Compositional characteristics of sediment from Jiaozhou Bay in North China and the implication to the provenance

Rare earth elements(REEs)can be used to trace source materials and identify their provenances,because of significant conservation and immobility during chemical alteration processes after erosion of materials from the provenance.This study focused on the temporal variation of REEs for columnar sediments from the mouth of Jiaozhou Bay in North China to understand the potential controls for the geochemical variations of sediments.Through extraction experiments,we identified that the residual fraction is the main host for REEs compared with other fractions(i.e.,exchangeable and carbonate fraction,easily reducible oxides fraction,reducible oxides fraction,magnetite fraction).REE ratios(e.g.,La_(N)/Sm_(N)and La_(N)/Yb_(N);N:normalized by chondrite)lack correlations with grain size or the chemical index of alteration(CIA),which is correlated with major elements.All these indicate that these REE variations reflect the varying contribution of source materials from different provenances instead of grain size or chemical weathering effects.REE ratios(e.g.,La_(N)/Sm_(N)and La_(N)/Yb_(N))remain relatively constant until the depth of roughly 40 cm(equivalent to the year 1995),and show obvious changes beyond this depth.Compared REE characteristics of Jiaozhou Bay with those of neighboring rivers and bedrocks,the relative contributions of Dagu River-Jiaolai River,and Licun River may have been increased during the sedimentary processes,which could be caused by the construction of reservoir and related change of aquaculture(e.g.,rapid accumulation of organic materials).

Establishing environmental background values of selected trace elements and environmental quality assessment of fi negrained sediments in the Jiaozhou Bay catchment,China

With the development of economy,the impact of human activities on ecological environment is increasing,and environmental protection work is important.Trace elements(Co,Ni,Cu,Pb,Zn,Cd,Cr,and Sc)in surface fi ne-grained sediment samples from the Jiaozhou Bay catchment were selected to evaluate their 2015 environmental background values and the environmental quality.Using statistical analysis,the environmental background values(ranges)of Sc,Co,Ni,Cu,Pb,Zn,Cd,and Cr were calculated,being 13.1(10.8-15.4),12.4(8.6-16.2),32.0(22.9-41.2),29.6(13.5-64.9),24.1(13.0-44.6),77.6(38.5-156.5),0.07(0.02-0.20),and 82.5(66.5-104.0)mg/kg,respectively.The enrichment factor,contamination factor,and pollution load index were used to evaluate the pollution status of the Jiaozhou Bay catchment.The environmental background values of most elements are higher than those of the upper continental crust and lower than those of global shale.At present,the pollution in the eastern Jiaozhou Bay is much higher than that in the western part.The results shall be helpful for future management for trace element pollution monitoring in the Jiaozhou Bay catchment.

The sources and composition of organic matter in sediments of the Jiaozhou Bay：implications for environmental changes on a centennial time scale

The Jiaozhou Bay is characterized by heavy eutrophication that is associated with intensive anthropogenic activities. Four core sediments from the Jiaozhou Bay are analyzed using bulk technologies, including sedimentary total organic carbon(TOC), total nitrogen(TN), the stable carbon(δ^(13)C) and nitrogen(δ^(15) N) isotopic composition to obtain the comprehensive understanding of the source and composition of sedimentary organic matter and further shed light on the environmental changes of the Jiaozhou Bay on a centennial time scale.Results suggest that the TOC and TN concentrations increase in the upper core, having indicated a probable eutrophication process since the 1920 s in the inner bay and the 2000 s in the bay mouth. The TOC and TN concentrations outside the bay have also changed since 1916 owing to the variation of terrigenous input.Considering TOC/TN ratio, δ^(13) C and δ^(15) N, it can be concluded there is a mixture of terrigenous and marine organic matter sources in the study area. A simple two end-member(terrigenous and marine) mixing model usingδ^(13) C indicats that 45%–79% of TOC in the Jiaozhou Bay is from the marine source. The environmental changes of the Jiaozhou Bay are recorded by geochemical proxies, which are influenced by the intensive anthropogenic activities(e.g., extensive use of fertilizers, and discharge of sewage) and climate changes(e.g., rainfall).

