Mapping theme trends and knowledge structures for human neural stem cells:a quantitative and co-word biclustering analysis for the 2013-2018 period

Neural stem cells,which are capable of multi-potential differentiation and self-renewal,have recently been shown to have clinical potential for repairing central nervous system tissue damage.However,the theme trends and knowledge structures for human neural stem cells have not yet been studied bibliometrically.In this study,we retrieved 2742 articles from the PubMed database from 2013 to 2018 using "Neural Stem Cells" as the retrieval word.Co-word analysis was conducted to statistically quantify the characteristics and popular themes of human neural stem cell-related studies.Bibliographic data matrices were generated with the Bibliographic Item Co-Occurrence Matrix Builder.We identified 78 high-frequency Medical Subject Heading(MeSH)terms.A visual matrix was built with the repeated bisection method in gCLUTO software.A social network analysis network was generated with Ucinet 6.0 software and GraphPad Prism 5 software.The analyses demonstrated that in the 6-year period,hot topics were clustered into five categories.As suggested by the constructed strategic diagram,studies related to cytology and physiology were well-developed,whereas those related to neural stem cell applications,tissue engineering,metabolism and cell signaling,and neural stem cell pathology and virology remained immature.Neural stem cell therapy for stroke and Parkinson’s disease,the genetics of microRNAs and brain neoplasms,as well as neuroprotective agents,Zika virus,Notch receptor,neural crest and embryonic stem cells were identified as emerging hot spots.These undeveloped themes and popular topics are potential points of focus for new studies on human neural stem cells.

Sickle Cell Gene Transmission: Do Public Servants in Tamale, Ghana Have the Right Knowledge and Attitude to Curb It?

Introduction: Sickle cell disease (SCD) is exerting enormous public health and economic burden on many countries. To reduce these effects, the World Health Organization is urging countries, especially those in Africa, to increase public awareness of this disease. This study accessed whether public servants in Tamale, Ghana possess adequate knowledge and positive attitude towards SCD. Method: A total of 320 public servants within the Tamale Metropolis were invited to complete a questionnaire in a cross-sectional study. Data were analyzed using GraphPad 5.01. Association between different variables was tested. Results: The media was the main source of information for a majority of the 94.2% of respondents who had ever heard of SCD. Knowledge of respondents on transmission and testing of SCD was above 70% but they recorded less than 40% in SCD’s incurability, prevalence, effect of SCD on life span and pregnancy. The mean knowledge score of respondents was (11.1 ± 5.11)/22 or 50.7%. Only about a quarter knew their sickling status or that of their spouses before marriage or first child. Although 78.0% of respondents would call off marriages if they became aware of genetic incompatibility, the overall mean attitude, (2.857 ± 1.97)/ 7.0% or 40.8% was poor. There was a positive and significant correlation between the knowledge of respondents and their attitude towards SCD (r = 0.494, p < 0.0001) hence respondents working in the health sector and those with tertiary level education had better attitudes towards SCD. Conclusion: Public servants in Ghana have moderate knowledge of SCD but their attitude towards the disease was poor. Public servants least considered genetic incompatibility before entering conjugal relationships. This poor attitude means the campaign for the reduction of sickle cell gene transmission must be intensified. Governments need to increase their investment on educational campaigns aimed at changing attitude of persons through provision of more knowledge about SCD.

Cell Biology and Ethics: Knowledge, Attitudes and Practices of Anatomists in India

Most scientific discoveries and advancements have occurred in the world in the last century. Both developed and developing countries are the beneficiaries of the new scientific knowledge and its application. Advanced knowledge and the ethical issues are inseparable from research, treatment, prophylaxis and prevention involving human diseases. It is necessary to make well informed decisions about acceptance of advancements that have taken place elsewhere and also prepare systems for our research and medical education. Bioethics therefore needs to be grounded in the local socio cultural context and health care systems. Students make first contact with human biological tissue in the Anatomy department. The seeds of ethical practices and professionalism are sown here. Therefore appropriate knowledge and attitudes of anatomists and cell biologists towards human tissue is important for regular practice of medicine. A questionnaire based study of knowledge and attitudes of 300 anatomy faculty related to use of human biological materials in research and education was conducted. The items included in the questionnaire were to judge the awareness about the same i.e., laboratory handling of human material and research related to corpses, organ & whole body donation along with their use in clinical learning and research in various medical specialties like, orthopedics, surgery, ENT, anesthesia etc. for clinicians, physicians and surgeons as well as undergraduate, post graduate and allied workforce teaching. The data collected from the respondents in the form of positive and negative responses was analyzed. The study identifies the knowledge gaps and the perceptions of faculty regarding the scope and utility of understanding the role of human biological tissues in curriculum and research and transmission of message to the next generation of learners.

