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Study on Establishment of cpSSR Marker Technology and Optimization of Its Reaction System in Jatropha curcas Linn 被引量:1
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作者 向倩 周兰英 +6 位作者 万静 张旭 雷宝盛 金银春 冯毅 于绪任 赵晓英 《Agricultural Science & Technology》 CAS 2009年第4期61-64,74,共5页
[ Objective] The aim of this study was to establish the optimum cpSSR-PCR system for Jatropha curcas Linn. [ Method] cpSSR-PCR amplification system for Jatropha curcas Linn influenced by five factors including Taq DNA... [ Objective] The aim of this study was to establish the optimum cpSSR-PCR system for Jatropha curcas Linn. [ Method] cpSSR-PCR amplification system for Jatropha curcas Linn influenced by five factors including Taq DNA polymerase, Mg^2+ , DNA template, dNTP and primer were optimized from several levels. [ Result] The optimum concentration of 20 μl reaction system was 10 × Buffer, 2.00 mmol/L Mg^2+ , 2 U/μl Taq DNA polymerase, 0.2 mmol/L dNTP, 0.2 μmol/L primer and 35 ng/μl DNA template. [ Conclusion] The optimum annealing temperature for cpSSR-PCR reaction system is 52 ℃, and the cpSSR reaction system is steady and reproducible. 展开更多
关键词 jatropha curcas linn cpSSR-PCR Optimization of reaction system
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Development and Characterization of Eco-Friendly Non-Isocyanate Urethane Monomer from Jatropha curcas Oil for Wood Composite Applications 被引量:2
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作者 Samsul Bhakri Muhammad Ghozali +10 位作者 Edy Cahyono Evi Triwulandari Witta Kartika Restu Nissa Nurfajrin Solihat Apri Heri Iswanto Petar Antov Viktor Savov Lee Seng Hua ErikaAyu Agustiany Lubos Kristak Widya Fatriasari 《Journal of Renewable Materials》 SCIE EI 2023年第1期41-59,共19页
The aim of this research work was to evaluate the potential of using renewable natural feedstock,i.e.,Jatropha curcas oil(JCO)for the synthesis of non-isocyanate polyurethane(NIPU)resin for wood composite applications... The aim of this research work was to evaluate the potential of using renewable natural feedstock,i.e.,Jatropha curcas oil(JCO)for the synthesis of non-isocyanate polyurethane(NIPU)resin for wood composite applications.Commercial polyurethane(PU)is synthesized through a polycondensation reaction between isocyanate and poly-ol.However,utilizing toxic and unsustainable isocyanates for obtaining PU could contribute to negative impacts on the environment and human health.Therefore,the development of PU from eco-friendly and sustainable resources without the isocyanate route is required.In this work,tetra-n-butyl ammonium bromide was used as the activator to open the epoxy ring with 3-Aminopropyltriethoxisylane as a catalyst to yield urethane of JCO(UJCO).The UJCO were characterized by Fourier Transform Infra-Red spectroscopy(FTIR)and their oxirane,and hydroxyl values were measured.The result showed that a decrease in oxirane value was found while the hydroxyl value was increased during the time,confirming that the urethane group was formed.The presence of functional groups in FTIR spectra at wave numbers 1732.08,1562.34,and 3348.42 cm^(−1) indicates the functional groups of C=O(urethane carbonyl),–NH,and–OH,respectively confirmed this finding.The potential applications of NIPU in the wood composite were also outlined. 展开更多
关键词 jatropha curcas oil urethane groups non-isocyanate polyurethane epoxidized JCO cyclic carbonate oxirane value wood composites
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Effect of Water Deficit Stress on Photosynthetic Characteristics of Jatropha curcas
