Blood-letting punctures at twelve Jing-Well points of the hand can treat cerebral ischemia in a similar manner to mannitol

A rat model of middle cerebral artery permanent occlusion was established using the modified Longa method. Successfully established model animals were treated by blood-letting puncture at twelve Jing-Well points of the hand, and/or by injecting mannitol into the caudal vein twice daily. Brain tissue was collected at 24, 48 and 72 hours after modeling, and blood was collected through the retinal vein before Evans blue was injected, approximately 1 hour prior to harvesting of brain tissue. Results showed that Evans blue leakage into brain tissue and serum nitric oxide synthase activity were significantly increased in model rats. Treatment with blood-letting punctures at twelve Jing-Well points of the hand and/or injection of mannitol into the caudal vein reduced the amount of Evans blue leakage into the brain tissue and serum nitric oxide synthase activity to varying degrees. There was no significant difference between single treatment and combined treatment. Experimental findings indicate that blood-letting punctures at twelve Jing-Well points of the hand can decrease blood-brain barrier permeability and serum nitric oxide synthase activity in rats following middle cerebral artery occlusion, and its effect is similar to that of mannitol injection alone and Jing-Well points plus mannitol injection.

Principle and operation of Jing-well point temperatures' test

Principle and operation of Jing-well point temperatures' test is a method to diagnose and treat diseases by measuring and comparing the symmetrical Jing-well point temperature of human body 12 meridians.It is an important supplement to traditional Chinese medicine four diagnoses. The theoretical basis are that the Jing-well is closely related to Meridians and Viscera, the basic traditional Chinese medicine principle of 'governing exterior to infer interior' and the balancing phenomenon of Meridians. In this paper, the operation steps and treatment measures of Jing-well point temperatures' test are put forward, and 2 successful cases are given. At the end of this paper, the prospect of this method is discussed.

Interpretation of data and treatment of Jing-well point temperatures test

The Jing-well point temperatures test method is a method to diagnose and guide the treatment of diseases by measuring the subjects' symmetrical well point temperature. it is improved from the method of knowing heat sensitivity. The application of Jing-well point temperatures test method is wide, and it can be used in internal and external gynecology and pediatrics and facial features department. at the same time, it has the advantage of objective and accurate diagnosis. The old law has some shortcomings, such as poor intuition, unavoidable omission of information, incomplete interpretation of information and so on. In this paper, Excel software is used to transform the data into line chart form, which improves the intuition and comprehensiveness of this method, so that the data can be better interpreted and used. It is newly proposed in this article that in addition to observing the longitudinal di fference of well point temperature, more attention should be paid to the horizontal contrast difference of well point temperature in different meridians. The article also summarizes a number of treatment methods, including acupuncture, moxa moxibustion, cupping and scraping, and the selection of acupoints, including mother acupoints, tenderness points and heat-sensitive moxibustion, so that doctors can combine traditional Chinese medicine professional knowledge in clinic.

Bloodletting Acupuncture at Jing-Well Points Alleviates Myocardial Injury in Acute Altitude Hypoxic Rats by Activating HIF-1 α/BNIP3 Signaling-Mediated Mitochondrial Autophagyand Decreasing Oxidative Stress