基于关键词共现分析的国内外未来产业发展重点研究

为梳理筛选未来产业主要领域,为后续研究提供支撑,以CiteSpace等软件作为工具,通过共词关系获得国内外未来产业相关文献图谱,从宏观上得到未来产业概况,结合国外主要发达国家战略政策以及全国31个省(自治区、直辖市)规划、未来产业专项规划等政府文件,梳理未来产业国内外发展的重点和差异,为进一步筛选和研究提供基础。经研究发现,国内外对未来产业的重点关注领域大体相同,主要集中在智能、低碳和健康三大领域,人工智能是重点关注领域;未来产业呈现多学科、多技术的高度交叉和深度融合趋势,技术创新是核心驱动力,生产制造是关键,而资金投入、人才培养和平台建设则是未来产业发展的三大着力点。

基于CiteSpace的河洛文化研究态势分析

河洛文化是中华民族的根文化和中国传统文化的源头。研究解读河洛文化,对于建设新时代中华民族精神家园,实现中华民族伟大复兴意义重大。基于SiteSpace科学文献计量知识图谱研究方法,以1984~2023年间中国知网收录的757篇河洛文化论文为研究对象,从“发文时间分布”“关键词共现”“关键词聚类”“关键词突显”4个方面,探究其发展历程与现状。目前,河洛文化研究虽然取得了一系列丰硕成果,但仍有需要深入拓展的学术领域。

基于Web of Science近十年干细胞治疗心肌梗死的可视化分析

背景:心肌梗死的致死率居高不下,是因为包括药物和手术在内的传统治疗方法无法从根本上修复受损的心肌组织,干细胞由于其自我更新和多向分化潜能为解决这一难题提供了可能。目的:运用文献计量学分析归纳干细胞治疗心肌梗死领域近十年的研究进展。方法:检索Web of Science数据库中2012-01-01/2022-12-01发表在SCI-E和SSCI中干细胞和心肌梗死的相关文献,利用EXCEL、CiteSpace和VOSviewer软件对发文量、作者、机构、期刊、国家、关键词等数据进行统计及可视化分析。结果与结论:核心文献共计3210篇,年发文总量逐年上升;hausenloy,derek j.是发文量最多的作者,中国是发文量最多的国家,第四军医大学是发文量最多的机构,该领域的研究热点正在从细胞实验和动物实验向临床试验方向转变。近十年来,该领域的研究热度较高,仍有较大的发展前景,应促进国际上、机构间的交流学习,进一步挖掘干细胞在心肌梗死治疗领域的作用。

基于CiteSpace的蒙古族服饰研究进展的可视化分析

以中国知网(CNKI)数据库和Web of Science(WOS)为核心合集数据库,从中搜索近40年与蒙古族服饰研究密切相关的文献,并以之为研究对象,运用文献计量学软件CiteSpace对该研究主题的发文量、来源期刊、核心作者、研究机构、关键词共现与聚类、相关研究热点、突现词等进行可视化呈现和分析,采用关键词聚类分析法进行聚类分析,得到15个主题4大类别的聚类,生成关键词聚类、关键词突现等图谱。结果表明:蒙古族服饰相关研究自1983年开始,发文量总体呈波动上升态势;研究学者及学术机构具有明显的地域局限性;研究热点包括蒙古族历史发展与文化交融、服饰基本属性、文化传承发展和现代创新设计4个方面。多元服饰文化与现代科学技术相互交融的创新设计热潮是蒙古族服饰研究的未来发展趋势。

