Analysis of fibronectin, fibronectin receptor and interleukin 1 in patients with cirrhosis treated by Yanggan Jieyu decoction

AIM To investigate the adjusting effects of the Yanggan Jieyu (YGJY, nourishing the liver and alleviate mental depression) decoction on the plasma concentration of fibronectin (FN), fibronectin receptor (FNR), tumor necrosis factor alpha (TNF α) and the activity of interleukin 1 (IL 1) in patients with cirrhosis. METHODS Thirty four cases of cirrhosis (in decompensation) were divided into YGJY decotion treated group and control group treated by routine method. FN, FNR and TNF α were measured with ELESA and expressed as mg/L (FN, FNR) and ng/L (TNF α), and IL 1 was measured by mice thymocyte proliferation using β scintillation counter and expressed as cpm. RESULTS In YGJY decoction treated group, before treatment, FN was 247 9±97 2, FNR 5 6±2 7, TNF α 83 9±7 1 and IL 1 was 2760 8±813 6, and after treatment. FN was 298 3±93 2 ( P <0 01), FNR 4 3±2 3 ( P <0 05, TNF α 93 6±12 0 ( P <0 05) and IL 1 was 1922 3±847 0 ( P <0 05). The FN and TNF α plasma level after treatment increased remarkably, while FNR and IL 1 decreased obviously. In the control group before treatment, FN was 248 8±101 9, FNR 5 5±1 9, TNF α 126 1±48 1 and IL 1 was 2540 6±603 2, and after treatment was 241 6±77 1 ( P >0 05), FNR 5 4±1 2 ( P >0 05), TNF α 100 6±15 5 ( P >0 05) and IL 1 was 2360 6±860 0 ( P >0 05), the plasma levels of FN, TNF α, FNR and IL 1 did not change signifiantly. CONCLUSION YGJY decoction could prevent the process of the hepatic fibrosis by readjusting the plasma levels of FN, FNR, TNF α and IL 1 mediating acivities in cirrhosis, which is of clinical significance.

He Jie Tang in the treatment of chronic hepatitis B patients

AIM： To explore the effect of He .lie Tang （decoction for medication） on serum levels of T lymphocyte subsets, NK cell activity and cytokines in chronic hepatitis B patients. METHODS： Eighty-five patients with chronic hepatitis B were divided randomly into two groups. Fifty patients in group I were treated with He .lie Tang （HIT） and 35 patients in group II were treated with combined medication. The levels of T-lymphocyte subsets （CD^3＋, CD^4＋, CD^8＋）, NK cell activity, cytokines （TNF-α, IL-8, sIL-2R） were observed before and after the treatment. Another 20 normal persons served as group 3. RESULTS： The level of CD^4＋ cells and NK cell activity were lower, whereas the level of CD^8＋ cells in patients was higher than that in normal persons （t = 2.685, 3.172, and 2.754 respectively; P〈0.01）. The levels of TNF-α, IL-8, and sIL-2R in chronic hepatitis B patients were higher than those in normal persons （t = 3.526, 3.170, and 2.876 respectively; P〈0.01）. After 6 months of treatment, ALT, AST, and TB levels in the two groups were obviously decreased （t = 3.421, 3.106, and 2.857 respectively; P〈0.01）. The level of CD^4＋ cells and NK cell activity were increased whereas the level of CD^8＋ cells decreased （t = 2.179, 2.423, and 2.677 respectively; P〈0.05） in group I. The levels of TNF-α, IL-8, and sIL- 2R in group I were decreased significantly after the treatment （t = 2.611, 2.275, and 2.480 respectively; P〈0.05） but had no significant difference in groupII after the treatment （t = 1.906, 1.833, and 2.029 respectively; P〉0.05）. The total effective rate had no significant difference between the two groups （X^2 = 2.882, P〉0.05） but the markedly effective rate was significantly different between the two groups （X^2 = 5.340, P〈0.05）. CONCLUSION： HIT is effective in treating chronic hepatitis B. HIT seems to exert its effect by improving the cellular immune function and decreasing inflammatory cytokines in chronic hepatitis B patients. The function of HIT in protecting liver function in the process of eliminating virus needs to be further studied.