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作者 Kesego Makholwa Baleseng Moseki Goitseone Malambane 《American Journal of Plant Sciences》 CAS 2023年第1期104-112,共9页
The need to mitigate climate change cannot be more emphasized, which arises, as a result of increases in CO<sub>2</sub> emissions due to anthropogenic activities. Given the current world energy problems of... The need to mitigate climate change cannot be more emphasized, which arises, as a result of increases in CO<sub>2</sub> emissions due to anthropogenic activities. Given the current world energy problems of high fossil fuel consumption which plays a pivotal role in the greenhouse effect, Jatropha curcas biodiesel has been considered a potential alternative source of clean energy (biodiesel is carbon neutral). However, the ability of Jatropha curcas, as a candidate source of alternative of clean energy, to grow in marginal and dry soils, has been poorly elucidated. This study, therefore aimed at investigating whether Jatropha curcas leaves could switch from carrying out C<sub>3</sub> photosynthetic pathway to Crassulacean Acid Metabolism (CAM) as a strategy to improve its water deficit tolerance. Thirty-five-day-old Jatropha curcas accessions, from three different climatic zones of Botswana, viz., Mmadinare (Central zone), Thamaga (Southern zone) and Maun (Northern zone), were subjected to water stress, by with-holding irrigation with half-strength Hoagland culture solution. Net photosynthetic rate, transpiration and stomatal conductance were measured at weekly intervals. The leaf pH was measured to determine whether there was a decrease in pH (leaf acidification) of the leaves during the night, when the plants experienced water deficit stress. All the accessions exhibited marked reduction in all the measured photosynthetic characteristics when experience water deficit stress. However, a measurable CO<sub>2</sub> uptake was carried out by leaves of all the accessions, in the wake of marked decreases in stomatal conductance. There is evidence to suggest that when exposed to water stress J. curcas accessions switch from C<sub>3</sub> mode of photosynthesis to CAM photosynthetic pathway. This is attested to by the slightly low leaf pH at night. Thamaga accession exhibited an earlier stomatal closure than the other two accessions. This resulted in Thamaga accession displaying a slightly lower dry weight than both Mmadinare and Maun accessions. It could be concluded that Jatropha curcas appeared to tolerate water deficit stress due to its ability of switching from C<sub>3</sub> photosynthetic pathway to the CAM photosynthetic pathway, but with a cost to biomass accumulation, as demonstrated by slightly more reduced CO<sub>2</sub> assimilation by Thamaga accession, than the other two accessions. 展开更多
关键词 jatropha curcas BIODIESEL Crassulacean Acid Metabolism Net Photosynthetic Rate Stomatal Conductance
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麻疯树(Jatropha curcas L.)幼苗生长和光合作用对盐胁迫的响应 被引量:78
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作者 陈健妙 郑青松 +2 位作者 刘兆普 隆小华 刘联 《生态学报》 CAS CSCD 北大核心 2009年第3期1356-1365,共10页
