BACKGROUND Ulcerative colitis(UC)is a refractory intestinal disease with alternating onset and remission and a long disease course,which seriously affects the health and quality of life of patients.The goal of treatme...BACKGROUND Ulcerative colitis(UC)is a refractory intestinal disease with alternating onset and remission and a long disease course,which seriously affects the health and quality of life of patients.The goal of treatment is to control clinical symptoms,induce and maintain remission,promote mucosal healing,and reduce recurrence.Clinical trials have shown unsatisfactory clinical response rates.As a supplementary alternative medicine,traditional Chinese medicine has a rich history and has shown good results in the treatment of UC.Because of the quality of herbal medicine and other factors,the curative effect of traditional Chinese medicine is not stable enough.The mechanism underlying the effect of Jianpi Qingchang Huashi Recipe(JPQCHSR)on inducing UC mucosal healing is not clear.AIM To investigate the potential mechanism of JPQCHSR for the treatment of UC based on network pharmacology and molecular docking.METHODS Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform was used to extract the active components and action targets of JPQCHSR,and the target names were standardized and corrected through UniProt database.The related targets of UC were obtained through GeneCards database,and the intersection targets of drugs and diseases were screened by jvenn online analysis tool.The visual regulatory network of"Traditional Chinese medicine-active components-target-disease"was constructed using Cytoscape software,the protein interaction network was constructed using STRING database,and enrichment analysis of gene ontology and Kyoto Encyclopedia of Genes and Genomes pathways was conducted through R software.At last,the active components were docked with the core target through SYBYL-X 2.1.1 software.RESULTS Through database analysis,a total of 181 active components,302 targets and 205 therapeutic targets were obtained for JPQCHSR.The key compounds include quercetin,luteolin,kaempferol,etc.The core targets involved STAT3,AKT1,TP53,MAPK1,MAPK3,JUN,TNF,etc.A total of 2861 items were obtained by GO enrichment analysis,and 171 items were obtained by KEGG(Kyoto Encyclopedia of Genes and Genomes)pathway enrichment analysis.The results of molecular docking showed that the key active components in JPQCHSR had certain affinity with the core target.CONCLUSION The treatment of UC with JPQCHSR is a complex process of multi-component,multi-target and multi-pathway regulation.The mechanism of this Recipe in the treatment of UC can be predicted through network pharmacology and molecular docking,so as to provide theoretical reference for it to better play its therapeutic role.展开更多
目的:观察健脾化湿颗粒对腹泻型肠易激综合征(diarrhea-predominant irritable bowel syndrome,D-IBS)模型大鼠结肠运动功能的影响,并从中枢c-fos角度探讨其作用机制.方法:采用番泻叶灌胃结合束缚应激法建立D-I B S大鼠模型,应用健脾化...目的:观察健脾化湿颗粒对腹泻型肠易激综合征(diarrhea-predominant irritable bowel syndrome,D-IBS)模型大鼠结肠运动功能的影响,并从中枢c-fos角度探讨其作用机制.方法:采用番泻叶灌胃结合束缚应激法建立D-I B S大鼠模型,应用健脾化湿颗粒进行干预,采用Maclab/4e四导生理记录仪描记大鼠结肠运动曲线,计算结肠运动指数,并用免疫组织化学法检测脊髓腰膨大、前额叶皮质、海马、下丘脑中c-fos的表达,ELISA法检测脊髓腰膨大和海马中c-fos的含量.结果:与正常组相比,D-IBS模型组大鼠结肠运动指数(939.01±91.96 vs 574.78±53.33)明显增加(P<0.01).中枢c-fos的表达(0.25±0.03 v s 0.08±0.01、0.15±0.02 v s 0.06±0.01、0.23±0.02 vs 0.08±0.02、0.16±0.02vs 0.07±0.01)明显升高(P<0.01).c-fos含量(19.68±1.17 v s 11.10±2.59、12.32±1.22vs 5.38±0.88)明显增加(P<0.01).与模型组相比,健脾化湿颗粒中、高剂量组大鼠结肠运动指数(731.91±67.34、695.20±80.58 vs939.01±91.96)明显降低(P<0.01).中枢c-fos的表达(0.14±0.02、0.10±0.01 vs 0.25±0.03;0.11±0.01、0.09±0.02 vs 0.15±0.02;0.16±0.01、0.12±0.01 vs 0.23±0.02;0.12±0.01、0.09±0.01 vs 0.16±0.02)明显降低(P<0.01).c-f o s含量(16.02±1.58、15.62±1.64 vs 19.68±1.17;8.53±2.05、7.28±1.54vs 12.32±1.22)明显降低(P<0.01).结论:健脾化湿颗粒改善D-IBS模型大鼠结肠运动功能的作用机制,可能与其调节中枢c-fos的表达有关.展开更多
基金Shaanxi Province Natural Science Basic Research Program-General Project,No.2019JM-580 and No.2021SF-314Project of Shaanxi Administration of Traditional Chinese Medicine,No.2019-ZZ-JC010Key Clinical Specialty Construction Project supported by Hongkou District Health Committee,No.HKZK2020A01.
