Study on the clinical effect and safety of Kangfuxin liquid combined with microneedling in the treatment of facial skin photoaging

Background and Objectives:Microneedling has been introduced as a new technique to address the growing concern of facial skin photoaging.Kangfuxin liquid has been found to promote the process of skin wound repair,including reducing inflammatory response,improving immunity and enhancing antioxidant levels.In this prospective randomized double-blinded study,we wanted to explore the clinical efficacy and safety of Kangfuxin liquid combined with microneedling in treating facial skin photoaging.Methods:57 patients with facial skin photoaging were randomly divided into two groups.The treatment group(28 cases)received microneedle therapy with Kangfuxin liquid,while the control group(29 cases)received microneedle therapy with physiological saline.The treatment interval was 4 weeks,and a total of 3 treatments were performed.Compare the VISIA scores of facial photoaging features such as wrinkles,texture,pores,spots and ultraviolet pigmentation between two groups of patients before and after treatment,Global Score for Photoaging,satisfaction evaluation,and record the occurrence of adverse reactions.Results:After treatment,the treatment group showed more significant improvement in wrinkles,texture,pores,spots and ultraviolet pigmentation,and the Global Score for Photoaging was better than the control group(P<0.05).The satisfaction rate with improving skin in the treatment group was 85.71%,which was higher than 75.86%in the control group(P<0.05).Both groups did not experience adverse reactions such as skin infection,pigmentation or hypopigmentation,scar formation,or worsening of melasma.Conclusion:Kangfuxin liquid combined with microneedling therapy has a good improvement effect on facial skin photoaging,with a low incidence of adverse reactions and high patient satisfaction.It is worthy of clinical promotion.

Effects of Kangfuxin Solution (康复新液) Combined with Low-level Laser on Cytokines and Immune Function in Patients with Radiation-induced Oral Mucositis

Objective:To investigate the effect of Kangfuxin Solution(康复新液)combined with low-level laser therapy(LLLT)on cytokines and immune function in patients with radiation-induced oral mucositis.Methods:There were 84 nasopharyngeal carcinoma patients with oral mucositis after intensity-modulated radiation therapy(IMRT)were randomly divided into the control group(CG)and the observation group(OG),with 42 cases in each group.The CG was given LLLT,and the OG was treated with Kangfuxin Solution in addition to LLLT for 10 consecutive days.The healing time of oral mucosa,mucositis grading,oral pain scores,cytokines(interleukin-6,interleukin-1βand tumor necrosis factor-α)and T lymphocyte subsets were compared between the 2 groups before and after treatment.Results:The healing time of oral mucosa in the OG was(6.8±1.4)d,which was significantly shorter than(8.6±1.9)d in the CG(t=4.943,P<0.01).After treatment,the grading of oral mucositis in the OG was better than that in the CG,with a statistically significant difference(Z=2.942,P<0.05).The oral pain scores of the OG was lower than that in the CG at different time points after treatment,and the difference was statistically significant(t=8.207,11.017,P<0.01).After treatment,the levels of IL-6,IL-1βand TNF-αin peripheral blood of the OG were significantly lower than those in the CG(t=5.217,2.775,4.053,P<0.01).There were statistically significant differences in CD4+,CD8+and CD4+/CD8+between the OG and the CG after treatment(t=5.692,6.093,3.658,P<0.01).Conclusion:Kangfuxin Solution combined with LLLT can significantly shorten the healing time of oral mucosal,reduce the grading of oral mucositis,relieve oral pain,reduce inflammatory response and improve the immune function of patients.