联合信息环境JIE及其安全研究

全球信息栅格(GIG)是美军为网络中心战建设的重要基础设施,而联合信息环境(JIE)是美军基于安全性考虑,对整个GIG重新设计而提出的,它将成为GIG的下一代战略储备。文中跟踪了JIE的研究进展,挖掘出美军提出JIE的深层原因,分析了JIE的特点、关键建设领域、理想的终极状态。同时,对JIE的统一的安全体系结构和统一的身份和访问管理解决方案进行了剖析,文中最后全面深入地研究了JIE的安全功能。

Compound Chai Jin Jie Yu Tablets,Acts as An Antidepressant by Promoting Synaptic Function in the Hippocampal Neurons

Objective To investigate the effectiveness of compound Chai Jin Jie Yu Tablets(CJJYT)in ameliorating cognitive impairment associated with depression and its potential mechanism of action.Methods In vitro experiments,the hippocampus was isolated from the whole brain of the fetal rat and cultured into hippocampal neuron cells.50μM corticosterone(CORT)was added to each group 18 h before the experiment for modeling depression,with the exception of the control group.After modeling,the blank serum group was added with 10%blank serum,the CJJYT group and the venlafaxine group were added with the corresponding 10%drug-containing serum,and the control group and the model group were added with equal volumes of culture medium.The intracellular Ca^2+mean fluorescence intensity,miniature excitatory postsynaptic current(mEPSC)current amplitude,and frequency of different hippocampal neurons were evaluated as indicators of synaptic function in the hippocampal neurons.In addition,the expression of synaptic plasticity related proteins,synaptophysin-α(SYN-α),N-methyl-D-aspartate receptor 2A(NR2A),N-methyl-Daspartate receptor 2B(NR2B),post synaptic density 95 protein(PSD-95),calcium/calmodulin dependent protein kinaseⅡ(CaMKⅡ)and synaptic associated protein(SynGAP)were detected in the hippocampal neurons by immunofluorescence staining and high content analysis(HCA)system.Then,reverse transcription-polymerase chain reaction(RT-PCR)was used to detect the mRNA expression levels of SYN-α,NR2A,NR2B,PSD-95,CaMKⅡand SynGAP.For in vivo experiments,except for those in the blank control group,all rats were treated within a single cage for chronic unpredictable stress-induced depression modeling and subjected to corresponding drug interventions.Behavioral tests were used to detect depressive behavior and determine learning,memory and other cognitive abilities,whereas enzyme-linked immunosorbent assay(ELISA)was used to detect the CORT levels.Golgi-Cox staining was used to observe changes in the synaptic morphology of parahippocampal gyrus CA1 area(CA1)and dentategyrus(DG).Results In vitro,CJJYT treatment reduced the intracellular Ca^2+mean flurorescence intensity in the hippocampal neurons(P<0.05),causing a reduction in the frequency and current amplitude of mEPSC(P<0.05),and thus inhibited the excessive activation of post-synaptic receptors.CJJYT treatment reduced the protein and mRNA expression of SYN-α,NR2A,NR2B and PSD-95 in the hippocampal neurons(P<0.05),increased the mRNA and protein expression of CaMKⅡand SynGAP(P<0.05),and thereby improved the synaptic plasticity of the hippocampal neurons.In vivo,CJJYT intervention improved sucrose preference,voluntary activity,learning and memory ability of Morris water maze test,and suppressed appetite(P<0.05),and increased the despair feeling of forced swimming test(P<0.05).The CORT level was reduced(P<0.05),leading to the repair of synaptic damage in the hippocampal neurons.Conclusions CJJYT can improve the synaptic function of hippocampal neurons and has obvious protective effects on neurons.It can repair the structural damage in the hippocampal neurons,improving the cognitive ability of the depressed model rats.The mechanism of CJJYT improving cognition in depressed rats may be related to the transmission and function of SYN-α/NR and its downstream neurotransmitters.

