A Dietary Supplement Jinghuosu Ameliorates Reproductive Damage Induced by Tripterygium Glycosides

Objective To determine the possible protective effects of Jinghuosu,a dietary supplement(DS),on tripterygium glycosides(TG)-induced reproductive system injury in rats and its underlying mechanisms.Methods A reproductive damage model was established in rats by feeding of TGs.Twenty-eight male Sprague Dawley rats were randomly divided into 4 groups using a random number table(n=7 in each):control(C)group,model(M)group,DS group and L-carnitine(LC)group.Rats in M,DS and LC groups received 40 mg/kg TGs orally.Starting from the 5th week,after administration of TGs for 4 h every day,rats in DS and LC groups were administered with 2.7 g/kg DS and 0.21 g/kg LC,respectively,for protective treatment over the next 4 weeks.Rats in Group C continued to receive the control treatment.Hematoxylin-eosin staining was used for histopathological analysis of rat testicular tissues.Enzyme-linked immunosorbent assay was performed to measure alkaline phosphatase(ALP),lactate dehydrogenase,alcohol dehydrogenase,total antioxidant capacity(T-AOC),superoxide dismutase,glutathione peroxidase(GSH-Px),and malondialdehyde(MDA)concentrations.Chemiluminescence assay was used to determine the serum testosterone content.Quantitative real-time PCR and Western blotting were conducted to analyze the expression of genes and proteins related to the testosterone synthesis pathway and the nuclear factor erythroid 2-related factor 2/heme oxygenase 1 antioxidant pathway.Results Oral administration of TGs induced significant increases in the testicular levels of zinc transporter 1 and MDA(P<0.05).On the other hand,sperm concentration,sperm motility,and serum testosterone,serum zinc,testicular zinc,Zrt-,Irt-like protein 1,ALP,luteinizing hormone(LH)receptor,steroidogenic acute regulatory protein,Cytochrome P450 family 11 subfamily A member 1,3β-hydroxysteroid dehydrogenase 1 T-AOC,GSH-Px,nuclear factor erythroid 2-related factor 2,heme oxygenase-1 and NAD(P)H:quinone oxidoreductase 1 levels decreased following TGs exposure(P<0.05).All of these phenotypes were evidently reversed by DS(P<0.05).Conclusion DS Jinghuosu protects against TG-induced reproductive system injury in rats,probably by improving zinc homeostasis,enhancing the testosterone synthesis and attenuating oxidative stress.

精活速治疗少弱精子症疗效观察

目的:探讨精活速治疗少弱精子症的有效性。方法:120例特发性轻中度少、弱精子症患者入选本研究;口服精活速,1袋/次,2次/d,连续3个月;分别于治疗前及治疗后第1、2、3个月末,采用计算机辅助精液分析检测治疗前后每次射精后精液的各项参数变化(精子浓度、精子总活力、前向运动精子百分率、正常形态精子百分率),每次随访均记录患者有无明显不良反应。结果:治疗3个月后,入组患者的精液质量有了较明显的改善。精液中精子浓度、精子总活动率、前向运动精子百分率和精子形态正常百分率,在服用精活速治疗3个月后均有了较明显的改善(P<0.05),观察的3个月期间内,未见明显不良反应。结论:精活速治疗轻中度少弱精子症患者症疗效显著,无明显不良反应。

二仙汤加减联合精活速治疗肾阳亏虚型少弱精子症的临床观察

目的观察二仙汤加减联合精活速治疗肾阳亏虚型少弱精子症的临床疗效。方法将105例肾阳亏虚型少弱精子症患者随机分为联合组、中药组及精活速组,每组35例。联合组予二仙汤加减联合精活速治疗,中药组以二仙汤加减治疗,精活速组予精活速治疗。各组疗程均为3个月,观察临床疗效,比较精液常规指标的变化情况。结果 (1)精活速组、中药组、联合组临床总有效率分别为62.9%、65.7%、82.9%。组间临床疗效比较,联合组明显优于精活速组(P<0.05)、亦优于中药组(P<0.05);精活速组与中药组疗效差异无统计学意义(P>0.05)。(2)治疗前后组内比较,各组精液浓度、精子成活率、a级精子比例、(a+b)级精子比例差异均有统计学意义(P<0.05)。组间治疗后,中药组与精活速组比较,精液浓度及精子成活率差异有统计学意义(P<0.05),联合组对各项指标的改善明显优于精活速组(P<0.05);联合组对各项指标的改善明显优于中药组(P<0.05)。结论二仙汤加减联合精活速治疗肾阳亏虚型少弱精子症,可显著改善患者精液质量,提高精子活力。