Kangfuxin Fluid on the Treatment of Ulcerative Colitis with Retention Enema： a Systematic Review

Objective： To systematically evaluate the effectiveness of Kangfuxin Fluid （KFX） in Ulcerative Colitis （UC） with retention enema. Methods： We searched The Cochanrane Library, Pubmed, EMbase, VIP, CNKI, CBM and WanFang Data from inception to December.2017, and retrospectively incorporated into the literature reference. And a systematic review of randomized controlled trials was performed. Meta-analysis was performed with RevMan5.3 software. Results： A total of 18 randomized controlled trials and 1417 patients were enrolled, among which 731 cases were in experimental group and 686 cases were in control group. The meta-analysis results revealed that the effectiveness of KFX on UC with retention enema was significantly superior to the controlled treatment （RR=1.22 95%CI： [1.16,1.27], Z=8.14, p〈0.00001）. And, the score of quality of life was significantly increased after KFX treatment （MD=11.12, 95% CI [7.00, 15.23], Z=5.30, p〈0.00001）. Conclusion： KFX on the treatment of UC with retention enema is potentially effective to improve patient＇s quality of life, and it is worth being popularized clinically. Depth researches and high quality randomized controlled trials on the efficacy of UC are necessary.

Effect of Kangfuxin solution on inflammatory, immune, oxidative indexes and vascular factors in patients with ulcerative colitis

Objective:To explore the effect of Kangfuxin solution on inflammatory, immune, oxidative indexes and vascular factorsin patients with ulcerative colitis.Methods: 172 cases of ulcerative colitis from August 2015 to February 2017 were selected and divided into control group (n=86) and observation group (n=86) according to random principle, the control group was treated with Mesalazin Enteric-coated Tablets, and the observation group was treated with enema by infusion of rectal drip on the basis of the control group, and the levels of the two groups of inflammatory factors, immune indexes, oxidation indexes and vascular factors were compared.Results: After treatment, the total effective rate of the observation group (88.37%) was significantly higher than that of the control group (73.26%) (P<0.05);the two groups of CRP and TNF-α were significantly lower than those before treatment (P<0.05), and the observation group was significantly lower than that of the control group (P<0.05);the two groups of CD4+, CD4+/CD8+ and SOD increased significantly compared with those before treatment (P<0.05), while CD8+, MDA and LOP were significantly lower than those before treatment (P<0.05), and the CD4+, CD4+/CD8+ and SOD in the observation group were significantly higher than those in the control group (P<0.05), while CD8+, MDA and LOP were significantly lower than those in the control group (P<0.05);The levels of VEGF and PDGF in both groups were significantly higher than those before treatment (P<0.05), and the levels of VEGF and PDG in observation group were significantly higher than those in control group (P<0.05).Conclusions:The combined treatment of Kangfuxin Liquid and mesalazine can significantly improve the clinical efficacy of the patients. The mechanism of its action is related to reducing the inflammatory response and enhancing the immune, antioxidant functionsand vascular factors. It is worthy of further study in clinical.

Clinical observation and experimental study on Kangfuxin fluid in treating indwelling needle-related phlebitis

Objective To investigate the therapeutic effect of Kangfuxin Fluid on indwelling needle-related phlebitis by observation of clinical samples and pharmacological study.Methods Eighty-five patients with indwelling needle-related phlebitis in the neurosurgery department of a Grade A hospital in Shanxi Province from June 2019 to November 2021 were randomly divided into the study group(43 cases)and the vehicle group(42 cases).Patients in the study group were treated with KFX,and patients in the control group were treated with hydrocolloid dressing.The time for symptoms(such as redness and pain)to disappear was recorded.The effect of tissue repair was evaluated by the fin crosscutting model and the anti-inflammatory effect was evaluated by the inflammatory models.Q-PCR assay was used to detect the mRNA expression levels of IL-6 and TNF-αin LPS-stimulated zebrafish.The effect on promoting angiogenesis was evaluated by the inter-segmental blood vascular defect model of zebrafish.Results The curative effect of the study group was better than the control group.The pain resolution time and the swelling resolution time in the study group were(1.67±0.90)d and(2.25±0.92)d,respectively,shorter than those in the control group(P<0.05).KFX can improve the ISV index,increase the regeneration length of the fin,inhibit the migration of neutrophils in three inflammatory models and reduce the mRNA expression levels of IL-6 and TNF-αin LPS-stimulated zebrafish.Conclusion KFX exerts great clinical efficacy in the treatment of indwelling needle-induced phlebitis which may be associated with inflammation inhibition,angiogenesis promotion,and tissue repair.

