Transferability of five nuclear microsatellite markers (Jc-16, Jc-31, Jc-32, Jc-35 and Jc-37) that were originally developed for J. communis was tested to J. procera. Jc-31 & Jc-37 showed successful amplifications...Transferability of five nuclear microsatellite markers (Jc-16, Jc-31, Jc-32, Jc-35 and Jc-37) that were originally developed for J. communis was tested to J. procera. Jc-31 & Jc-37 showed successful amplifications and polymorphism in J. procera. Jc-35 which had been reported as polymorphic in J. communis was monomorphic in J. procera while the primer pair for Jc-32 failed to record any amplification. The remaining one primer pair (Jc-16) showed double loci ampli-fication in both J. procera and the control J. communis suggesting further examination of the primer pair and its binding sites. Genetic variation of six Ethiopian J. procera populations: Chilimo, Goba, Menagesha-Suba, Wef-Washa, Yabelo and Ziquala was assessed based on the two polymorphic loci (Jc-31 & Jc-37) in 20 - 24 individuals of each population. From these two loci, a total of 41 alleles could be retrieved. Two populations that are located south east of the Great Rift Valley together harboured 75% of private alleles signifying their deviant geo-ecological zones and suggesting special consideration for conservation. Chilimo, which is at the western margin of Juniper habitat in Ethiopian central highlands scored the highest fixation (FIS = 0.584) entailing lower immigrant genes and hence higher inbreeding. The AMOVA revealed that 97% of the variation resided within the?population while still among population variation was significant展开更多
The advantage of using plants in nanoparticles synthesis is that they are easily available, safe to handle and possess a broad variability of metabolites such as antioxidants, nucleotides and vitamins. The aim of this...The advantage of using plants in nanoparticles synthesis is that they are easily available, safe to handle and possess a broad variability of metabolites such as antioxidants, nucleotides and vitamins. The aim of this study was to investigate the effects of Green and Zimbro tea and also Green coconut water as a reducing and stabilizer agent in gold nanoparticle synthesis. The gold nanoparticles were characterized by UV-Vis absorption spectroscopy, X-ray diffraction (XRD), Dynamic light scattering (DLS) and Transmission electron microscopy (TEM) analysis. Their physical stability was determined using a UV-Vis spectrophotometer over several days during storage at room temperature. We observed that green chemical process to obtain gold nanoparticles did not require any external chemicals reagent for stabilization of nanoparticulate. Absorption measurements indicated that the plasmon resonance wavelength appears around 530 nm. X-ray diffracto-grams of gold nanoparticles evidenced the presence of Au-rich (fcc) phases. TEM analysis showed a homogeneous dispersion of nanoparticles and some agglomerates. Differences in size and shape of the nanoparticles were observed. Zeta potential of AuNPs synthetized in presence of Green tea was -33 mV indicating stability of the synthesized nanoparticles.展开更多
基金The German Academic Exchange Service(DAAD)is appreciated for financial support to the first author
文摘Transferability of five nuclear microsatellite markers (Jc-16, Jc-31, Jc-32, Jc-35 and Jc-37) that were originally developed for J. communis was tested to J. procera. Jc-31 & Jc-37 showed successful amplifications and polymorphism in J. procera. Jc-35 which had been reported as polymorphic in J. communis was monomorphic in J. procera while the primer pair for Jc-32 failed to record any amplification. The remaining one primer pair (Jc-16) showed double loci ampli-fication in both J. procera and the control J. communis suggesting further examination of the primer pair and its binding sites. Genetic variation of six Ethiopian J. procera populations: Chilimo, Goba, Menagesha-Suba, Wef-Washa, Yabelo and Ziquala was assessed based on the two polymorphic loci (Jc-31 & Jc-37) in 20 - 24 individuals of each population. From these two loci, a total of 41 alleles could be retrieved. Two populations that are located south east of the Great Rift Valley together harboured 75% of private alleles signifying their deviant geo-ecological zones and suggesting special consideration for conservation. Chilimo, which is at the western margin of Juniper habitat in Ethiopian central highlands scored the highest fixation (FIS = 0.584) entailing lower immigrant genes and hence higher inbreeding. The AMOVA revealed that 97% of the variation resided within the?population while still among population variation was significant
文摘The advantage of using plants in nanoparticles synthesis is that they are easily available, safe to handle and possess a broad variability of metabolites such as antioxidants, nucleotides and vitamins. The aim of this study was to investigate the effects of Green and Zimbro tea and also Green coconut water as a reducing and stabilizer agent in gold nanoparticle synthesis. The gold nanoparticles were characterized by UV-Vis absorption spectroscopy, X-ray diffraction (XRD), Dynamic light scattering (DLS) and Transmission electron microscopy (TEM) analysis. Their physical stability was determined using a UV-Vis spectrophotometer over several days during storage at room temperature. We observed that green chemical process to obtain gold nanoparticles did not require any external chemicals reagent for stabilization of nanoparticulate. Absorption measurements indicated that the plasmon resonance wavelength appears around 530 nm. X-ray diffracto-grams of gold nanoparticles evidenced the presence of Au-rich (fcc) phases. TEM analysis showed a homogeneous dispersion of nanoparticles and some agglomerates. Differences in size and shape of the nanoparticles were observed. Zeta potential of AuNPs synthetized in presence of Green tea was -33 mV indicating stability of the synthesized nanoparticles.