中国学者在世界顶级期刊的发文分析--基于2000-2015年Cell、Nature和Science的载文统计分析

[目的/意义]Cell、Nature和Science是目前公认的世界顶级期刊,在Cell、Nature和Science上发文,一定程度上意味着对学者科研成果的高度肯定。[方法 /过程]利用文献计量方法对Web of Science数据库中收录的Nature、Science和Cell杂志2000-2015年发表的中国学者的论文进行研究,从发文量、核心作者、发文机构、文献被引情况以及研究主题进行了全面分析。[结果 /结论]随着时间段的推移,中国学者在Cell、Nature和Science上发文的作者从徐星教授为代表的作者群演变为以王俊为代表的作者群;研究热点逐渐从古生物学研究转变为生命科学研究,生命科学研究已成为目前的研究热点;研究机构从中科院古脊椎动物与古人类研究所为代表的机构群逐渐转变为以深圳华大基因研究院为代表的机构群。

基于CiteSpace的空间句法研究热点 与趋势可视化分析

空间句法作为一种分析和解释城市与建筑空间布局及其对人类行为和社会动态的影响的理论和方法工具,已经引起了国际和国内学术界的广泛关注。研究基于中文核心(CNKI)和英文核心(WoS)数据库,覆盖了2003-2023年的相关核心文献。运用CiteSpace软件进行了关键词分析和知识图谱的绘制。结果表明:①空间句法作为研究领域在国际和国内层面均获得了持续增长的关注;②中英文数据库在空间句法研究上表现出既有共同点又有各自独特的发展路径和研究重点;③国内外在空间句法研究的未来发展方向上存在显著差异,这为双方提供了互相借鉴和学习,共同推动该领域的创新发展的机会。未来研究方向包括:①继续深化中英文数据库的比较研究;②加强国际与国内的研究合作,共享数据和成果,同时尊重各自的研究特色和研究方法。研究成果可为空间句法研究的深入扩展提供参考。

基于COOC和VOSviewer的肝移植病人门静脉血栓研究知识图谱分析

目的:了解肝移植病人门静脉血栓(PVT)研究热点和发展趋势。方法:检索Web of Science(核心合集)数据库中发表于2002年1月1日—2022年12月31日的与肝移植病人PVT相关的文献,使用COOC 13.5和VOSviewer 1.6.13对纳入文献的国家、机构、关键词等内容进行可视化分析。结果:共纳入1157篇文献,随着时间推移,文献数量总体呈上升趋势。美国是发文量最高的国家,四川大学、香港大学、东京大学、Beaujon医院较早进行肝移植病人PVT相关研究。关键词共现聚类分析结果显示,肝移植病人PVT相关研究主要为小儿肝移植、肝细胞癌伴门静脉癌栓治疗、介入治疗、抗凝治疗。肥胖、糖尿病和非酒精性脂肪肝是肝移植病人PVT研究趋势。结论:肝移植病人PVT研究逐渐增多,小儿肝移植、肝细胞癌伴门静脉癌栓治疗、介入治疗、抗凝治疗是其研究热点,代谢性疾病是其未来研究趋势。

非编码RNA与间充质干细胞研究的相关计量学分析

背景:非编码RNA作为一类不编码蛋白质的RNA分子,在细胞调控中发挥关键作用。同时,间充质干细胞具有多向分化潜能,对组织修复和再生至关重要。近年来,研究者们对非编码RNA在调控间充质干细胞功能中的作用展开了深入探讨。目的:通过文献计量学分析方法系统地了解非编码RNA与间充质干细胞相关研究的发展趋势与关键领域。方法:从Web of Science核心合集1990年至今的科学引文索引扩展获取非编码RNA与间充质干细胞相关研究的文献数据,运用VOSviewer和Cite Space计量学软件对年份、国家或地区、研究机构、被引文献和关键词进行发文量、聚类、被引频次、突增性和中介中心性进行分析,揭示该研究领域的知识基础及前沿热点。结果与结论:(1)最终纳入5348篇文献,发表在1997-2021年,相关文献呈现明显的增长趋势,尽管在2022年略有减少,其发文量仍保持较高水平,中国在该领域的研究中占据主导地位,其中上海交通大学为最活跃的机构。(2)对被引文献分析发现,高突增性、高中介中心性、高被引的文献主要与微小RNA、细胞外囊泡和骨代谢等内容相关,这些文献构成了非编码RNA与间充质干细胞研究领域的重要知识基础。(3)对关键词进行突增性分析发现,至今保持高突增性的关键词包括外泌体、环状RNA、细胞外囊泡和损伤。(4)对关键词进行聚类演变分析,发文量仍保持增长趋势的关键词聚类包括:肿瘤微环境、骨关节炎、氧化应激和细胞外囊泡,这些关键词反映了目前以及未来该领域的研究热点。(5)文章结果不仅展示了非编码RNA与间充质干细胞研究领域的研究态势,更重要的是有望为研究人员提供潜在的方向和启示。

