It is first demonstrated that dipyridamole (DP) and radiation were capable of significantly inhibiting, independently and synerglcally, clonogenlc growth in the two kinds of K562 cell lines, adriamycin (ADM) -sensitiv...It is first demonstrated that dipyridamole (DP) and radiation were capable of significantly inhibiting, independently and synerglcally, clonogenlc growth in the two kinds of K562 cell lines, adriamycin (ADM) -sensitive and ADM- resistant. DP or radiation alone Increased clonogenlc Inhibition rate (CIR) in the two kinds of cell lines in a dose- dependent fashion. DP potentiated radiosensitivity and radiation increased inhibition of DP in the two kinds of cell lines. K562/ ADM cell lines were higher sensitive to DP. radiation and combination of them than K562 cell lines (P<0. 01). There was stronger synergic inhibition of clonogenlc growth in the two kinds of cell lines when pretreated with DP than when posttreated with DP (P<0. 01).展开更多
It was first reported here that verupamil(VP) and electric beam radiation(EBR) were capable of inhibiting,independently or synergically,clonogenic growth in two kinds of K562 cell lines, adriamycin(ADM)-sensitive and ...It was first reported here that verupamil(VP) and electric beam radiation(EBR) were capable of inhibiting,independently or synergically,clonogenic growth in two kinds of K562 cell lines, adriamycin(ADM)-sensitive and ADM-resistant(K562/S and K562/ADM).Results showed that clonogenic rate(CGR) decreased by 3%-99.9% in the prasence of dependent dose-ADM(3.8μg/ml) in K562/ADM cell lines,while treated with 0.5μM-6μM of VP.VP was capable of potentiating radiosensitivity in K562/S and K562/ADM cell lines,whether before or after exposure of them to electric beam radiation,and significantly reduced CGR in these kinds of cell lines(P<0.01).展开更多
OBJECTIVE To investigate the effects of survivin antisense oligodeoxy-nucleotid (ASODN) on proliferation and apoptosis in the chronic myeloid leukemia cell line K562. METHODS Different concentrations of an antisense o...OBJECTIVE To investigate the effects of survivin antisense oligodeoxy-nucleotid (ASODN) on proliferation and apoptosis in the chronic myeloid leukemia cell line K562. METHODS Different concentrations of an antisense oligodeoxy-nucleotid and control sequence (scrambled ODN) targeting the survivin gene were transferred into K562 by a lipofectin reagent. The MTT assay was used to measure the growth inhibitory rate, IC50, and to observe the cytotoxicity of survivin ASODN in the K562 cells. The morphologic changes in the nucleus and the apoptotic rate were observed by Hoechst33342/PI staining. Caspase-3 activity was evaluated by a kinase activity assay. The changes of survivin protein expression after transfection were detected by Western blots. RESULTS Eight hours after transfection, fluorescence in the K562 cells was well distributed. Treatment of the cells for 44 h with different concentrations of survivin ASODN produced a IC50 of 800 nmol/L. The growth inhibitory rate with 200, 400, 600 and 1000 nmol/L of survivin ASODN was 15.8±1.6%, 23.8±5.9%, 37.1±5.6% and 77.3±2.5% respectively. After 36 h of of survivin ASODN treatment, distinct morphologic changes characteristic of cell apoptosis such as karyopyknosis and conglomeration were observed by Hoechst33342/PI staining. Caspase-3 activity increased significantly after treatment of the cells with different concentrations of survivin ASODN(P<0.01)and following treatment with 800 nmol/L survivin ASODN, survivin expression decreased significantly. CONCLUSION Survivin ASODN exerts an anti-cancer effect by inducing apoptosis in K562 leukaemia cells. Up-regulated expression of caspase-3 may play a role in this process.展开更多
It is well accepted in China that elder ginsengs have more bioactivity and value than younger ones. However, there is little research about the comparison of beneficial effects of ginsengs with different ages. In this...It is well accepted in China that elder ginsengs have more bioactivity and value than younger ones. However, there is little research about the comparison of beneficial effects of ginsengs with different ages. In this study, ginseng root extracts (GRE) were extracted from ginsengs of 5, 8, 12, 14, and 16 years old, respectively, using 55% ethanol and their effects on human leukemic K562 cells within 48 hours were tested by using Cell Counting Kit-8. The results show that there are significant increases in the cell viability of all the GRE groups compared with Control group within 32 hours. Furthermore, the growth curves of GRE groups were obviously distinct from each other. The cell viability of 5-year-old and 8-year-old GRE groups kept a rapid increase while that of 16-year-old GRE group showed a strong fluctuation within 28 hours. Our results demonstrate that root extracts from ginsengs of different ages contain different bioactivity constituents and have different effects on cell.展开更多
本研究观察程序性细胞死亡5基因(programmed cell death 5,PDCD5)重组腺病毒转染K562细胞后对化疗药物依托泊甙的增敏作用。利用AdMaxTM腺病毒载体包装系统,通过同源重组方法构建Ad-PDCD5重组腺病毒及对照腺病毒Ad-null及Ad-eGFP;用不...本研究观察程序性细胞死亡5基因(programmed cell death 5,PDCD5)重组腺病毒转染K562细胞后对化疗药物依托泊甙的增敏作用。利用AdMaxTM腺病毒载体包装系统,通过同源重组方法构建Ad-PDCD5重组腺病毒及对照腺病毒Ad-null及Ad-eGFP;用不同感染复数将Ad-eGFP、Ad-null或Ad-PDCD5转染人白血病细胞系,实时定量PCR检测PDCD5mRNA的相对表达水平;利用MTT法及Annexin-V-FITC/PI双染色流式细胞术观察依托泊甙对转染后K562细胞增殖与凋亡的影响。结果表明:Ad-eGFP腺病毒对白血病细胞系K562、Jurkat及CEM的转染效率可达60%-86%。Ad-PDCD5重组腺病毒能梯度增加K562细胞PDCD5 mRNA的相对表达水平,腺病毒介导的PDCD5基因转移促进依托泊甙诱导的K562细胞凋亡。结论:PDCD5重组腺病毒可能成为化疗药物的增敏剂。展开更多
文摘It is first demonstrated that dipyridamole (DP) and radiation were capable of significantly inhibiting, independently and synerglcally, clonogenlc growth in the two kinds of K562 cell lines, adriamycin (ADM) -sensitive and ADM- resistant. DP or radiation alone Increased clonogenlc Inhibition rate (CIR) in the two kinds of cell lines in a dose- dependent fashion. DP potentiated radiosensitivity and radiation increased inhibition of DP in the two kinds of cell lines. K562/ ADM cell lines were higher sensitive to DP. radiation and combination of them than K562 cell lines (P<0. 01). There was stronger synergic inhibition of clonogenlc growth in the two kinds of cell lines when pretreated with DP than when posttreated with DP (P<0. 01).