AQC柱前衍生高效液相色谱方法测定康复新液中游离氨基酸和总肽的含量

目的应用6-氨基喹啉基-N-羟基琥珀酰亚氨基氨基甲酸酯(AQC)柱前衍生化技术,建立康复新液中游离氨基酸和总肽的高效液相色谱(HPLC)含量测定方法,并以总肽含量为指标对市售样品进行检测。方法以十八烷基硅烷键合硅胶为填充剂(Kromasil 100-5 C18色谱柱);60%乙腈(A)-0.14 mol·L^(-1)三水合乙酸钠溶液,用磷酸调节pH值至5.0(B)为流动相,梯度洗脱;柱温:39℃;流速:1.0 mL·min^(-1),检测波长:248 nm。结果14种氨基酸均能达到良好的分离效果;门冬氨酸、谷氨酸、丝氨酸、组氨酸、甘氨酸、精氨酸、苏氨酸、丙氨酸、脯氨酸、缬氨酸、赖氨酸、异亮氨酸、亮氨酸和苯丙氨酸分别在2.0~99.7、3.4~168.5、2.8~139.6、4.0~201.4、4.8~238.1、6.7~336.9、3.3~167.5、9.7~487.3、4.1~202.5、4.4~221.6、5.4~270.5、3.3~166.8、4.8~240.8、4.7~236.6μg·mL^(-1)范围内呈现良好的线性关系(r=0.9995~1.0000);游离氨基酸的平均加样回收率(n=6)为86.8%~108.1%,RSD为2.8%~4.4%,总肽酸水解氨基酸的平均加样回收率(n=6)为83.2%~102.7%,RSD为0.1%~3.1%;仪器精密度、重复性、稳定性实验的RSD均小于5.0%。结论该方法与现行质量标准的紫外分光光度法比较,二者测定的总氨基酸含量结果基本一致,但前者的专属性、重现性更优;以具有生物活性的总肽为质控指标,针对性更强,可为康复新液的质量评价提供更科学、合理的方法。

康复新液联合沙利度胺防治鼻咽癌患者放射性口腔黏膜炎的临床效果

目的 探究康复新液联合沙利度胺预防鼻咽癌(NPC)患者放射性口腔黏膜炎(ROM)的临床效果。方法选取2021—2022年就诊于南昌大学第二附属医院的NPC患者60例,采用随机数字表法分为试验组和对照组,各30例。对照组自放疗开始,每日使用复方漱口液含漱和沙利度胺片口服,试验组在对照组基础上加用康复新液,2组均治疗至放疗结束。比较2组ROM发生情况、放射口腔受量、客观缓解率及不良反应的发生情况。结果 第4、6、8周时,试验组ROM的发生率显著低于对照组(P<0.05),且整个放疗期间试验组3、4级ROM的发生率也低于对照组(P<0.05)。2组口腔照射剂量体积的平均剂量、V30、V40、V50、V55、客观缓解率及不良反应发生率比较,差异均无统计学意义(P>0.05)。结论 康复新液联合沙利度胺可降低NPC患者ROM的发生率并减轻其严重程度。

