目的:初步研究78 k D的葡萄糖调节蛋白(the 78 k D glucose-regulated protein,GRP78)与乙型肝炎病毒(hepatitis B virus,HBV)的前S1蛋白(Pre S1)的相互作用位点。方法:利用PCR技术扩增GRP78的基因,将扩增的目的基因克隆至p W28载体质粒...目的:初步研究78 k D的葡萄糖调节蛋白(the 78 k D glucose-regulated protein,GRP78)与乙型肝炎病毒(hepatitis B virus,HBV)的前S1蛋白(Pre S1)的相互作用位点。方法:利用PCR技术扩增GRP78的基因,将扩增的目的基因克隆至p W28载体质粒,在大肠杆菌(Escherichia coli,E.coli)B834中表达,经过镍离子亲和层析柱纯化GRP78蛋白;将Pre S1 3个截短片段的重组质粒(p GST-Pre S1-X1/X2/X3)在B834中表达后,经过GST亲和层析柱纯化相应蛋白;利用蛋白质体外结合实验(pull down)、微量热泳动(microscale thermophoresis,MST)检测GRP78与Pre S1 3个截短片段的相互作用。结果:成功构建重组质粒p W28-GRP78;获得GRP78蛋白及Pre S1 3个截短片段的融合蛋白;pull down及MST实验验证了GRP78可以与Pre S1的3个片段结合,且GRP78与GST-Pre S1-X1结合效果最好。结论:利用分子克隆技术及蛋白质表达纯化技术,获得GRP78蛋白及Pre S1截短片段的融合蛋白,并初步筛选了Pre S1与GRP78的相互作用位点,为后续研究打基础。展开更多
The(2+1)-dimensional Konopelchenko–Dubrovsky equation is an important prototypic model in nonlinear physics, which can be applied to many fields. Various nonlinear excitations of the(2+1)-dimensional Konopelchenko–D...The(2+1)-dimensional Konopelchenko–Dubrovsky equation is an important prototypic model in nonlinear physics, which can be applied to many fields. Various nonlinear excitations of the(2+1)-dimensional Konopelchenko–Dubrovsky equation have been found by many methods. However, it is very difficult to find interaction solutions among different types of nonlinear excitations. In this paper, with the help of the Riccati equation, the(2+1)-dimensional Konopelchenko–Dubrovsky equation is solved by the consistent Riccati expansion(CRE). Furthermore, we obtain the soliton-cnoidal wave interaction solution of the(2+1)-dimensional Konopelchenko–Dubrovsky equation.展开更多
基金Supported by the National Natural Science Foundation of China under Grant Nos.11175092,11275123,11205092,and 10905038Shanghai Knowledge Service Platform for Trustworthy Internet of Things under Grant No.ZF1213+1 种基金Talent FundK.C.Wong Magna Fund in Ningbo University
文摘The(2+1)-dimensional Konopelchenko–Dubrovsky equation is an important prototypic model in nonlinear physics, which can be applied to many fields. Various nonlinear excitations of the(2+1)-dimensional Konopelchenko–Dubrovsky equation have been found by many methods. However, it is very difficult to find interaction solutions among different types of nonlinear excitations. In this paper, with the help of the Riccati equation, the(2+1)-dimensional Konopelchenko–Dubrovsky equation is solved by the consistent Riccati expansion(CRE). Furthermore, we obtain the soliton-cnoidal wave interaction solution of the(2+1)-dimensional Konopelchenko–Dubrovsky equation.
