To investigate the effects of silicon formulations on the cold tolerance of rice seedlings,Song Japonica 16(not cold tolerant)and Dongnong 427(cold tolerant)rice varieties were used as test materials and four differen...To investigate the effects of silicon formulations on the cold tolerance of rice seedlings,Song Japonica 16(not cold tolerant)and Dongnong 427(cold tolerant)rice varieties were used as test materials and four different types of silicon formulations,Si-50-G,Si-60-G,Si-T-G,and Si-E-G,were applied as foliar sprays at the seedling stage,and a control group CK(equal amount of distilled water)was set up.One week after the first silicon spray,two types of rice were subjected to low-temperature stress treatments at day/night temperatures of 12℃/10℃for 2,4,6,and 8 days.The effects of different silicon formulations on the chlorophyll,proline(Pro)and soluble sugar contents as well as superoxide dismutase(SOD),peroxidase(POD)and catalase(CAT)activities of rice seedlings under low-temperature stress were compared to find out the effects of silicon formulations on the cold tolerance of rice seedlings.The results showed that silicon formulations could significantly increase the chlorophyll content of rice seedling leaves,with Si-50-G being the most effective,with a significant increment of 40.17%compared to the CK at 2 days of low temperature.Four silicon formulations significantly increased the proline content and soluble sugar content of rice leaves at low temperature for 4-8 days.For Song Japonica 16,the most significant increment in leaf POD activity was observed in Si-E-G treatment at 2,4 and 8 days of temperature stress,with 73.58%,20.95%and 217.24%increases compared to the CK,respectively.For 4 and 6 days of temperature stress,the most significant increase in CAT activity was observed in Si-E-G treatment,with 25.70%and 75.78%increases compared to the CK,respectively.For Dongnong 427,the Si-60-G treatment showed the highest increase in leaf SOD activity for 4 and 8 days of temperature stress,with significant increases of 58.15%and 82.76%compared to the CK,respectively,and the Si-E-G treatment showed the highest increase in leaf POD activity for 2 and 8 days of temperature stress,with significant increases of 97.75%and 245.10%compared to the CK,respectively.It showed that the spraying of silicon formulations could significantly enhance the cold tolerance of rice.This study provided a scientific basis for the rational use of silicon formulations to enhance cold tolerance in rice and had important theoretical and practical significance for ensuring sustainable high and stable rice yields in Heilongjiang Province,as well as for the development of silicon fertilizers.展开更多
In the Yangtze river aera,the first crop of indi-ca rice is sown in late Mar to early Apr andtransplanted in early May.Usually,seedlingsperish when abnormal low temperature of 6-10℃ lasted for 3 d or longer.The effec...In the Yangtze river aera,the first crop of indi-ca rice is sown in late Mar to early Apr andtransplanted in early May.Usually,seedlingsperish when abnormal low temperature of 6-10℃ lasted for 3 d or longer.The effect ofsocking seed with urea solution on increasingthe cold tolerance at the seedling stage was展开更多
The quantitative trait loci (QTLs) for cold tolerance relative characters were identified with microsatellite markers.Ten QTLs located on chromosome 1, 3, 4, 5, 6, 8, 9, 11(two)and 12 were detected for seedling height...The quantitative trait loci (QTLs) for cold tolerance relative characters were identified with microsatellite markers.Ten QTLs located on chromosome 1, 3, 4, 5, 6, 8, 9, 11(two)and 12 were detected for seedling height at different low temperature. Only 2 of these were detected at the same locus at four environments, 1 was significant at three environments, 6 were significant at two environments and 1 was significant at one environment. Seven QTLs located on chromosome 1(two), 2(two),5,6,8 were found for low temperature chlorosis resistance and five QTLs located on chromosome 3, 4, 7, 8, 11 resistant to chilling injury. The amount of variation explained by individual QTL ranged from 4.85%to 49.34%. There was no linkage relationship among the three characters, which indicates seedling cold tolerance is a complex character and is controlled by different QTLs.展开更多