康复新液在消化内镜术后患者中应用效果和安全性的Meta分析

目的系统评价康复新液联合常规治疗在消化内镜术后患者中的应用效果与安全性。方法计算机检索PubMed、Embase、Cochrane Library、CNKI、WanFang Data、VIP数据库,搜集关于消化内镜术后应用康复新液的随机对照试验(RCT),检索时限均从建库至2023年12月31日,由2名研究者独立筛选文献、提取数据并评价纳入研究的偏倚风险后,使用RevMan 5.3和Stata 14软件进行Meta分析。结果共纳入14个RCT,包括1359例患者。Meta分析结果显示,与常规治疗组相比,康复新液联用组可以提高术后溃疡完全愈合率[RR=1.32,95%CI(1.07,1.61),P=0.009]、术后临床症状缓解率[RR=1.20,95%CI(1.13,1.27),P<0.001],但2组的不良反应发生率差异无统计学意义(P>0.05)。亚组分析显示,康复新液联用组术后2周的溃疡完全愈合率高于常规治疗组[RR=1.56,95%CI(1.28,1.92),P<0.001],但2组术后4周的溃疡完全愈合率差异无统计学意义(P>0.05);康复新液口服给药组[RR=1.19,95%CI(1.11,1.27),P<0.001]或经内镜外用喷洒给药组[RR=1.23,95%CI(1.09,1.39),P<0.001]的临床症状缓解率均高于常规治疗组。结论消化内镜术后常规用药基础上联合康复新液治疗可进一步提高患者的临床疗效,尤其是促进术后2周内溃疡完全愈合、缓解术后临床症状方面效果较好,但能否降低药品不良反应发生率的证据不足。受纳入研究数量和质量的限制,上述结论尚待更多高质量研究予以验证。

康复新漱口液辅助治疗对牙周炎合并糖尿病患者口腔菌群和龈沟液相关因子的影响

目的:探讨康复新漱口液辅助治疗对牙周炎合并糖尿病患者口腔菌群和龈沟液相关因子的影响。方法:选取2022年6月-2022年10月于笔者医院接受治疗的98例牙周炎合并糖尿病患者为研究对象,按照随机数字表法将其分为对照组和观察组,每组49例。所有患者均接受牙周基础治疗,在此基础上对照组给予双氧水治疗,观察组在对照组基础上采用康复新漱口液辅助治疗,两组均治疗4周。比较两组患者临床治疗效果及治疗前后牙周相关指标。结果:观察组总有效率为91.84%,高于对照组的77.55%(P<0.05);治疗后,两组PLI、PD、SBI和AL均降低(P<0.05),且观察组均低于对照组(P<0.05);治疗后,观察组Td、Pi、Tf和Pg均低于对照组和治疗前(P<0.05);治疗后,两组TNF-α、IL-6、hs-CRP均降低(P<0.05),且观察组均低于对照组(P<0.05)。结论:给予牙周炎合并糖尿病患者康复新漱口液辅助治疗可改善患者牙周状态,降低口腔菌群水平,减轻炎性状态。

Analysis and monitoring of drug therapy in a patient with peptic ulcer complicated by infection:A case report

BACKGROUND The role of primary-level medical pharmacists in medical institutions in China is limited;therefore,it is necessary to explore the role of pharmacists in the process of drug treatment.CASE SUMMARY A Chinese pharmacist participated in the complete treatment of a patient with a duodenal ulcer.The rationale for drug treatment was evaluated,and adjustments were made to the antacid and anti-infective regimen,as well as the dose and frequency of administration.Body temperature,routine blood examination,and adverse drug reactions were strictly monitored.During treatment,the pharmacist recommended anti-infective therapy with ampicillin-sulbactam,which effectively controlled the infection.Additionally,the pharmacist suggested changing famotidine to lansoprazole for acid suppression and gastroprotective treatment,combined with Chinese patent medicine such as Kangfuxin Liquid.This is the first case report of a pharmacist in primary-level medical institutions adjusting drug use for patients with duodenal ulcer and pulmonary infection.CONCLUSION A pharmacist participated in the treatment process,provided individualized medication adjustment,and achieved good clinical results.

