The homodimeric kinesin-2 protein KIF17 functions in intracellular transport and spermiogenesis in mammals.However,its role in fish spermiogenesis has not been reported.Here,we aimed to clone full-length kif17 cDNA an...The homodimeric kinesin-2 protein KIF17 functions in intracellular transport and spermiogenesis in mammals.However,its role in fish spermiogenesis has not been reported.Here,we aimed to clone full-length kif17 cDNA and determine the molecular characteristics and expression patterns of KIF17 in Larimichthys polyactis spermiogenesis.The full-length cDNA of L.polyactis kif17(Lp-kif17)was sequenced and found to contain a 332-bp 5′untranslated region,480-bp 3′untranslated region,and 2433-bp open reading frame encoding 810 amino acids.Bioinformatics analyses showed that L.polyactis KIF17(Lp-KIF17)shared high sequence similarity with homologs in other animals and possessed an N-terminal motor domain with microtubule-binding sites and adenosine triphosphate(ATP)hydrolysis sites,a stalk domain containing two coiled-coil regions,and a C-terminal tail domain.The Lp-kif17 mRNA was widely expressed in various tissues,with the highest level in the brain,followed by that in the testis.Fluorescence in situ hybridization(FISH)analysis revealed that Lp-kif17 was continuously expressed in spermiogenesis,showing that it had potential functions in this process.Using immunofluorescence(IF)analysis,we found that Lp-KIF17 colocalized with tubulin and was transferred from the perinuclear cytoplasm to the side of spermatid where the tail forms during spermiogenesis.These findings suggested that KIF17 is involved in L.polyactis spermiogenesis.In particular,it may participate in nuclear shaping and tail formation by interacting with perinuclear microtubules during spermatid reshaping.In addition to providing evidence for the role of KIF17 in fish spermatid reshaping,this study provides important data for studies of reproductive biology in L.polyactis.展开更多
Epilepsy is a common and severe brain disease affecting>65 million people worldwide.Recent studies have shown that kinesin superfamily motor protein 17(KIF17)is expressed in neurons and is involved in regulating th...Epilepsy is a common and severe brain disease affecting>65 million people worldwide.Recent studies have shown that kinesin superfamily motor protein 17(KIF17)is expressed in neurons and is involved in regulating the dendrite-targeted transport of N-methyl-D-aspartate receptor subtype 2B(NR2B).However,the effect of KIF17 on epileptic seizures remains to be explored.We found that KIF17 was mainly expressed in neurons and that its expression was increased in epileptic brain tissue.In the kainic acid(KA)-induced epilepsy mouse model,KIF17 overexpression increased the severity of epileptic activity,whereas KIF17 knockdown had the opposite effect.In electrophysiological tests,KIF17 regulated excitatory synaptic transmission,potentially due to KIF17-mediated NR2B membrane expression.In addition,this report provides the first demonstration that KIF17 is modified by SUMOylation(SUMO,small ubiquitin-like modifier),which plays a vital role in the stabilization and maintenance of KIF17 in epilepsy.展开更多
基金Supported by the NSFC-Zhejiang Joint Fund for the Integration of Industrialization and Informatization(No.U1809212)the Scientific and Technical Project of Zhejiang Province(Nos.2021C02055,2017C02013)+1 种基金the National Natural Science Foundation of China(No.31272642)the Healthy Aquaculture,the K.C.Wong Magna Fund in Ningbo University,and the Collaborative Innovation Center for Zhejiang Marine High-efficiency。
文摘The homodimeric kinesin-2 protein KIF17 functions in intracellular transport and spermiogenesis in mammals.However,its role in fish spermiogenesis has not been reported.Here,we aimed to clone full-length kif17 cDNA and determine the molecular characteristics and expression patterns of KIF17 in Larimichthys polyactis spermiogenesis.The full-length cDNA of L.polyactis kif17(Lp-kif17)was sequenced and found to contain a 332-bp 5′untranslated region,480-bp 3′untranslated region,and 2433-bp open reading frame encoding 810 amino acids.Bioinformatics analyses showed that L.polyactis KIF17(Lp-KIF17)shared high sequence similarity with homologs in other animals and possessed an N-terminal motor domain with microtubule-binding sites and adenosine triphosphate(ATP)hydrolysis sites,a stalk domain containing two coiled-coil regions,and a C-terminal tail domain.The Lp-kif17 mRNA was widely expressed in various tissues,with the highest level in the brain,followed by that in the testis.Fluorescence in situ hybridization(FISH)analysis revealed that Lp-kif17 was continuously expressed in spermiogenesis,showing that it had potential functions in this process.Using immunofluorescence(IF)analysis,we found that Lp-KIF17 colocalized with tubulin and was transferred from the perinuclear cytoplasm to the side of spermatid where the tail forms during spermiogenesis.These findings suggested that KIF17 is involved in L.polyactis spermiogenesis.In particular,it may participate in nuclear shaping and tail formation by interacting with perinuclear microtubules during spermatid reshaping.In addition to providing evidence for the role of KIF17 in fish spermatid reshaping,this study provides important data for studies of reproductive biology in L.polyactis.
基金This work was supported by grants from the National Natural Science Foundation of China(81873788,81922023,82001378,and 82171440)the Chongqing Natural Science Foundation Project(cstc2019jcyj-msxmX0184)the Fifth Senior Medical Talents Program of Chongqing for Young and Middle-aged.
文摘Epilepsy is a common and severe brain disease affecting>65 million people worldwide.Recent studies have shown that kinesin superfamily motor protein 17(KIF17)is expressed in neurons and is involved in regulating the dendrite-targeted transport of N-methyl-D-aspartate receptor subtype 2B(NR2B).However,the effect of KIF17 on epileptic seizures remains to be explored.We found that KIF17 was mainly expressed in neurons and that its expression was increased in epileptic brain tissue.In the kainic acid(KA)-induced epilepsy mouse model,KIF17 overexpression increased the severity of epileptic activity,whereas KIF17 knockdown had the opposite effect.In electrophysiological tests,KIF17 regulated excitatory synaptic transmission,potentially due to KIF17-mediated NR2B membrane expression.In addition,this report provides the first demonstration that KIF17 is modified by SUMOylation(SUMO,small ubiquitin-like modifier),which plays a vital role in the stabilization and maintenance of KIF17 in epilepsy.
