Obesity is crucially involved in many metabolic diseases,such as type 2 diabetes,cardiovascular disease and cancer.Regulating the number or size of adipocytes has been suggested to be a potential treatment for obesity...Obesity is crucially involved in many metabolic diseases,such as type 2 diabetes,cardiovascular disease and cancer.Regulating the number or size of adipocytes has been suggested to be a potential treatment for obesity.In this study,we investigated the effect of pyrocincholic acid 3β-O-β-D-quinovopyranosyl-28-O-β-D-glucopyranoside(PAQG),a 27-nor-oleanolic acid saponin extracted from Metadina trichotoma,on adipogenesis and lipid metabolism in 3T3-L1 adipocytes.The 3T3-L1 pre-adipocytes were incubated with vehicle or PAQG for 6 days in differentiation process.PAQG significantly reduced the adipogenesis,adiponectin secretion and the expression level of key transcription factors related to adipogenesis,such as PPARc,C/EBPb,C/EBPa,and FABP4.Moreover,PAQG increased the levels of FFA and glycerol in medium and reduced TG level in mature adipocytes.Interestingly,PAQG not only promoted the activation of AMPK and genes involved in fatty oxidation including PDK4 and CPT1a,but also inhibited those genes involved in fatty acid biosynthesis,such as SREBP1c,FAS,ACCa and SCD1.In conclusion,PAQG inhibits the differentiation and regulates lipid metabolism of 3T3-L1 cells via AMPK pathway,suggesting that PAQG may be a novel and promising natural product for the treatment of obesity and hyperlipidemia.展开更多
A new flavan-3-ol glucoside, (-)-afzelechin-7-O-β-D-glucopyranoside (1), has been isolated from the stem of Daphniphyllum oldhami. The structure of the new compound was elucidated on the basis of detailed spectroscop...A new flavan-3-ol glucoside, (-)-afzelechin-7-O-β-D-glucopyranoside (1), has been isolated from the stem of Daphniphyllum oldhami. The structure of the new compound was elucidated on the basis of detailed spectroscopie analysis and comparison with related compounds.展开更多
Two new flavonoid glycosides were isolated from the flowering heads of Chrysanthemum morifolium. Their structures were determined to be luteolin 4＇-methoxy-7- O-（6＂-O-acetyl）-β-D-glucopyranoside （1） and acaceti...Two new flavonoid glycosides were isolated from the flowering heads of Chrysanthemum morifolium. Their structures were determined to be luteolin 4＇-methoxy-7- O-（6＂-O-acetyl）-β-D-glucopyranoside （1） and acacetin 7-O-（3＂-O-acetyl）-β-D-glucopyranoside （2） by means of 1H and 13C NMR spectroscopic analysis, including 2D NMR technique.展开更多
Two new spirostanol saponins, (25S)-spirostane-1β,3β,5β-triol 3-O-β-D-glucopyrano- side 1 and rhodeasapogenin 3-O-β-D-glucopyranosyl-(1 → 4)-β-D-glucopyranoside 2, together with a known saponin, rhodeasapogenin...Two new spirostanol saponins, (25S)-spirostane-1β,3β,5β-triol 3-O-β-D-glucopyrano- side 1 and rhodeasapogenin 3-O-β-D-glucopyranosyl-(1 → 4)-β-D-glucopyranoside 2, together with a known saponin, rhodeasapogenin 3-O-β-D-glucopyranoside 3, were isolated from the underground parts of Tupistra chinensis Bak.. Their structures were determined by spectroscopic analysis.展开更多
A new flavonoid glucoside with a known one was isolated from the leaves of Cassia angustifolia. Based on the spectral analysis, including MS, ^1H NMR, ^13C NMR, DEPT, ^1H-1HCOSY, HMQC, HMBC and NOSEY, their chemical s...A new flavonoid glucoside with a known one was isolated from the leaves of Cassia angustifolia. Based on the spectral analysis, including MS, ^1H NMR, ^13C NMR, DEPT, ^1H-1HCOSY, HMQC, HMBC and NOSEY, their chemical structures were determined as kaempferol-3-O-[（6＇＇＇-O-trans-sinnapoyt）-β-D-glucopyranosyl （1 → 6）]-β-D-glucopyranoside 1 and apigenin-6,8-di-C-glycoside 2.展开更多
A new polyacetylene glycoside,（1,3Z,11E）-tridecatriene-7,9-diyne-5-hydroxyl-6-O-β-D-glucopyranoside,was isolated from the rhizomes of Atractylodes lancea（Thunb.） DC.Its structure was elucidated on the basis of sp...A new polyacetylene glycoside,（1,3Z,11E）-tridecatriene-7,9-diyne-5-hydroxyl-6-O-β-D-glucopyranoside,was isolated from the rhizomes of Atractylodes lancea（Thunb.） DC.Its structure was elucidated on the basis of spectral analysis.展开更多
A new acylated flavonoid glycoside, 3-O-kaempferol-3-O-acetyl-6-O-(p-coumaroyl)-- D-glucopyranoside 1 was isolated from the whole plant of Anaphalis aureo-punctata. The structure was established by spectral methods.
