Two new cyclic diarylheptanoids juglanin A （1） and juglanin B （2） were isolated from the pericarps of Juglans regia L. Their structures were elucidated by various spectroscopic methods including 2D NMR techniques ...Two new cyclic diarylheptanoids juglanin A （1） and juglanin B （2） were isolated from the pericarps of Juglans regia L. Their structures were elucidated by various spectroscopic methods including 2D NMR techniques （COSY, HMQC, HMBC, NOESY） and HR-ESIMS.展开更多
[Objectives] This study was conducted to explore the fertilization ratio of walnut, so as to provide a basis for rational fertilization of walnut. [Methods] Under the condition of gravelly yellow soil in Chaotian Dist...[Objectives] This study was conducted to explore the fertilization ratio of walnut, so as to provide a basis for rational fertilization of walnut. [Methods] Under the condition of gravelly yellow soil in Chaotian District, Guangyuan City, the orthogonal design of L_9(3~4) was used to test the fertilization ratio of nitrogen, phosphorus and potassium fertilizers. [Results] The effects of nitrogen, phosphorus and potassium fertilizers on walnut yield ranked as phosphate fertilizer>potassium fertilizer>nitrogen fertilizer. The different treatments had great effects on the yield of walnut, and for the land with gravelly yellow soil, the suitable annual fertilization amount of walnut was urea 1 200 g/plant + calcium superphosphate 1 500 g/plant + potassium sulfate 940 g/plant. [Conclusions] This study provides a theoretical basis for rational fertilization of walnut in Sichuan Province.展开更多
Thirteen microsatellite markers were isolated and characterized from Juglans regia L. Successful polymorphism of the 13 primer pairs was observed in various genotypes of J. regia. The number of polymorphic alleles ran...Thirteen microsatellite markers were isolated and characterized from Juglans regia L. Successful polymorphism of the 13 primer pairs was observed in various genotypes of J. regia. The number of polymorphic alleles ranged from 2 to 4 (with an average of 4.35). The polymorphic information content values ranged from 0.47 to 0.88 (with an average of 0.69). TC/AG and GAA/CTT class of repeats were the most abundant di-nucleotide and tri-nucleotide repeats, respectively.展开更多
As a member of nuts, walnut is consumed from snacks to salads and desserts to entrees and an importantpart of human diet for centuries. Walnut biological and nutritional value is also enhanced by its valuable protein ...As a member of nuts, walnut is consumed from snacks to salads and desserts to entrees and an importantpart of human diet for centuries. Walnut biological and nutritional value is also enhanced by its valuable protein and rich in nutrient composition such as vitamins and minerals. The most important characteristic of walnut oil is the abundance of polyunsaturated fatty acids, which makes it a unique food because of high amount of linoleic acid. Due to having valuable protein, vitamins and minerals it enhances biological and nourishment value, also. Recent epidemiological studies showed that consumption of walnut reduce cardiovascular diseases due to the rich in antioxidant properties, valuable fatty acids and tochopherols contents. In Turkey, walnut production and consumption increases year by year. The kernel of walnut genotypes shows variability in terms of their fat, fatty acid and tocopherol profiles. In this paper, it was aimed to characterize 10 walnut (Juglans regia L.) cultivars (Bilecik, Chandler, Hartley, Howard, Maras 12, Maras 18, Midland, Pedro, Sen and Serr) based on their fatty acid profiles using GC (Gas Chromatography), tocopherol and its isomers by HPLC (High Performance Liquid Chromatography) and total phenol content with spectrometric methods. Among the walnut cultivars “Hartley” was the highest linoleic acid (64.56%) and “Howard” was the α-linolenic acid 13.26 (%). The highest values of α (38.76 μg/g), β + γ (312.19 μg/g) and δ-tocopherol (40.77 μg/g) and total phenol (349 mg GAE/100 g ext) content were detected in “Sen” cultivar. Obtained results might be significant for further breeding programme to improve rich in especially γ-tocopherol linoleic acids and total phenolic compounds.展开更多
基于网络药理学方法及分子对接技术分析核桃(Juglans regia L.)治疗高尿酸血症(Hyperuricemia,HUA)的作用机制,利用中药系统药理数据库和分析平台(Traditional Chinese medicine systems pharmacology database and analysis platform,T...基于网络药理学方法及分子对接技术分析核桃(Juglans regia L.)治疗高尿酸血症(Hyperuricemia,HUA)的作用机制,利用中药系统药理数据库和分析平台(Traditional Chinese medicine systems pharmacology database and analysis platform,TCMSP)预测核桃活性成分的作用靶点,运用OMIM、GeneCards和DrugBank数据库检索HUA疾病靶点。使用Cytoscape 3.7.2软件进行作用靶点-活性成分-疾病网络绘制,利用Metascape平台进行富集分析。使用AutoDockTools 1.5.6软件进行分子对接,对活性成分及重要靶点的结合度进行验证。结果表明,共筛选出核桃活性成分5种,与HUA有交集靶点16个;富集程度较高的信号通路包括癌症信号通路、化学致癌-受体激活信号通路、糖尿病并发症中的晚期糖基化产物-晚期糖基化终末产物受体信号通路和卡波西肉瘤相关疱疹病毒信号通路;通过分子对接验证试验可知,核桃中主要活性成分与重要靶点结合紧密。展开更多
文摘Two new cyclic diarylheptanoids juglanin A （1） and juglanin B （2） were isolated from the pericarps of Juglans regia L. Their structures were elucidated by various spectroscopic methods including 2D NMR techniques （COSY, HMQC, HMBC, NOESY） and HR-ESIMS.
