Effect of Shengsui Jiangu capsule on TNF-α, TGF-β1, and empty bone lacuna in rats with alcoholic osteoporosis

TGF-Objective:To investigate the effect of postgraduate Shengsui Jiangu Capsule on tumor necrosis factors TNF-α,transforming growth factor TGF-β1 and empty bone lacuna in rats with alcoholic osteoporosis.Method:120 SD rats were randomly divided into 4 groups,30 rats in each group:blank group,model group,western medicine control group and traditional Chinese medicine intervention group.Liquor gavage was used to model the disease,except for the blank group.The western medicine control group was gavaged with a suspension of calcium carbonate and afad3,while the Chinese medicine intervention group was gavaged with a suspension of Shengsui Jiangu capsules combined with 0.9%saline solution injection.The values of TNF-αand TGF-β1 in femora were measured after 8,12,and 16 weeks of intervention.After dissecting out the femoral head using the HE(hematoxylin-eosin staining)staining method,the bone lacunae were observed under a light microscope,and the empty bone lacunae rate was calculated for intergroup comparison.Results:Compared with the blank group,the model group had a significantly higher percentage of TNF-αand empty bone lacunae,and a significantly lower percentage of TGF-β1(P<0.01).Compared with the model group,the rates of TNF-αand empty bone lacunae in the western medicine control group and the Chinese medicine intervention group were significantly decreased,and TGF-β1 was significantly increased(P<0.01).The traditional Chinese medicine intervention group was more effective than the western medicine control group(P<0.05).Conclusion:Shengsui Jiangu capsule attenuates the expression of TNF-α,enhances the expression of TGF-β1,decreases the rate of empty bone lacunae,and achieves a protective effect against alcoholic osteoporosis in rats with alcoholic osteoporosis.

Effect of Shengsui Jiangu Capsule on bone conversion in rats with alcoholic osteoporosis

Objective:To study the mechanism of Shengsui Jiangu Capsule promoting vitamin D_(3)regulating bone turnover in rats with alcoholic osteoporosis.Methods:120 SD rats were randomly divided into four groups:model group(alcoholic osteoporosis model group),blank control group,western medicine control group(calcium carbonate tablet and affa D_(3)control group),Chinese medicine experimental group(Shengsui Jiangu capsule experimental group),30 rats in each group.After 8,12 and 16 weeks,the serum levels of 25 hydroxyvitamin D_(3)(25(OH)D_(3)),1,25dihydroxyvitamin D_(3)(1,25(OH)_(2)D_(3)),cross-linked carboxy-terminal peptide of type 1 collagen(β-CTX)and N-terminal propeptide of type 1 collagen(P1NP)in rats and the density of the femur of rats were detected and compared.The correlation between vitamin D_(3)markers(25(OH)D_(3)and 1,25(OH)_(2)D_(3))and bone turnover indices(P1NP andβ-CTX)and bone mineral density was analyzed.Results:In the experimental group,bone mineral density(BMD)was significantly increased(P<0.01),bone repair and mineral stability and balance were maintained during bone turnover,the level of bone formation marker P1NP was significantly increased(P<0.01),and the level of bone resorption markerβ-CTX was significantly decreased(P<0.01).Serum levels of 25(OH)D_(3)and 1,25(OH)_(2)D_(3)were increased(P<0.01),and significantly higher than those in western medicine control group(P<0.05).Conclusion:Shengsui Jiangu capsule can effectively improve bone density in alcoholic osteoporosis(AOP)rats,and its mechanism may be related to promoting bone calcium absorption and regulating bone conversion.