Two new flavonol glycosides (1 and 2) together with two known flavonoides (3 and 4), were isolated from the whole plant of Knoxia corymbosa willd. The structures of 1 and 2 were elucidated as kaempferol-7-O-α-L-arabi...Two new flavonol glycosides (1 and 2) together with two known flavonoides (3 and 4), were isolated from the whole plant of Knoxia corymbosa willd. The structures of 1 and 2 were elucidated as kaempferol-7-O-α-L-arabinosyl-3-O-β-D-6'-acetylglucopyranoside and kaempferol -7-O-α-L-arabinosyl-3-O-β-D-3',6'-diacetylglucopyranoside respectively.展开更多
Delphinium brunonianum Royle belongs to Ranunculaceae family and has the effects of dispelling wind to relieve itching and cooling blood to detoxify.It was found that the extracts of D.brunonianum had good anticoagula...Delphinium brunonianum Royle belongs to Ranunculaceae family and has the effects of dispelling wind to relieve itching and cooling blood to detoxify.It was found that the extracts of D.brunonianum had good anticoagulant activity which was extracted with 70%ethanol in our previous researches.Then,16 compounds were isolated and identified from the extract of D.brunonianum,among which compounds 5,7-10,12,14,15-16 were isolated from this genus for the first time,and compounds 2-4 were isolated from this plant for the first time.And the coagulation activity assay showed that compounds 10,14 and 15 had good anticoagulant activity by activated partial thromboplastin time(APTT),thrombin time(TT)and prothrombin time(PT)in vitro.展开更多
对砂仁的化学成分进行研究,采用硅胶、葡聚糖凝胶LH-20、Rp-C18和MCI GEL CHP20P柱色谱,以及制备薄层色谱、HPLC等分离手段,从中分离得到8个萜类化合物,利用HR-ESI-MS、1D和2D NMR、IR、UV、[α]D和ECD等波谱技术鉴定为kravanhin A 3-O-...对砂仁的化学成分进行研究,采用硅胶、葡聚糖凝胶LH-20、Rp-C18和MCI GEL CHP20P柱色谱,以及制备薄层色谱、HPLC等分离手段,从中分离得到8个萜类化合物,利用HR-ESI-MS、1D和2D NMR、IR、UV、[α]D和ECD等波谱技术鉴定为kravanhin A 3-O-β-D-glucopyranoside (1)、kravanhin B (2)、6-eudesmene-1β,4β-diol (3)、oplodiol (4)、vicodiol (5)、(1R,2S,4R,7S)-vicodiol 9-O-β-D-glucopyranoside (6)、(1R,2S,4S,5R)-angelicoidenol 2-O-β-D-glucopyranoside (7)和(1S,2S,4R,6S)-bornane-2,6-diol 2-O-β-D-glucopyranoside (8)。其中化合物1为新化合物,化合物2~5为首次从砂仁中分离得到。采用STC-1细胞模型和2种酶学(GPa和PTP1B)模型对上述8个化合物进行降血糖活性测试,发现化合物1、7、8在浓度为25.0μmol·L-1时,对GLP-1的促泌率分别为692.8%、398.6%、483.3%;化合物6对GPa具有显著抑制活性,IC50为78.6μmol·L-1。展开更多
Objective： To investigate the chemical constituents from the roots of Angelica dahurica.Methods： The chemical constituents were isolated and purified by AB-8 macroporous adsorption resin and Sephadex LH-20 column as...Objective： To investigate the chemical constituents from the roots of Angelica dahurica.Methods： The chemical constituents were isolated and purified by AB-8 macroporous adsorption resin and Sephadex LH-20 column as well as semi-preparative reversed phase HPLC.The chemical structures were identified by spectral data.Results： Ten compounds were isolated and identified as xanthoarnol-3-O-β-d-glucopyranoside（1）,angedahuricoside A（2）,angedahuricoside B（3）,isofraxidin-7-O-β-d-glucopyranoside（4）,fraxidin-8-O-β-d-glucopyranoside（5）,（-）-marmesinin（6）,（2 S,3 R）-3-hydroxymarmesinin（7）,hyuganoside V（8）,daucosterol（9）,and sucrose（10）.Conclusion： Compounds 1 –3 are new ones and compounds 4 –6 and 8 are obtained from title plant for the first time.展开更多
