Objective To investigate the efficacy and mechanism of action of Compound Chaijin Jieyu Tablets(复方柴金解郁片,CCJJYT)in rats with insomnia complicated with depression.Methods Seventy-two Sprague-Dawley rats were rand...Objective To investigate the efficacy and mechanism of action of Compound Chaijin Jieyu Tablets(复方柴金解郁片,CCJJYT)in rats with insomnia complicated with depression.Methods Seventy-two Sprague-Dawley rats were randomly assigned into eight groups:the control,chronic unpredictable mild stress(CUMS),sleep deprivation(SD),CUMS+SD,positive drug(venlafaxine hydrochloride+diazepam),CCJJYT high-dose(CCJJYT˗2×),medium-dose(CCJJYT˗1×),and low-dose(CCJJYT˗0.5×)groups,with nine rats in each group.Depression-like behavior was evaluated by body weight,food intake,and behavioral tests such as the sucrose preference test(SPT),open field test(OFT),forced swimming test(FST),and pentobarbital-induced sleep test(PST).Hematoxylin-eosin(HE)staining and Golgi-Cox staining were used to observe changes in pathological tissue and synaptic morphology,respectively.Enzyme-linked immunosorbent assay(ELISA)was used to detect the contents of orexin-A and acetylcholine.The expression levels of orexin receptor 1(OXR1),melatonin receptor 1(MT1A),melatonin receptor 2(MT1B),acetylcholinesterase(AChE),and choline acetyltransferase(ChAT)were detected by immunohistochemistry and Western blot.Results In the present study,rats in the model group showed significant behavioral changes as well as a reduction in hippocampal dendritic branch length and synaptic number,along with increasing the content of orexin A and acetylcholine(P<0.05),and altered expression levels of OX1R,MT1A,MT1B,ChAT,and AChE in the hippocampus and prefrontal cortex after modeling(P<0.05).CCJJYT can improve depressive insomnia behavior and synaptic plasticity of rats(P<0.05),which is similar to that of the positive drug group.It can also decrease the content of orexin A and acetylcholine,and reduce the expression levels of OXR1 and ChAT in hippocampus and prefrontal cortex(P<0.05),and increase the expression levels of MT1A,MT1B,and AChE proteins(P<0.05).Conclusion CCJJYT has good antidepressant and insomnia effects,probably through the regulation of orexin-A,melatonin,and acetylcholine content in hippocampus and prefrontal cortex of rats,improving synaptic plasticity and thus exerting antidepressant and insomnia effects.展开更多
BACKGROUND To assess the effectiveness of Shugan Jieyu capsules on peripheral blood miR-124,miR-132,and brain-derived neurotrophic factor(BDNF)levels in patients with mild to moderate depression following coronary art...BACKGROUND To assess the effectiveness of Shugan Jieyu capsules on peripheral blood miR-124,miR-132,and brain-derived neurotrophic factor(BDNF)levels in patients with mild to moderate depression following coronary artery intervention[percuta-neous coronary intervention(PCI)]for coronary heart disease.Patients with mild-to-moderate depression of the liver-qi stagnation type after PCI for coronary heart disease at the 305th Hospital of the People’s Liberation Army were enrolled from June 2022 to November 2023 and randomly assigned to two groups:Experimental(treated with Shugan Jieyu capsules)and control(tr-eated with escitalopram oxalate tablets).This study compared the antidepressant effects of these treatments using 17-item Hamilton Rating Scale for Depression(HAMD-17)scores,metabolic equivalents,low-density lipoprotein cholesterol,BDNF,high-sensitivity C-reactive protein levels,miR-124 and miR-132 levels,distribution of immune-related lymphocyte subsets,and traditional Chinese me-dicine syndrome scores before and after 6 weeks of treatment.RESULTS No significant difference was observed in any index between the two groups before treatment(P>0.05).After treatment,the total efficacy rates were 93.33%and 90.00%in the experimental and control groups,respectively.Experimental group had significantly lower scores for the main and secondary syndromes compared to the control group(P<0.05).No significant difference was observed in the metabolic equivalents between the two groups be-fore and after treatment(P>0.05).The levels of low-density lipoprotein cholesterol,high-sensitivity C-reactive pro-tein,and miR-132 were significantly lower,whereas those of miR-124,BDNF,CD3+T lymphocytes,CD3+CD4+T helper lymphocytes,and CD3+CD4+/CD3+CD8+cells were significantly higher in the experimental group com-pared to the control group(P<0.05).The incidence of adverse reactions during experimental group was signi-ficantly lower than that in control group(P<0.05).CONCLUSION Shugan Jieyu capsules have good efficacy in patients with mild-to-moderate depression after PCI,and its me-chanism may contribute to the regulation of miR-124,miR-132,BDNF levels,and lymphoid immune cells.展开更多