为探讨盐胁迫对麻疯树幼苗生长和叶片光合生理的影响,在温室不同浓度NaCl处理下,对生长、光合色素含量、净光合速率Pn等光合参数、叶片MDA含量和电解质相对外渗率EL进行测定。结果表明:①25~50mmol·L-1NaCl处理促进幼苗生长,尤其... 为探讨盐胁迫对麻疯树幼苗生长和叶片光合生理的影响,在温室不同浓度NaCl处理下,对生长、光合色素含量、净光合速率Pn等光合参数、叶片MDA含量和电解质相对外渗率EL进行测定。结果表明:①25~50mmol·L-1NaCl处理促进幼苗生长,尤其是50mmol·L-1NaCl处理,鲜重较对照显著增加44.3%,100~150mmol·L-1NaCl对生长影响不大,200~250mmol·L-1NaCl使生长受抑制,鲜重较对照分别显著下降39.3%和70.2%。②50mmol·L-1NaCl时,Pn较对照显著增加17.2%,100~150mmol·L-1NaCl时与对照无显著差异,200~250mmol·L-1NaCl时分别比对照显著下降73.2%和77.9%,Gs和Tr呈相同趋势,MDA含量、EL则相反。③叶绿素Chl在25~50mmo.lL-1NaCl时递增,100~250mmol·L-1NaCl时递减,但Chla/Chlb值变化小;类胡萝卜素Car在25mmol·L-1NaCl时显著增加,100mmol·L-1NaCl后缓慢下降,但Car/Chl值呈上升趋势。④25~150mmo.lL-1NaCl时幼苗水分利用效率WUE与对照无显著差异,200~250mmol·L-1NaCl时比对照显著下降。综上所述,25~50mmol·L-1NaCl时,麻疯树幼苗通过增加气孔开张、增强光合膜等细胞膜稳定性和膜功能,使Pn显著增加,促进植株生长和提高耐盐性,100~150mmo.lL-1NaCl时,通过稳定的WUE使Pn下降不显著,生长受影响小,具有较好的耐盐性,200~250mmo.lL-1NaCl时,光合膜等生物膜功能减弱,使Pn显著下降,幼苗诱导活性氧清除系统并通过减小生长来提高耐盐性,具有较好的耐盐适应性。150mmol·L-1NaCl以下是麻疯树幼苗生长的适宜浓度。 展开更多
关键词 麻疯树 盐胁迫 光合 色素 细胞膜 水分利用效率
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云南麻疯树(Jatropha curcas)资源生态地理分布及评价 被引量:38
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作者 袁理春 赵琪 +8 位作者 康平德 杨丽云 赵菊 苟平 武奎 杨方旭 杨兴汝 李文昌 范源洪 《西南农业学报》 CSCD 2007年第6期1283-1286,共4页
对云南省麻疯树的分布区、资源量和粗脂肪含量开展系统调查研究。结果表明:云南麻疯树资源分布在金沙江、澜沧江、怒江和红河流域的260乡(镇),呈零星分布,不同产地种子粗脂肪含量差异较大,单株间亦存在差异;81个种群所采种仁中,永仁县... 对云南省麻疯树的分布区、资源量和粗脂肪含量开展系统调查研究。结果表明:云南麻疯树资源分布在金沙江、澜沧江、怒江和红河流域的260乡(镇),呈零星分布,不同产地种子粗脂肪含量差异较大,单株间亦存在差异;81个种群所采种仁中,永仁县永兴乡种仁粗脂肪含量最高达67.00%,屏边白河最低为34.88%。评述了云南省的小桐子资源状况,并术其资源及利用的价值优势,论述了云南麻疯树的开发利用前景。 展开更多
关键词 麻疯树 资源 云南 分布 评价
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Cloning and Expression of Curcin, a Ribosome-Inactivating Protein from the Seeds of Jatropha curcas 被引量:7
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作者 林娟 陈钰 +3 位作者 徐莺 颜钫 唐琳 陈放 《Acta Botanica Sinica》 CSCD 2003年第7期858-863,共6页
Curcin, a ribosome-inactivating protein with a molecular weight of about 28.2 kD, which strongly inhibits the protein synthesis in rabbit reticulocyte lysate system with an IC50 value of about (0.19 +/- 0.01) nmol/L, ... Curcin, a ribosome-inactivating protein with a molecular weight of about 28.2 kD, which strongly inhibits the protein synthesis in rabbit reticulocyte lysate system with an IC50 value of about (0.19 +/- 0.01) nmol/L, was purified from the seeds of Jatropha curcas L. The protein has the activity of rRNA N-glycosidase. Degenerate primers were designed based on the N-terminal partial sequence from purified curcin. The full-length curcin cDNA by RT-PCR and 5'-RACE was cloned. The deduced amino acids sequence indicates that a preprotein with 20 amino acid residues is first translated and then processed to a mature protein with 251 amino acids. The deduced amino acids sequence shares homology of 33% and 57% to those of type I ribosome-inactivating proteins (RIPs) and A chain of type II RIPs, respectively. The sequence encoding mature curcin was integrated into the pQE-30 vector for expression in Escherichia coli strain M15 (pREP4). The purified recombinant curcin was able to inhibit protein synthesis in rabbit reticulocyte lysate system. 展开更多
关键词 jatropha curcas CURCIN RNA N-glycosidase CLONING in Escherichia coli expression
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金沙江干热区麻疯树(Jatropha curcas L.)杂交育种技术的初步研究 被引量:6
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作者 何璐 张德 +7 位作者 段曰汤 陈艺齐 张磊 苟平 王耘 齐冸伦 李建忠 袁理春 《西南农业学报》 CSCD 北大核心 2012年第4期1422-1426,共5页
对金沙江干热区麻疯树生物特性、开花特性、结实特性进行观测,并开展不同地理种源麻疯树杂交育种试验。结果表明,麻疯树在本区域生长发育较好,可持续开花结果至1月初,雌雄花比例可达1∶10左右。通过麻疯树杂交育种试验,研究了麻疯树雌... 对金沙江干热区麻疯树生物特性、开花特性、结实特性进行观测,并开展不同地理种源麻疯树杂交育种试验。结果表明,麻疯树在本区域生长发育较好,可持续开花结果至1月初,雌雄花比例可达1∶10左右。通过麻疯树杂交育种试验,研究了麻疯树雌雄花分辨、雌花可授粉时间、套袋材料及去袋时间等特性。通过对金沙江干热区麻疯树主要特性和杂交育种技术试验研究,有利于提高育种效果,加速育种进程,提高育种工作的预见性。 展开更多
关键词 金沙江干热区 麻疯树 特性 杂交育种技术
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甲基磺酸乙酯(EMS)处理对麻疯树(Jatropha curcas L.)萌发种子的生理效应 被引量:7
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作者 徐刚 尹春英 牟东岭 《种子》 CSCD 北大核心 2010年第10期49-53,共5页