Objective: To explore the protective effect and possible mechanisms of bloodletting acupuncture at Jing-well points(BAJP) pre-treatment on acute hypobaric hypoxia(AHH)-induced myocardium injury rat. Methods:Seventy-five rats were randomly divided into 5 groups by a random number table: a control group(n=15), a model group(n=15), a BAJP group(n=15), a BAJP+3-methyladenine(3-MA) group(n=15), and a BANA(bloodletting at nonacupoint;tail bleeding, n=15) group. Except for the control group, the AHH rat model was established in the other groups, and the corresponding treatment methods were adopted. Enzyme-linked immunosorbent assay(ELISA) was used to detect creatine kinase isoenzyme MB(CK-MB) and cardiac troponins I(CTn I) levels in serum and superoxide dismutase(SOD) and malondialdehyde(MDA) levels in myocardial tissue. Hematoxylin-eosin(HE)staining was used to observe myocardial injury, and terminal deoxynucleotidyltransferase-mediated d UTP-biotin nick end labeling(TUNEL) staining was used to observe cell apoptosis. Transmission electron microscopy detection was used to observe mitochondrial damage and autophagosomes in the myocardium. The mitochondrial membrane potential of the myocardium was analyzed with the fluorescent dye JC-1. Mitochondrial respiratory chain complex(complex Ⅰ, Ⅲ, and Ⅳ) activities and ATPase in the myocardium were detected by mitochondrial respiratory chain complex assay kits. Western blot analysis was used to detect the autophagy index and hypoxia inducible factor-1α(HIF-1α)/Bcl-2 and adenovirus E1B 19k Da-interacting protein 3(BNIP3) signaling. Results:BAJP reduced myocardial injury and inhibited myocardial cell apoptosis in AHH rats. BAJP pretreatment decreased MDA levels and increased SOD levels in AHH rats(all P<0.01). Moreover, BAJP pretreatment increased the mitochondrial membrane potential(P<0.01), mitochondrial respiratory chain complex(complexes Ⅰ, Ⅲ, and Ⅳ)activities(P<0.01), and mitochondrial ATPase activity in AHH rats(P<0.05). The results from electron microscopy demonstrated that BAJP pretreatment improved mitochondrial swelling and increased the autophagosome number in the myocardium of AHH rats. In addition, BAJP pretreatment activated the HIF-1α/BNIP3pathway and autophagy. Finally, the results of using 3-MA to inhibit autophagy in BAJP-treated AHH rats showed that suppression of autophagy attenuated the treatment effects of BAJP in AHH rats, further proving that autophagy constitutes a potential target for BAJP treatment of AHH. Conclusion: BAJP is an effective treatment for AHH-induced myocardial injury, and the mechanism might involve increasing HIF-1α/BNIP3 signaling-mediated autophagy and decreasing oxidative stress.

Bloodletting Acupuncture at Jing-Well Points on Hand Induced Autophagy to Alleviate Brain Injury in Acute Altitude Hypoxic Rats by Activating PINK1/Parkin Pathway

Objective: To explore the protective effect of bloodletting acupuncture at twelve Jing-well points on hand(BAJP) on acute hypobaric hypoxia(AHH)-induced brain injury in rats and its possible mechanisms.Methods: Seventy-five Sprague Dawley rats were divided into 5 groups by a random number table(n=15),including control, model, BAJP, BAJP+3-methyladenine(3-MA), and bloodletting acupuncture at non-acupoint(BANA, tail tip blooding) groups. After 7-day pre-treatment, AHH models were established using hypobaric oxygen chambers. The levels of S100B, glial fibrillary acidic protein(GFAP), superoxide dismutase(SOD), and malondialdehyde(MDA) in serum were measured by enzyme-linked immunosorbent assay. Hematoxylin-eosin staining and the terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling method were used to assess hippocampal histopathology and apoptosis. Transmission electron microscopy assay was used to observe mitochondrial damage and autophagosomes in hippocampal tissues. Flow cytometry was used to detect mitochondrial membrane potential(MMP). The mitochondrial respiratory chain complexes Ⅰ, Ⅲ and Ⅳ activities and ATPase in hippocampal tissue were evaluated, respectively. Western blot analysis was used to detect the protein expressions of Beclin1, autophagy protein 5(ATG5), microtubule-associated protein 1 light chain 3 beta(LC3B), phosphatase and tensin homolog induced kinase 1(PINK1), and Parkin in hippocampal tissues. The mRNA expressions of Beclin1, ATG5 and LC3-Ⅱ were analyzed by quantitative real-time polymerase chain reaction. Results: BAJP treatment reduced hippocampal tissue injury and inhibited hippocampal cell apoptosis in AHH rats. BAJP reduced oxidative stress by decreasing S100B, GFAP and MDA levels and increasing SOD level in the serum of AHH rats(P<0.05 or P<0.01). Then, BAJP increased MMP, the mitochondrial respiratory chain complexes Ⅰ, Ⅲ and Ⅳ activities, and the mitochondrial ATPase activity in AHH rats(all P<0.01). BAJP improved mitochondrial swelling and increased the autophagosome number in hippocampal tissue of AHH rats. Moreover,BAJP treatment increased the protein and mRNA expressions of Beclin1 and ATG5 and LC3-Ⅱ/LC3-Ⅰratio in AHH rats(all P<0.01) and activated the PINK1/Parkin pathway(P<0.01). Finally, 3-MA attenuated the therapeutic effect of BAJP on AHH rats(P<0.05 or P<0.01). Conclusion: BAJP was an effective treatment for AHH-induced brain injury, and the mechanism might be through reducing hippocampal tissue injury via increasing the PINK1/Parkin pathway and enhancement of mitochondrial autophagy.