[Objectives]To investigate the efficacy of Anshen Jieyu decoction for sub-healthy people with insomnia.[Methods]A total of 80 sub-healthy insomnia patients admitted to Taihe Hospital of Shiyan City from January,2020 t...[Objectives]To investigate the efficacy of Anshen Jieyu decoction for sub-healthy people with insomnia.[Methods]A total of 80 sub-healthy insomnia patients admitted to Taihe Hospital of Shiyan City from January,2020 to December,2020 were selected as the research objects.All the patients were divided into observation group and control group according to a random number table.The control group was treated with conventional Western medicine,and the observation group was treated with Anshen Jieyu decoction.The clinical treatment effect of the two groups was compared.[Results]Before the intervention,there was no significant difference in Pittsburgh sleep quality index between the two groups.After the intervention,the scores of time required to fall asleep[(1.2±0.3)points],sleep time[(1.2±0.4)points],sleep disorder[(1.2±0.2)points],sleep efficiency[(1.4±0.3 points)],sleep quality[(0.9±0.2)points]and daytime dysfunction[(0.9±0.4)points]of the observation group were significantly lower than those of the control group.There was no significant difference in life quality between the two groups before the intervention.The scores of physical function[(92.3±3.3)points],social function[(91.7±4.1)points],mental health[(92.3±4.1)points]and emotional role[(92.8±5.2)points]after the intervention were significantly higher than those before the intervention.Before the intervention,there was no significant difference in the severity of insomnia between the two groups.After the intervention,the score of insomnia severity index was(10.3±3.0)points in the observation group and was(12.2±2.9)points in the control group.In terms of safety,there were 23 cases of level 1,15 cases of level 2 and 2 cases of level 3 in the observation group,and there were 20 cases of level 1,16 cases of level 2 and 4 cases of level 3 in the control group.No significant difference was observed in treatment safety between the two groups.[Conclusions]Anshen Jieyu decoction is effective in treating sub-healthy insomnia and is worthy of promotion.展开更多
OBJECTIVE To explore the effects and underlying mechanism of Jieyu Anshen granule(JY) in chronic unpredictable mild stress(CUMS)-treated rats after ischemic stroke.METHODS A rat model of post-stroke depression(PSD) wa...OBJECTIVE To explore the effects and underlying mechanism of Jieyu Anshen granule(JY) in chronic unpredictable mild stress(CUMS)-treated rats after ischemic stroke.METHODS A rat model of post-stroke depression(PSD) was developed by additional CUMS procedures after middle cere.bral artery occlusion(MCAO).Sprague-Dawley rats were given 1 g·kg^(-1) and 3 g·kg^(-1) of JY by gastrogavage for 4 weeks.Escitalopram(10 mg·kg^(-1)) served as a reference drug.Behavioral tests including sucrose preference test,forced swim test and open-field test were performed to evaluate the antidepressant effects.Levels of norepinephrine(NE),dopamine(DA) and 5-hydroxytryptamine(5-HT) in rat brain were assayed.The anti-inflammatory activity was evaluated by measuring TNF-α and IL-1β in brain.Serum adrenocorticotropic hormone(ACTH) and corticosterone(CORT) were estimated as indices of hypothalamic-pituitary-adrenal(HPA) axis activity.Western blot analysis was used to evaluate hippo.campal expression of the 5-HT1 A receptor(5-HT_(1A)R) and brain-derived neurotrophic factor(BDNF).RESULTS PSD rats exhibited decreased sucrose consumption and motor activity,increased immobility time(P<0.01).JY treatment reversed the depressive behaviors in PSD rats(P<0.05,P<0.01).Treat.ment with JY resulted in significantly increased levels of NE,DA and 5-HT in the hippocampus and prefrontal cortex(P<0.05,P<0.01),and increased expression of 5-HT_(1A)R and BDNF in the hippocampus(P<0.01).JY treatment significantly down-regulated the levels of TNF-α and IL-1β in hippocampus and prefrontal cortex(P<0.05).Treatment with JY also resulted in significantly decreased ACTH and CORT in serum which had been increased(P<0.05).CONCLUSION These findings suggest that JY treat.ment could ameliorate PSD,and the effects are likely ascribed to inhibiting HPA axis hyperfunction and inflammatory,up-regulating the levels of neurotransmitters(NE,DA and 5-HT),and the expression of hippocampal 5-HT_(1A)R and BDNF.展开更多
基金National Major New Drug Development Project(2017 ZX09309026)National Natural Science Foundation of China(82104846)+1 种基金Natural Science Foundation of Hunan Province(2022JJ40323 and 2022JJ80092)Natural Science Foundation of Changsha(Kq2202266).