分析了不同甲基磺酸乙酯(EMS)浓度(0、0.1%、0.5%、1.0%、1.5%和2.0%)和在浓度为1.2%时不同处理时间(0、8、12、16h和20h)对麻疯树萌发种子的生理效应。结果表明,EMS对麻疯树种子的半致死浓度为1.2%,当浓度达到2.0%时,种子受到严重伤害... 分析了不同甲基磺酸乙酯(EMS)浓度(0、0.1%、0.5%、1.0%、1.5%和2.0%)和在浓度为1.2%时不同处理时间(0、8、12、16h和20h)对麻疯树萌发种子的生理效应。结果表明,EMS对麻疯树种子的半致死浓度为1.2%,当浓度达到2.0%时,种子受到严重伤害,成苗率为1.67%。用1.2%浓度诱变麻疯树种子,随着处理时间的延长,种子受到的伤害也随之加重,并且产生的矮化植株率也随之大幅度升高,在处理时间大于12h时,得到的植株都是矮化植株,但当处理时间达到20h时种子成苗率为0。由此看来,用EMS诱变麻疯树种子的合适浓度为1.2%,处理时间应选在8~12h范围内。 展开更多
关键词 麻疯树种子 甲基磺酸乙酯(EMS) 萌发和生长 生理效应
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金沙江干热区麻疯树(Jatropha curcas L.)白粉病危害特征观察 被引量:5
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作者 袁理春 罗艳苹 +1 位作者 卢恒 张艳 《西南农业学报》 CSCD 北大核心 2010年第4期1125-1127,共3页
在麻疯树的规模种植区选定代表性的10株无病植株,观察白粉病的危害侵染特征。结果显示:群居的麻疯树极易感染白粉病,在麻疯树的整个生长期都会受到不同程度的危害,8~10月高温高湿条件下是发病的高峰期,11月份以后减缓危害速度,但不停... 在麻疯树的规模种植区选定代表性的10株无病植株,观察白粉病的危害侵染特征。结果显示:群居的麻疯树极易感染白粉病,在麻疯树的整个生长期都会受到不同程度的危害,8~10月高温高湿条件下是发病的高峰期,11月份以后减缓危害速度,但不停止。结果对麻疯树白粉病预警预报和有效防控措施的制定提供参考依据。 展开更多
关键词 干热区 麻疯树 白粉病 危害特征
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麻疯树(Jatropha curcas L.)高含油量单株无性系筛选评价 被引量:2
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作者 袁理春 张艳 +1 位作者 罗艳苹 卢恒 《西南农业学报》 CSCD 北大核心 2010年第6期2034-2037,共4页
利用初步筛选出的种仁含油量大于65%的10个麻疯树地理种源单株,以种仁含油量为目标,在元谋干热河谷区,经过3次无性繁殖复选,并对其种仁含油量进行测定评价。结果表明:由于生长环境的改变,10个单株材料的种仁含油量都有不同程度的下降,... 利用初步筛选出的种仁含油量大于65%的10个麻疯树地理种源单株,以种仁含油量为目标,在元谋干热河谷区,经过3次无性繁殖复选,并对其种仁含油量进行测定评价。结果表明:由于生长环境的改变,10个单株材料的种仁含油量都有不同程度的下降,但来自元谋物茂乡、双柏大庄乡和云南永仁县的3个单株材料种仁含油量下降幅度较小,且连续表现稳定,可作为进一步品种选育和生产使用的无性系材料。 展开更多
关键词 麻疯树 单株 无性系筛选
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麻疯树(Jatropha curcas L.)种子总DNA提取方法的建立和优化 被引量:6
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作者 李静 曾德贤 +2 位作者 吴子欢 范林元 刘飞虎 《西南农业学报》 CSCD 北大核心 2011年第2期728-731,共4页
用种子提取DNA可省却培育幼苗过程而加快实验进度,为此建立了麻疯树(Jatropha curcas L.)种子DNA提取方法。针对麻疯树种子富含蛋白质、多酚及多糖等次生物质的特点,基于CTAB法进行优化,在研磨种子时加入抗氧化剂PVP去除多酚,接着用核... 用种子提取DNA可省却培育幼苗过程而加快实验进度,为此建立了麻疯树(Jatropha curcas L.)种子DNA提取方法。针对麻疯树种子富含蛋白质、多酚及多糖等次生物质的特点,基于CTAB法进行优化,在研磨种子时加入抗氧化剂PVP去除多酚,接着用核分离缓冲液去除多糖,再通过酚-氯仿-异戊醇(体积比=25∶24∶1)抽提去除蛋白质。所提取的麻疯树种子总DNA浓度和质量均较高,经琼脂糖凝胶电泳和ISSR-PCR扩增得到了非常清晰的DNA条带。 展开更多
关键词 麻疯树 种子DNA提取 改良CTAB法
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Changes of Water Status and Different Responses of Osmoregulants in Jatropha Curcas L. Seedlings to Air-drought Stress
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作者 陈凯 龚明 《Agricultural Science & Technology》 CAS 2011年第3期343-346,共4页
[Objective] The study aimed to investigate the changes of water status and different responses of osmoregulants during air-drought stress,to better understand mechanisms of drought resistance in Jatropha Curcas L. [Me... [Objective] The study aimed to investigate the changes of water status and different responses of osmoregulants during air-drought stress,to better understand mechanisms of drought resistance in Jatropha Curcas L. [Methods] The 12-day-old J. curcas seedlings were held in a climate chamber at 25/20 ℃(day/night),16 hours illumination,and 75% of relative humidity for air-drought treatment,and the changes of water potential,osmotic potential and the content of soluble sugar,proline,betaine were measured. [Results] Water potential and osmotic potential in leaves of J. curcas seedlings dropped significantly,pressure potential lost during air-drought stress,and the contents of osmoregulants soluble sugar,proline and betaine rose significantly to different extent in the leaves and stems. [Conclusion] Osmoregulants in the leaves and stems respond differently to air-drought stress,and in general leaves are much more responsive to the drought than stems of J. curcas seedlings. 展开更多
