Expression and bioinformatic analysis of lymphoma-associated novel gene KIAA0372

The purpose of this study was to explore the differentially expressed genes in lymph-node cells(LNC)of lymphomas and reactive lymph node hyperplasia,and to perform an initial bioinformatic analysis on a novel gene,KIAA0372,which is highly expressed in the LNC of lymphomas.mRNA extracted from LNC of lymphomas and reactive lymph node hyperplasia were respectively marked with biotin and hybridized with Gene Expression Chips,resulting in differentially expressed genes.Initial bioinformatic analysis was then performed on a novel gene named KIAA0372,whose function has not yet been explored.Its structure and genomic location,its product’s physical and chemical properties,subcellular localization and functional domains,were also predicted.Further,a systematic evolution analysis was performed on similar proteins from among several species.Using Gene Expression Chips,many differentially expressed genes were uncovered.Efficient bioinformatic analysis has fundamentally determined that KIAA0372 is an extracellular protein which may be involved in TGF-b signaling.Microarray is an efficient and high throughput strategy for detection of differentially expressed genes.And KIAA0372 is thought to be a potential target for tumor research using bioinformatic analysis.

新基因KIAA0372在淋巴瘤中的表达及其生物信息学分析

目的研究淋巴瘤和反应性增生淋巴结基因表达的差异,并采用生物信息学方法对一条淋巴瘤高表达基因 KIAA0372进行初步分析。方法分别将淋巴瘤淋巴结标本和反应性增生淋巴结标本的 mRNA 与表达谱芯片杂交,通过信号扫描、处理后获得两者的表达差异基因。并用生物信息学方法对一条无功能研究的新基因 KIAA0372进行初步分析,预测它的基因结构、染色体定位、编码蛋白质的理化性质、亚细胞定位、蛋白质功能域等信息。并对多物种中的相似性蛋白进行了系统进化分析。结果表达谱芯片共发现了152条差异表达基因。对淋巴瘤相关新基因 KIAA0372的上述性质进行了有效的预测,基本明确了该基因编码一胞外分泌蛋白,可能作为 TGFβ信号通路的一个配基参与淋巴瘤细胞的生成。结论表达谱芯片技术是一种研究肿瘤基因表达差异的有效的高通量研究方法。新基因 KIAA0372是一个有研究价值的新靶点。