EXPRESSION OF IL-2R ON PERIPHERAL BLOOD LYMPHOCYTES OF PATIENTS WITH COLORECTAL CANCER AND ITS CLINICAL SIGNIFICANCE

Objective: To study expression of membrane receptors of interleukin-2 (CD25) on the peripheral blood lymphocytes (PBL) of patients with colorectal cancer and its clinical significance. Methods: CD25 percentages (CD25%) in PBL of 105 colorectal cancer patients before operation and 100 normal individuals were examined by flow cytometer, and the results were clinically and pathologically analyzed. Results: The mean of CD25% in PBL of the normal individuals was 17.24±5.33, it was significantly lower (P<0.01) than that of the colon cancer patients (21.29±7.95) or rectal cancer patients (21.62±6.11). In contrast to the normal individuals, the means of CD25% in PBL in ulcer type (20.53±6.50) or protruded type (21.56±6.16) colorectal cancer patients were notably elevated (P<0.01). The significant difference (P<0.01) of means of CD25% in PBL was observed between the normal individuals and patients with less than 4 cm mass (22.10±5.43) or 4cm–8cm mass (20.90±6.96). The significant difference (P<0.05) of means of CD25% in PBL was also observed between the normal individuals and patients with greater than 8 cm mass (21.56±5.41). The mean of CD25% in PBL in patients with well differentiation colorectal cancer was 22.20±5.50, it was significantly higher than that in normal individuals (P<0.05). The means of CD25% in PBL in patients with middle or poor differentiation colorectal cancer were 21.30±6.89 and 22.15±5.71 respectively, they were obviously higher than that in normal individuals (P<0.01). The significant difference (P<0.01) of means of CD25% in PBL was present between the colorectal cancer patients without metastatic lymph nodes (22.06±6.90) and normal individuals. The significant difference (P<0.05) of means of CD25% in PBL was present between the colorectal cancer patients with metastatic lymph nodes (20.73±6.40) and normal individuals. The means of CD25% in PBL in colorectal cancer patients in various clinic stages were significantly higher than that in the health subjects (P<0.01). The significant difference (P<0.01) of means of CD25% in PBL was present between the patients whose ages were equal to or less than 60 (21.00±5.76) and normal individuals in the same age group. The significant difference (P<0.05) of means of CD25% in PBL was also present between the patients whose ages were greater than 60 (22.54±7.75) and normal individuals in the same age group. The significant difference (P<0.01) of means of CD25% in PBL was present between the male patients (22.55±7.05) and normal men. The significant difference (P<0.05) of means of CD25% in PBL was also present between the female patients (20.09±5.48) and normal women. Conclusion: The mean of CD25% in PBL of colorectal cancer patients was significantly higher than that in health subjects. Abnormally elevated CD25% were correlative with site of tumor growth, macropathology type of tumor, the degree of tumor differentiation, clinical stage and patient’s age and sex. It may be helpful to detect CD25% in PBL of colorectal cancer patients before operation for diagnosis, immune treatment and judging prognosis.

输血对老年股骨粗隆间骨折隐性失血的影响

目的探讨输血治疗是否是老年股骨粗隆间骨折围手术期隐性失血量的影响因素,并为是否需要输血治疗提供参考。方法回顾性分析2009年3月至2012年10月期间接受股骨近端防旋髓内钉(proximal femoral nail anti-rotation,PFNA)内固定手术的176例老年股骨粗隆间骨折患者的临床相关资料,依据是否接受输血治疗将患者分为输血组(56例)及未输血组(120例),对两组患者术前、术后血常规指标以及根据Gross线性方程计算得到的总失血量、隐性失血量进行分析,比较输血治疗对隐性失血量的影响。结果输血组平均隐性失血量为353.0 mL,占平均总失血量比例为83.1%,未输血组患者平均隐性失血量为427.0 mL,占总失血量比例为84.6%。两组平均隐性失血量相比,有统计学意义(P<0.05),平均隐性失血量占总失血量比例,两组比较无统计学意义(P>0.05)。结论输血治疗能减少围手术期隐性失血量,但并不能降低老年粗隆间骨折的隐性失血量占总失血量的比例,术后应根据红细胞压积(hematocrit,Hct)及血红蛋白量(hemoglobin,Hb)的变化,合理选择输血治疗,减少并发症的产生。

Comparison of blood rheological models in patient specific cardiovascular system simulations

Newtonian, Quemada and Casson blood viscosity models are implemented in order to simulate the rheological behavior of blood under pulsating flow conditions in a patient specific iliac bifurcation. The influence of the applied blood constitutive equations is monitored via the wall shear stress （WSS） distribution, magnitude and oscillations, non-Newtonian importance factors, and viscosity values according to the shear rate. The distribution of WSS on the vascular wall follows a pattern which is independent of the theological model chosen. On the other hand, the WSS magnitude and oscillations are directly related to the blood constitutive equations applied and the shear rate. It is concluded that the Newtonian approximation is satisfactory only in high shear and flow rates. Moreover, the Newtonian model seems to overestimate the possibility for the formation of atherosclerotic lesions or aneurysms at sites of the vascular wall where the WSS are oscillating.

基于共词分析法分析我国新生儿输血的研究现状

目的通过共词分析法对我国新生儿输血领域的相关文献进行分析,旨在分析我国新生儿输血的研究现状。方法本研究以"新生儿""输血"为关键词,在中国知网(CNKI)数据库检索文献,并限定文献发表时间为2000年1月1日至2016年11月30日,共计获得符合本研究纳入标准与排除标准的文献259篇为研究对象。将获取的259篇文献,采用Microsoft Office Excel 2016对各关键词及其词频进行统计和分析,提取合适的高频关键词,并对其建立共现矩阵。利用Ucinet 6.3,对高频关键词共现矩阵绘制可视化网络,对高频关键词的关联性进行分析。结果(1)符合本研究纳入标准与排除标准的文献共计259篇,提取词频≥5次的高频关键词37个,包括"新生儿""换血""新生儿溶血病""输血""新生儿高胆红素血症""新生儿贫血"等。(2)本研究高频关键词共现关系图分析结果显示,在我国新生儿输血研究领域中,以"新生儿""输血"为核心,"新生儿溶血病"为主要研究热点,其余大部分研究集中于"换血""新生儿高胆红素血症"等方面,关系最为密切的高频关键词是"新生儿"与"换血","换血"与"新生儿高胆红素血症"。结论目前我国新生儿输血领域的研究主要集中于新生儿溶血病、新生儿高胆红素血症、新生儿贫血等疾病,以及换血疗法方面。在新生儿输血护理、早产儿输血等方面的研究亦有较多涉猎,但是在输血安全、成分输血、输血指征等方面的研究仍较少,如何有效、合理、安全、规范的进行新生儿输血治疗,仍有待进一步研究。