Comparative study on the anti-tuberculous effect and mechanism of the traditional Chinese medicines NiuBeiXiao He extract and JieHeWan

Background:The traditional Chinese medicine NiuBeiXiaoHe(NBXH)extract and Chinese medicine preparation JieHeWan(JHW)exhibit anti-tuberculosis(TB)effects.The anti-TB effect of NBXH was compared with that of JHW to elucidate the mechanism of action of NBXH.Methods:BALB/c mice aged 6-8 weeks were randomly divided into a normal control group,TB model group,JHW treatment group,and NBXH treatment group.After 3 and 13 weeks of treatment,the therapeutic effect in each group was evaluated by comparing lung histopathology,lung and liver colony counts,the number of spots representing effector T cells secreting IFN-γin an enzyme-linked immunospot assay(ELISPOT),and the levels of Th1,Th2,and Th17 cytokines,which were measured by a cytometric bead array(CBA).Mouse RNA samples were subjected to transcriptome sequencing.Results:After 13 weeks of treatment,the mean histopathological lesion area of the NBXH group was significantly smaller than that of the TB model group(P<0.05).Compared with those in the TB model group,the lung colony counts in the JHW and NBXH groups were significantly decreased(P<0.05),and the IL-2 and IL-4 levels in the NBXH group were significantly increased(P<0.05).NBXH partly restored significant changes in gene expression caused by Mycobacterium tuberculosis(M.tuberculosis)infection.According to GO and KEGG analyses,the changes in biological process(BP),cell composition(CC)and molecular function(MF)terms and in signaling pathways caused by NBXH and JHW treatment were not completely consistent,but they were mainly related to the immune response and inflammatory response in the mouse TB model.Conclusions:NBXH had therapeutic effects similar to those of JHW in improving lung histopathology,reducing lung colony counts,and regulating the levels of cytokines.NBXH restored significant changes in gene expression and repaired cell damage caused by M.tuberculosis infection by regulating immune-related pathways,which clarified the mechanism of action of NBXH.

中药结核丸辅助治疗初患涂阳肺结核

目的探讨中药结核丸辅助治疗初患涂阳肺结核的临床效果。方法按随机数字法将78例初患涂阳肺结核患者分为对照组和治疗组,对照组38例采用2HRZE/4HR方案治疗,治疗组40例在对照组治疗基础上,加用中药结核丸(由生地黄、熟地黄、天冬、麦冬、北沙参、龟甲、牡蛎、百部、白及、鳖甲等16味中药组成)治疗。比较强化期治疗结束后2组临床症状、痰菌阴转、病灶吸收情况及不良反应。结果治疗组在临床症状改善、痰菌阴转及病灶吸收等方面明显优于对照组(P<0.05);治疗组不良反应发生率明显低于对照组(P<0.05)。结论中药结核丸具有滋阴润肺补肾、扶正固本,抗杀痨虫之功效,辅助治疗初患涂阳肺结核,能加快痰菌阴转速度,促进病灶吸收。