Yi Qi Jie Du Formula and Salinomycin Combination Treatment Mediates Nasopharyngeal Carcinoma Stem Cell Proliferation,Migration and Apoptosis via CD44/Ras Signaling Pathway

Objective To assess the effects of Yi Qi Jie Du Formula(YQJDF)combined with salinomycin(SAL)on nasopharyngeal carcinoma stem cells(NPC-SCs)and investigate the underlying molecular mechanisms.Methods Cell counting methods,the CCK-8 assay,transwell migration assay and JC-1 staining,were used to observe the effects of the combination on the proliferation,migration and apoptosis of NPC stem cells,respectively.Western blot was used to detect the levels of protein in NPC-SCs.Results YQJDF combined with SAL had a synergistic effect on the inhibition of proliferation and migration and induction of NPCSC apoptosis.Mechanistically,YQJDF combined with SAL synergistically upregulated the levels of apoptotic proteins,including cleaved Caspase-3,cleaved Caspase-7 and cleaved Caspase-9.Moreover,YQJDF combined with SAL synergistically decreased the levels of CD44,p-c-Src,Ras,p-PKCδ,p-MEK,p-c-Raf,p-ERK1/2 and p-AKT proteins.Conclusions The combination of YQJDF and SAL has a synergistic effect on the inhibition of NPC-SC proliferation and migration and induction of apoptosis,which may be closely related to the downregulation of the CD44/Ras signaling pathway.

Yi Qi Jie Du Decoction Inhibits Proliferation and Induces Apoptosis of Nasopharyngeal Carcinoma Stem Cells Through Mitochondrial Apoptosis Pathway

Objective To assess the effects of Yi Qi Jie Du Decoction(YQJDD)on nasopharyngeal carcinoma stem cells(NPC-SCs)and investigate the underlying mechanism.Methods NPC-SCs were collected in serum-free culture system and identified by the sphere formation assay.The effect of YQJDD on the proliferation of NPC-SCs was detected using the Cell Counting Kit-8 assay.The change of mitochondrial membrane potential(ΔΨm)in NPC-SCs following treatment with YQJDD was investigated by JC-1 staining.The levels of apoptosisassociated proteins were examined using western blot analysis.Results YQJDD significantly inhibited the proliferation of NPCSCs in dose-and time-dependent manners.JC-1 staining revealed that YQJDD lowered theΔΨm of NPC-SCs in a timedependent manner.After treatment with YQJDD,the expression of cleaved caspase-3,-7 and-9,cleaved poly-ADP ribose polymerase,P21 and P53 were increased,while the expression of Survivin in NPC-SCs was decreased.However,the expression of cleaved caspase-8 remained nearly unchanged.Conclusions YQJDD inhibits the growth and induces apoptosis of NPC-SCs via an intrinsic apoptosis pathway.

熠熠星光,成就“Jie”少年——谈我班特色中队建设中的评价机制

我国著名经济学家李开复曾说过:严管中长大的孩子,无法独立;施压中长大的孩子,常常忧虑;信赖中长大的孩子,信人信己;放权中长大的孩子,深具责任。是啊,在孩子的成长道路上,师长的溺爱不仅仅剥夺了孩子真正长大成人的权利,也剥夺了孩子在成长道路的"酸、甜、苦、辣"。这样的孩子永远都是长不大的。所以,在我们"Jie"中队的创建过程中我始终让他们在自我评价和小组竞争评价中,认识自我,塑造自我,最终成就自我。

Jie Le Brand Baby-use Articles

The Jie Le Brand articles for baby use, including Silastic teats and milk bottles, produced by Jinan Jialin Silastic Products Co. Ltd. are made with imported pure materials without any harmful content such as nitrosamine. A Sinoforeign joint venture specializing in Silastic product production using firstrate productive technology and conditions, the company’s main equipment has all been imported from abroad, and its products are exported to various parts of the world.

