Experimental Study of the Anti-human Papilloma Virus Mechanisms by a Traditional Chinese Medicine “Keyouling”

To investigate the anti-human papilloma virus (HPV) effect of the external liniment of traditional Chinese medicine “Keyouling”, the efficacy of this drug acting upon the proliferation and growth of the normal epidermis cells of rabbits′ penis prepuce in different concentrations was observed. An experimental model of pathology of histocytes infected with HPV in vitro was established by using the human HPV infected prepuce epidermis cells as virus carrier to infect the sub-cultured epidermis cells of rabbits′ penis prepuce. The direct killing effect and the blocking effect of infections of epidermis cells in rabbits′ penis prepuce induced by HPV with different concentrations of “Keyouling” were determined by 3 kinds of culture methods: (1) the infected epidermis cells of rabbits′ penis prepuce were challenged by co-culture with HPV suspension and with culture medium containing drug; (2) the co-culture with epidermis cells of rabbits′ penis prepuce and HPV suspension was challenged with drug; (3) co-culture with culture medium containing drug and epidermis cells of rabbits′ penis prepuce was challenged with HPV suspension. It was found that “Keyouling” had little effect on the proliferation and growth of the normal epidermis cells of rabbits′ penis prepuce, but it had direct killing effect on HPV, and blocked effectively the infection of the epidermis cells of rabbits′ penis prepuce by HPV. It concludes that “Keyouling” has significant killing effect to HPV, but not normal histocytes in vitro , indicating that it is safe for clinical use. Its blocking effect on the HPV infection of rabbits′ penis prepuce epidermis cells suggested “Keyouling” may be clinically used to prevent the development of tumors or to inhibit the further progression or metastasis of tumors.

中药克疣灵局部应用联合微波治疗女性尖锐湿疣的疗效观察

目的:观察中药克疣灵局部应用联合微波治疗女性尖锐湿疣的疗效。方法:选取2009年1月-2011年1月我院收治的122例女性尖锐湿疣患者为研究对象,按照就诊顺序随机均分为两组,观察组61例患者给予中药克疣灵局部应用联合微波治疗,对照组61例患者给予单纯微波治疗,比较两组患者治疗后1周和治疗后1月的临床效果。结果:随治疗时间延续,观察组和对照组治疗后1月时总显效率均高于治疗后1周时,但比较差异均无统计学意义(P>0.05);观察组患者治疗1周时总显效率为81.97%,高于对照组57.38%,治疗1月时总显效率为95.08%,高于对照组65.57%,比较差异均有统计学意义(P<0.05)。结论:克疣灵联合微波治疗女性尖锐湿疣,疗法简便易行,疗效显著优于单用微波治疗,值得临床推广。

中药克疣灵治疗尖锐湿疣调节细胞免疫功能的实验和临床研究

目的 :探讨中药克疣灵口服液治疗尖锐湿疣 (CA)调节细胞免疫功能的作用机制。 方法 :采用ELISA双抗体夹心法测定经克疣灵作用后的造模大鼠、CA患者外周血清及疣体组织白细胞介素 18(IL 18)、肿瘤坏死因子 α(TNF α)水平 ,采用3 H 甲基胸腺嘧啶脱氧核苷 (3 H TdR)释放法测定经克疣灵作用后的造模大鼠脾脏及CA患者外周血自然杀伤 (NK)细胞活性。 结果 :动物实验克疣灵高剂量组TNF α、IL 18水平、NK细胞活性与模型组、低剂量组比较差异有显著性 (P <0 .0 5 ) ;治疗组治疗后血清、疣体组织TNF α、IL 18水平、NK细胞活性与对照组比较 ,差异均有显著性 (P <0 .0 1及P <0 .0 5 )。 结论 :克疣灵口服液具有显著的正向调节作用 ,能显著改善造模动物细胞免疫缺陷 。

“克疣灵”对尖锐湿疣细胞增殖影响作用的实验研究

目的 :探讨中药“克疣灵”治疗CA的作用机理。方法 :采用细胞原代传代培养技术、MTT比色法测定不同浓度“克疣灵”对兔包皮表皮细胞、CA疣体组织细胞增殖生长的影响 ,采用FQ -PCR定量检测不同浓度“克疣灵”对HPV6 11DNA表达水平及对CA患者阴茎包皮下不同区域HPV6 11DNA表达水平的影响作用。结果 :“克疣灵”对兔包皮表皮细胞增殖无影响 ,而CA疣体组织细胞增殖生长具有显著的抑制作用。结论 :能显著抑制CA疣体组织细胞的增殖生长 ,而对机体正常表皮细胞无损害作用。提示“克疣灵”临床应用安全。

“克疣灵”对人包皮表皮细胞和尖锐湿疣疣体组织细胞增殖影响的实验研究

目的 :探讨中药“克疣灵”治疗尖锐湿疣 (CA)的作用机制。 方法 :采用细胞原代与传代培养技术、MTT比色法测定人包皮表皮细胞、CA细胞的增殖程度 ,不同浓度“克疣灵”对人包皮表皮细胞、CA细胞增殖的影响。 结果 :吸光度值 (A值 )与人包皮表皮细胞、CA细胞数量呈正相关 (人包皮细胞组r=0 .985 0 ,CA细胞组r =0 .9892 ,P均 <0 .0 0 1) ,“克疣灵”对人包皮表皮细胞增殖生长无影响 ,而对CA细胞增殖具有显著的抑制作用 ,“克疣灵”浓度与CA细胞增殖率呈显著负相关 (r =- 0 .4 12 4 ,P <0 .0 1)。 结论 :“克疣灵”能显著抑制CA细胞的增殖生长 ,而不影响机体正常表皮细胞生长。提示“克疣灵”可能具有抗人乳头瘤病毒作用。