利用生物学方法和气相色谱法分别对柿有代表性的早、中、晚熟品种果实中内源赤霉素类物质(GAs)活性及脱落酸(ABA)含量的变化进行了测定。GAs 活性和 ABA 含量在幼果初期都很高,以后随果实的发育而迅速降低,在果实生长速度缓慢的第二期...利用生物学方法和气相色谱法分别对柿有代表性的早、中、晚熟品种果实中内源赤霉素类物质(GAs)活性及脱落酸(ABA)含量的变化进行了测定。GAs 活性和 ABA 含量在幼果初期都很高,以后随果实的发育而迅速降低,在果实生长速度缓慢的第二期变化不大。但自果实进入成熟期开始,GAs 活性再次迅速降低,而 ABA 含量却呈增加趋势。发生这种变化的时期,与果实的成熟期一致。讨论了内源值物生长调节物质的平衡与果实成熟的关系。展开更多
Objective:To explore the effect of Diospyros kaki on cattle spermatozoa during chilling and cryopreservation.Methods: Five milliliter of blended Persimmon (Diospyros kaki) flesh was added to 45 mL TCF to obtain 10% st...Objective:To explore the effect of Diospyros kaki on cattle spermatozoa during chilling and cryopreservation.Methods: Five milliliter of blended Persimmon (Diospyros kaki) flesh was added to 45 mL TCF to obtain 10% stock solution. Kaki enriched extender (KEE) was prepared by adding to TCF in concentrations 0.0/5.0 mL (control, 0%), 0.5/4.5 mL (1%), 1/4 mL (2%), 1.5/3.5 mL (3%), 2.0/3.0 mL (4%), 2.5/2.5 mL (5%), 3.0/2.0 mL (6%), 3.5/1.5 mL (7%), 4.0/1.0 mL (8%), 4.5/0.5 mL (9%) and 5.0/0.0 mL (10%) to obtain a final volume 5 mL in each tube. Whole egg yolk was added to each tube to obtain KEE with 20% egg yolk (KEEY), all tubes were centrifuged to get rid of debris. Semen was added to the supernatants in other tubes. Extended semen was subjected to evaluation (motility, alive sperm and intact sperm membrane (HOST) %) in both chilled and cryopreserved semen. Conception rate was carried out.Results:Sperm motility was significantly (P<0.0001) kept high after 11 d of chilling with the concentration 1%, 2%, 3%, 4%, 5% as compared to the control (41.67±1.67, 41.67±1.67, 40.00±0.00, 41.67±1.67 and 41.67±1.67, respectively) and also non-significantly kept high at the other concentrations up to 9 d of chilling. Addition of KEE had significantly (P<0.0033) improved post thawing sperm motility % with the concentrations 1, 2, 3, 4, 5 and 6% as compared to the control (51.67±5.27, 55.00±3.16, 48.33±1.05, 45.00±3.96, 57.00±2.50, 55.00±5.00 and 43.33±5.11 respectively).While the other concentrations exhibit no effect. Addition of KEE maintained alive sperm%, abnormalities% and % of intact spermatozoa membranes (HOST %) as good as the control with all concentrations of kaki used in our study. The conception rate upon using frozen semen in insemination showed higher conception rate in concentrations of 2%, 4% and 6 % KEE in cattle.Conclusion: It could be concluded that some concentrations ofDiospyros kaki improved bull semen quality post-chilling and post-freezing.展开更多
Objective In order to analyze the active components in the extracts from Kaki Folium(KF),quantitative metabolomics approach was adopted to investigate the number of active components existing among the different extra...Objective In order to analyze the active components in the extracts from Kaki Folium(KF),quantitative metabolomics approach was adopted to investigate the number of active components existing among the different extracts and their variation.Methods LC-MS method was established for the quantitative determination of the active components taking the mixture with reference substance as tested sample.Results In terms of the number of active components and amount presented in the different tested samples of KF extracted by many types of solvents,variation was observed.But rutin,astragalin,and kaempferol were presented in all samples.Difference was found between the samples extracted from the products on market and from the raw materials of KF processed by polar solvents with different recipes.However,the three active components were found in all samples examined.Conclusion These results might be valuable as all information and could be used for the optimization of raw materials extraction procedure to enhance the productivity.展开更多
为了确定罗田甜柿(Diospyros kaki Thunb.)成熟果实中总三萜含量和提取条件,以湖北省罗田县的罗田甜柿果实为试材,从不同的溶剂、乙醇体积分数、提取时间、温度、料液比、提取次数来确定提取罗田甜柿成熟果实中总三萜的最佳条件,结果表...为了确定罗田甜柿(Diospyros kaki Thunb.)成熟果实中总三萜含量和提取条件,以湖北省罗田县的罗田甜柿果实为试材,从不同的溶剂、乙醇体积分数、提取时间、温度、料液比、提取次数来确定提取罗田甜柿成熟果实中总三萜的最佳条件,结果表明,通过单因素试验确定提取条件为70%乙醇作为溶剂,提取时间3 h,温度85℃,料液比1∶11(m∶V,g∶m L),提取2次,总三萜提取量最高可达51.13 mg/100 g(FW)。展开更多