Winter Phytoplankton Assemblages of Coastal Yellow Sea Connected to Jiaozhou Bay,China

The daily species variation of phytoplankton assemblage of coastal Yellow Sea connected to Jiaozhou Bay in the sea-water around the Xiaoqingdao Island was studied for the three months of winter, 2003. A total of 79 taxa from four phyla, Bacil-lariophyta (52 species), Pyrrophyta (25 species), Chrysophyta (1 species), and Cyanophyta (1species) were determined. In general, the most important groups were Bacillariophyta and Pyrrophyta on cell abundance and species richness. In January, the dominant species was mainly composed of cosmopolitan species such as Skeletonema costatum, then it changed to cosmopolitan species Tha-lassiosira nordenskiodii in February, and finally, it recovered to Skeletonema costatum again in March. Cell abundance ranged from 1.02 to 130.71×103 cells L-1with a single peak on 6th Feb., and the average abundance was 28.11 ± 26.01×103 cells L-1 (n = 90) during the winter time. The trends of Shannon-Weiner diversity index and Pielou’s evenness index were contrary to cell abundance, whereas the species diversity and evenness were the lowest in February. Temperature and salinity were closely correlated to phytoplankton species composition and cell abundance. According to the variations of temperature and salinity, species sequence was obvious at the studied site. This implies that the phytoplankton community of inner Jiaozhou Bay would be influenced heavily by the coastal Yel-low Sea phytoplankton assemblages.

The observations of seabed sediment erosion and resuspension processes in the Jiaozhou Bay in China

In estuarine and coastal areas, the seabed is in a constant process of dynamic change under marine conditions.Seabed sediment erosion and resuspension are important processes that safely control the geological environment. Field tripod observations conducted in the Jiaozhou Bay in China are reported, to investigate the effects of hydrodynamic conditions on the erosion and resuspension processes of the seabed. The observational results show that the maximum shear stress created by tidal currents can reach 0.35 N/m^2, which is higher than the wave-induced shear stress during fair weather conditions. A seabed erosion frequently occurs during the flood tide, whereas a seabed deposition occurs during ebb tide. Waves can produce a bottom shear stress approximately equivalent to that induced by currents when the local wind reaches Force 4 with a speed of 5 m/s.When the wind reaches 7 m/s and the significant wave height reaches 26 cm, waves play a more significant role than currents in the dynamic processes of the seabed sediment resuspension and lead to a high value of turbidity that is approximately two to eight times higher than that in fair weather. These analyses clearly illustrate that periodic current-induced sediment erosion and resuspension are dominant in fair weather, whereas episodic high waves are responsible for significant sediment resuspension. Additional work is needed to establish a more thorough understanding of the mechanisms of sediment dynamics in the Jiaozhou Bay.

Steady increase in water clarity in Jiaozhou Bay in the Yellow Sea from 2000 to 2018:Observations from MODIS

The Moderate Resolution Imaging Spectroradiometer(MODIS)surface reflectance data were used to analyze the temporal and spatial distribution characteristics of water clarity(Z_(sd))in the Jiaozhou Bay,Qingdao,China,in the Yellow Sea from 2000 to 2018.Z_(sd)retrieval models were regionally optimized using in-situ data with coincident MODIS images,and then were used to retrieve the Z_(sd) products in Jiaozhou Bay from 2000-2018.The analysis of the Z_(sd) results suggests that the spatial distribution of relative Z_(sd) spatial characteristics in Jiaozhou Bay was stable,being higher Z_(sd) in the southeast and a lower Z_(sd) in the northwest.The annual mean Z_(sd) in Jiaozhou Bay showed a significant upward trend,with an annual increase of approximately 0.02 m.Water depth and wind speed were important factors affecting the spatial distribution and annual variation of Z_(sd) in Jiaozhou Bay,respectively.

Seasonal dynamics of meiofaunal distribution in the Dagu River Estuary, Jiaozhou Bay, China

Sediment samples were collected in the intertidal zone of the Dagu River Estuary, Jiaozhou Bay, China in April,July and October 2010 and February 2011 for examining seasonal dynamics of meiofaunal distribution and their relationship with environmental variables. A total of ten meiofaunal taxa were identified, including free-living marine nematodes, benthic copepods, polychaetes, oligochaetes, bivalves, ostracods, cnidarians, turbellarians,tardigrades and other animals. Free-living marine nematodes were the most dominant group in both abundance and biomass. The abundances of marine nematodes were higher in winter and spring than those in summer and autumn. Most of the meiofauna distributed in the 0–2 cm sediment layer. The abundance of meiofauna in hightidal zone was lower than those in low-tidal and mid-tidal zones. Results of correlation analysis showed that Chlorophyll a was the most important factor to influence the seasonal dynamics of the abundance, biomass of meiofauna and abundances of nematodes and copepods. CLUSTER analysis divided the meiofaunal assemblages into three groups and BIOENV results indicated that salinity, concentration of organic matter, sediment sorting coefficient and sediment median diameter were the main environmental factors influencing the meiofaunal assemblages.

