Objective:To investigate the effects of Jiangan Xiaozhi Formula(JGXZ)on oxidative stress and inflammatory factors in patients with non-alcoholic fatty liver disease(NAFLD).Methods:Between September 2022 and December 2...Objective:To investigate the effects of Jiangan Xiaozhi Formula(JGXZ)on oxidative stress and inflammatory factors in patients with non-alcoholic fatty liver disease(NAFLD).Methods:Between September 2022 and December 2023,our hospital admitted a total of 58 patients with NAFLD.These patients were split into two groups at random:one for experimentation and the other for control.There were 27 patients in the experimental group at the end,compared to 26 in the control group,reasonable exercise,weight management,lipid regulation,and oral polyene phosphatidylcholine capsules(PPC).The experimental group received JGXZ in addition to the above treatments for 12 consecutive weeks.Changes in Traditional Chinese Medicine(TCM)syndrome scores,blood lipids,liver function indicators,the levels of oxidative stress markers,such as malondialdehyde(MDA),glutathione peroxidase(GSH-Px),and superoxide dismutase(SOD),as well as serum inflammatory factors,such as interleukin-6(IL-6),interleukin-8(IL-8),and tumor necrosis factor-α(TNF-α),were measured both before and after treatment.Results:After treatment,both groups showed significant reductions in TCM syndrome scores(P<0.05)and improvements in blood lipids,liver function indicators,inflammatory factors,and oxidative stress markers(P<0.05).Improvements were noticeably better in the experimental group than in the control group.(P<0.05).Conclusion:JGXZ can significantly improve clinical symptoms,regulate blood lipids,and protect liver function in patients with NAFLD.Its mechanism may be related to inhibiting inflammatory responses and regulating the balance of the oxidation-antioxidation system.展开更多
Objective:To investigate the therapeutic effects of Jiangan Xiaozhi decoction on nonalcoholic steatohepatitis(NASH)mice model and its effects on the endoplasmic reticulum stress(ERS)signaling pathway.Methods:NASH mice...Objective:To investigate the therapeutic effects of Jiangan Xiaozhi decoction on nonalcoholic steatohepatitis(NASH)mice model and its effects on the endoplasmic reticulum stress(ERS)signaling pathway.Methods:NASH mice model was established by methionine-choline deficient(MCD)diet induction and intragastric administration of Jiangan Xiaozhi decoction.The therapeutic effects of the decoction on NASH mice model were evaluated by measuring the body weight,hepatic index,and blood lipids and analyzing liver function-related biochemical markers,liver hematoxylin and eosin staining,and liver oil red O staining after the administration of the decoction in the mice model.Next,the levels of glucose-regulated protein 78(GRP78),inositol-requiring enzyme 1(IRE1),and X-box binding protein 1(XBP1)in the liver tissue of the mice were measured using Western blotting to investigate the mechanism underlying the treatment of NASH mice model by Jiangan Xiaozhi decoction.Results:Jiangan Xiaozhi decoction improved the body weight and hepatic index;reduced the levels of total cholesterol,transglutaminase,alanine transaminase,and aspartate aminotransferase;and improved pathological changes caused by hepatocyte necrosis,inflammatory cell infiltration,ballooning,and fat accumulation in the liver tissue of NASH mice model.The Western blot results showed that Jiangan Xiaozhi decoction reduced the levels of GRP78,IRE1,and XBP1 proteins in the liver tissues of NASH mice model.Conclusion:Jiangan Xiaozhi decoction has therapeutic effects on NASH mice model.The mechanism of its action may be related to the regulation of level of GRP78,IRE1,and XBP1 proteins in liver tissue and improvement of ERS.展开更多
基金supported by National Natural Science Foundation of China,Youth Science Fund Project(82104820)Zhejiang Natural Science Foundation Project(LTGY23H270009)+1 种基金Applied Basic Research Project of Jiaxing Science and Technology Bureau(2023AY11035)Applied Basic Research Project of Jiaxing Science and Technology Bureau(2023AD11047).
文摘Objective:To investigate the effects of Jiangan Xiaozhi Formula(JGXZ)on oxidative stress and inflammatory factors in patients with non-alcoholic fatty liver disease(NAFLD).Methods:Between September 2022 and December 2023,our hospital admitted a total of 58 patients with NAFLD.These patients were split into two groups at random:one for experimentation and the other for control.There were 27 patients in the experimental group at the end,compared to 26 in the control group,reasonable exercise,weight management,lipid regulation,and oral polyene phosphatidylcholine capsules(PPC).The experimental group received JGXZ in addition to the above treatments for 12 consecutive weeks.Changes in Traditional Chinese Medicine(TCM)syndrome scores,blood lipids,liver function indicators,the levels of oxidative stress markers,such as malondialdehyde(MDA),glutathione peroxidase(GSH-Px),and superoxide dismutase(SOD),as well as serum inflammatory factors,such as interleukin-6(IL-6),interleukin-8(IL-8),and tumor necrosis factor-α(TNF-α),were measured both before and after treatment.Results:After treatment,both groups showed significant reductions in TCM syndrome scores(P<0.05)and improvements in blood lipids,liver function indicators,inflammatory factors,and oxidative stress markers(P<0.05).Improvements were noticeably better in the experimental group than in the control group.(P<0.05).Conclusion:JGXZ can significantly improve clinical symptoms,regulate blood lipids,and protect liver function in patients with NAFLD.Its mechanism may be related to inhibiting inflammatory responses and regulating the balance of the oxidation-antioxidation system.
文摘Objective:To investigate the therapeutic effects of Jiangan Xiaozhi decoction on nonalcoholic steatohepatitis(NASH)mice model and its effects on the endoplasmic reticulum stress(ERS)signaling pathway.Methods:NASH mice model was established by methionine-choline deficient(MCD)diet induction and intragastric administration of Jiangan Xiaozhi decoction.The therapeutic effects of the decoction on NASH mice model were evaluated by measuring the body weight,hepatic index,and blood lipids and analyzing liver function-related biochemical markers,liver hematoxylin and eosin staining,and liver oil red O staining after the administration of the decoction in the mice model.Next,the levels of glucose-regulated protein 78(GRP78),inositol-requiring enzyme 1(IRE1),and X-box binding protein 1(XBP1)in the liver tissue of the mice were measured using Western blotting to investigate the mechanism underlying the treatment of NASH mice model by Jiangan Xiaozhi decoction.Results:Jiangan Xiaozhi decoction improved the body weight and hepatic index;reduced the levels of total cholesterol,transglutaminase,alanine transaminase,and aspartate aminotransferase;and improved pathological changes caused by hepatocyte necrosis,inflammatory cell infiltration,ballooning,and fat accumulation in the liver tissue of NASH mice model.The Western blot results showed that Jiangan Xiaozhi decoction reduced the levels of GRP78,IRE1,and XBP1 proteins in the liver tissues of NASH mice model.Conclusion:Jiangan Xiaozhi decoction has therapeutic effects on NASH mice model.The mechanism of its action may be related to the regulation of level of GRP78,IRE1,and XBP1 proteins in liver tissue and improvement of ERS.