文摘Objective To investigate the efficacy and mechanism of action of Compound Chaijin Jieyu Tablets(复方柴金解郁片,CCJJYT)in rats with insomnia complicated with depression.Methods Seventy-two Sprague-Dawley rats were randomly assigned into eight groups:the control,chronic unpredictable mild stress(CUMS),sleep deprivation(SD),CUMS+SD,positive drug(venlafaxine hydrochloride+diazepam),CCJJYT high-dose(CCJJYT˗2×),medium-dose(CCJJYT˗1×),and low-dose(CCJJYT˗0.5×)groups,with nine rats in each group.Depression-like behavior was evaluated by body weight,food intake,and behavioral tests such as the sucrose preference test(SPT),open field test(OFT),forced swimming test(FST),and pentobarbital-induced sleep test(PST).Hematoxylin-eosin(HE)staining and Golgi-Cox staining were used to observe changes in pathological tissue and synaptic morphology,respectively.Enzyme-linked immunosorbent assay(ELISA)was used to detect the contents of orexin-A and acetylcholine.The expression levels of orexin receptor 1(OXR1),melatonin receptor 1(MT1A),melatonin receptor 2(MT1B),acetylcholinesterase(AChE),and choline acetyltransferase(ChAT)were detected by immunohistochemistry and Western blot.Results In the present study,rats in the model group showed significant behavioral changes as well as a reduction in hippocampal dendritic branch length and synaptic number,along with increasing the content of orexin A and acetylcholine(P<0.05),and altered expression levels of OX1R,MT1A,MT1B,ChAT,and AChE in the hippocampus and prefrontal cortex after modeling(P<0.05).CCJJYT can improve depressive insomnia behavior and synaptic plasticity of rats(P<0.05),which is similar to that of the positive drug group.It can also decrease the content of orexin A and acetylcholine,and reduce the expression levels of OXR1 and ChAT in hippocampus and prefrontal cortex(P<0.05),and increase the expression levels of MT1A,MT1B,and AChE proteins(P<0.05).Conclusion CCJJYT has good antidepressant and insomnia effects,probably through the regulation of orexin-A,melatonin,and acetylcholine content in hippocampus and prefrontal cortex of rats,improving synaptic plasticity and thus exerting antidepressant and insomnia effects.
基金the 305 Hospital Independent Scientific Research Fund,2024,No.24ZZJJLW-022.
文摘BACKGROUND To assess the effectiveness of Shugan Jieyu capsules on peripheral blood miR-124,miR-132,and brain-derived neurotrophic factor(BDNF)levels in patients with mild to moderate depression following coronary artery intervention[percuta-neous coronary intervention(PCI)]for coronary heart disease.Patients with mild-to-moderate depression of the liver-qi stagnation type after PCI for coronary heart disease at the 305th Hospital of the People’s Liberation Army were enrolled from June 2022 to November 2023 and randomly assigned to two groups:Experimental(treated with Shugan Jieyu capsules)and control(tr-eated with escitalopram oxalate tablets).This study compared the antidepressant effects of these treatments using 17-item Hamilton Rating Scale for Depression(HAMD-17)scores,metabolic equivalents,low-density lipoprotein cholesterol,BDNF,high-sensitivity C-reactive protein levels,miR-124 and miR-132 levels,distribution of immune-related lymphocyte subsets,and traditional Chinese me-dicine syndrome scores before and after 6 weeks of treatment.RESULTS No significant difference was observed in any index between the two groups before treatment(P>0.05).After treatment,the total efficacy rates were 93.33%and 90.00%in the experimental and control groups,respectively.Experimental group had significantly lower scores for the main and secondary syndromes compared to the control group(P<0.05).No significant difference was observed in the metabolic equivalents between the two groups be-fore and after treatment(P>0.05).The levels of low-density lipoprotein cholesterol,high-sensitivity C-reactive pro-tein,and miR-132 were significantly lower,whereas those of miR-124,BDNF,CD3+T lymphocytes,CD3+CD4+T helper lymphocytes,and CD3+CD4+/CD3+CD8+cells were significantly higher in the experimental group com-pared to the control group(P<0.05).The incidence of adverse reactions during experimental group was signi-ficantly lower than that in control group(P<0.05).CONCLUSION Shugan Jieyu capsules have good efficacy in patients with mild-to-moderate depression after PCI,and its me-chanism may contribute to the regulation of miR-124,miR-132,BDNF levels,and lymphoid immune cells.
