Objective:To investigate the expression changes and clinical significance of Toll-like receptor-4(TLR-4)and interleukin-1β(IL-1β)in the serum and knee joint fluid in patients with knee osteoarthritis(KOA).Methods:Fr...Objective:To investigate the expression changes and clinical significance of Toll-like receptor-4(TLR-4)and interleukin-1β(IL-1β)in the serum and knee joint fluid in patients with knee osteoarthritis(KOA).Methods:From June 2017 to December 2018,220 cases of patients with KOA who were admitted in Department of Orthopedics of the Third Affiliated Hospital of Inner Mongolia Medical University(“our hospital”for short)were selected as the observation group.According to the severity of KOA,they were divided into the mild subgroup(n=98),the moderate subgroup(n=80)and the severe subgroup(n=42).In addition,60 cases of volunteers who received the physical examination in Health Medical Center of our hospital were selected as the control group.The levels of TLR-4 and IL-1βin the serum and knee joint fluid were detected by enzyme-linked immunosorbent assay,in order to analyze the correlation of TLR-4 and IL-1βlevels with the severity of KOA.Results:The levels of TLR-4 and IL-1βin the serum and knee joint fluid of the observation group were significantly higher than those of the control group,and the differences were statistically significant(all p<.05).The levels of TLR-4 and IL-1βin the serum and knee joint fluid of the severe subgroup were significantly higher than those of the mild subgroup and the moderate subgroup,and the levels of TLR-4 and IL-1βin the serum and knee joint fluid of the moderate subgroup were higher than those of the mild group.The differences were statistically significant(all p<.05).The levels of TLR-4 and IL-1βin the serum and knee joint fluid were positively correlated to the severity of KOA(r=.706,.729,.741,.715,all p<.05);in the serum,the level of TLR-4 was positively correlated to the level of IL-1β(r=.720,p<.05);in the joint fluid,the level of TLR-4 was positively correlated to the level of IL-1β(r=.736,p<.05).Conclusions:The levels of TLR-4 and IL-1βin the serum and knee joint fluid of KOA patients are abnormally increased,which is closely related with the severity of KOA.It can be used for clinical prediction,diagnosis and treatment of KOA.展开更多
Objective: This study aims to show that a proprietary topical cream can deliver glucosamine through the skin into the synovial fluid of osteoarthritic patients. This cream contains 10% w/w glucosamine sulfate. It also...Objective: This study aims to show that a proprietary topical cream can deliver glucosamine through the skin into the synovial fluid of osteoarthritic patients. This cream contains 10% w/w glucosamine sulfate. It also aims to determine the endogenous level of glucosamine in the synovial fluid of these patients. Therapeutic effectiveness of glucosamine is not addressed in this study. Design: This phase IV, open-label, nonrandomized study enrolled 240 patients. Participants from the Test group received a single dose treatment (2 g of cream), and synovial fluid samples were collected 1 - 3 hours post-treatment. Patients from the Control group were not subjected to any treatment but their synovial fluid was also sampled to establish a glucosamine concentration baseline for Time-0 (T0). Glucosamine concentrations were determined by HPLC analysis. Results: The mean glucosamine concentration in the synovial fluid of patients from the Test group (100.56 ng/ml, 95% CI 66.36 - 134.76, n = 117) was higher than in the Control group (17.83 ng/ml, 95% CI 7.42 - 28.24, n = 117) resulting in a significant between-group difference (p Conclusion: The results suggest that glucosamine can be topically delivered across the human skin into the synovial fluid using a proper vehicle. This suggests that other water-soluble molecules could similarly be delivered transdermally, alleviating the need for oral delivery in cases where oral administration is difficult, or when harmful side effects could ensue.展开更多
In this paper,authors have discussed predominately unidirectional Fluid Structure Interaction,i. e. a given field in which a high speed/pressure and high temperature thermal flow affect the interface between pipelines...In this paper,authors have discussed predominately unidirectional Fluid Structure Interaction,i. e. a given field in which a high speed/pressure and high temperature thermal flow affect the interface between pipelines joints. Surface forces at the fluid-structure interface allow designers to investigate the effects of fluid flow on the structural deformation and stresses. Possible failure modes have been compared with different loads from steady thermal flow analysis results. CFD code SC/Tetra and FEA code ANSYS are used in this study. These studies can be used in protecting certain fatigue failures for pipeline joints under critical cyclic load conditions from both thermal expansion and hydraulic pressure in municipal and environmental engineering applications as well as oil and gas fields.展开更多