文摘BACKGROUND Ulcerative colitis(UC)is a refractory intestinal disease with alternating onset and remission and a long disease course,which seriously affects the health and quality of life of patients.The goal of treatment is to control clinical symptoms,induce and maintain remission,promote mucosal healing,and reduce recurrence.Clinical trials have shown unsatisfactory clinical response rates.As a supplementary alternative medicine,traditional Chinese medicine has a rich history and has shown good results in the treatment of UC.Because of the quality of herbal medicine and other factors,the curative effect of traditional Chinese medicine is not stable enough.The mechanism underlying the effect of Jianpi Qingchang Huashi Recipe(JPQCHSR)on inducing UC mucosal healing is not clear.AIM To investigate the potential mechanism of JPQCHSR for the treatment of UC based on network pharmacology and molecular docking.METHODS Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform was used to extract the active components and action targets of JPQCHSR,and the target names were standardized and corrected through UniProt database.The related targets of UC were obtained through GeneCards database,and the intersection targets of drugs and diseases were screened by jvenn online analysis tool.The visual regulatory network of"Traditional Chinese medicine-active components-target-disease"was constructed using Cytoscape software,the protein interaction network was constructed using STRING database,and enrichment analysis of gene ontology and Kyoto Encyclopedia of Genes and Genomes pathways was conducted through R software.At last,the active components were docked with the core target through SYBYL-X 2.1.1 software.RESULTS Through database analysis,a total of 181 active components,302 targets and 205 therapeutic targets were obtained for JPQCHSR.The key compounds include quercetin,luteolin,kaempferol,etc.The core targets involved STAT3,AKT1,TP53,MAPK1,MAPK3,JUN,TNF,etc.A total of 2861 items were obtained by GO enrichment analysis,and 171 items were obtained by KEGG(Kyoto Encyclopedia of Genes and Genomes)pathway enrichment analysis.The results of molecular docking showed that the key active components in JPQCHSR had certain affinity with the core target.CONCLUSION The treatment of UC with JPQCHSR is a complex process of multi-component,multi-target and multi-pathway regulation.The mechanism of this Recipe in the treatment of UC can be predicted through network pharmacology and molecular docking,so as to provide theoretical reference for it to better play its therapeutic role.
文摘目的:观察健脾化湿颗粒对腹泻型肠易激综合征(diarrhea-predominant irritable bowel syndrome,D-IBS)模型大鼠结肠运动功能的影响,并从中枢c-fos角度探讨其作用机制.方法:采用番泻叶灌胃结合束缚应激法建立D-I B S大鼠模型,应用健脾化湿颗粒进行干预,采用Maclab/4e四导生理记录仪描记大鼠结肠运动曲线,计算结肠运动指数,并用免疫组织化学法检测脊髓腰膨大、前额叶皮质、海马、下丘脑中c-fos的表达,ELISA法检测脊髓腰膨大和海马中c-fos的含量.结果:与正常组相比,D-IBS模型组大鼠结肠运动指数(939.01±91.96 vs 574.78±53.33)明显增加(P<0.01).中枢c-fos的表达(0.25±0.03 v s 0.08±0.01、0.15±0.02 v s 0.06±0.01、0.23±0.02 vs 0.08±0.02、0.16±0.02vs 0.07±0.01)明显升高(P<0.01).c-fos含量(19.68±1.17 v s 11.10±2.59、12.32±1.22vs 5.38±0.88)明显增加(P<0.01).与模型组相比,健脾化湿颗粒中、高剂量组大鼠结肠运动指数(731.91±67.34、695.20±80.58 vs939.01±91.96)明显降低(P<0.01).中枢c-fos的表达(0.14±0.02、0.10±0.01 vs 0.25±0.03;0.11±0.01、0.09±0.02 vs 0.15±0.02;0.16±0.01、0.12±0.01 vs 0.23±0.02;0.12±0.01、0.09±0.01 vs 0.16±0.02)明显降低(P<0.01).c-f o s含量(16.02±1.58、15.62±1.64 vs 19.68±1.17;8.53±2.05、7.28±1.54vs 12.32±1.22)明显降低(P<0.01).结论:健脾化湿颗粒改善D-IBS模型大鼠结肠运动功能的作用机制,可能与其调节中枢c-fos的表达有关.