Wake-Promoting Effect of Bloodletting Puncture at Hand Twelve Jing-Well Points in Acute Stroke Patients:A Multi-center Randomized Controlled Trial

Objective To assess the effect and safety of bloodletting puncture at hand twelve Jing-Well points(HTWPs)in acute stroke patients with conscious disturbance.Methods In this multi-center and randomized controlled trial,360 patients suffered from ischemic or hemorrhagic stroke with conscious disturbance within 48 h from the onset of symptom were divided into bloodletting(180 cases)and control(180 cases)groups using a block randomization.Patients in both groups received routine Western medicine,and patients in the bloodletting group received additional bloodletting puncture at HTWPs on admission immediately before conventional treatment.The primary outcome measure was Glasgow Coma Scale(GCS)score and the secondary outcomes included blood pressure,respiratory rate and pulse rate.All variables were evaluated at baseline(before bloodletting),0(after bloodletting immediately),15,30,50 and 80 min post bloodletting.Results At 80 min post bloodletting,the proportion of patients with improved consciousness in the bloodletting group was greater than the control group(P<0.05).In the separate analysis of moderate consciousness disturbance subgroup,bloodletting therapy benefited ischemic patients,and improved the eye and language response of GCS score at 15,30,50,80 min post bloodletting(P<0.05 or P<0.01).No significant differences were observed regarding the secondary outcomes between two groups(P>0.05).Conclusion The bloodletting puncture at HTWPs was safe and could improve conscious levels of ischemic stroke patients,highlighting a first-aid intervention for acute stroke.

Bloodletting Puncture at Hand Twelve Jing-Well Points Relieves Brain Edema after Severe Traumatic Brain Injury in Rats via Inhibiting MAPK Signaling Pathway

Objective:To investigate whether blood-brain barrier(BBB)served a key role in the edema-relief effect of bloodletting puncture at hand twelve Jing-well points(HTWP)in traumatic brain injury(TBI)and the potential molecular signaling pathways.Methods:Adult male Sprague-Dawley rats were assigned to the shamoperated(sham),TBI,and bloodletting puncture(bloodletting)groups(n=24 per group)using a randomized number table.The TBI model rats were induced by cortical contusion and then bloodletting puncture were performed at HTWP twice a day for 2 days.The neurological function and cerebral edema were evaluated by modified neurological severity score(mNSS),cerebral water content,magnetic resonance imaging and hematoxylin and eosin staining.Cerebral blood flow was measured by laser speckles.The protein levels of aquaporin 4(AQP4),matrix metalloproteinases 9(MMP9)and mitogen-activated protein kinase pathway(MAPK)signaling were detected by immunofluorescence staining and Western blot.Results:Compared with TBI group,bloodletting puncture improved neurological function at 24 and 48 h,alleviated cerebral edema at 48 h,and reduced the permeability of BBB induced by TBI(all P<0.05).The AQP4 and MMP9 which would disrupt the integrity of BBB were downregulated by bloodletting puncture(P<0.05 or P<0.01).In addition,the extracellular signal-regulated kinase(ERK)and p38 signaling pathways were inhibited by bloodletting puncture(P<0.05).Conclusions:Bloodletting puncture at HTWP might play a significant role in protecting BBB through regulating the expressions of MMP9 and AQP4 as well as corresponding regulatory upstream ERK and p38 signaling pathways.Therefore,bloodletting puncture at HTWP may be a promising therapeutic strategy for TBI-induced cerebral edema.

