K+ channel blockers of scorpion venoms are of important value in studyingpharmacology and physiology of specific K+ channel of celis. Based on the amino acid sequences of BmP01 previously characterized as a small-cond...K+ channel blockers of scorpion venoms are of important value in studyingpharmacology and physiology of specific K+ channel of celis. Based on the amino acid sequences of BmP01 previously characterized as a small-conductance Ca2+-activated K+ channel blocker, two 'back to back' degenarate primers have been designed and synthesized for inverse PCR strategy, its full-length cDNA has been cloned from the venom gland of the Chinese scorpion Buthus martensii. The cDNA is composed of 3 parts: 5’ UTR, oRF and 3’ UTR. The flanking sequence of translation initiation codon ATG is AAAKTGA, which is highly conserved in scorpion Na+ channel toxin and protozoan genes, suggesting that these genes may have followed a common mechanism for translation initiation. The 3’ UTR contains poly(A) signal AATAAA. The open reading frame encodes a precursor of 57 residues with a signal peptide of 28 residues and a mature peptide of 29 residues. The signal peptide is rich in hydrophobic amino acid residues and its length is展开更多
文摘K+ channel blockers of scorpion venoms are of important value in studyingpharmacology and physiology of specific K+ channel of celis. Based on the amino acid sequences of BmP01 previously characterized as a small-conductance Ca2+-activated K+ channel blocker, two 'back to back' degenarate primers have been designed and synthesized for inverse PCR strategy, its full-length cDNA has been cloned from the venom gland of the Chinese scorpion Buthus martensii. The cDNA is composed of 3 parts: 5’ UTR, oRF and 3’ UTR. The flanking sequence of translation initiation codon ATG is AAAKTGA, which is highly conserved in scorpion Na+ channel toxin and protozoan genes, suggesting that these genes may have followed a common mechanism for translation initiation. The 3’ UTR contains poly(A) signal AATAAA. The open reading frame encodes a precursor of 57 residues with a signal peptide of 28 residues and a mature peptide of 29 residues. The signal peptide is rich in hydrophobic amino acid residues and its length is
基金This work was supported by the National Natural Science Foundation of China (No. 30170302 39470238+3 种基金 39070323 39860249)the Climbing Project of Ministry of Science and Technology of China (No. P8505) the National Basic Research Priorities Programmeof China (No. G19999054000) and the Basic Research Program of Shanghai Municipal Commission for Science and Technology (No.O2JC14011).