Minister Hou Jie on China's Housing Construction and Urban Development

At a reception held recently, Mr. Hou Jie, Minister of Construction, spoke about China’s housing construction and urban development. According to Mr. Hou Jie, the Chinese government always pays high attention to housing development, and has adopted many policies to speed up the construction of houses. Especially since 1979 when China introduced the policy of reform and opening, residential housing has developed rapidly and witnessed outstanding achievement. From 1979 to 1996, 13.8 billion square metres of residential

Keep One Step Ahead and Face the Future——Chen Jie,Vice President of ZTE Corporation,Talks About ZTE's NGN Development Strategy

Editor’s Note:In the recent two yearssince ZTE’s NGN prod-uct series were put intomarket,ZTE has maderemarkable achieve-ments in the industry. Al-though a leading vendorin China, ZTE has beencontinuously doing busi- ness in a prudent and practical way,which has been recognized by govern-ment authorities, carriers and customers.In order to learn more about ZTE’s NGNdevelopment strategy,we interviewedChen Jie, ZTE’s vice president and gen-eral manager of Network Division, aboutZTE’s NGN development strategy, R&Dadvantages and progress.

‘Reversing’the Flow of the Ancient Chinese Calendrical Subseasonal Phases Jie Qi in Australia:The Construction of a Chinese Medical & Agricultural Lunisolar Calendar ( Northern and Southern Hemispheres)(Continued)

Jie qi(subseasonal phases or solar qi)constitutes the solar component of the ancient Chinese lunisolar calendar. It divides the year into twenty-four subseasonal phases i.e. li chun(beginning of spring),chun fen(spring equinox),etc.From the premodern perspective of Traditional Chinese Medicine(TCM),normal climate change(qi hou bian qian)moves in harmony with the temporal flow of the qi(qi yun)and is thus beneficial to human health.However,abnormal,abrupt weather or climatic changes like those weather features of being too cold or too hot,too windy,too humid,too dry or a combination of two or more of these weather/climatic features which occur out of season can lead to'seasonal diseases'(shi bing)or 'diseases brought about by external influences'(wai gan zhi bing)or by the'six excessive climatic qi etiological factors'(liu yin zhi bing 六因致病).Some examples of seasonal diseases are SARS,influenza,the common cold and heat stroke. But the temporal flow of the subseasonal qi(qi yun) in Australia is the reverse of that of the northern hemispherical regions of China,United States and Europe.This situation thus leads to a temporal desynchronisation of TCM practice and practitioners with the rhythm of the seasons.In order to address this problem,I have constructed a Chinese Medical & Agricultural Lunisolar Calendar (Northern & Southern Hemispheres),which'reverses'the flow of the subseasonal phases in the southern hemisphere.Having this calendrical tool,Chinese medicine practitioners in Australia can'reverse'their clinical activities in accordance with the natural rhythm of the seasons here.

《黄帝内经》节字含义缕析

目的基于语料库证据,通观解释《黄帝内经》中“节”字的含义,分类整理探析“节”的内涵。方法采用文献计量学的研究方法,对《黄帝内经》中的“节”字进行检索、统计、分析,对“节”字的数量、分布、功能等进行定量、定性汇总。结果《黄帝内经》中的“节”动静皆宜,动指调节生命的节律,静则具象为关节骨节,与天人相应理论相合。结论《黄帝内经》时代对“节”的理解基于天人相应理论,现代趋向于将“节”字的含义延伸至生命节律、神经通路等多元化高级通路的概念层面。

Effects of Shang Ke Jie Gu tablet on fracture healing in rabbits

Objective:To study the effects of Shang Ke Jie Gu tablet on fracture healing in rabbits.Methods:40 New Zealand rabbits,half male and half female,were randomly divided into two groups:normal saline group(N group)and Shang Ke Jie Gu tablet group(S group).Each group consisted of 20 rabbits.Left radial fracture models in upper and middle sections were made in rabbits and then intra-gastric administration was given.Five rabbits in each group were killed on the 10 th,20^(th),30^(th) and 40^(th) day after surgery,left radius was taken to get an X-ray.The degree of healing was assessed by the evaluation criteria of Shanghai Orthopaedics Institute.Results:The degree of fracture healing in S group was higher in the comparison with that in N group.Conclusions:Shang Ke Jie Gu tablet might affect endochondral ossification during fracture healing of rabbits,and promote porosis and fracture healing.