大柴胡汤联合短波紫外线与康复新液治疗小儿溃疡性口腔炎疗效观察

目的:观察大柴胡汤联合短波紫外线与康复新液治疗小儿溃疡性口腔炎的临床疗效。方法:将92例溃疡性口腔炎患儿采用抽签法分为对照组和研究组,各46例.对照组予短波紫外线联合康复新液治疗,研究组在对照组基础上予大柴胡汤治疗。比较两组患者临床疗效、中医证候积分、相关症状改善情况及血清C反应蛋白(C-reaction protein,CRP)、白细胞介素6(interleukin-6,IL-6)、IL-10、肿瘤坏死因子α(tumor necrosis factor α,TNF-α)表达水平及不良反应发生情况。结果:研究组总有效率为93.5%(43/46),高于对照组的80.4%(37/46)(P<0.05);中医证候积分低于对照组(P<0.05);相关症状改善优于对照组(P<0.05);血清CRP、IL-6、IL-10、TNF-α表达均低于对照组(P<0.05);患者潮热及口苦不良反应发生率高于对照组(P<0.05),但总的不良反应发生率两组比较差异无统计学意义(P>0.05)。结论:与单纯短波紫外线联合康复新液治疗比较,大柴胡汤联合短波紫外线与康复新液治疗小儿溃疡性口腔炎疗效更优,能够改善中医证候及症状,降低血清CRP、IL-6、IL-10、TNF-α表达水平,安全性好。

康复新液联合重组人干扰素α-2b治疗慢性宫颈炎的效果

目的分析康复新液联合重组人干扰素α-2b治疗慢性宫颈炎的效果。方法随机抽取平顶山第一人民医院2019年1月至2022年12月收治的800例慢性宫颈炎患者,采用摸红蓝球法分为联合组(400例)与单一组(400例)。单一组接受重组人干扰素α-2b,联合组在单一组基础上接受康复新液。对比两组疗效、临床症状缓解时间(阴道分泌物增多、外阴瘙痒、性交后出血)、宫颈修复时间、不良反应发生率、生活质量。结果联合组总有效率[96.75%(387/400)]较单一组[86.50%(346/400)]高(P<0.05)。与单一组比,联合组阴道分泌物增多、外阴瘙痒、性交后出血缓解时间及宫颈修复时间较短(P<0.05)。两组不良反应发生率差异无统计学意义(P>0.05)。治疗后与单一组比,联合组的患者生活质量评分较高(P<0.05)。结论采用康复新液、重组人干扰素α-2b联合治疗慢性宫颈炎,可改善临床症状,提高生活质量,临床效果和安全性确切。

康复新液联合rb-bFGF凝胶对放射性皮炎患者皮肤恢复情况及炎性反应的影响

目的探究康复新液联合牛碱性成纤维细胞生长因子(rb-bFGF)凝胶治疗放射性皮炎的效果。方法选取2020年3月—2023年3月荣成市人民医院收治的96例放射性皮炎患者为研究对象,按随机数字表法将其分为对照组(n=48)和观察组(n=48)。对照组采用rb-bFGF凝胶治疗,观察组采用康复新液联合rb-bFGF凝胶治疗。比较两组患者的临床疗效、炎性反应、皮肤恢复情况及不良反应发生情况。结果观察组的治疗总有效率为93.75%,高于对照组的79.17%,差异有统计学意义(P<0.05)。治疗后,观察组的白细胞介素-6、C反应蛋白水平均低于对照组,组间差异有统计学意义(P<0.05)。观察组各项症状消失时间均短于对照组,组间差异有统计学意义(P<0.05)。两组的不良反应发生率比较,差异无统计学意义(P>0.05)。结论康复新液联合rb-bFGF凝胶治疗放射性皮炎的效果确切,可减轻患者的炎性反应,促进其皮肤愈合,且安全性较好。