基金Supported by Project of Taihe Hospital of Shiyan City(2019JJXM099).
文摘[Objectives]To investigate the efficacy of Anshen Jieyu decoction for sub-healthy people with insomnia.[Methods]A total of 80 sub-healthy insomnia patients admitted to Taihe Hospital of Shiyan City from January,2020 to December,2020 were selected as the research objects.All the patients were divided into observation group and control group according to a random number table.The control group was treated with conventional Western medicine,and the observation group was treated with Anshen Jieyu decoction.The clinical treatment effect of the two groups was compared.[Results]Before the intervention,there was no significant difference in Pittsburgh sleep quality index between the two groups.After the intervention,the scores of time required to fall asleep[(1.2±0.3)points],sleep time[(1.2±0.4)points],sleep disorder[(1.2±0.2)points],sleep efficiency[(1.4±0.3 points)],sleep quality[(0.9±0.2)points]and daytime dysfunction[(0.9±0.4)points]of the observation group were significantly lower than those of the control group.There was no significant difference in life quality between the two groups before the intervention.The scores of physical function[(92.3±3.3)points],social function[(91.7±4.1)points],mental health[(92.3±4.1)points]and emotional role[(92.8±5.2)points]after the intervention were significantly higher than those before the intervention.Before the intervention,there was no significant difference in the severity of insomnia between the two groups.After the intervention,the score of insomnia severity index was(10.3±3.0)points in the observation group and was(12.2±2.9)points in the control group.In terms of safety,there were 23 cases of level 1,15 cases of level 2 and 2 cases of level 3 in the observation group,and there were 20 cases of level 1,16 cases of level 2 and 4 cases of level 3 in the control group.No significant difference was observed in treatment safety between the two groups.[Conclusions]Anshen Jieyu decoction is effective in treating sub-healthy insomnia and is worthy of promotion.
基金supported by National Natural Science Foundation of China(8157369)
文摘OBJECTIVE To explore the effects and underlying mechanism of Jieyu Anshen granule(JY) in chronic unpredictable mild stress(CUMS)-treated rats after ischemic stroke.METHODS A rat model of post-stroke depression(PSD) was developed by additional CUMS procedures after middle cere.bral artery occlusion(MCAO).Sprague-Dawley rats were given 1 g·kg^(-1) and 3 g·kg^(-1) of JY by gastrogavage for 4 weeks.Escitalopram(10 mg·kg^(-1)) served as a reference drug.Behavioral tests including sucrose preference test,forced swim test and open-field test were performed to evaluate the antidepressant effects.Levels of norepinephrine(NE),dopamine(DA) and 5-hydroxytryptamine(5-HT) in rat brain were assayed.The anti-inflammatory activity was evaluated by measuring TNF-α and IL-1β in brain.Serum adrenocorticotropic hormone(ACTH) and corticosterone(CORT) were estimated as indices of hypothalamic-pituitary-adrenal(HPA) axis activity.Western blot analysis was used to evaluate hippo.campal expression of the 5-HT1 A receptor(5-HT_(1A)R) and brain-derived neurotrophic factor(BDNF).RESULTS PSD rats exhibited decreased sucrose consumption and motor activity,increased immobility time(P<0.01).JY treatment reversed the depressive behaviors in PSD rats(P<0.05,P<0.01).Treat.ment with JY resulted in significantly increased levels of NE,DA and 5-HT in the hippocampus and prefrontal cortex(P<0.05,P<0.01),and increased expression of 5-HT_(1A)R and BDNF in the hippocampus(P<0.01).JY treatment significantly down-regulated the levels of TNF-α and IL-1β in hippocampus and prefrontal cortex(P<0.05).Treatment with JY also resulted in significantly decreased ACTH and CORT in serum which had been increased(P<0.05).CONCLUSION These findings suggest that JY treat.ment could ameliorate PSD,and the effects are likely ascribed to inhibiting HPA axis hyperfunction and inflammatory,up-regulating the levels of neurotransmitters(NE,DA and 5-HT),and the expression of hippocampal 5-HT_(1A)R and BDNF.