Objective To study the constituents in the twigs and leaves of Rhododendron primulaeflorum. Methods The constituents were separated and purified with chromatographic methods. Their structures were elucidated by spectr...Objective To study the constituents in the twigs and leaves of Rhododendron primulaeflorum. Methods The constituents were separated and purified with chromatographic methods. Their structures were elucidated by spectroscopic methods (1D, 2D NMR, IR, and HR-ESI-MS) and chemical analyses. Results One new phenylpropanol glycoside, 4-hydroxyl-5-methoxyl-phenylpropanol-3-O-β-D-glucopyranoside (1), and its aglycone (2) were successfully isolated from the twigs and leaves of R. primulaeflorum. Conclusion Compound 1 is a new phenylpropanol glycoside. Compounds 1 and 2 are isolated from this plant for the first time.展开更多
文摘Obesity is crucially involved in many metabolic diseases,such as type 2 diabetes,cardiovascular disease and cancer.Regulating the number or size of adipocytes has been suggested to be a potential treatment for obesity.In this study,we investigated the effect of pyrocincholic acid 3β-O-β-D-quinovopyranosyl-28-O-β-D-glucopyranoside(PAQG),a 27-nor-oleanolic acid saponin extracted from Metadina trichotoma,on adipogenesis and lipid metabolism in 3T3-L1 adipocytes.The 3T3-L1 pre-adipocytes were incubated with vehicle or PAQG for 6 days in differentiation process.PAQG significantly reduced the adipogenesis,adiponectin secretion and the expression level of key transcription factors related to adipogenesis,such as PPARc,C/EBPb,C/EBPa,and FABP4.Moreover,PAQG increased the levels of FFA and glycerol in medium and reduced TG level in mature adipocytes.Interestingly,PAQG not only promoted the activation of AMPK and genes involved in fatty oxidation including PDK4 and CPT1a,but also inhibited those genes involved in fatty acid biosynthesis,such as SREBP1c,FAS,ACCa and SCD1.In conclusion,PAQG inhibits the differentiation and regulates lipid metabolism of 3T3-L1 cells via AMPK pathway,suggesting that PAQG may be a novel and promising natural product for the treatment of obesity and hyperlipidemia.
文摘A new flavan-3-ol glucoside, (-)-afzelechin-7-O-β-D-glucopyranoside (1), has been isolated from the stem of Daphniphyllum oldhami. The structure of the new compound was elucidated on the basis of detailed spectroscopie analysis and comparison with related compounds.
基金supported by the Natural Science Foundation of Jiangsu Science and Technology Department(No.BK2001219)
文摘Two new flavonoid glycosides were isolated from the flowering heads of Chrysanthemum morifolium. Their structures were determined to be luteolin 4＇-methoxy-7- O-（6＂-O-acetyl）-β-D-glucopyranoside （1） and acacetin 7-O-（3＂-O-acetyl）-β-D-glucopyranoside （2） by means of 1H and 13C NMR spectroscopic analysis, including 2D NMR technique.
文摘Two new spirostanol saponins, (25S)-spirostane-1β,3β,5β-triol 3-O-β-D-glucopyrano- side 1 and rhodeasapogenin 3-O-β-D-glucopyranosyl-(1 → 4)-β-D-glucopyranoside 2, together with a known saponin, rhodeasapogenin 3-O-β-D-glucopyranoside 3, were isolated from the underground parts of Tupistra chinensis Bak.. Their structures were determined by spectroscopic analysis.
