Objective To observe the effect of Xiaoer Feire Kechuan oral solution on the extrapulmonary injury induced by Mycoplasma pneumoniae in infant Wistar rats. Methods Infant Wistar rats were infected intranasally with M. ...Objective To observe the effect of Xiaoer Feire Kechuan oral solution on the extrapulmonary injury induced by Mycoplasma pneumoniae in infant Wistar rats. Methods Infant Wistar rats were infected intranasally with M. pneumoniae once a day for four days. In the treatment groups, Xiaoer Feire Kechuan oral solution was administered daily for four days beginning from the day of infection. On day 5, blood of the rats was collected, and blood routine and biochemistry indexes were measured. All rats were sacrificed, and the weight of brain, heart, liver, and kidney was measured to calculate the organ indexes. The GM1 and GALC-Ab content in brain tissue was determined by ELISA. Pathological changes in the brain, heart, liver, kidney, and cerebellum were observed by HE staining. Results Blood routine indexes fluctuated within the normal range in the infection control group and in three of the Xiaoer Feire Kechuan oral solution groups. The serum LDH, CK, and CRE in all three Xiaoer Feire Kechuan oral solution groups were distinctly lower than those in the infection control group (P < 0.01, P < 0.05). Rat brain index and GALC-Ab content in the brain tissue showed an increase in infection control group. In the Xiaoer Feire Kechuan oral solution groups, the GALC-Ab content in brain tissue was decreased significantly. The heart, liver, and kidney tissues showed mild pathological changes in the infection group, which were reversed by Xiaoer Feire Kechuan oral solution treatment. Conclusions The extrapulmonary injury induced by M. pneumoniae in infant Wistar rats was significantly inhibited by Xiaoer Feire Kechuan oral solution.展开更多
文摘Objective To observe the effect of Xiaoer Feire Kechuan oral solution on the extrapulmonary injury induced by Mycoplasma pneumoniae in infant Wistar rats. Methods Infant Wistar rats were infected intranasally with M. pneumoniae once a day for four days. In the treatment groups, Xiaoer Feire Kechuan oral solution was administered daily for four days beginning from the day of infection. On day 5, blood of the rats was collected, and blood routine and biochemistry indexes were measured. All rats were sacrificed, and the weight of brain, heart, liver, and kidney was measured to calculate the organ indexes. The GM1 and GALC-Ab content in brain tissue was determined by ELISA. Pathological changes in the brain, heart, liver, kidney, and cerebellum were observed by HE staining. Results Blood routine indexes fluctuated within the normal range in the infection control group and in three of the Xiaoer Feire Kechuan oral solution groups. The serum LDH, CK, and CRE in all three Xiaoer Feire Kechuan oral solution groups were distinctly lower than those in the infection control group (P < 0.01, P < 0.05). Rat brain index and GALC-Ab content in the brain tissue showed an increase in infection control group. In the Xiaoer Feire Kechuan oral solution groups, the GALC-Ab content in brain tissue was decreased significantly. The heart, liver, and kidney tissues showed mild pathological changes in the infection group, which were reversed by Xiaoer Feire Kechuan oral solution treatment. Conclusions The extrapulmonary injury induced by M. pneumoniae in infant Wistar rats was significantly inhibited by Xiaoer Feire Kechuan oral solution.
文摘目的探讨咳喘定穴位贴敷联合小柴胡汤合半夏厚朴汤治疗痰浊阻肺证慢性阻塞性肺疾病(Chronic obstructive pulmonary disease,COPD)患者的临床疗效。方法选取2020年9月—2022年10月期间海南医学院第二附属医院收治的痰浊阻肺证COPD患者144例,按随机数字表法分为西药组、中药组、中药联合贴敷组,每组各48例。西药组采取常规西医治疗,中药组在西药基础上采取小柴胡汤合半夏厚朴汤治疗,中药联合贴敷组在常规西医基础上采取咳喘定穴位贴敷及小柴胡汤合半夏厚朴汤。治疗4周后,观察比较3组患者临床疗效、不良反应发生率、疾病复发率及治疗前后中医证候积分、肺功能[第1秒用力呼气容积(Forced expiratory volume for 1 second,FEV_(1))、用力肺活量(Forced vital capacity,FVC)、FEV_(1)/FVC]、康复效果[慢性阻塞性肺疾病评估测试量表(Chronic obstructive Pulmonary Disease Assessment Test Scale,CAT)、呼吸困难程度评分(modified Medical Research Council,mMRC)、6 min步行距离试验(6 min walking distance test,6MWT)]。结果治疗后中药联合贴敷组总有效率93.75%(45/48)高于西药组77.08%(37/48)、中药组79.17%(38/48),差异有统计学意义(P<0.05)。治疗后3组患者中医证候痰多、喘息、咳嗽、倦怠乏力、腹胀纳呆、下肢水肿积分均较治疗前降低,差异有统计学意义(P<0.05),且中药联合贴敷组中医证候痰多、喘息、咳嗽、倦怠乏力、腹胀纳呆、下肢水肿积分均明显低于西药组、中药组,差异有统计学意义(P<0.05)。治疗后3组患者肺功能FEV_(1)、FVC、FEV_(1)/FVC指标均较治疗前升高,差异有统计学意义(P<0.05);且中药联合贴敷组肺功能FEV_(1)、FVC、FEV_(1)/FVC指标明显高于西药组、中药组,差异有统计学意义(P<0.05)。治疗后3组患者康复效果CAT、mMRC评分较治疗前降低,6MWT较治疗前延长,差异有统计学意义(P<0.05);且中药联合贴敷组康复效果CAT、mMRC评分均明显低于西药组、中药组,6MWT明显优于西药组、中药组,差异有统计学意义(P<0.05)。治疗期间,中药联合贴敷组不良反应发生率8.33%(4/48)与西药组6.25%(3/48)、中药组4.17%(2/48)比较,差异无统计学意义(P>0.05)。治疗6个月后中药联合贴敷组疾病复发率4.17%(2/48)低于西药组18.75%(9/48)、中药组16.67(8/48),差异有统计学意义(P<0.05)。结论联合采取咳喘定穴位贴敷及小柴胡汤合半夏厚朴汤治疗痰浊阻肺证COPD效果显著,可有效缓解患者临床症状,改善肺功能及机体运动耐力,利于提升疾病整体干预效果,降低疾病复发率,且具有安全性。