Effects of bloodletting puncture at Jing-Well points in distal ends of finger and toe on survival rate and brain edema in cerebral ischemic rats

OBJECTIVE:To observe the effects of bloodletting puncture at Jing-Well points in the distal ends of the finger and toe on survival rate,survival time,and brain edema in rats with cerebral ischemia.METHODS:Fifty-four male Sprague-Dawley rats were randomly divided into five groups:normal,sham operation,model,bloodletting puncture at Jing-Well points in distal ends of finger and toe,and puncture without bloodletting at these points.Middle cerebral artery occlusion models were established according to Longa's method.The brains were taken 48 h after the model was established.Brain water content,brain density,brain coefficient,survival rate,and survival time in each group were measured.RESULTS:After bloodletting puncture,the survival time of the rats was prolonged,their brain water content and brain coefficient were reduced,and brain density was increased.CONCLUSION:Bloodletting puncture at Jing-Well points in the distal ends of the finger and toe can improve function in ischemic brain edema.

Influences of electro-acupuncture at related Jing-well Points in rats with vascular dementia

OBJECTIVE:To observe the effects of electro-acupuncture(EA) at related Jing-well Points(HT 9,PC 9,KI 1 and LU 11) in rats with vascular dementia(VD) and discuss the relative mechanism.METHODS:A randomized controlled animal experiment was designed.A total of 104 rats were involved in the present study and divided randomly into 4 groups:sham-operation group,model group,Jing-well Points group,and medication group.The VD model was established according to the modified 4-vessel occlusion(4-VO) method.VD rats in the Jing-well Points group were treated by EA at the related Jing-well Points(HT 9,PC 9,KI 1 and LU 11) while those in the medication group were treated with nimotop.The step-down avoidance test was performed before and after treatment in all rats.Latency and error frequency indexed memory function were recorded.Nitric oxide(NO) levels and superoxide dismutase(SOD) activity in both cerebral tissue and serum were detected after the treatment course.RESULTS:A total of 42 rats were included in the final analysis.Compared with the model group,the latency in the Jing-well Points group was significantly prolonged(P<0.01) and the error frequency was significantly decreased(P<0.05) after therapy;the decrease in NO levels in both brain tissue and serum was significant(P<0.05 and P<0.01,respectively);and the increase in SOD activity was also significant(P<0.01).There was no significant difference in latency,error frequency,NO levels and SOD activity between the Jing-well Points group and medication group.CONCLUSION:EA at related Jing-well Points can remarkably improve memory impairment in VD rats.Moreover,decreasing the overproduction of NO and strengthening the ability of eliminating free radicals may provide therapeutic potential for the treatment of VD.

Analysis of Similarities and Differences of the Twelve Jing-Well Acupoints for Emergency

The twelve Jing-Well acupoints are located at the tips of the limbs,with strong stimulation andintense needling sensation,and play important roles in the treatment of various critical diseases.The twelve Jing-Well acupoints share similarities and have specificities in the treatment of critical and severe diseases.The similarities of the twelve Jing-Well acupoints are supposed to open the orifices and remove blockage,clear away heat,resolve swelling,dissolve toxin and remove blood stasis.The Jing-Well acupoints locate at the different positions also have their specific effects for emergencies.Shaoshang(LU 11)is supposed to clean and benefit the throat.Shangyang(LI 1)is supposed to clear away heat and purge Fu organs.Lidui(ST 45)is supposed to expel fire and stop fear.Yinbai(SP 1)is supposed to stop bleeding and correct collapse.Shaochong(HT 9)is supposed to clear away heat from the heart and get rid of vexation.Shaoze(SI 1)is supposed to dredge the breast collateral.Zhiyin(BL 67)is supposed to promote birth and smoothen delivery.Yongquan(KI 1)is supposed to nourish yin and suppress yang.Zhongchong(PC 9)is supposed to benefit the orifice and brighten the eyes.Guanchong(TE 1)is supposed to clean and promote the triple energizer.Zuqiaoyin(GB 44)is supposed to induce menstruation and stop pain.Dadun(LR 1)is supposed to clear away the liver fire and relieve hernia.It is advisable to identify the similarities and differences and apply different stimulating modes in order to guide the clinical practice and enhance the emergency effect.