文摘A new flavonoid glucoside with a known one was isolated from the leaves of Cassia angustifolia. Based on the spectral analysis, including MS, ^1H NMR, ^13C NMR, DEPT, ^1H-1HCOSY, HMQC, HMBC and NOSEY, their chemical structures were determined as kaempferol-3-O-[（6＇＇＇-O-trans-sinnapoyt）-β-D-glucopyranosyl （1 → 6）]-β-D-glucopyranoside 1 and apigenin-6,8-di-C-glycoside 2.
文摘A new polyacetylene glycoside,（1,3Z,11E）-tridecatriene-7,9-diyne-5-hydroxyl-6-O-β-D-glucopyranoside,was isolated from the rhizomes of Atractylodes lancea（Thunb.） DC.Its structure was elucidated on the basis of spectral analysis.
文摘A new acylated flavonoid glycoside, 3-O-kaempferol-3-O-acetyl-6-O-(p-coumaroyl)-- D-glucopyranoside 1 was isolated from the whole plant of Anaphalis aureo-punctata. The structure was established by spectral methods.
文摘Two new flavonol glycosides (1 and 2) together with two known flavonoides (3 and 4), were isolated from the whole plant of Knoxia corymbosa willd. The structures of 1 and 2 were elucidated as kaempferol-7-O-α-L-arabinosyl-3-O-β-D-6'-acetylglucopyranoside and kaempferol -7-O-α-L-arabinosyl-3-O-β-D-3',6'-diacetylglucopyranoside respectively.
基金This work was funded by Research on Precision Nutrition and Health Food,Department of Science and Technology of Henan Province(CXJD2021006).
文摘Delphinium brunonianum Royle belongs to Ranunculaceae family and has the effects of dispelling wind to relieve itching and cooling blood to detoxify.It was found that the extracts of D.brunonianum had good anticoagulant activity which was extracted with 70%ethanol in our previous researches.Then,16 compounds were isolated and identified from the extract of D.brunonianum,among which compounds 5,7-10,12,14,15-16 were isolated from this genus for the first time,and compounds 2-4 were isolated from this plant for the first time.And the coagulation activity assay showed that compounds 10,14 and 15 had good anticoagulant activity by activated partial thromboplastin time(APTT),thrombin time(TT)and prothrombin time(PT)in vitro.
基金The National Natural Science Foundation of China (81473321)Beijing Municipal Natural Science Foundation (7152086)+1 种基金the National Key Technology R & D Program of China (2011BAI07B082012BAI29B02)
文摘Objective： To investigate the chemical constituents from the roots of Angelica dahurica.Methods： The chemical constituents were isolated and purified by AB-8 macroporous adsorption resin and Sephadex LH-20 column as well as semi-preparative reversed phase HPLC.The chemical structures were identified by spectral data.Results： Ten compounds were isolated and identified as xanthoarnol-3-O-β-d-glucopyranoside（1）,angedahuricoside A（2）,angedahuricoside B（3）,isofraxidin-7-O-β-d-glucopyranoside（4）,fraxidin-8-O-β-d-glucopyranoside（5）,（-）-marmesinin（6）,（2 S,3 R）-3-hydroxymarmesinin（7）,hyuganoside V（8）,daucosterol（9）,and sucrose（10）.Conclusion： Compounds 1 –3 are new ones and compounds 4 –6 and 8 are obtained from title plant for the first time.
基金National Natural Science Foundation, China (20872122, 21142004)the New Century Talents Scheme of the Ministry of Education, China(NECT-08-0820)+1 种基金the Fundamental Research Funds for the Central Universities, China (SWJTU2010ZT09)SWJTU Science Research Funds (2008A17)
文摘Objective To study the constituents in the twigs and leaves of Rhododendron primulaeflorum. Methods The constituents were separated and purified with chromatographic methods. Their structures were elucidated by spectroscopic methods (1D, 2D NMR, IR, and HR-ESI-MS) and chemical analyses. Results One new phenylpropanol glycoside, 4-hydroxyl-5-methoxyl-phenylpropanol-3-O-β-D-glucopyranoside (1), and its aglycone (2) were successfully isolated from the twigs and leaves of R. primulaeflorum. Conclusion Compound 1 is a new phenylpropanol glycoside. Compounds 1 and 2 are isolated from this plant for the first time.
