Effects of Kangquan Recipe(康泉方) on Sex Steroids and Cell Proliferation in Rats with Benign Prostatic Hyperplasia

Objective:To investigate the effects of Kangquan Recipe(康泉方,KQR)on sex steroids and cell proliferation in an experimental benign prostatic hyperplasia(BPH)model in rats.Methods:Seventy-two SD rats were randomly divided into six groups:the normal group,the model group,the finasteride group,and the low-, middle-,and high-dose KQR groups,12 in each group.Except those in the normal group,the rats were injected with testosterone after castration for the establishment of BPH model and then given respectively with...

Effect of Kangquan Recipe(康泉方)on BAMBI Expression in Hypothalamic-Pituitary-Prostate in Rats with Benign Prostatic Hyperplasia

Objective:To investigate the effect of Kangquan Recipe(康泉方,KQR)on bone morphogenetic protein and activin membrane-bound inhibitor(BAMBI)expression and its mechanism in rats with benign prostatic hyperplasia(BPH).Methods:Forty-eight male Sprague-Dawley rats were divided into 6 groups using a random number table,with 8 in each group:the normal group(normal saline 10 m L/kg),the model group(normal saline 10 m L/kg),the finasteride group(0.5 mg/kg),the low-dose KQR group(3.5 g/kg),the middle-dose KQR group(7 g/kg),and the high-dose KQR group(14 g/kg).The 40 rats were subcutaneously injected with testosterone propionate after castration for 30 days to establish the BPH rat model except for those in the normal group.At the same time,the corresponding drugs were administered by gavage for 30 consecutive days.The effects of different doses of KQR on the protate wet weight,prostate volume and prostate index(PI)were observed.The changes in histopathology were monitored with hematoxylin-eosin staining.BAMBI protein and m RNA expression contents were determined by Western blot and quantitative real-time polymerase chain reaction,respectively.Results:All doses of KQR could decrease prostatic epithelial tissue proliferation.Compared to the model group,the high and middle-dose KQR significantly reduced prostate wet weight,prostate volume and PI;increased BAMBI protein expression in the hypothalamus,pituitary and prostate tissue;all doses of KQR up-regulated BAMBI m RNA expression in serum,prostatic fluid and prostate tissue(P<0.05 or P<0.01).Conclusions:KQR could inhibit the proliferation of rat prostatic tissue,promote BAMBI protein expression in the hypothalamic-pituitary-prostate of rats with BPH;and increase BAMBI m RNA expression in the blood,prostatic fluid and prostate tissue of rats with BPH,showing a dose-effect relationship.KQR can be used as a potential drug for the treatment of BPH.

Clinical Study on Kangquan Recipe (康泉方) for Benign Prostatic Hyperplasia Patients:A Randomized Controlled Trial

Objective：To observe the effectiveness and safety of Kangquan Recipe（康泉方,KQR） for benign prostatic hyperplasia（BPH） patients.Methods：One hundred and six BPH patients were randomly assigned to the treatment group（53 cases） and the control group（53 cases） according to a random number table.The treatment group was given KQR orally;the control group was given cemilton orally.After 24-week treatment,the clinical effect and safety were evaluated using the International Prostatic Symptom Score（l-PSS）,quality of life（QOL）,maximum flow rate（Qmax）,average flow rate（Qave）,residual urine volume（RUV）,total prostatic volume（TPV）,etc.Results：After treatment,the score of l-PSS was decreased from 16.9 ±5.6 to 12.5 ±4.6in the treatment group,significantly lower compared with the control group;the levels of Qmax and Qave were from 10.9 ±3.5 to 15.6 ±4.5 and 5.4 ±2.1 to 7.3 ±2.5（mL/s） in the treatment group,significantly higher compared with the control group;the levels of RUV and TPV were from 70.8 ±28.2 to 35.2 ±21.8 and37.2 ± 16.9 to 30.1 ± 10.8（mL） in the treatment group,significantly lower compared with the control group（all P〈0.05）.The incidence rate of adverse reaction was similar between the two groups（P〉0.05）.Conclusion：KQR is effective and safe for the treatment of BPH.