关键词 jatropha curcas L. Water status Osmoregulants Air-drought stress
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膏桐(Jatropha curcas)生物学研究进展 被引量:1
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作者 罗圆 欧晓昆 许继宏 《长江流域资源与环境》 CAS CSSCI CSCD 北大核心 2008年第A01期66-71,共6页
膏桐(Jatropha curcas)属大戟科麻疯树属多年生耐旱灌木或小乔木。因其生物柴油用途和生态发展潜力,受到世界各国政府及科学家的高度关注,其研究成为热点。膏桐理化性质、药用价值、致毒机理方面的研究较为完善,而膏桐生物学特别是其生... 膏桐(Jatropha curcas)属大戟科麻疯树属多年生耐旱灌木或小乔木。因其生物柴油用途和生态发展潜力,受到世界各国政府及科学家的高度关注,其研究成为热点。膏桐理化性质、药用价值、致毒机理方面的研究较为完善,而膏桐生物学特别是其生态学研究滞后且较少,对膏桐生态特性开展深入而全面的研究具有重要的科学和现实意义。综述了膏桐生物学领域的研究进展及成果,归纳了膏桐生物学研究中亟待解决的问题:膏桐种子生物学,自然群落膏桐种群建立、更新策略;论述了膏桐对生物多样性、群落稳定性、水土保持、物质循环以及化感作用潜在的影响;并在现有研究和相关理论的基础上展望了今后膏桐相关研究的发展方向:种间杂交、植被恢复、分布预测。 展开更多
关键词 膏桐 生物柴油 研究进展 生态功能
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Jatropha curcas L: Phytochemical, antimicrobial and larvicidal properties 被引量:5
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作者 Sillma Rampadarath Daneshwar Puchooa Rajesh Jeewon 《Asian Pacific Journal of Tropical Biomedicine》 SCIE CAS 2016年第10期858-865,共8页
Objective: To evaluate antimicrobial activities as well as the phytochemical and lavicidal properties of different parts of Jatropha curcas L.(J. curcas) growing in Mauritius.Methods: Determination of the presence of ... Objective: To evaluate antimicrobial activities as well as the phytochemical and lavicidal properties of different parts of Jatropha curcas L.(J. curcas) growing in Mauritius.Methods: Determination of the presence of phytochemicals in the crude plants extracts by test tube reactions. Disc diffusion method and microdilution method were used to detect the antimicrobial sensitivity and activity(minimal inhibitory concentration). The crude solvent extracts were also tested on the larvae of two insects, Bactrocera zonata and Bactrocera cucurbitae(Diptera, Tephritidae).Results: The antimicrobial activities were significantly dependent for the different crude plant extracts on the thirteen microorganisms tested. For the Gram-positive bacteria, the crude ethyl acetate extract was more efficient compared to the Gram-negative bacteria with both solvents being effective. The crude ethyl acetate extract of J. curcas bark and mature seed oil showed the highest efficacy. The highest mortality percentage was observed after 24 h for both Diptera flies with(66.67 ± 2.89)% of Bactrocera cucurbitae larvae killed by ethyl acetate extract of J. curcas bark.Conclusions: This paper compared the different J. curcas plant sections with respect to the effectiveness of the plant as a potential candidate for new pharmaceuticals. The larvicidal effect was also studied in order to demonstrate the dual purpose of the plant. 展开更多
关键词 jatropha curcas ANTIMICROBIAL PHYTOCHEMICAL LARVICIDAL
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Distribution and development strategy for Jatropha curcas L. in Yunnan Province, Southwest China 被引量:9
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作者 Li Kun Yang Wen-yun +3 位作者 Li Li Zhang Chun-hua Cui Yong-zhong Sun Yong-yu 《Forestry Studies in China》 CAS 2007年第2期120-126,共7页