A doubled haploid population, derived from anther culture of F hybrid between a typical indica cv. and a japonica cv. has been used to investigate the seedling cold tolerance (SCT) in growth cabinet. By dynamically an...A doubled haploid population, derived from anther culture of F hybrid between a typical indica cv. and a japonica cv. has been used to investigate the seedling cold tolerance (SCT) in growth cabinet. By dynamically analyzing every day’s survival percentages of the parents and DH lines under 7-d cold plus 9-d normal temperature condition, the guantitative trait loci (QTLs) for SCT have been mapped based on a molecular linkage map constructed from this population. The results show that two parents had significant differences in SCT and the segregation of SCT in DH lines was basically a continuous distribution with most serious injury on the 6th d of the cold treatment. A total of 4 QTLs for SCT have been identified on chromosomes 1, 2, 3 and 4 respectively. The additive effects of qSCT-1, qSCT-2 and qSCT-3 have been contributed by the japonica ev JX17, but that of qSCT-4 has been contributed by the indica cv ZYQ8. The mechanism of SCT seems complicated since the above 4 QTLs detected at different stages展开更多
M202, IR50 and advanced backcross （BC4F6） lines carrying the cold tolerance QTLs qCTS4 and qCTS12 were evaluated using three low temperature stress assays （constant, intermittent and constant with recovery period）...M202, IR50 and advanced backcross （BC4F6） lines carrying the cold tolerance QTLs qCTS4 and qCTS12 were evaluated using three low temperature stress assays （constant, intermittent and constant with recovery period）. Under constant stress, two BC4F6 lines （MIb 4853-9 and MIb 6885-2） exhibited differences in seedling growth and accumulation of stress-related compounds although both carry the two QTLs in the IRS0 background. Differences between these lines were also observed in the constant with recovery period assay, although both of them performed comparably under intermittent stress, MIb 6885-2 performed more similarly to M202 in the constant and constant with recovery period assays, suggesting that this line contains an introgressed region（s） not present in MIb 4853-9. The three assays were also applied to assess variations in cold tolerance in a set of diverse germplasms. Performance metrics and visual ratings were comparable for evaluating tolerance to constant stress. However, differences in response to intermittent stress were more evident from growth and total chlorophyll measurements than from visual ratings. With regard to the constant with recovery period assay, about 15% of the germplasm accessions did not recover. However, some lines which exhibited chilling injuries of comparable severity were able to overcome them. This recovery phenotype may improve rice performance in the field and warrant further investigation.展开更多
基金the National Natural Science Foundation of China(31471440)。
文摘To investigate the effects of silicon formulations on the cold tolerance of rice seedlings,Song Japonica 16(not cold tolerant)and Dongnong 427(cold tolerant)rice varieties were used as test materials and four different types of silicon formulations,Si-50-G,Si-60-G,Si-T-G,and Si-E-G,were applied as foliar sprays at the seedling stage,and a control group CK(equal amount of distilled water)was set up.One week after the first silicon spray,two types of rice were subjected to low-temperature stress treatments at day/night temperatures of 12℃/10℃for 2,4,6,and 8 days.The effects of different silicon formulations on the chlorophyll,proline(Pro)and soluble sugar contents as well as superoxide dismutase(SOD),peroxidase(POD)and catalase(CAT)activities of rice seedlings under low-temperature stress were compared to find out the effects of silicon formulations on the cold tolerance of rice seedlings.The results showed that silicon formulations could significantly increase the chlorophyll content of rice seedling leaves,with Si-50-G being the most effective,with a significant increment of 40.17%compared to the CK at 2 days of low temperature.Four silicon formulations significantly increased the proline content and soluble sugar content of rice leaves at low temperature for 4-8 days.For Song Japonica 16,the most significant increment in leaf POD activity was observed in Si-E-G treatment at 2,4 and 8 days of temperature stress,with 73.58%,20.95%and 217.24%increases compared to the CK,respectively.For 4 and 6 days of temperature stress,the most significant increase in CAT activity was observed in Si-E-G treatment,with 25.70%and 75.78%increases compared to the CK,respectively.For Dongnong 427,the Si-60-G treatment showed the highest increase in leaf SOD activity for 4 and 8 days of temperature stress,with significant increases of 58.15%and 82.76%compared to the CK,respectively,and the Si-E-G treatment showed the highest increase in leaf POD activity for 2 and 8 days of temperature stress,with significant increases of 97.75%and 245.10%compared to the CK,respectively.It showed that the spraying of silicon formulations could significantly enhance the cold tolerance of rice.This study provided a scientific basis for the rational use of silicon formulations to enhance cold tolerance in rice and had important theoretical and practical significance for ensuring sustainable high and stable rice yields in Heilongjiang Province,as well as for the development of silicon fertilizers.