手十二井穴刺络放血疗法中枢响应特征的静息态fMRI研究方案

目的:探讨手十二井穴刺络放血疗法的中枢响应特征。方法:纳入30例健康志愿者作为受试对象,完成30 min基线静息态fMRI数据采集后,即刻行手十二井穴刺络放血操作,控制在5 min内。刺络放血后,立即行fMRI数据采集,参数同基线静息态扫描序列。预期结果:与基线相比,手十二井穴刺络放血后,静息态脑功能显著变化,其中脑干网状上行激活系统醒觉相关脑区变化尤为显著。

井穴在炎症性疾病中的应用概述

井穴为十二经脉阴阳之气始发与交接之处,古人常取其以醒神清热。现代研究多关注井穴在急性中枢神经损伤后的昏迷急救效果,对于其他作用缺乏清晰的认识。通过分析井穴的相关理论和古代医案,发现井穴具有良好的抗炎作用,认为井穴所清之热多为对炎症性疾病的治疗。临床中,井穴疗法在炎症性疾病的治疗中应用广泛,能有效改善中枢神经损伤的炎性反应和外周头面五官、呼吸系统、关节软组织处的急慢性炎症,具有简便易行、安全高效、不良反应少的特点,适于在临床中推广应用。参考文献46篇。

基于PI3K/AKT/mTOR信号通路探讨井穴放血对急性高原低氧脑损伤的保护作用

目的探讨井穴放血通过调控PI3K/AKT/mTOR信号通路介导的线粒体自噬对急性高原低氧脑损伤的保护作用及分子机制,为临床使用井穴放血防治急性高原低氧脑损伤提供有效靶点及理论依据。方法雄性SD大鼠随机分为对照组(Control组)和实验组,实验组分为模型组(Model组)、井穴放血组(Bloodletting acupuncture at jing-well points组,BAJP组)、非穴位放血组(Bloodletting acupuncture at nonacupoint组,BANA组),每组15只。低压氧舱减压至6000 m海拔高度,将各实验组大鼠低压低氧处理72 h复制急性高原低氧脑损伤大鼠模型。入舱前7天,BAJP组大鼠按照少商、商阳、中冲、关冲、少冲、少泽的顺序点刺放血,每天1次;BANA组大鼠每日剪尾尖放血,两组放血量均为15-20μL。HE染色观察脑组织形态结构变化;透射电镜观察大鼠神经元细胞内线粒体损伤及自噬体形成情况;TUNEL观察海马组织细胞凋亡情况;Western blot检测PI3K、AKT、mTOR表达水平;PCR检测AKT、mTOR mRNA表达水平。结果①与Control组相比,急性高原低氧(Acute high-altitude hypoxia,AHH)大鼠海马组织CA1区变性坏死椎体细胞数量明显增多、线粒体肿胀、自噬体出现、海马组织细胞凋亡数量增加;经过井穴放血治疗后,AHH大鼠各种脑损伤表现均得到缓解;非穴位放血对AHH大鼠脑损伤无明显改善作用。②Western blot检测AHH大鼠海马组织内PI3K、AKT、mTOR磷酸化水平相较于Control组大鼠明显下降(P<0.01),经过井穴放血治疗后,3个分子磷酸化水平进一步下降(P<0.01),非穴位放血治疗未对这些分子的磷酸化水平产生显著影响(P>0.05),AKT、mTOR mRNA表达水平进一步证明了上述趋势。结论井穴放血可以对急性高原低氧脑损伤起到保护作用,具有穴位特殊性,其机制可能与抑制PI3K/AKT/mTOR通路促进线粒体自噬水平升高,改善线粒体生理,增强机体抗细胞凋亡、耐缺氧能力有关。