Yunnan Province is the main distributing area ofJatropha curcas L. This plant is abundant in several drainage areas of the dry-hot, dry-warm and sub-humid valleys in the south subtropical area of Yunnan Province. The ... Yunnan Province is the main distributing area ofJatropha curcas L. This plant is abundant in several drainage areas of the dry-hot, dry-warm and sub-humid valleys in the south subtropical area of Yunnan Province. The seeds that were picked from trees blossoming between April and May and fructifying between September and October will have large seed yield and fine quality. For developing bio-diesel stock forest ofJ. curcas in areas with adaptive climate, seeding measures for afforestation should be taken and techniques on breeding, fast-growing, and high-yielding plantation cultivation are very important. 展开更多
关键词 Yunnan Province jatropha curcas L. geographical distribution resource status
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Floral display and breeding system of Jatropha curcas L. 被引量:6
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作者 Luo Chang-wei Li Kun +1 位作者 Chen You Sun Yong-yu 《Forestry Studies in China》 CAS 2007年第2期114-119,共6页
Plant flowering and breeding characteristics are important for us to understand the reproduction of plant populations. In this paper, we studied the reproduction characteristics of Jatropha curcas in Yuanjiang County ... Plant flowering and breeding characteristics are important for us to understand the reproduction of plant populations. In this paper, we studied the reproduction characteristics of Jatropha curcas in Yuanjiang County (23°36'1'4, 101°00'E), Yunnan Province. The plant produces flowers in dichasial inflorescences. Normally, the flowers are unisexual, and male and female flowers are produced in the same inflorescence. Only a few male flowers are produced in an inflorescence, and fruits are produced only through pollination between different flowers from the same or different plants. By the treatments of emasculation, bagging and artificial pollination in this experiment, there were few but same fruit set ratios when the inflorescences were emasculated, bagged, or bagged with net, except artificial pollination treatments, which showed that Jatropha curcas could produce fruit through apomixis but not wind pollination. When the inflorescences were unbagged, unemasculated and with free pollination treatments, or bagged, emasculated and with artificial cross-pollination treatments, or unbagged, emasculated and with free pollination treatments, there were many fruits produced. It showed that Jatropha curcas shows outcrossing, is self-compatible, and demanding for pollinators. Normally, the male flowers open first and a few flowers bloom in one day in a raceme. These flowers last a long time in bloom. However, a large number of female flowers open from the third to the fifth day, with some female flowers opening first in a few raceme. This shows a tendency to promote xenogamy and minimize geitonogamy. 展开更多
关键词 jatropha curcas floral display breeding system outcrossing index pollen-ovule ratio
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Cloning and characterization of a stearoyl-ACP desaturase gene from Jatropha curcas 被引量:4
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作者 罗通 马丹炜 +4 位作者 徐莺 邓骛远 肖猛 卿人韦 陈放 《Journal of Shanghai University(English Edition)》 CAS 2007年第2期182-188,共7页
Using degenerate primers and RT-PCR, RACE techniques, a 1491 bp cDNA segment of stearoyl-acyl carrier protein desaturase (SAD) is cloned from developing seeds of Jatropha curcas L. The segment contains a 1191 bp of ... Using degenerate primers and RT-PCR, RACE techniques, a 1491 bp cDNA segment of stearoyl-acyl carrier protein desaturase (SAD) is cloned from developing seeds of Jatropha curcas L. The segment contains a 1191 bp of complete open reading frame (ORF). Analysis in the BLAST on NCBI shows that Jatropha curcas SAD (JSAD) gene encodes a protein precursor composed of a signal peptide of 33 amino acids and a mature peptide of 363 amino acids. The homological analysis shows that JSAD has high level of homology both in nucleotide sequence and in amino acid sequence to other plants SADs. The nucleotide and peptide identity of JSAD to Ricinus communis SAD (RSAD) is up to 89% and 96.2% respectively. Molecular modeling of JSAD indicates that its three-dimensional structure strongly resembled the crystal structure of RSAD. 展开更多