Diversity and seasonal variation of marine phytoplankton in Jiaozhou Bay, China revealed by morphological observation and metabarcoding

Phytoplankton are central components of marine environments,and are major players in the production and respiration budgeting.However,their diversity and distribution patterns are still poorly understood due largely to their small sizes and inconspicuous morphology that have been determined via the application of traditional morphology methods over the past two decades.To better understand the composition and diversity of phytoplankton in Jiaozhou Bay,China,seasonal sampling was carried out in 2019 and samples were analyzed with morphological observations and high-throughput sequencing,from which obvious seasonal variations in phytoplankton composition and proportional abundances were uncovered.Metabarcoding revealed far more diversity and species richness of phytoplankton than morphological observations,especially with respect to dinofl agellates.Diatoms were the most dominant phytoplankton group throughout the year,of which Thalassionema and Skeletonema were co-dominant in the bay.Parasitic dinofl agellates(e.g.Amoebophrya),which is often overlooked in the morphological observations,were in dominance and high diversity in the metabarcoding dataset,thus more attention should be paid to exploring the potential role of parasitic dinofl agellates.Temperature,chlorophyll a,and nutrient levels were the main infl uential factors on the distribution of phytoplankton.This study provided a comprehensive morphological and molecular description of phytoplankton and clearly demonstrated the importance of molecular technology in exploring phytoplankton communities.More-widespread use of molecular technology will facilitate deeper understanding of the ecological importance of the diff erent species.

Annual Occurrence of Calanus sinicus Carcasses in the Jiaozhou Bay and the Adjacent Waters

The dead proportion of a calanoid copepod Calanus sinicus population was investigated monthly with neutral red staining method at 12 stations in the Jiaozhou Bay and the adjacent waters from December 2008 to November 2009. C. sinicus could be observed through the whole year, with an evident numerical peak from February to April and an extremely low abundance in summer. Annual variation of the dead proportion differed greatly between adult females and copepodites. Dead females were observed only in the southern part and outside the bay sporadically in five months, when the egg production was the most active. Dead proportion of females was usually less than 5%. Dead copepodites could be observed in all but three months in summer in the whole study area, and the proportion varied between 3.8% – 18.2%. Death percentage of copepodites increased significantly in the northern part in January and June, when water temperature exceeded it's favorable limits;however, the fluctuated salinity had no significant effect on the survival of both females and copepodites. Thus the dead proportion of C. sinicus was relatively low in the Jiaozhou Bay, while difference could be observed between females and copepodites. Dead copepodites were common in all samples, but dead females were presented mainly as ‘reproduction cost' during generation alternation.

Ecological environment response of benthic foraminifera to heavy metals and human engineering: A case study from Jiaozhou Bay, China

The estuary and coastal zone are the key areas for socio-economic development,and they are also the important channels for pollutants transported to the sea.The construction of the Jiaozhou Bay Bridge changed the hydrodynamic condition of the bay,which made the self-purification capacity of the bay weakened and the pollution in the estuary and adjacent coastal zone become more serious.In this study,55 surface sediment samples were collected from the three seriously polluted estuaries and the adjacent coastal zone of Jiaozhou Bay to comprehensively study how the benthic foraminifera response to heavy metal pollution and human engineering,and to assess the ecological risks of the bay.A total of 80 species,belonging to 42 genera,were identified in this study.The results showed that Cu,Pb,Cr,Hg,Zn,and As had low to median ecological risks in the study area which would definitely affect the ecological system.The construction of the Jiaozhou Bay Bridge has resulted in pollutants accumulated at the river mouth of Loushan River,which has adverse effects on the survival and growth of benthic foraminifera.The lowest population density and diversity as well as the highest FAI(Foraminiferal Abnormality Index)and FMI(Foraminiferal Monitoring Index)occurred at Loushan River Estuary which indicated that the ecological environment of the northeastern part of Jiaozhou Bay(Loushan River Estuary)had been seriously damaged.Licun River and Haipo River estuaries and the adjacent coastal zone were slightly polluted and had low ecological risk.As a consequence,it suggested that the supervision of industrial and domestic waste discharge and the protection of the ecological environment in northeast Jiaozhou Bay should be paid more attention.