文摘It was first reported here that verupamil(VP) and electric beam radiation(EBR) were capable of inhibiting,independently or synergically,clonogenic growth in two kinds of K562 cell lines, adriamycin(ADM)-sensitive and ADM-resistant(K562/S and K562/ADM).Results showed that clonogenic rate(CGR) decreased by 3%-99.9% in the prasence of dependent dose-ADM(3.8μg/ml) in K562/ADM cell lines,while treated with 0.5μM-6μM of VP.VP was capable of potentiating radiosensitivity in K562/S and K562/ADM cell lines,whether before or after exposure of them to electric beam radiation,and significantly reduced CGR in these kinds of cell lines(P<0.01).
文摘OBJECTIVE To investigate the effects of survivin antisense oligodeoxy-nucleotid (ASODN) on proliferation and apoptosis in the chronic myeloid leukemia cell line K562. METHODS Different concentrations of an antisense oligodeoxy-nucleotid and control sequence (scrambled ODN) targeting the survivin gene were transferred into K562 by a lipofectin reagent. The MTT assay was used to measure the growth inhibitory rate, IC50, and to observe the cytotoxicity of survivin ASODN in the K562 cells. The morphologic changes in the nucleus and the apoptotic rate were observed by Hoechst33342/PI staining. Caspase-3 activity was evaluated by a kinase activity assay. The changes of survivin protein expression after transfection were detected by Western blots. RESULTS Eight hours after transfection, fluorescence in the K562 cells was well distributed. Treatment of the cells for 44 h with different concentrations of survivin ASODN produced a IC50 of 800 nmol/L. The growth inhibitory rate with 200, 400, 600 and 1000 nmol/L of survivin ASODN was 15.8±1.6%, 23.8±5.9%, 37.1±5.6% and 77.3±2.5% respectively. After 36 h of of survivin ASODN treatment, distinct morphologic changes characteristic of cell apoptosis such as karyopyknosis and conglomeration were observed by Hoechst33342/PI staining. Caspase-3 activity increased significantly after treatment of the cells with different concentrations of survivin ASODN(P<0.01)and following treatment with 800 nmol/L survivin ASODN, survivin expression decreased significantly. CONCLUSION Survivin ASODN exerts an anti-cancer effect by inducing apoptosis in K562 leukaemia cells. Up-regulated expression of caspase-3 may play a role in this process.
文摘It is well accepted in China that elder ginsengs have more bioactivity and value than younger ones. However, there is little research about the comparison of beneficial effects of ginsengs with different ages. In this study, ginseng root extracts (GRE) were extracted from ginsengs of 5, 8, 12, 14, and 16 years old, respectively, using 55% ethanol and their effects on human leukemic K562 cells within 48 hours were tested by using Cell Counting Kit-8. The results show that there are significant increases in the cell viability of all the GRE groups compared with Control group within 32 hours. Furthermore, the growth curves of GRE groups were obviously distinct from each other. The cell viability of 5-year-old and 8-year-old GRE groups kept a rapid increase while that of 16-year-old GRE group showed a strong fluctuation within 28 hours. Our results demonstrate that root extracts from ginsengs of different ages contain different bioactivity constituents and have different effects on cell.
文摘本研究观察程序性细胞死亡5基因(programmed cell death 5,PDCD5)重组腺病毒转染K562细胞后对化疗药物依托泊甙的增敏作用。利用AdMaxTM腺病毒载体包装系统,通过同源重组方法构建Ad-PDCD5重组腺病毒及对照腺病毒Ad-null及Ad-eGFP;用不同感染复数将Ad-eGFP、Ad-null或Ad-PDCD5转染人白血病细胞系,实时定量PCR检测PDCD5mRNA的相对表达水平;利用MTT法及Annexin-V-FITC/PI双染色流式细胞术观察依托泊甙对转染后K562细胞增殖与凋亡的影响。结果表明:Ad-eGFP腺病毒对白血病细胞系K562、Jurkat及CEM的转染效率可达60%-86%。Ad-PDCD5重组腺病毒能梯度增加K562细胞PDCD5 mRNA的相对表达水平,腺病毒介导的PDCD5基因转移促进依托泊甙诱导的K562细胞凋亡。结论:PDCD5重组腺病毒可能成为化疗药物的增敏剂。