文摘In the Yangtze river aera,the first crop of indi-ca rice is sown in late Mar to early Apr andtransplanted in early May.Usually,seedlingsperish when abnormal low temperature of 6-10℃ lasted for 3 d or longer.The effect ofsocking seed with urea solution on increasingthe cold tolerance at the seedling stage was
基金funded by the project 2006 NG 04 of Yunnan Province,Chinathe International corporation project between the Yunnan Academy of Agricultural Sciences of China and the National Institute of Crop Science,Rural Development Administration of Korea
文摘The quantitative trait loci (QTLs) for cold tolerance relative characters were identified with microsatellite markers.Ten QTLs located on chromosome 1, 3, 4, 5, 6, 8, 9, 11(two)and 12 were detected for seedling height at different low temperature. Only 2 of these were detected at the same locus at four environments, 1 was significant at three environments, 6 were significant at two environments and 1 was significant at one environment. Seven QTLs located on chromosome 1(two), 2(two),5,6,8 were found for low temperature chlorosis resistance and five QTLs located on chromosome 3, 4, 7, 8, 11 resistant to chilling injury. The amount of variation explained by individual QTL ranged from 4.85%to 49.34%. There was no linkage relationship among the three characters, which indicates seedling cold tolerance is a complex character and is controlled by different QTLs.
文摘A doubled haploid population, derived from anther culture of F hybrid between a typical indica cv. and a japonica cv. has been used to investigate the seedling cold tolerance (SCT) in growth cabinet. By dynamically analyzing every day’s survival percentages of the parents and DH lines under 7-d cold plus 9-d normal temperature condition, the guantitative trait loci (QTLs) for SCT have been mapped based on a molecular linkage map constructed from this population. The results show that two parents had significant differences in SCT and the segregation of SCT in DH lines was basically a continuous distribution with most serious injury on the 6th d of the cold treatment. A total of 4 QTLs for SCT have been identified on chromosomes 1, 2, 3 and 4 respectively. The additive effects of qSCT-1, qSCT-2 and qSCT-3 have been contributed by the japonica ev JX17, but that of qSCT-4 has been contributed by the indica cv ZYQ8. The mechanism of SCT seems complicated since the above 4 QTLs detected at different stages
文摘M202, IR50 and advanced backcross （BC4F6） lines carrying the cold tolerance QTLs qCTS4 and qCTS12 were evaluated using three low temperature stress assays （constant, intermittent and constant with recovery period）. Under constant stress, two BC4F6 lines （MIb 4853-9 and MIb 6885-2） exhibited differences in seedling growth and accumulation of stress-related compounds although both carry the two QTLs in the IRS0 background. Differences between these lines were also observed in the constant with recovery period assay, although both of them performed comparably under intermittent stress, MIb 6885-2 performed more similarly to M202 in the constant and constant with recovery period assays, suggesting that this line contains an introgressed region（s） not present in MIb 4853-9. The three assays were also applied to assess variations in cold tolerance in a set of diverse germplasms. Performance metrics and visual ratings were comparable for evaluating tolerance to constant stress. However, differences in response to intermittent stress were more evident from growth and total chlorophyll measurements than from visual ratings. With regard to the constant with recovery period assay, about 15% of the germplasm accessions did not recover. However, some lines which exhibited chilling injuries of comparable severity were able to overcome them. This recovery phenotype may improve rice performance in the field and warrant further investigation.