关键词 jatropha curcas stearoyl-acyl carrier protein desaturase fatty acid gene cloning bioinformatics analysis
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The biomedical significance of the phytochemical, proximate and mineral compositions of the leaf, stem bark and root of Jatropha curcas 被引量:2
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作者 Atamgba Agbor Asuk Margaret Akpana Agiang +1 位作者 Kayode Dasofunjo Amonor James Willie 《Asian Pacific Journal of Tropical Biomedicine》 SCIE CAS 2015年第8期635-641,共7页
Objective: To analyse the phytochemical contents of leaf, stem bark and root of Jatropha curcas(J. curcas) in four solvent extracts and their proximate and mineral compositions. Methods: Standard analytical procedures... Objective: To analyse the phytochemical contents of leaf, stem bark and root of Jatropha curcas(J. curcas) in four solvent extracts and their proximate and mineral compositions. Methods: Standard analytical procedures were used for the determination of phytochemicals, proximate and mineral compositions of the leaf, stem bark and root extracts of J. curcas. Results: Results of the analysis showed the presence of polyphenols, flavonoids, alkaloids, cardiac glycosides, coumarins, saponins, terpenoids, steroids, triterpenoid saponins, carotenoids, phlobatannins and tannins in the leaf, stem bark and root of all the solvent extracts. Flavonoids were present in the highest amount in the ethyl acetate extracts of the leaf(7.35% ± 0.02%), stem bark(4.12% ± 0.01%) and root(3.35% ± 0.02%) followed by polyphenols in the methanol extracts of leaf(4.62% ± 0.02%), stem bark(2.77% ± 0.05%) and root(2.49% ± 0.02%). Poly-acetylated compounds were absent in all the solvent extracts of the leaf, stem bark and root. However, some anti-nutritional agents such as oxalates, phytates and cyanates were present in all the solvent extracts of the leaf, stem bark and root except the ethyl acetate. Phytates were high in the aqueous solvent of the leaf(6.12% ± 0.00%) but low in the stem bark(1.00% ± 0.05%) and root(0.89% ± 0.03%). Proximate composition showed appreciable amounts of total carbohydrate(36.33% ± 0.72%), crude protein(26.00% ± 0.47%) and reducing sugars(5.87% ± 0.14%) in the leaf, while crude fat was more in the stem bark(16.70% ± 0.30%). There was corresponding substantial energy in the leaf [(1 514.77 ± 20.87) kJ /100 g] and stem bark [(907.00 ± 8.52) kJ /100 g]. Moisture and ash contents of the leaf, stem bark and root were within acceptable limits for the use in drugs formulation. The mineral composition showed substantial amounts of important elements such as Fe, Ca, Na, Mg and Zn. Others were P, K and Se. Conclusions: The outcome of this study suggests that the leaf, stem bark and root of J. curcas have very good medicinal potentials, meet the standard requirements for drug formulation and serve as good sources of energy and nutrients except for the presence of some anti-nutritional elements predominant in the leaf. 展开更多
关键词 jatropha curcas Solvent extracts PHYTOCHEMICALS PROXIMATE COMPOSITION Mineral COMPOSITION