从“井穴刺络放血”论治突发性耳聋

突发性耳聋为临床常见难治性病证,发病率连年上升。中医认为其发病多与气血不畅、经络阻塞、耳窍不通相关。井穴作为经气之源,与脏腑经络气血密切相关,有疏经通络、调和气血之效。井穴刺络放血具有改善耳部血液微循环,促进内耳神经恢复的作用。探析井穴与耳之间的联系,为井穴刺络放血治疗“突聋”提供理论依据。

“十二井穴”针法对局灶性脑缺血大鼠脑皮质及血清肿瘤坏死因子-α含量的影响

目的:探讨“十二井穴”针法对脑缺血损伤后大鼠肿瘤坏死因子TNF-α影响的时效性。方法:雄性Wistar大鼠96只,随机分为正常组、假手术组各8只,脑缺血模型组、针刺治疗组各40只。后两组又分为1、3、6、12、24 h各时段组,每时间段组各8只。采用光化学诱导法制作一侧大脑局灶性脑缺血模型,施以“十二井穴”针法,在造模后1、3、6、122、4 h不同时间段开始进行针刺治疗,治疗结束后处死大鼠。运用放射免疫分析法测定各组动物脑皮质及血清中TNF-α含量。结果:局灶性脑缺血后1 h,脑皮质及血清TNF-α即明显升高,至24 h仍明显高于正常组,P<0.05;针刺后各组动物脑皮质及血清中TNF-α含量与同时段模型组比较明显降低,P<0.05,0.01。结论:针刺“十二井穴”有降低脑缺血大鼠脑组织及血清TNF-α的作用,这可能是“十二井穴”针法治疗缺血性脑血管疾病特别是早期介入治疗的机理之一。

大接经法治疗血管性痴呆临床观察

目的 :探讨治疗血管性痴呆的临床有效方法。方法 :将 96例血管性痴呆患者随机分为观察组、头针组、药物组 ,每组 32例 ,进行相关治疗。观察治疗前后各组临床疗效、长谷川痴呆修改量表 (HDS R)评分、精神认知能力、日常生活能力及神经功能缺损评分 ,主要症状变化。 结果 :3组治疗后均有一定疗效 ,但以观察组疗效最好 ,差异有非常显著性意义 (P <0 0 1)。结论 :大接经法治疗血管性痴呆疗效确切 ,可改善主要临床症状 ,促进智能、生活自理能力的提高。

十二井穴刺络放血联合亚低温治疗对颅脑创伤急性期大鼠脑水肿的影响

目的:探讨十二井穴刺络放血联合亚低温对颅脑创伤(TBI)大鼠急性期脑水肿的影响。方法:将75只健康成年雄性SD大鼠随机分为5组(n=15):假手术组(Sham)、颅脑创伤组(TBI)、放血组(BL)、亚低温组(MIH)、放血联合亚低温组(BL+MIH)。采用电子脑皮质损伤撞击仪(eCCI)建立大鼠TBI模型,BL组于伤后即刻行十二井穴刺络放血,每日2次;MIH组在伤后即刻采用亚低温冰毯使体温降至32℃,持续干预6 h。伤后48 h分别采用核磁共振成像技术(MRI)观察脑水肿变化(n=3)、神经功能缺损评分(m NSS)观察行为学改变及干/湿重法测定脑含水量(n=8)、伊文思蓝染色(EB)检测血脑屏障通透性(BBB)(n=4)。结果:MRI显示,TBI组脑水肿及血肿明显,中线明显偏移;而干预组较TBI组水肿明显减轻,中线居中。与TBI组比较,各干预组m NSS评分均明显改善(P<0.05),而且BL+MIH组优于单独BL和MIH组(均P<0.01);各干预组脑含水量也有不同程度降低(P<0.05),尤以MIH组和BL+MIH组降低最为显著(P<0.01);各干预组血脑屏障通透性均有明显改善(均P<0.01),而且MIH组和BL+MIH组显著优于单独BL组(P<0.05,P<0.01)。结论:十二井穴刺络放血和亚低温均可以降低神经功能缺陷评分,减轻脑水肿,降低血脑屏障通透性,对创伤性大鼠脑组织有保护作用,且二者联合治疗效果更加明显。

