0.8 and 5.0μg mL^（-1） of 6-MAM were 1.4 and 1.1%respectively.The effect of various species commonly associated with heroin in real samples was also investigated.The proposed method was successfully applied in human urine and serum samples with satisfactory results.