轮毂电驱动技术的研究是未来新能源驱动体系研究的重要方向。随着轮毂电驱动对转速的要求越来越高,搅油功率损失成为不可忽略的部分,甚至高达功率总损失的50%~80%。现有的计算搅油损失的方法主要是采用简单的经验公式,无法适用于复杂的...轮毂电驱动技术的研究是未来新能源驱动体系研究的重要方向。随着轮毂电驱动对转速的要求越来越高,搅油功率损失成为不可忽略的部分,甚至高达功率总损失的50%~80%。现有的计算搅油损失的方法主要是采用简单的经验公式,无法适用于复杂的行星齿轮传动。为此,采用计算流体力学(Computational Fluid Dynamics,CFD)软件与C语言用户自定义函数(User Defined Function,UDF)对两级行星齿轮传动飞溅润滑进行联合仿真,实现了油-气两相瞬态流场可视化;通过提取表面的压力和黏性力,得到了太阳轮、行星轮及行星架的搅油损失;对25种工况进行仿真与分析,得到了搅油功率损失随转速和浸油深度的变化趋势。结果表明,搅油功率损失随转速和浸油深度的增加而增大,且无明显的拐点,实现最小搅油损失应当在保证充分润滑的前提下取最小的浸油深度。展开更多
文摘Objective:To investigate the expression changes and clinical significance of Toll-like receptor-4(TLR-4)and interleukin-1β(IL-1β)in the serum and knee joint fluid in patients with knee osteoarthritis(KOA).Methods:From June 2017 to December 2018,220 cases of patients with KOA who were admitted in Department of Orthopedics of the Third Affiliated Hospital of Inner Mongolia Medical University(“our hospital”for short)were selected as the observation group.According to the severity of KOA,they were divided into the mild subgroup(n=98),the moderate subgroup(n=80)and the severe subgroup(n=42).In addition,60 cases of volunteers who received the physical examination in Health Medical Center of our hospital were selected as the control group.The levels of TLR-4 and IL-1βin the serum and knee joint fluid were detected by enzyme-linked immunosorbent assay,in order to analyze the correlation of TLR-4 and IL-1βlevels with the severity of KOA.Results:The levels of TLR-4 and IL-1βin the serum and knee joint fluid of the observation group were significantly higher than those of the control group,and the differences were statistically significant(all p<.05).The levels of TLR-4 and IL-1βin the serum and knee joint fluid of the severe subgroup were significantly higher than those of the mild subgroup and the moderate subgroup,and the levels of TLR-4 and IL-1βin the serum and knee joint fluid of the moderate subgroup were higher than those of the mild group.The differences were statistically significant(all p<.05).The levels of TLR-4 and IL-1βin the serum and knee joint fluid were positively correlated to the severity of KOA(r=.706,.729,.741,.715,all p<.05);in the serum,the level of TLR-4 was positively correlated to the level of IL-1β(r=.720,p<.05);in the joint fluid,the level of TLR-4 was positively correlated to the level of IL-1β(r=.736,p<.05).Conclusions:The levels of TLR-4 and IL-1βin the serum and knee joint fluid of KOA patients are abnormally increased,which is closely related with the severity of KOA.It can be used for clinical prediction,diagnosis and treatment of KOA.
文摘Objective: This study aims to show that a proprietary topical cream can deliver glucosamine through the skin into the synovial fluid of osteoarthritic patients. This cream contains 10% w/w glucosamine sulfate. It also aims to determine the endogenous level of glucosamine in the synovial fluid of these patients. Therapeutic effectiveness of glucosamine is not addressed in this study. Design: This phase IV, open-label, nonrandomized study enrolled 240 patients. Participants from the Test group received a single dose treatment (2 g of cream), and synovial fluid samples were collected 1 - 3 hours post-treatment. Patients from the Control group were not subjected to any treatment but their synovial fluid was also sampled to establish a glucosamine concentration baseline for Time-0 (T0). Glucosamine concentrations were determined by HPLC analysis. Results: The mean glucosamine concentration in the synovial fluid of patients from the Test group (100.56 ng/ml, 95% CI 66.36 - 134.76, n = 117) was higher than in the Control group (17.83 ng/ml, 95% CI 7.42 - 28.24, n = 117) resulting in a significant between-group difference (p Conclusion: The results suggest that glucosamine can be topically delivered across the human skin into the synovial fluid using a proper vehicle. This suggests that other water-soluble molecules could similarly be delivered transdermally, alleviating the need for oral delivery in cases where oral administration is difficult, or when harmful side effects could ensue.
文摘In this paper,authors have discussed predominately unidirectional Fluid Structure Interaction,i. e. a given field in which a high speed/pressure and high temperature thermal flow affect the interface between pipelines joints. Surface forces at the fluid-structure interface allow designers to investigate the effects of fluid flow on the structural deformation and stresses. Possible failure modes have been compared with different loads from steady thermal flow analysis results. CFD code SC/Tetra and FEA code ANSYS are used in this study. These studies can be used in protecting certain fatigue failures for pipeline joints under critical cyclic load conditions from both thermal expansion and hydraulic pressure in municipal and environmental engineering applications as well as oil and gas fields.
文摘轮毂电驱动技术的研究是未来新能源驱动体系研究的重要方向。随着轮毂电驱动对转速的要求越来越高,搅油功率损失成为不可忽略的部分,甚至高达功率总损失的50%~80%。现有的计算搅油损失的方法主要是采用简单的经验公式,无法适用于复杂的行星齿轮传动。为此,采用计算流体力学(Computational Fluid Dynamics,CFD)软件与C语言用户自定义函数(User Defined Function,UDF)对两级行星齿轮传动飞溅润滑进行联合仿真,实现了油-气两相瞬态流场可视化;通过提取表面的压力和黏性力,得到了太阳轮、行星轮及行星架的搅油损失;对25种工况进行仿真与分析,得到了搅油功率损失随转速和浸油深度的变化趋势。结果表明,搅油功率损失随转速和浸油深度的增加而增大,且无明显的拐点,实现最小搅油损失应当在保证充分润滑的前提下取最小的浸油深度。