止痛如神汤加减方联合康复新液灌肠治疗气滞血瘀型混合痔术后肛门疼痛的临床效果

目的:观察止痛如神汤加减方联合康复新液灌肠治疗气滞血瘀型混合痔术后肛门疼痛的临床效果。方法:选择2021年1月—2022年12月在崇州市人民医院收治的100例混合痔患者,按照随机数字表法分为A组、B组、C组三组。A组30例,予常规西医治疗;B组35例,在A组基础上予康复新液灌肠治疗;C组35例,在B组基础上,予止痛如神汤加减方治疗。三组术后1 d、7 d均评价创面水肿、创面肉芽组织生长情况,通过数字疼痛评分法(NRS)评价肛门疼痛情况,记录三组患者肛门水肿消退时间、创面分泌物消失时间、创面愈合时间,检测三组肿瘤坏死因子α(TNF-α)、表皮生长因子(EGF)、β-内啡肽(β-EP)、白细胞介素-22(IL-22)、纤维连接蛋白(FN)、血管内皮生长因子(VEGF)水平,术前、术后1个月测量患者直肠肛管抑制反射阈值、肛管静息压、肛管高压带长度。结果:C组创面水肿、NRS评分较A组、B组低(P<0.05),创面肉芽组织生长评分较A组、B组高(P<0.05);C组创面分泌物消失时间、肛门水肿消退时间、创面愈合时间较A组、B组短(P<0.05);C组直肠肛管抑制反射阈值、肛管静息压较A组、B组高(P<0.05),C组肛管高压带长度较A组、B组短(P<0.05);C组TNF-α、IL-22水平较A组、B组低(P<0.05),C组β-EP、EGF、FN、VEGF水平较A组、B组高(P<0.05)。结论:止痛如神汤加减方联合康复新液灌肠可抑制炎症因子水平,调节疼痛介质及EGF、FN、VEGF指标,缓解肛门疼痛,促进创面愈合,提升肛门功能。

康复新液联合PPI治疗反流性食管炎的疗效及安全性Meta分析

目的纳入系统评价康复新液联合质子泵抑制剂(Proton pump inhibitor,PPI)治疗反流性食管炎(Reflux esophagitis,RE)相较于单独使用PPI的疗效和安全性评价。方法以康复新液、反流性食管炎、反流性食道炎、KangFuXin、reflux esophagitis等为检索词在CNKI、WanFang Data、VIP、Embase、Web of Science、PubMed和The Cochrane Library数据库检索康复新液联合PPI治疗RE的随机对照研究,采用RevMan 5.4软件进行Meta分析。结果共纳入25项研究,统计分析结果显示康复新液联合PPI治疗RE较单独PPI治疗能显著提高临床有效率(95%CI:1.15,1.24)、胃镜下有效率(95%CI:1.14,1.22),血清胃泌素(95%CI:1.15,2.03)和胃动素的分泌(95%CI:0.96,1.39),降低复发率(95%CI:0.20,0.61),且不会增高不良反应发生率(95%CI:0.33~0.79),此外,不同PPI种类以及患者年龄高低对临床有效率无显著影响,而不同内镜下诊断标准对内镜下有效率也无显著影响。结论康复新液联合PPI治疗RE较单独PPI治疗能取得更好的疗效且减少疾病复发,并不会额外造成不良反应的发生。

康复新液加胆石利通片联合莫沙必利治疗胆囊切除术后胆汁反流性胃炎的临床疗效研究

目的探讨康复新液加胆石利通片联合莫沙必利治疗胆囊切除术后胆汁反流性胃炎(BRG)的临床疗效。方法选择2022年10月至2023年10月在九江市第一人民医院行胆囊切除术治疗且术后出现BRG的60例患者作为研究对象,根据随机数字表法分为试验组(n=30)和对照组(n=30)。对照组患者接受胆石利通片联合莫沙必利治疗,试验组患者在对照组基础上加用康复新液治疗,两组均连续治疗4周。比较两组的临床疗效、临床症状积分、24 h内胆汁反流情况、实验室指标。结果试验组的总有效率高于对照组,差异有统计学意义(P<0.05)。治疗后,两组患者的临床症状积分低于本组治疗前,且试验组的临床症状积分低于对照组,差异有统计学意义(P<0.05)。治疗后,两组患者的24 h内最长反流时间均短于本组治疗前,反流次数与反流时间>5 min次数均少于本组治疗前,且试验组的24 h内最长反流时间短于对照组,反流次数与反流时间>5 min次数均少于对照组,差异有统计学意义(P<0.05)。治疗后,两组患者的白介素-6(IL-6)、肿瘤坏死因子-α(TNF-α)水平均低于本组治疗前,胃泌素17(G-17)、降钙素基因相关肽(CGRP)水平均高于本组治疗前,且试验组的IL-6、TNF-α水平均低于对照组,G-17、CGRP水平均高于对照组,差异有统计学意义(P<0.05)。结论胆囊切除术后BRG使用康复新液、莫沙必利、胆石利通片联合治疗可有效调节炎症因子、胃肠激素水平,更好地改善患者胆汁反流情况,减轻临床症状,促进疾病恢复。

