Objective This study investigated the impact of occupational mercury(Hg) exposure on human gene transcription and expression, and its potential biological mechanisms.Methods Differentially expressed genes related to H...Objective This study investigated the impact of occupational mercury(Hg) exposure on human gene transcription and expression, and its potential biological mechanisms.Methods Differentially expressed genes related to Hg exposure were identified and validated using gene expression microarray analysis and extended validation. Hg-exposed cell models and PTEN lowexpression models were established in vitro using 293T cells. PTEN gene expression was assessed using qRT-PCR, and Western blotting was used to measure PTEN, AKT, and PI3K protein levels. IL-6 expression was determined by ELISA.Results Combined findings from gene expression microarray analysis, bioinformatics, and population expansion validation indicated significant downregulation of the PTEN gene in the high-concentration Hg exposure group. In the Hg-exposed cell model(25 and 10 μmol/L), a significant decrease in PTEN expression was observed, accompanied by a significant increase in PI3K, AKT, and IL-6 expression.Similarly, a low-expression cell model demonstrated that PTEN gene knockdown led to a significant decrease in PTEN protein expression and a substantial increase in PI3K, AKT, and IL-6 levels.Conclusion This is the first study to report that Hg exposure downregulates the PTEN gene, activates the PI3K/AKT regulatory pathway, and increases the expression of inflammatory factors, ultimately resulting in kidney inflammation.展开更多
One caveat to the dinosaur’s extinction is the conclusion that avian dinosaurs survived and became ancestors of birds. Their mobility enabled them to migrate great distances and find the nutrients needed to survive. ...One caveat to the dinosaur’s extinction is the conclusion that avian dinosaurs survived and became ancestors of birds. Their mobility enabled them to migrate great distances and find the nutrients needed to survive. Given this scenario, could the current observable migration of birds (the “dinosaurian offspring”) now be related? Migration is the regular seasonal movement undertaken by many species of birds, with the most common pattern, flying north in the Northern spring to breed in the temperate or Arctic summer and returning in the Northern autumn to wintering grounds in warmer regions of the south. The primary motivation for migration appears to be food. None of the major North-South migratory pathways fly over the Caribbean but three main fly ways, past to the west of the theorized K-T impact centre. Due to their ability to fly, the “avian Dinosaurs” adapted and survived very quickly in response to the disaster that marked the K-T boundary. It is an interesting speculation that the avian migration that we witness today is rooted in an event that occurred 66 million years ago! But it does explain why the migratory birds mostly fly from Polar summer to polar summer when they could just be as easily fly from Polar zone to the warmer equatorial region and back. In the recent article in Nature by Melanie During about identifying the late spring timing of the “Astro disaster”, it can be cited as consistent with my speculation. A late April early May Impact as suggested by During would have seen these migrations completely. The western migratory routes would have been found to be “luxurious” in vegetation in that first northern autumn after the “Astro-impact” while all eastern routes would have still been barren.展开更多
k holomorphic functions are a type of generation of holomorphic functions.In this paper,a nonlinear boundary value problem for k holomorphic functions is primarily discussed on generalized polycylinders in C^(2).The e...k holomorphic functions are a type of generation of holomorphic functions.In this paper,a nonlinear boundary value problem for k holomorphic functions is primarily discussed on generalized polycylinders in C^(2).The existence of the solution for the problem is studied in detail with the help of the boundary properties of Cauchy type singular integral operators with a k holomorphic kernel.Furthermore,the integral representation for the solution is obtained.展开更多