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<i>In</i><i>Silico</i>Mining of EST-SSRs in Jatropha curcas L. towards Assessing Genetic Polymorphism and Marker Development for Selection of High Oil Yielding Clones 被引量:2
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作者 Neeraj Jain Ganesh B. Patil +1 位作者 Poonam Bhargava Rajani S. Nadgauda 《American Journal of Plant Sciences》 2014年第11期1521-1541,共21页
In recent years, Jatropha curcas L. has gained popularity as a potential biodiesel plant. The varying oil content, reported between accessions belonging to different agroclimatic zones, has necessitated the assessment... In recent years, Jatropha curcas L. has gained popularity as a potential biodiesel plant. The varying oil content, reported between accessions belonging to different agroclimatic zones, has necessitated the assessment of the existing genetic variability to generate reliable molecular markers for selection of high oil yielding variety. EST derived SSR markers are more useful than genomic markers as they represent the transcriptome, thus, directly linked to functional genes. The present report describes the in silico mining of the microsatellites (SSRs) using J. curcas ESTs from various tissues viz. embryo, root, leaf and seed available in the public domain of NCBI. A total of 13,513 ESTs were downloaded. From these ESTs, 7552 unigenes were obtained and 395 SSRs were generated from 377 SSR-ESTs. These EST-SSRs can be used as potential microsatellite markers for diversity analysis, MAS etc. Since the Jatropha genes carrying SSRs have been identified in this study, thus, EST-SSRs directly linked to genes will be useful for developing trait linked markers. 展开更多
关键词 BIO-DIESEL jatropha curcas L. EST SSR MAS Diversity Marker MINING Polymorphism In Silico
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Root Anatomical Structure of <i>Jatropha curcas</i>Seedlings—A Short Report 被引量:1
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作者 Jun Abe 《American Journal of Plant Sciences》 2019年第3期491-495,共5页
Jatropha curcas has been expected as a biodiesel plant which can be grown in degraded lands. The structure of roots at the seedling stage, in particular cell wall modification in exodermis and endodermis, was microsco... Jatropha curcas has been expected as a biodiesel plant which can be grown in degraded lands. The structure of roots at the seedling stage, in particular cell wall modification in exodermis and endodermis, was microscopically observed. In addition, it was discussed if the first four peripheral roots that emerge from the base of the primary root (taproot) are lateral roots or adventitious roots. The primary root and the first-order lateral roots formed diarchy stele, in which two protoxylem poles present in primary xylem of root. Consequently, the first four peripheral roots cannot be lateral roots, but should be adventitious roots formed at the base of hypocotyl. In both the primary and first-order lateral roots, exodermis and endodermis formed highly lignified cell walls. Moreover, the exodermal and endodermal cell walls formed Casparian strips, which could be observed without special staining by fluorescent dye under ultraviolet microscopy. Such cell-wall modification in root exodermis and endodermis may play an important role for J. curcas under soil stresses in degraded lands. 展开更多
关键词 Adventitious ROOT Casparian Strip jatropha curcas ROOT Endo-dermis ROOT EXODERMIS
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