Jiawei Xiaoyao San(JWXYS)has shown excellent clinical efficacy in anxiety disorder,but has not yet attracted widespread attention.The animal experiments,clinical trials and mechanism studies of JWXYS were reviewed in ...Jiawei Xiaoyao San(JWXYS)has shown excellent clinical efficacy in anxiety disorder,but has not yet attracted widespread attention.The animal experiments,clinical trials and mechanism studies of JWXYS were reviewed in this article,which may provide a reference for developing new anxiolytic drugs based on this prescription.The literature was searched in PubMed and CNKI and the documents written in English or with English abstracts were selected.JWXYS could reduce the anxiety symptoms of patients alone and reduce the adverse reactions when it is used in combination with other drugs in the clinic.In preclinical studies,JWXYS also showed therapeutic effects in reducing anxiety-like behavior.The mechanisms may include improving the hypothalamic–pituitaryadrenal(HPA)axis and hormone disorders,increasing neurotransmitter content,neurogenesis,and regulating the synthesis of related enzymes.This article shows that JWXYS could effectively treat anxiety disorders by regulating the central nervous system.In the future,with the participation of more researchers,it is expected to develop innovative drugs for the treatment of anxiety disorders based on JWXYS.展开更多
背景:课题组前期研究表明,加味逍遥散具有调节肝癌并发抑郁大鼠海马中多巴胺、五羟色胺含量的作用,同时能明显改善肝脏病理形态,改善大鼠的抑郁症候,但其药理机制尚未完全阐明。目的:通过全面而整体的蛋白质组学及网络药理学技术筛选出...背景:课题组前期研究表明,加味逍遥散具有调节肝癌并发抑郁大鼠海马中多巴胺、五羟色胺含量的作用,同时能明显改善肝脏病理形态,改善大鼠的抑郁症候,但其药理机制尚未完全阐明。目的:通过全面而整体的蛋白质组学及网络药理学技术筛选出加味逍遥散治疗肝癌并发抑郁模型大鼠的有效靶点,探讨加味逍遥散治疗肝癌并发抑郁症的活性成分及可能的作用机制。方法:构建肝癌并发抑郁症SD大鼠模型,行加味逍遥散干预治疗,对血清中的差异蛋白进行蛋白质组学与网络药理学预测靶点相互印证,对有效靶点进行基因本体论GO富集分析和KEGG富集分析;苏木精-伊红染色检测大鼠肝脏形态、行为学研究观察大鼠抑郁样行为的改变,酶联免疫吸附法测定相关靶点血清中的含量。结果与结论:①蛋白质组学及网络药理学获得3个可能共同蛋白靶点,分别为谷胱甘肽S转移酶μ重组蛋白(glutathione transferase,GSTM1)、3磷酸肌醇依赖性蛋白激酶1(3-phosphoinositide dependent protein kinase1,PDK1)、热休克蛋白90AB1(heat shock protein HSP 90-beta,HSP90AB1),GO富集分析与KEGG通路分析发现其直接参与神经元凋亡过程的负调控、同时涉及PI3K-Akt信号通路和前列腺癌分子信号通路。②苏木精-伊红染色和行为学检测表明加味逍遥散改善了大鼠肝脏损伤及抑郁样行为;酶联免疫吸附检测发现GSTM1、PDK1、HSP90AB1分别在加味逍遥散干预后具有纠正效果。③提示:加味逍遥散可能是通过影响GSTM1、PDK1、HSP90AB1表达纠正大鼠抑郁样行为,改善肝脏形态等作用发挥治疗肝癌并发抑郁症的作用。展开更多
基金supported by the National Natural Science Foundation of China(No.81173541).
文摘Jiawei Xiaoyao San(JWXYS)has shown excellent clinical efficacy in anxiety disorder,but has not yet attracted widespread attention.The animal experiments,clinical trials and mechanism studies of JWXYS were reviewed in this article,which may provide a reference for developing new anxiolytic drugs based on this prescription.The literature was searched in PubMed and CNKI and the documents written in English or with English abstracts were selected.JWXYS could reduce the anxiety symptoms of patients alone and reduce the adverse reactions when it is used in combination with other drugs in the clinic.In preclinical studies,JWXYS also showed therapeutic effects in reducing anxiety-like behavior.The mechanisms may include improving the hypothalamic–pituitaryadrenal(HPA)axis and hormone disorders,increasing neurotransmitter content,neurogenesis,and regulating the synthesis of related enzymes.This article shows that JWXYS could effectively treat anxiety disorders by regulating the central nervous system.In the future,with the participation of more researchers,it is expected to develop innovative drugs for the treatment of anxiety disorders based on JWXYS.
文摘背景:课题组前期研究表明,加味逍遥散具有调节肝癌并发抑郁大鼠海马中多巴胺、五羟色胺含量的作用,同时能明显改善肝脏病理形态,改善大鼠的抑郁症候,但其药理机制尚未完全阐明。目的:通过全面而整体的蛋白质组学及网络药理学技术筛选出加味逍遥散治疗肝癌并发抑郁模型大鼠的有效靶点,探讨加味逍遥散治疗肝癌并发抑郁症的活性成分及可能的作用机制。方法:构建肝癌并发抑郁症SD大鼠模型,行加味逍遥散干预治疗,对血清中的差异蛋白进行蛋白质组学与网络药理学预测靶点相互印证,对有效靶点进行基因本体论GO富集分析和KEGG富集分析;苏木精-伊红染色检测大鼠肝脏形态、行为学研究观察大鼠抑郁样行为的改变,酶联免疫吸附法测定相关靶点血清中的含量。结果与结论:①蛋白质组学及网络药理学获得3个可能共同蛋白靶点,分别为谷胱甘肽S转移酶μ重组蛋白(glutathione transferase,GSTM1)、3磷酸肌醇依赖性蛋白激酶1(3-phosphoinositide dependent protein kinase1,PDK1)、热休克蛋白90AB1(heat shock protein HSP 90-beta,HSP90AB1),GO富集分析与KEGG通路分析发现其直接参与神经元凋亡过程的负调控、同时涉及PI3K-Akt信号通路和前列腺癌分子信号通路。②苏木精-伊红染色和行为学检测表明加味逍遥散改善了大鼠肝脏损伤及抑郁样行为;酶联免疫吸附检测发现GSTM1、PDK1、HSP90AB1分别在加味逍遥散干预后具有纠正效果。③提示:加味逍遥散可能是通过影响GSTM1、PDK1、HSP90AB1表达纠正大鼠抑郁样行为,改善肝脏形态等作用发挥治疗肝癌并发抑郁症的作用。