The existence of the Cretaceous-Tertiary （K/T） boundary in the non-marine succession is expected at Jiayin in the Heilongjiang River area, China. Zircons from a tuff sample from the Baishantou Member of Wuyun Format...The existence of the Cretaceous-Tertiary （K/T） boundary in the non-marine succession is expected at Jiayin in the Heilongjiang River area, China. Zircons from a tuff sample from the Baishantou Member of Wuyun Formation in Jiayin were analyzed by the laser ablation inductively coupled plasma mass spectrometry （LA-ICP-MS） U-Pb dating and fission-track dating methods. Ages of 64.1±0.7 Ma （U-Pb） and 61.7±1.8 Ma （fission-track dating） were obtained, which allow reevaluation of a previously reported late Maastrichian age for the tuff layer that was in conflict with the paleontological evidence. These results confirm the Danian age of the section in agreement with the paleontological evidence.展开更多
During the last decades,several integrated studies of Tethyan Jurassic/Cretaceous boundary sections from different countries were published with the objective to indicate problems for the selection of biological,chemi...During the last decades,several integrated studies of Tethyan Jurassic/Cretaceous boundary sections from different countries were published with the objective to indicate problems for the selection of biological,chemical or physical markers suitable for identification of the Jurassic/Cretaceous boundary-the only system boundary within the Phanerozoic still not fixed by GSSP.Drawing the boundary between the Jurassic and Cretaceous systems is a matter of global scale discussions.The problem of proposing possible J/K boundary stratotypes results from lack of a global index fossils, global sea level drop,paleogeographic changes causing development of isolated facies areas,as well as from the effect of Late Cimmerian Orogeny.This contribution summarizes and comments data on J/K boundary interval obtained from several important Tethyan sections and shows still existing problems and discrepancies in its determination.展开更多
In this paper, the problem of a crack perpendicular to and terminating at an interface in bimaterial structure with finite boundaries is investigated. The dislocation simulation method and boundary collocation approac...In this paper, the problem of a crack perpendicular to and terminating at an interface in bimaterial structure with finite boundaries is investigated. The dislocation simulation method and boundary collocation approach are used to derive and solve the basic equations. Two kinds of loading form are considered when the crack lies in a softer or a stiffer material, one is an ideal loading and the other one fits to the practical experiment loading. Complete solutions of the stress field including the T stress are obtained as well as the stress intensity factors. Influences of T stress on the stress field ahead of the crack tip are studied. Finite boundary effects on the stress intensity factors are emphasized. Comparisons with the problem presented by Chen et al. (Int. J. Solids and Structure, 2003, 40, 2731–2755) are discussed also.展开更多
基金supported by the Jiangsu Province’s Outstanding Medical Academic Leader Program [CXTDA2017029]the Jiangsu Provincial Key Medical Discipline [ZDXK202249].
文摘Objective This study investigated the impact of occupational mercury(Hg) exposure on human gene transcription and expression, and its potential biological mechanisms.Methods Differentially expressed genes related to Hg exposure were identified and validated using gene expression microarray analysis and extended validation. Hg-exposed cell models and PTEN lowexpression models were established in vitro using 293T cells. PTEN gene expression was assessed using qRT-PCR, and Western blotting was used to measure PTEN, AKT, and PI3K protein levels. IL-6 expression was determined by ELISA.Results Combined findings from gene expression microarray analysis, bioinformatics, and population expansion validation indicated significant downregulation of the PTEN gene in the high-concentration Hg exposure group. In the Hg-exposed cell model(25 and 10 μmol/L), a significant decrease in PTEN expression was observed, accompanied by a significant increase in PI3K, AKT, and IL-6 expression.Similarly, a low-expression cell model demonstrated that PTEN gene knockdown led to a significant decrease in PTEN protein expression and a substantial increase in PI3K, AKT, and IL-6 levels.Conclusion This is the first study to report that Hg exposure downregulates the PTEN gene, activates the PI3K/AKT regulatory pathway, and increases the expression of inflammatory factors, ultimately resulting in kidney inflammation.