SIMPLE SIMULATION OF THE ANNUAL VARIATION OF THE SPECIFIC PHOTOSYNTHESIS RATE IN JIAOZHOU BAY

A simple diagnostic simulation of the annual cycling of the surface specific photosynthesis rate (SPR) in Jiaozhou Bay is described in this paper. Light intensity, temperature and nutrients (nitrate + ammonia, phosphate) were considered as main factors controlling photosynthesis of phytoplankton and were introduced into the model by different function equations. The simulated variation of specific photosynthesis rate coincided with the measured data. Analysis of the effect of every factor on photosynthesis indicated that the variation of photosynthesis rate was controlled by all these three factors, while temperature showed good correlation with SPR as measurement showed. This diagnostic simulation yielded the values of some parameter relating with the photosynthesis in Jiaozhou Bay.

Spatial variations of bacterial community composition in sediments of the Jiaozhou Bay,China

Spatial variations of sediment microbes pose a great challenge for the estimation of anthropogenic influence on biogeochemical processes,yet remain very unclear in coastal ecosystems.Surface sediments in 9 stations from the eutrophic Jiaozhou Bay,China,were sampled,DNA was extracted within the sediments,and the 16 S rDNA was sequenced with the Illumina Hiseq sequencing.Results reveal considerable heterogeneity of sediment bacteria in the Jiaozhou Bay,of which Proteobacteria and Bacteroidetes accounted for over 75%.Bacterial alpha-diversity indices decreased generally from the outside to the inner part of the bay and from the offshore to the nearshore area.Bacterial community structures of S3,S4,S7,and S8 clustered,those of S5,S13,and S14 grouped together,while those of S6 and S10 were distinct from each other and from those of the other stations.Major class Gammaproteobacteria were more abundant at the stations with mesoeutrophic to eutrophic levels(S4,S5,S8,and S10)and less abundant at oligotrophic stations(S6,S13,and S14),while Deltaproteobacteria had an opposite distribution pattern.Overall,bacterial community composition transitioned from being Xanthomonadales-dominant at S4 and S8 to being unidentifedGammaproteobacteria-dominant at S5,S6,S13,and S14,while in other stations there were comparable orders.The biogeochemical processes correspondingly changed from being nitrogen cycling-dominant at S4 and S8 to being sulfur cycling-dominant at S5,S6,S13,and S14.The bacterial distribution patterns were especially affected by the factors(dissolved organic phosphorus,DOP)in the overlying seawater due to the habitat status of P-insu fficiency in the bay.Both orders Xanthomonadales and Alteromonadales could serve as bioindicators of anthropogenic pollution to different pollution types.At last,divergent distribution patterns of individual bacterial populations in the bay were revealed,the influential environmental gradients were clarified,and the uncertainty of microbes was reduced,helping to predict environmental functions in coastal areas.

Abundance and characteristics of microplastics in shellfi sh from Jiaozhou Bay,China

As an emerging pollutant,the presence of microplastics in marine organisms has been concerned increasingly.Shellfi sh,which are both economically and ecologically important,are of particular concern.In this study,we investigated the microplastic pollution in wild and farmed oysters(Crassostrea gigas)and clams(Ruditapes philippinarum)in the Jiaozhou Bay,China,for the fi rst time.We found the microplastic pollution in shellfi sh in Jiaozhou Bay was at a moderate level.The abundance of microplastics in shellfi sh ranged from 0.16 to 12.09 items/g(wet weight,ww)or 1 to 9 items/ind.The average abundance of the ingested microplastics was 1.21 items/g(or 2.17 items/ind.)in all shellfi sh,1.51 items/g(or 2.00 items/ind.)in clams and 0.92 items/g(or 2.34 items/ind.)in oysters.The abundance of microplastics in clams was signifi cantly higher than that in oysters.Most microplastics(92.97%)were fi bers,followed by fragments.The predominant color of the microplastics was black(42.97%),followed by blue,transparent,and red.Cellophane and polyethylene terephthalate(PET)dominated the microplastic composition.According to shellfi sh consumption,it can be inferred that the average microplastic consumption through Chinese diet is 1.27×10^(3)items per capita per year.