井穴放血疗法对卒中后抑郁患者抑郁状况和血清5-羟色胺水平的影响研究

目的探讨井穴放血疗法对卒中后抑郁(PSD)患者抑郁状况和血清5-羟色胺(5-HT)水平的影响。方法选取于2012年6月—2013年12月在贵阳中医学院第二附属医院康复科进行治疗的PSD患者80例,随机分为对照组和观察组各40例。两组患者均进行常规内科治疗和肢体康复治疗,对照组在常规治疗基础上口服帕罗西汀,观察组在常规治疗基础上加用井穴放血疗法,均治疗2个疗程。比较两组治疗前后的汉密尔顿抑郁量表(HAMD)评分和血清5-HT水平。结果两组性别、平均年龄、平均病程及卒中类型分布比较,差异无统计学意义(P>0.05)。治疗前两组血清5-HT水平比较,差异无统计学意义(P>0.05);治疗后两组HAMD评分比较,差异亦无统计学意义(P>0.05);治疗后两组HAMD评分与治疗前比较,差异有统计学意义(P<0.05)。治疗前两组血清5-HT水平比较,差异无统计学意义(P>0.05);治疗后两组血清5-HT水平比较,差异亦无统计学意义(P>0.05);治疗后两组血清5-HT水平与治疗前比较,差异有统计学意义(P<0.05)。结论井穴放血疗法可以改善PSD患者的抑郁状况,其机制可能与降低血清5-HT水平有关。

十二井穴刺络放血联合薏苡仁对颅脑创伤性脑水肿作用的研究进展

十二井穴刺络放血和中药薏苡仁已被广泛应用于临床治疗,两者单独应用也取得不同程度的临床疗效,但对于他们联合应用治疗颅脑损伤的机制及治疗效果的研究报道少之又少。文章查阅了大量文献,总结出十二井穴刺络放血对颅脑创伤性昏迷患者的醒脑开窍作用和通过调节氢离子(H^+)、钾离子(K^+)、钠离子(Na^+)、钙离子(Ca^(2+))等离子而达到减轻脑水肿及神经保护作用的中西医理论依据,并且阐述了薏苡仁治疗创伤性脑水肿的研究进展,以及两者联合应用对颅脑创伤性脑水肿的疗效。

针刺井穴治疗血管性痴呆的特异性研究

目的选用不同井穴治疗血管性痴呆(VD),观察其疗效及差异。方法选取合格受试患者140例,按入院顺序,随机纳入井穴组、药物组和非经穴组,并给予相应处理,针刺治疗每次留针30 min,留针期间每10 min行针1次,每日治疗1次,连续10次为1个疗程,共治疗2个疗程;药物选用茴拉西坦0.2 g/次,口服,每日3次,连服20 d。治疗前后均接受MMSE、ADL量表积分评定及TCD检查。结果治疗后十二井穴各组在改善MMSE、ADL与脑血管平均血流速度上均优于非经穴组(P<0.05)。与药物组比较,在改善MMSE评分疗效上商阳组、少冲组、中冲组、至阴组、大敦组有统计学差异(P<0.05);在改善ADL评分效果上,少冲组、中冲组与其他井穴组比较效果更明显(P<0.05);在改善各条脑血管平均流速效果上,少冲组、中冲组及大敦组均有7条以上脑血管平均流速改善幅度大于药物组。结论不同井穴治疗VD效果有一定的差异性,手少阴心经井穴少冲穴和手厥阴心包经井穴中冲穴疗效较为优越。