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辣椒抗炭疽病主效基因的定位及标记开发 被引量:11
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作者 刘议蔚 张正海 +3 位作者 曹亚从 赵红 王立浩 张宝玺 《中国蔬菜》 北大核心 2016年第11期20-24,共5页
以高抗炭疽病的辣椒材料PBC932(Capsicum chinense)为父本,以感病辣椒材料77013(Capsicum annuum)为母本和回交亲本,获得包含220个和129个单株的BC_3S_1和BC_4S_1群体材料,通过对各群体进行尖孢炭疽菌(Colletotrichum acutatum)抗性鉴定... 以高抗炭疽病的辣椒材料PBC932(Capsicum chinense)为父本,以感病辣椒材料77013(Capsicum annuum)为母本和回交亲本,获得包含220个和129个单株的BC_3S_1和BC_4S_1群体材料,通过对各群体进行尖孢炭疽菌(Colletotrichum acutatum)抗性鉴定和SNP(KASPar)分子标记分析,发现UN27353_1781标记与辣椒绿熟期炭疽病抗性基因AnR_(GO)5紧密连锁,遗传距离为2cM。进一步利用标记UN27353_1781对BC_4S_2群体124个单株进行分子标记分型,选择准确率达92.9%,表明该标记可有效用于辣椒抗炭疽病分子标记辅助育种。 展开更多
关键词 辣椒 炭疽病 抗性 尖孢炭疽菌 kaspar标记
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DNA Marker Technologies in Plants and Applications for Crop Improvements 被引量:2
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作者 Djshwar Dhahir Lateef 《Journal of Biosciences and Medicines》 2015年第5期7-18,共12页
Over the past several decades, especially through traditional breeding programme, intensive attempts have been made for the improvement of a large number of cereal varieties which adjusted to diverse agro-ecologies. H... Over the past several decades, especially through traditional breeding programme, intensive attempts have been made for the improvement of a large number of cereal varieties which adjusted to diverse agro-ecologies. However, increasing biotic and abiotic stresses, increasing populations, and sharply reducing natural resources especially water for agricultural purposes, push the breeders for organizing and developing improved cereal varieties with higher yield potential. In combination with developments in agricultural technology, plant breeding has made remarkable progress in increasing crop yields for over a century. Molecular markers are widely employed in plant breeding. DNA markers are being used for the acceleration of plant selection through marker-assisted selection (MAS). Genes of agronomic and scientific importance can be isolated especially on the basis of their position on the genetic map by using molecular markers technologies. In this review, the current status of marker development technologies for crop improvements will be discussed. It will also provide an outlook into the future approaches and most widely used applications in plant breeding in crop plants on the basis of present development. 展开更多
关键词 RFLPS RAPDS SSRS AFLPS SNPS kaspar GBS
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