A rat model of middle cerebral artery permanent occlusion was established using the modified Longa method. Successfully established model animals were treated by blood-letting puncture at twelve Jing-Well points of th...A rat model of middle cerebral artery permanent occlusion was established using the modified Longa method. Successfully established model animals were treated by blood-letting puncture at twelve Jing-Well points of the hand, and/or by injecting mannitol into the caudal vein twice daily. Brain tissue was collected at 24, 48 and 72 hours after modeling, and blood was collected through the retinal vein before Evans blue was injected, approximately 1 hour prior to harvesting of brain tissue. Results showed that Evans blue leakage into brain tissue and serum nitric oxide synthase activity were significantly increased in model rats. Treatment with blood-letting punctures at twelve Jing-Well points of the hand and/or injection of mannitol into the caudal vein reduced the amount of Evans blue leakage into the brain tissue and serum nitric oxide synthase activity to varying degrees. There was no significant difference between single treatment and combined treatment. Experimental findings indicate that blood-letting punctures at twelve Jing-Well points of the hand can decrease blood-brain barrier permeability and serum nitric oxide synthase activity in rats following middle cerebral artery occlusion, and its effect is similar to that of mannitol injection alone and Jing-Well points plus mannitol injection.展开更多
目的:观察手十二井穴点刺放血法对大鼠脑缺血超早期脑细胞内游离C a2+浓度(〔C a2+〕i)和细胞外游离C a2+浓度(〔C a2+〕o)的干预作用,探讨其在中风急救中的作用机制。方法:30只W istar大鼠随机分为假手术组、模型组和治疗组。应用针型C...目的:观察手十二井穴点刺放血法对大鼠脑缺血超早期脑细胞内游离C a2+浓度(〔C a2+〕i)和细胞外游离C a2+浓度(〔C a2+〕o)的干预作用,探讨其在中风急救中的作用机制。方法:30只W istar大鼠随机分为假手术组、模型组和治疗组。应用针型C a2+选择电极观测大鼠脑缺血即刻至20 m in期间每分钟脑缺血区皮质细胞〔C a2+〕o的变化。应用Fura 2/AM荧光探针技术观测脑缺血后20 m in脑缺血区皮质神经元突触体内胞浆〔C a2+〕i。结果:与假手术组比较,模型组大鼠造成脑缺血后,缺血区皮质细胞〔C a2+〕o从第5 m in开始出现明显降低(P<0.05),到20 m in为止其下降幅度与时间呈正相关(P<0.01),而缺血区皮质神经元突触体内胞浆〔C a2+〕i显著增高(P<0.01)。与模型组比较,治疗组大鼠脑缺血后缺血区皮质细胞〔C a2+〕o从第8 m in开始其下降幅度明显减小(P均<0.05),到第18 m in此趋势更加显著(P均<0.01),第20 m in缺血区皮质神经元突触体内胞浆〔C a2+〕i显著降低(P<0.01)。结论:脑缺血超早期应用手十二井穴点刺放血法进行干预可快速起效,起到调节缺血区脑细胞内外游离C a2+浓度,有效抑制神经元内钙超载,保护脑细胞功能的作用。展开更多
基金sponsored by the Open Research Fund of Zhejiang First-foremost Key Subject-Acupuncture & Moxibustion,No. ZTK2010A07
文摘A rat model of middle cerebral artery permanent occlusion was established using the modified Longa method. Successfully established model animals were treated by blood-letting puncture at twelve Jing-Well points of the hand, and/or by injecting mannitol into the caudal vein twice daily. Brain tissue was collected at 24, 48 and 72 hours after modeling, and blood was collected through the retinal vein before Evans blue was injected, approximately 1 hour prior to harvesting of brain tissue. Results showed that Evans blue leakage into brain tissue and serum nitric oxide synthase activity were significantly increased in model rats. Treatment with blood-letting punctures at twelve Jing-Well points of the hand and/or injection of mannitol into the caudal vein reduced the amount of Evans blue leakage into the brain tissue and serum nitric oxide synthase activity to varying degrees. There was no significant difference between single treatment and combined treatment. Experimental findings indicate that blood-letting punctures at twelve Jing-Well points of the hand can decrease blood-brain barrier permeability and serum nitric oxide synthase activity in rats following middle cerebral artery occlusion, and its effect is similar to that of mannitol injection alone and Jing-Well points plus mannitol injection.
文摘目的:观察手十二井穴点刺放血法对大鼠脑缺血超早期脑细胞内游离C a2+浓度(〔C a2+〕i)和细胞外游离C a2+浓度(〔C a2+〕o)的干预作用,探讨其在中风急救中的作用机制。方法:30只W istar大鼠随机分为假手术组、模型组和治疗组。应用针型C a2+选择电极观测大鼠脑缺血即刻至20 m in期间每分钟脑缺血区皮质细胞〔C a2+〕o的变化。应用Fura 2/AM荧光探针技术观测脑缺血后20 m in脑缺血区皮质神经元突触体内胞浆〔C a2+〕i。结果:与假手术组比较,模型组大鼠造成脑缺血后,缺血区皮质细胞〔C a2+〕o从第5 m in开始出现明显降低(P<0.05),到20 m in为止其下降幅度与时间呈正相关(P<0.01),而缺血区皮质神经元突触体内胞浆〔C a2+〕i显著增高(P<0.01)。与模型组比较,治疗组大鼠脑缺血后缺血区皮质细胞〔C a2+〕o从第8 m in开始其下降幅度明显减小(P均<0.05),到第18 m in此趋势更加显著(P均<0.01),第20 m in缺血区皮质神经元突触体内胞浆〔C a2+〕i显著降低(P<0.01)。结论:脑缺血超早期应用手十二井穴点刺放血法进行干预可快速起效,起到调节缺血区脑细胞内外游离C a2+浓度,有效抑制神经元内钙超载,保护脑细胞功能的作用。