文摘One caveat to the dinosaur’s extinction is the conclusion that avian dinosaurs survived and became ancestors of birds. Their mobility enabled them to migrate great distances and find the nutrients needed to survive. Given this scenario, could the current observable migration of birds (the “dinosaurian offspring”) now be related? Migration is the regular seasonal movement undertaken by many species of birds, with the most common pattern, flying north in the Northern spring to breed in the temperate or Arctic summer and returning in the Northern autumn to wintering grounds in warmer regions of the south. The primary motivation for migration appears to be food. None of the major North-South migratory pathways fly over the Caribbean but three main fly ways, past to the west of the theorized K-T impact centre. Due to their ability to fly, the “avian Dinosaurs” adapted and survived very quickly in response to the disaster that marked the K-T boundary. It is an interesting speculation that the avian migration that we witness today is rooted in an event that occurred 66 million years ago! But it does explain why the migratory birds mostly fly from Polar summer to polar summer when they could just be as easily fly from Polar zone to the warmer equatorial region and back. In the recent article in Nature by Melanie During about identifying the late spring timing of the “Astro disaster”, it can be cited as consistent with my speculation. A late April early May Impact as suggested by During would have seen these migrations completely. The western migratory routes would have been found to be “luxurious” in vegetation in that first northern autumn after the “Astro-impact” while all eastern routes would have still been barren.
基金the NSF of China(11571089,11871191)the NSF of Henan Province(222300420397)+1 种基金the NSF of Hebei Province(A2022208007)the Key Foundation of Hebei Normal University(L2018Z01)。
文摘k holomorphic functions are a type of generation of holomorphic functions.In this paper,a nonlinear boundary value problem for k holomorphic functions is primarily discussed on generalized polycylinders in C^(2).The existence of the solution for the problem is studied in detail with the help of the boundary properties of Cauchy type singular integral operators with a k holomorphic kernel.Furthermore,the integral representation for the solution is obtained.
基金the Project NSFC No30220130698the Project "111" of China for their support the National Science Council,Taiwan(grant 97-2811-M- 002-040)
文摘The existence of the Cretaceous-Tertiary （K/T） boundary in the non-marine succession is expected at Jiayin in the Heilongjiang River area, China. Zircons from a tuff sample from the Baishantou Member of Wuyun Formation in Jiayin were analyzed by the laser ablation inductively coupled plasma mass spectrometry （LA-ICP-MS） U-Pb dating and fission-track dating methods. Ages of 64.1±0.7 Ma （U-Pb） and 61.7±1.8 Ma （fission-track dating） were obtained, which allow reevaluation of a previously reported late Maastrichian age for the tuff layer that was in conflict with the paleontological evidence. These results confirm the Danian age of the section in agreement with the paleontological evidence.
基金the APVV-0280-07,APVV-0248-07APVV- 0465-06+1 种基金APVT 51-011305VEGA 0196 and 0065
文摘During the last decades,several integrated studies of Tethyan Jurassic/Cretaceous boundary sections from different countries were published with the objective to indicate problems for the selection of biological,chemical or physical markers suitable for identification of the Jurassic/Cretaceous boundary-the only system boundary within the Phanerozoic still not fixed by GSSP.Drawing the boundary between the Jurassic and Cretaceous systems is a matter of global scale discussions.The problem of proposing possible J/K boundary stratotypes results from lack of a global index fossils, global sea level drop,paleogeographic changes causing development of isolated facies areas,as well as from the effect of Late Cimmerian Orogeny.This contribution summarizes and comments data on J/K boundary interval obtained from several important Tethyan sections and shows still existing problems and discrepancies in its determination.
基金The project supported by the National Natural Science Foundation of China(10202023 and 10272103)the Key Project of CAS(KJCX2-SW-L2).
文摘In this paper, the problem of a crack perpendicular to and terminating at an interface in bimaterial structure with finite boundaries is investigated. The dislocation simulation method and boundary collocation approach are used to derive and solve the basic equations. Two kinds of loading form are considered when the crack lies in a softer or a stiffer material, one is an ideal loading and the other one fits to the practical experiment loading. Complete solutions of the stress field including the T stress are obtained as well as the stress intensity factors. Influences of T stress on the stress field ahead of the crack tip are studied. Finite boundary effects on the stress intensity factors are emphasized. Comparisons with the problem presented by Chen et al. (Int. J. Solids and Structure, 2003, 40, 2731–2755) are discussed also.