清凉汤含漱联合康复新液喷剂防治鼻咽癌放射性口腔炎的临床研究

目的:观察予以鼻咽癌(NPC)并发放射性口腔炎病人清凉汤含漱联合康复新液喷剂治疗的临床效果。方法:选取2022年6月—2023年8月医院收治的60例NPC并发放射性口腔炎病人(均初次接受根治性放射治疗、治疗前口腔黏膜完整)为研究对象,按照随机数字表法分为对照组、观察组,每组30例。对照组采取生理盐水含漱治疗;观察组给予清凉汤含漱联合康复新液喷剂治疗。比较两组病人放射性口腔炎发生时间、C反应蛋白(CRP)水平、中医证候积分、治疗效果及口腔炎分级。结果:观察组病人放射性口腔炎发生时间长于对照组(P<0.05);治疗后观察组病人CRP水平、中医证候积分均低于对照组;观察组病人总有效率为90.00%,高于对照组的63.33%(P<0.05);观察组病人口腔炎分级程度轻于对照组(P<0.05)。结论:予以NPC并发放射性口腔炎病人清凉汤含漱+康复新液喷剂治疗可延缓放射性口腔炎发生时间,改善病人临床症状,减轻机体炎症水平,获得良好防治效果。

康复新治疗牙周炎的机制研究

目的通过研究康复新对牙周组织相关细胞增殖的影响及康复新在细胞炎症反应模型中的抗炎作用,从促进牙周组织再生和抗炎2个方面探讨康复新治疗牙周炎的相关机制。方法(1)采用淋巴细胞增殖检测法(MTS法)检测不同浓度康复新对人牙周膜成纤维细胞(hPDLFs)、人牙龈上皮细胞(hGECs)、人单核巨噬细胞(THP-1)和小鼠胚胎成骨细胞(MC3T3-E1)活性的影响;(2)通过细菌脂多糖(LPS)刺激小鼠巨噬细胞RAW264.7建立细胞炎症模型,采用实时定量聚合酶链反应(qRT-PCR)法检测康复新对细胞白细胞介素-1β(IL-1β)、IL-10、一氧化氮合酶(NOS)和基质金属蛋白酶-13(MMP-13)mRNA表达水平的影响。结果(1)与对照组比较:0.1000、0.0500、0.0250、0.0125 mg/mL的康复新组在24 h和48 h时间点均可刺激hPDLFs、THP-1和MC3T3-E1增殖(P<0.01),且促增殖作用具有浓度依赖性;(2)在炎症细胞模型中,与对照组比较,0.0500 mg/mL和0.0125 mg/mL的康复新组IL-1β和IL-10 mRNA表达水平比较,差异均无统计学意义(P>0.05),NOS和MMP-13 mRNA表达水平降低,差异有统计学意义(P<0.05)。结论康复新在一定浓度范围内能促进hPDLFs、hGECs、THP-1和MC3T3-E1增殖;康复新可抑制LPS诱导的RAW264.7细胞炎症反应,其抗炎机制可能是降低NOS和MMP-13 mRNA表达水平。康复新可能是通过促进牙周组织再生和抗感染治疗牙周炎。