Total Maximum Allocated Loads on Stoichiometry of Nitrogen and Identification of Critical Form in Jiaozhou Bay, China

Total pollutant load control management for total dissolved nitrogen(TDN) is an urgent task required to gain a good water quality status in Jiaozhou Bay(JZB), China. In this paper, the stoichiometry of multiform TDN on land-ocean interactions associated with marine biogeochemical reaction(LOIMBR) was studied by modeling the load-response relationship based on a three-dimensional water quality model of nitrogen in JZB. The results showed that the stoichiometry on LOIMBR of dissolved organic nitrogen(DON), NO3-N and NH4-N was 3:1:1, with one-third of the contribution on the concentration of dissolved inorganic nitrogen(DIN) in JZB for the land-based DON loads to DIN loads. Based on the stoichiometric relationship of nitrogen forms, the total maximum allocated load(TMAL) of equivalent TDN(ETDN) was approximately 5300 t a^-1 in JZB, equivalent to the TMAL of 5700, 5800 and 15600 t a^-1 for NH4-N, NO3-N and DON, respectively. According to the loads of ETDN, there were four outfalls overloaded in JZB in 2015, which lie in the head of the bay. In the four overloaded outfalls, besides NO3-N, NH4-N was the critical nitrogen control form for Moshui River, while DON for Dagu River and Haibo River. The results of numerical experiments further showed that JZB will achieve good water quality after 7 years by implementation of the 'different emission reduction' based on TMAL of ETDN, which is significantly better than 'equal percent removal'.

Sabatieria sinica sp.nov.(Comesomatidae,Nematoda) from Jiaozhou Bay,China

A new free-living marine nematode species of the genus Sabatieria was documented from the sediment of the Jiaozhou Bay,Qingdao,China,and it has been named Sabatieria sinica sp.nov.It is characterized by homogeneous cuticle without lateral differentiation,relatively short cephalic setae,spiral amphideal fovea with 2–2.25 turns;thick spicules with a mid constriction or joint,gubernaculum with a long straight dorso-caudal apophyses,and 6–7 prominent papillar precloacal supplements.This new species could be distinguished from other congener species by the combination of its characteristics:body size,length of cephalic setae,number of amphid turns,structures of spicules and gubernaculum,and type and number of precloacal supplements.An update dichotomous key is provided for nine species of pulchra group of Sabatieria including the new species.

Carbon biomass,carbon-to-chlorophyll a ratio and the growth rate of phytoplankton in Jiaozhou Bay,China

Carbon biomass,carbon-to-chlorophyll a ratio(C꞉Chl a),and the growth rate of phytoplankton cells were studied during four seasonal cruises in 2017 and 2018 in Jiaozhou Bay,China.Water samples were collected from 12 stations,and phytoplankton carbon biomass(phyto-C)was estimated from microscope-measured cell volumes.The phyto-C ranged from 5.05 to 78.52μg C/L in the bay,and it constituted a mean of 38.16% of the total particulate organic carbon in the bay.High phyto-C values appeared mostly in the northern or northeastern bay.Diatom carbon was predominant during all four cruises.Dinoflagellate carbon contributed much less(<30%)to the total phyto-C,and high values appeared often in the outer bay.The C꞉Chl a of phytoplankton cells varied from 11.50 to 61.45(mean 31.66),and high values appeared in the outer bay during all four seasons.The phyto-C was also used to calculate the intrinsic growth rates of phytoplankton cells in the bay,and phytoplankton growth rates ranged from 0.56 to 1.96/d;the rate was highest in summer(mean 1.79/d),followed by that in fall(mean 1.24/d)and spring(mean 1.17/d),and the rate was lowest in winter(mean 0.77/d).Temperature and silicate concentration were found to be the determining factors of phytoplankton growth rates in the bay.To our knowledge,this study is the first report on phytoplankton carbon biomass and C꞉Chl a based on water samples in Jiaozhou Bay,and it will provide useful information for studies on carbon-based food web calculations and carbon-based ecosystem models in the bay.

Environmental changes in Jiaozhou Bay of northern China during the past 90 years using metals and biogenic elements in sediments

Metals and biogenic elements were analyzed from surface sediments and a 100 cm core collected from Jiaozhou Bay in July 2009, to determine how the environment has changed over the past 90 years due to increasing anthropogenic influences in this region. High concentrations of biogenic silica(BSi) represented the dominance of diatoms in the bay. Most metals were lower than the marine sediment quality guidelines; however, Hg, Zn,and Mn were at polluted levels. The vertical profiles of biogenic elements and metals in the sediment core suggest that the most significant environmental changes occurred since the 1990 s, and three stages could be defined:(1) before 1950, characterized by low concentrations of biogenic elements and metals;(2) between 1950 and 1990, displaying an obvious increase of Hg and a slow increase of biogenic elements;(3) after 1990, reflected by a significant increase of total organic carbon(TOC), total nitrogen(TN), total phosphate(TP),and most metals, but a decrease of BSi. Correlation and principal component analyses indicated that most metals originated from lithogenic sources, industrial and domestic discharges as well as maricultural activities.