以宝华甜柿(Diospyros kaki c.v.‘Baohuatianshi’)为材料,在对试验材料进行热激处理后,检测了活性氧代谢相关生理指标的变化情况。结果表明,经过热激处理后,与对照相比,外植体内超氧化物歧化酶(SOD)和过氧化氢酶(CAT)活力出现了提高,...以宝华甜柿(Diospyros kaki c.v.‘Baohuatianshi’)为材料,在对试验材料进行热激处理后,检测了活性氧代谢相关生理指标的变化情况。结果表明,经过热激处理后,与对照相比,外植体内超氧化物歧化酶(SOD)和过氧化氢酶(CAT)活力出现了提高,丙二醛(MDA)和过氧化氢(H2O2)的含量则有所降低。说明热激处理能够降低活性氧含量,保护了细胞膜结构的完整性,抑制了褐变发生。展开更多
随着国内外市场经济的发展和人民生活水平的提高,柿(Diospyros kaki L.)作为绿色功能保健型果品,其社会需求量日益增大。因此,了解柿果实脱涩机理及其易软化、难贮藏特征,对其脱涩、贮藏保鲜技术进行研究显得尤为必要。综述了柿果实脱...随着国内外市场经济的发展和人民生活水平的提高,柿(Diospyros kaki L.)作为绿色功能保健型果品,其社会需求量日益增大。因此,了解柿果实脱涩机理及其易软化、难贮藏特征,对其脱涩、贮藏保鲜技术进行研究显得尤为必要。综述了柿果实脱涩机理及方法、贮藏保鲜技术相关研究报道,并根据研究现状展望了未来的发展方向和趋势。展开更多
文摘利用生物学方法和气相色谱法分别对柿有代表性的早、中、晚熟品种果实中内源赤霉素类物质(GAs)活性及脱落酸(ABA)含量的变化进行了测定。GAs 活性和 ABA 含量在幼果初期都很高,以后随果实的发育而迅速降低,在果实生长速度缓慢的第二期变化不大。但自果实进入成熟期开始,GAs 活性再次迅速降低,而 ABA 含量却呈增加趋势。发生这种变化的时期,与果实的成熟期一致。讨论了内源值物生长调节物质的平衡与果实成熟的关系。
文摘Objective:To explore the effect of Diospyros kaki on cattle spermatozoa during chilling and cryopreservation.Methods: Five milliliter of blended Persimmon (Diospyros kaki) flesh was added to 45 mL TCF to obtain 10% stock solution. Kaki enriched extender (KEE) was prepared by adding to TCF in concentrations 0.0/5.0 mL (control, 0%), 0.5/4.5 mL (1%), 1/4 mL (2%), 1.5/3.5 mL (3%), 2.0/3.0 mL (4%), 2.5/2.5 mL (5%), 3.0/2.0 mL (6%), 3.5/1.5 mL (7%), 4.0/1.0 mL (8%), 4.5/0.5 mL (9%) and 5.0/0.0 mL (10%) to obtain a final volume 5 mL in each tube. Whole egg yolk was added to each tube to obtain KEE with 20% egg yolk (KEEY), all tubes were centrifuged to get rid of debris. Semen was added to the supernatants in other tubes. Extended semen was subjected to evaluation (motility, alive sperm and intact sperm membrane (HOST) %) in both chilled and cryopreserved semen. Conception rate was carried out.Results:Sperm motility was significantly (P<0.0001) kept high after 11 d of chilling with the concentration 1%, 2%, 3%, 4%, 5% as compared to the control (41.67±1.67, 41.67±1.67, 40.00±0.00, 41.67±1.67 and 41.67±1.67, respectively) and also non-significantly kept high at the other concentrations up to 9 d of chilling. Addition of KEE had significantly (P<0.0033) improved post thawing sperm motility % with the concentrations 1, 2, 3, 4, 5 and 6% as compared to the control (51.67±5.27, 55.00±3.16, 48.33±1.05, 45.00±3.96, 57.00±2.50, 55.00±5.00 and 43.33±5.11 respectively).While the other concentrations exhibit no effect. Addition of KEE maintained alive sperm%, abnormalities% and % of intact spermatozoa membranes (HOST %) as good as the control with all concentrations of kaki used in our study. The conception rate upon using frozen semen in insemination showed higher conception rate in concentrations of 2%, 4% and 6 % KEE in cattle.Conclusion: It could be concluded that some concentrations ofDiospyros kaki improved bull semen quality post-chilling and post-freezing.
基金National Basic Research Plan (973 Plan) Foundation of China (2010CB735602)National Innovation Plan for New Drugs (2011ZX09304-002)
文摘Objective In order to analyze the active components in the extracts from Kaki Folium(KF),quantitative metabolomics approach was adopted to investigate the number of active components existing among the different extracts and their variation.Methods LC-MS method was established for the quantitative determination of the active components taking the mixture with reference substance as tested sample.Results In terms of the number of active components and amount presented in the different tested samples of KF extracted by many types of solvents,variation was observed.But rutin,astragalin,and kaempferol were presented in all samples.Difference was found between the samples extracted from the products on market and from the raw materials of KF processed by polar solvents with different recipes.However,the three active components were found in all samples examined.Conclusion These results might be valuable as all information and could be used for the optimization of raw materials extraction procedure to enhance the productivity.
文摘以宝华甜柿(Diospyros kaki c.v.‘Baohuatianshi’)为材料,在对试验材料进行热激处理后,检测了活性氧代谢相关生理指标的变化情况。结果表明,经过热激处理后,与对照相比,外植体内超氧化物歧化酶(SOD)和过氧化氢酶(CAT)活力出现了提高,丙二醛(MDA)和过氧化氢(H2O2)的含量则有所降低。说明热激处理能够降低活性氧含量,保护了细胞膜结构的完整性,抑制了褐变发生。
文摘随着国内外市场经济的发展和人民生活水平的提高,柿(Diospyros kaki L.)作为绿色功能保健型果品,其社会需求量日益增大。因此,了解柿果实脱涩机理及其易软化、难贮藏特征,对其脱涩、贮藏保鲜技术进行研究显得尤为必要。综述了柿果实脱涩机理及方法、贮藏保鲜技术相关研究报道,并根据研究现状展望了未来的发展方向和趋势。