基金Supported by Technological Achievements Transformation Fund for Research Institutes in Sichuan Province(14010112)
文摘[Objectives] This study was conducted to explore the fertilization ratio of walnut, so as to provide a basis for rational fertilization of walnut. [Methods] Under the condition of gravelly yellow soil in Chaotian District, Guangyuan City, the orthogonal design of L_9(3~4) was used to test the fertilization ratio of nitrogen, phosphorus and potassium fertilizers. [Results] The effects of nitrogen, phosphorus and potassium fertilizers on walnut yield ranked as phosphate fertilizer>potassium fertilizer>nitrogen fertilizer. The different treatments had great effects on the yield of walnut, and for the land with gravelly yellow soil, the suitable annual fertilization amount of walnut was urea 1 200 g/plant + calcium superphosphate 1 500 g/plant + potassium sulfate 940 g/plant. [Conclusions] This study provides a theoretical basis for rational fertilization of walnut in Sichuan Province.
文摘Thirteen microsatellite markers were isolated and characterized from Juglans regia L. Successful polymorphism of the 13 primer pairs was observed in various genotypes of J. regia. The number of polymorphic alleles ranged from 2 to 4 (with an average of 4.35). The polymorphic information content values ranged from 0.47 to 0.88 (with an average of 0.69). TC/AG and GAA/CTT class of repeats were the most abundant di-nucleotide and tri-nucleotide repeats, respectively.
文摘As a member of nuts, walnut is consumed from snacks to salads and desserts to entrees and an importantpart of human diet for centuries. Walnut biological and nutritional value is also enhanced by its valuable protein and rich in nutrient composition such as vitamins and minerals. The most important characteristic of walnut oil is the abundance of polyunsaturated fatty acids, which makes it a unique food because of high amount of linoleic acid. Due to having valuable protein, vitamins and minerals it enhances biological and nourishment value, also. Recent epidemiological studies showed that consumption of walnut reduce cardiovascular diseases due to the rich in antioxidant properties, valuable fatty acids and tochopherols contents. In Turkey, walnut production and consumption increases year by year. The kernel of walnut genotypes shows variability in terms of their fat, fatty acid and tocopherol profiles. In this paper, it was aimed to characterize 10 walnut (Juglans regia L.) cultivars (Bilecik, Chandler, Hartley, Howard, Maras 12, Maras 18, Midland, Pedro, Sen and Serr) based on their fatty acid profiles using GC (Gas Chromatography), tocopherol and its isomers by HPLC (High Performance Liquid Chromatography) and total phenol content with spectrometric methods. Among the walnut cultivars “Hartley” was the highest linoleic acid (64.56%) and “Howard” was the α-linolenic acid 13.26 (%). The highest values of α (38.76 μg/g), β + γ (312.19 μg/g) and δ-tocopherol (40.77 μg/g) and total phenol (349 mg GAE/100 g ext) content were detected in “Sen” cultivar. Obtained results might be significant for further breeding programme to improve rich in especially γ-tocopherol linoleic acids and total phenolic compounds.
文摘基于网络药理学方法及分子对接技术分析核桃(Juglans regia L.)治疗高尿酸血症(Hyperuricemia,HUA)的作用机制,利用中药系统药理数据库和分析平台(Traditional Chinese medicine systems pharmacology database and analysis platform,TCMSP)预测核桃活性成分的作用靶点,运用OMIM、GeneCards和DrugBank数据库检索HUA疾病靶点。使用Cytoscape 3.7.2软件进行作用靶点-活性成分-疾病网络绘制,利用Metascape平台进行富集分析。使用AutoDockTools 1.5.6软件进行分子对接,对活性成分及重要靶点的结合度进行验证。结果表明,共筛选出核桃活性成分5种,与HUA有交集靶点16个;富集程度较高的信号通路包括癌症信号通路、化学致癌-受体激活信号通路、糖尿病并发症中的晚期糖基化产物-晚期糖基化终末产物受体信号通路和卡波西肉瘤相关疱疹病毒信号通路;通过分子对接验证试验可知,核桃中主要活性成分与重要靶点结合紧密。