Evidence for coupled iron and nitrate reduction in the surface waters of Jiaozhou Bay

Iron and nitrate(NO_(3)^(-))are dominant physiologically required nutrients for phytoplankton growth,and iron may also play a key role in the marine nitrogen cycle.In this study,we investigated the temporal and spatial distributions of dissolved iron(DFe)and Fe(Ⅱ)in the surface waters of Jiaozhou Bay(JZB)from April 2 to July 26,2017.High concentrations of DFe and Fe(Ⅱ)predominantly occurred in nearshore and estuarine stations and concentrations were generally higher in April and May.The highest DFe concentration was observed along the coast of Hongdao(51.55 nmol/L)in May,while the lowest concentration was observed in the western coastal region(2.88 nmol/L)in April.The highest and lowest Fe(Ⅱ)concentrations were observed in the Licun estuary(22.42 nmol/L)and outer bay(0.50 nmol/L)in May,respectively.We calculated the proportions of nitrate,nitrite,and ammonium in dissolved inorganic nitrogen(DIN)as well as the ratio of Fe(Ⅱ)to DFe in all four months.The mean Fe(Ⅱ)/DFe ratio was 0.48 in April,0.43 in May,0.69 in June,and 0.32 in July.The mean ratio of NO_(3)^(-)to DIN was 0.78 in April,0.54 in May,0.20 in June,and 0.62 in July.NO_(3)^(-)/DIN continuously decreased in the first three months,while Fe(Ⅱ)/DFe remained high,which suggests that the reduction of iron and nitrate occurred simultaneously in the surface waters of JZB.

A mass balanced model of trophic structure and energy flows of a semi-closed marine ecosystem

The marine ecosystem of the Jiaozhou Bay has degraded significantly in fisheries productivity and its ecological roles as spawning and nursery ground for many species of commercial importance has been declining in recent years. A mass-balanced trophic model was developed using Ecopath with Ecosim to evaluate the trophic structure of the Jiaozhou Bay for improving ecosystem management. The model were parameterized based on the fisheries survey data in the Jiaozhou Bay in 2011, including 23 species groups and one detritus group according to their ecological roles. The trophic levels of these ecological groups ranged from 1(primary producers and detritus) to4.3(large demersal fishes). The estimated total system throughput was 12 917.10 t/(km^2·a), with 74.59% and25.41% contribution of the total energy flows from phytoplankton and detritus, respectively. Network analyses showed that the overall transfer efficiency of the ecosystem was 14.4%, and the mean transfer efficiency was 14.5%for grazing food chain and 13.9% for detritus food chain. The system omnivory index(SOI), Finn's cycled index(FCI) and connectance index(CI) were relatively low in this area while the total primary production/total respiration(TPP/TR) was high, indicating an immature and unstable status of the Jiaozhou Bay ecosystem. Mixed trophic impact analysis revealed that the cultured shellfish had substantial negative impacts on most functional groups. This study contributed to ecosystem-level evaluation and management planning of the Jiaozhou Bay ecosystem.

Evaluating impacts of pulse fishing on the effectiveness of seasonal closure

Seasonal fishing closures are often used in fisheries management to conserve overfished stocks.As one of the unintended consequences,fishermen often contend for maximizing catches immediately after reopening fisheries.The resultant large catch landings in a short time period(i.e.,pulse fishing)may undermine the benefit of closure.We implemented an end-to-end model OSMOSE-JZB(Object-oriented Simulator of Marine ec OSystem Exploitation OSMOSE)modelling ecosystem in the Jiaozhou Bay located in China to evaluate the impact of pulse fishing on the effectiveness of seasonal closure at levels of fish community,population,and individual.Our study demonstrated that the three-month closure was successful in conserving fish stocks.There were small variations on ecological indicators(i.e.,total biomass of the community,mean trophic level of the community,mean trophic level of the catch,and Shannon-Wiener biodiversity index)when pulse fishing occurred.Pulse fishing seemed not to result in a great shift in community structure.Compared to other species,the biomass of two large predatory fishes were more susceptible to pulse fishing.Pulse fishing could change the pressure of predators to fish stocks via food webs,especially for young individuals.Our simulations indicate that we can improve the effectiveness of seasonal closure by managing pulse fishing.Although the results derived in this study may be specific to the target ecosystem,the general approach is applicable to other ecosystems when evaluating fishing impacts.