HPLC-DAD法测定康复新液中10种抑菌剂含量

目的:建立同时测定康复新液中10种抑菌剂的高效液相色谱分析方法。方法:采用Thermo Acclaim TM 120(4.6 mm×250 mm,5μm)色谱柱,流动相为0.1%冰醋酸-0.02 mol·L^(-1)乙酸铵溶液和甲醇,梯度洗脱,流速为1 mL·min^(-1),扫描波长为200~700 nm。结果:在相应的浓度范围内,10种抑菌剂质量浓度与峰面积的线性关系良好,r≥0.9998;精密度试验良好,RSD在0.33%~0.38%;重复性试验与稳定性试验的RSD<0.5%,加标回收范围为97.4%~101.1%,RSD在0.4%~1.8%之间。按拟定方法测试样品苯甲酸含量在0.15%~0.34%范围,山梨酸含量在0~0.48%范围,羟苯乙酯含量在0~0.02%范围、羟苯丙酯含量再0~0.02%范围。结论:该方法灵敏快速、专属性强、准确度高,可用于康复新液中抑菌剂的含量测定。

光活化消毒联合康复新液治疗中重度牙周炎的效果及对龈沟液炎性因子、生活质量的影响

目的探究光活化消毒联合康复新液治疗中重度牙周炎的效果及对龈沟液炎性因子、生活质量的影响。方法选取2021年1月至2023年6月郑州大学第一附属医院收治的104例中重度牙周炎患者作为研究对象,采用简单随机化法分为研究组和对照组各52例。两组患者均行龈下刮治和根面平整治疗,另给予对照组患者康复新液治疗,给予研究组患者光活化消毒联合康复新液治疗。比较两组患者的治疗效果,治疗前及治疗4周后的牙周指标(探诊深度、附着丧失度、菌斑及龈沟出血指数)、病原菌(牙龈卟啉单胞菌、福赛类杆菌、伴放线杆菌)、龈沟液炎性因子[白细胞介素-1β(IL-1β)、白细胞介素-6(IL-6)、肿瘤坏死因子-α(TNF-α)、人类软骨糖蛋白-39(HCgp-39)、C-反应蛋白(CRP)]、生活质量[口腔健康影响程度量表(OHIP-14)评价]和咀嚼功能,同时比较两组患者治疗期间的不良反应情况。结果治疗4周后,研究组患者的临床总有效率为92.31%,高于对照组的76.92%,差异有统计学意义(P<0.05);治疗4周后,研究组患者的探诊深度、附着丧失度、菌斑及龈沟出血指数分别为(3.16±0.35)mm、(3.01±0.23)mm、(0.85±0.21)分、(0.72±0.22)分,明显低于对照组的(3.94±0.37)mm、(3.85±0.26)mm、(1.37±0.28)分、(1.08±0.32)分,差异均有统计学意义(P<0.05);治疗4周后,研究组患者的龈沟液IL-1β、TNF-α、IL-6、CRP、HCgp-39水平分别为(4.95±0.95)pg/mL、(3.74±0.51)ng/L、(5.37±0.61)ng/L、(6.42±1.76)mg/L、(15.53±2.49)ng/mL,明显低于对照组的(6.34±1.38)pg/mL、(4.48±0.69)ng/L、(6.45±0.94)ng/L、(8.83±1.91)mg/L、(19.79±2.88)ng/mL,差异均有统计学意义(P<0.05);治疗4周后,研究组患者的牙龈卟啉单胞菌、福赛类杆菌、伴放线杆菌检出率分别为7%、4%、1%,明显低于对照组的16%、12%、8%,差异均有统计学意义(P<0.05);治疗4周后,研究组患者的OHIP-14评分为(9.41±2.17)分,明显低于对照组的(13.54±2.69)分,咀嚼效率为(77.26±8.15)%,明显高于对照组的(68.09±7.23)%,差异均有统计学意义(P<0.05);治疗期间研究组患者的不良反应总发生率为1.92%,略低于对照组的5.77%,但差异无统计学意义(P>0.05)。结论光活化消毒联合康复新液治疗中重度牙周炎疗效确切,可控制牙周炎症、病原菌发展,改善牙周状况,提高咀嚼能力,减少牙周炎对生活质量的影响,且安全性高。