DK音响进驻吉林市JIE JIE慢摇吧

JIEJIE慢摇吧是吉林市高档娱乐场所之一，其音响系统由DK音响吉林市代理商一一神龙音响公司完成。作为一座纯娱乐与交友慢摇性质的娱乐场所，该慢摇吧的音乐为HITP的路线及最流行的电子音乐风格，欧洲文化的音乐设计为慢摇吧增添了独特的创意。设计中，DK音响吉林市代理商神龙音响公司既考虑到DANCE周围高声压区域的要求，

Story of Hou Jie

In Connection with Surroundina 10 Cities When talks about Hou Jie, Chen Zhongqiu, President of Guangdong Modern International Exhibition Center firstly mentioned location of Hou Jie. Hou Jie may cover 10 cities including Hong Kong, Guangzhou, Shenzhen, Foshan, Zhongshan, Zhuhai, Huizhou,

四“jie”助我做好班主任

在当今社会,人们对教师的要求越来越高,班主任又由于其特殊性,要求更高。班级管理事无巨细,是一项纷繁复杂而又妙趣横生的工作。它的成功与否直接影响着学生的品行、学业和前途,也影响着学校的地位、声誉和未来。因此,做好班主任工作对学生在德、智。

‘Reversing’ the Flow of the Ancient Chinese Calendrical Subseasonal Phases Jie Qi in Australia:The Construction of a Chinese Medical & Agricultural Lunisolar Calendar (Northern and Southern Hemispheres) (Continued Part 1)

1 The Cyclical Flow of the Sixty-Gan zhi Temporal Units 'Time for the Chinese is forever flowing without beginning or end',noted Thome H. Fang. And'it is customary for Chinese to use the kan-Chih(gan zhi)to mark the passage of time.There are ten heavenly stems(gan)and twelve earthly branches(zhi),an alternating and sequential combination of the two makes a cycle of sixty years,months,days and two-hour period shi chen(时辰)in a day'. The sixty gan zhi temporal cycle is very important in the practice of Chinese medicine.It is an important practical temporal tool in the acupuncture practice of 子午流注(zi wu liu zhu)which is a method of choosing and using acupuncture points in dealing with a specific clinical pattern in accordance with the patient's temporal clotting and flowing of his/her Qi and blood.In addition,the sixty gan-zhi temporal cyclical system is also an important tool in forecasting adverse weather/climactic conditions and the illnesses that comes with them on the basis of the ancient methodology of 'the circulating five elements/phases and the six climactic influences'五运六气.Hence,I have included this gan zhi or celestial stems and earthly branches for each of the days in the Chinese Medical and Agricultural Lunisolar Calendar for the year of Rat wu zi nian(wu is the celestial stem while zi is the earthly branch for the year 2008).

仓颉--突破表达困境的圣人

汉字是中国文明的首要标志。仓颉造字,华夏文明由此破局,也构成中国文学的开端。文字的发明,解决了文明体自我表达与自我积累问题,提升了人们的文学表达能力、人才识别能力和社会组织能力。在黄帝时代文明涌生的背景上,仓颉担任黄帝的左史,造字既是黄帝事业的重要组成部分,也是黄帝事业能产生重大影响的关键因素。

论小资产阶级文艺的论争与批判——从许杰的《小资产阶级与文艺》一文说起

1957年8月,叶以群、孔罗荪、姚文元合作撰文《许杰在文艺上政治上的反动道路》,其中叶以群的《许杰的“小资产阶级文艺”之旗》,直接批判许杰1948年发表的《小资产阶级与文艺》。1928年的革命文学倡导,开启了以阶级划分作家、以小资产阶级界定作家与创作的先声。在与梁实秋的人性论、“自由人”、“第三种人”的论争中,常常将他们归为小资产阶级文人,成为找不到政治出路动摇者的标签。1942年延安文艺座谈会后,知识分子的思想改造被确定了,小资产阶级的自我反省和批判被突出了。在中国现代文学史中,常常以小资产阶级作为评判作家创作的不足和短处。在历次对于小资产阶级文人和创作的批判针砭过程中,将阶级斗争观念作为衡量作家衡量文学的标准,忽略与抛弃了人性与文学的复杂性和丰富性;将革命与否视作评判作家立场与情感的唯一标准,模糊与消弭文学与革命不同的视阈及文学的特性;将工农兵的立场和情感看作衡量作家和文学的标准,批评或否定了文学表达的多样性与先锋性。