To understand the relationship between expression of P53 protein and HPV16/18 infection in laryngeal papillomas, PCR and immunohistochemical tech-niques were used to examine the paraffin-embedded tissue samples of lar...To understand the relationship between expression of P53 protein and HPV16/18 infection in laryngeal papillomas, PCR and immunohistochemical tech-niques were used to examine the paraffin-embedded tissue samples of laryngeal pa-pillomas from 35 subjects. HPV 16/18-DNA was found in 24 cases of laryngeal papillomas (68. 8 %). Overexpression of P53 protein was detected in 19 cases (54. 3 % ). Both HPV16/18-DNA and overexpression of P53 protein were demon-strated in 12 cases of laryngeal papillomas (34. 3 % ). Our results suggest that HPV16/l8 infection and P53 gene mutation are associated with pathogenesis of la-ryngeal papillomas. The relation between HPV infection and P53 mutation in tis-sues of laryngeal papillomas remains to be clarified.展开更多
Objective：Laryngeal squamous cell carcinoma（LSCC） is a common malignant tumor in Northeast China and is frequently associated with well-established risk factors like smoking and alcohol abuse.Human papilloma virus...Objective：Laryngeal squamous cell carcinoma（LSCC） is a common malignant tumor in Northeast China and is frequently associated with well-established risk factors like smoking and alcohol abuse.Human papilloma virus（HPV） is an epitheliotropic oncogenic virus that has been detected in a variety of head and neck tumors including LSCC.This retrospective study was to investigate the prevalence of HPV infection in patients with LSCC.Methods：In situ hybridization was performed in 99 patients with LSCC to detect the expression of HPV-16 E6 mRNA.Results：The positive rate of HPV16 E6 mRNA was 36.36%（36/99） in laryngeal squamous cell carcinoma（LSCC）,whereas only 3 of 50（6%） specimens of the normal laryngeal mucosa as a control group showed positive results（P0.05）.Additionally,there was no corelation between HPV16 and age,gender,clinical stage,nodal status and tumor site（P0.05）.Conclusion：The results suggest that the increased prevalence of HPV infection compared to normal laryngeal mucosa and the fact that high-risk HPV types（especially type 16） were the most frequently identified do not allow the exclusion of HPV as a risk factor in laryngeal squamous cell carcinoma.However,their clinical value remains to be further investigated.展开更多
目的探讨窄带成像技术(narrow band imaging,NBI)应用于喉乳头状瘤诊断的可行性。方法以2015年6月至2017年6月就诊的疑似喉乳头状瘤患者72例为研究对象,所有患者均行电子鼻咽喉镜普通白光模式和NBI模式检查,比较两种模式对病灶的检出率...目的探讨窄带成像技术(narrow band imaging,NBI)应用于喉乳头状瘤诊断的可行性。方法以2015年6月至2017年6月就诊的疑似喉乳头状瘤患者72例为研究对象,所有患者均行电子鼻咽喉镜普通白光模式和NBI模式检查,比较两种模式对病灶的检出率,以病理结果为诊断金标准,比较电子鼻咽喉镜NBI模式和普通白光模式对喉乳头状瘤诊断的准确性、敏感性和特异性。结果 72例患者共检出98处病灶,其中,电子鼻咽喉镜NBI模式发现98处病灶,而白光模式仅发现70处病灶,检出率为71.43%(70/98)。98处病灶病理确诊为乳头状瘤82处,非乳头状瘤16处;与病理诊断比较,NBI模式对喉乳头状瘤诊断的准确性、敏感性和特异性分别为90.82%(89/98)、95.12%(78/82)和68.75%(11/16);普通白光模式分别为74.49%(73/98)、79.27%(65/82)和50.00%(8/16),NBI模式诊断喉乳头状瘤的准确性和敏感性明显高于普通白光模式,且差异有统计学意义(均为P<0.05),两种模式电子喉镜诊断喉乳头状瘤的特异性无统计学差异(P>0.05)。结论电子鼻咽喉镜NBI模式可提高喉乳头状瘤的检出率及诊断喉乳头状瘤的准确性和敏感性。展开更多
文摘To understand the relationship between expression of P53 protein and HPV16/18 infection in laryngeal papillomas, PCR and immunohistochemical tech-niques were used to examine the paraffin-embedded tissue samples of laryngeal pa-pillomas from 35 subjects. HPV 16/18-DNA was found in 24 cases of laryngeal papillomas (68. 8 %). Overexpression of P53 protein was detected in 19 cases (54. 3 % ). Both HPV16/18-DNA and overexpression of P53 protein were demon-strated in 12 cases of laryngeal papillomas (34. 3 % ). Our results suggest that HPV16/l8 infection and P53 gene mutation are associated with pathogenesis of la-ryngeal papillomas. The relation between HPV infection and P53 mutation in tis-sues of laryngeal papillomas remains to be clarified.
文摘Objective：Laryngeal squamous cell carcinoma（LSCC） is a common malignant tumor in Northeast China and is frequently associated with well-established risk factors like smoking and alcohol abuse.Human papilloma virus（HPV） is an epitheliotropic oncogenic virus that has been detected in a variety of head and neck tumors including LSCC.This retrospective study was to investigate the prevalence of HPV infection in patients with LSCC.Methods：In situ hybridization was performed in 99 patients with LSCC to detect the expression of HPV-16 E6 mRNA.Results：The positive rate of HPV16 E6 mRNA was 36.36%（36/99） in laryngeal squamous cell carcinoma（LSCC）,whereas only 3 of 50（6%） specimens of the normal laryngeal mucosa as a control group showed positive results（P0.05）.Additionally,there was no corelation between HPV16 and age,gender,clinical stage,nodal status and tumor site（P0.05）.Conclusion：The results suggest that the increased prevalence of HPV infection compared to normal laryngeal mucosa and the fact that high-risk HPV types（especially type 16） were the most frequently identified do not allow the exclusion of HPV as a risk factor in laryngeal squamous cell carcinoma.However,their clinical value remains to be further investigated.
文摘目的探讨窄带成像技术(narrow band imaging,NBI)应用于喉乳头状瘤诊断的可行性。方法以2015年6月至2017年6月就诊的疑似喉乳头状瘤患者72例为研究对象,所有患者均行电子鼻咽喉镜普通白光模式和NBI模式检查,比较两种模式对病灶的检出率,以病理结果为诊断金标准,比较电子鼻咽喉镜NBI模式和普通白光模式对喉乳头状瘤诊断的准确性、敏感性和特异性。结果 72例患者共检出98处病灶,其中,电子鼻咽喉镜NBI模式发现98处病灶,而白光模式仅发现70处病灶,检出率为71.43%(70/98)。98处病灶病理确诊为乳头状瘤82处,非乳头状瘤16处;与病理诊断比较,NBI模式对喉乳头状瘤诊断的准确性、敏感性和特异性分别为90.82%(89/98)、95.12%(78/82)和68.75%(11/16);普通白光模式分别为74.49%(73/98)、79.27%(65/82)和50.00%(8/16),NBI模式诊断喉乳头状瘤的准确性和敏感性明显高于普通白光模式,且差异有统计学意义(均为P<0.05),两种模式电子喉镜诊断喉乳头状瘤的特异性无统计学差异(P>0.05)。结论电子鼻咽喉镜NBI模式可提高喉乳头状瘤的检出率及诊断喉乳头状瘤的准确性和敏感性。