基于CiteSpace的尾矿农业化利用研究现状及热点分析

以中国学术期刊全文数据库和Web of Science核心合集数据库中收录的尾矿农业化利用研究成果为基础,利用CiteSpace软件,采用知识图谱和文献计量学方法对1935-2023年期间开展的尾矿农业化利用研究成果进行了分析,结果表明:尾矿农业化利用研究在1935-1992年、1993-2002年、2003-2012年和2013年至今4个时期,形成了尾矿与土壤肥力、尾矿与土壤改良和尾矿与重金属等三大研究领域,共10个主要研究方向;2013年以来,尾矿改性对土壤物理结构的影响研究和尾矿基质土在可持续农业中的应用研究是主要研究热点;未来以土壤肥力提升与有毒元素降低协同增效、高效改良剂和生物炭基改良剂制备与应用、土壤碳氮循环与温室气体排放等将是尾矿农业化利用研究热点。

基于知识蒸馏的宫颈细胞图像分类研究

宫颈癌筛查对宫颈癌预防和早期宫颈癌诊断具有重要意义。针对现有宫颈细胞图像分类模型泛化能力不足、参数量大、对硬件要求高且难以部署终端等问题,提出一种基于知识蒸馏的宫颈细胞图像分类方法。使用残差网络为骨干网络,以ResNet18为基础学生网络,引入知识蒸馏机制使用ResNet34作为教师网络进行指导学习。采用迁移学习方法提高教师模型基准精度;将教师网络概率预测知识通过知识蒸馏传递给学生网络进行学习,以提升学生模型分类准确率。实验结果表明:知识蒸馏优化后的学生网络ResNet18精度高达95.59%,相比未优化前精度91.13%提升了4.46个百分点。蒸馏优化后的模型参数量小、精度高,网络的整体性能优秀,为建立临床轻量级宫颈细胞图像分类模型研究提供了参考。

Tamoxifen induced multinucleated cells (symplasts) and distortion of seminiferous tubules in rat testis

Aim: To evaluate the effect of tamoxifen citrate on male reproductive system of rat. Methods: Groups of male rats were gavaged with tamoxifen at doses of 200 mg·kg-1·d-1, 400 mg·kg-1·d-1 or 800 mg·kg-1·d-1 in 0.1 mL olive oil for 10 consecutive days. Controls were treated with 0.1 mL olive oil. Rats were anesthetized and killed on d 3, d 15 or d 35 after the last dose. Testes were collected, processed for paraffin embedding, sectioned at 5 μm thickness, stained with H&E and analyzed microscopically. Results: There was a dose-dependent increase in the occurrence of seminiferous tubular distortion with germinal cell sloughing. The highest dose increased the number of multinucleated giant cells on d 3 and d 15. Conclusion: Tamoxifen citrate induces multinucleated giant cells and germinal epithelial sloughing in a dose-dependent manner and these changes are detrimental to male fertility.

A knowledge discovery method based on analysis of multiple co-occurrence relationships in collections of journal papers

Purpose: This paper explores a method of knowledge discovery by visualizing and analyzing co-occurrence relations among three or more entities in collections of journal articles.Design/methodology/approach: A variety of methods such as the model construction,system analysis and experiments are used. The author has improved Morris' crossmapping technique and developed a technique for directly describing,visualizing and analyzing co-occurrence relations among three or more entities in collections of journal articles.Findings: The visualization tools and the knowledge discovery method can efficiently reveal the multiple co-occurrence relations among three entities in collections of journal papers. It can reveal more and in-depth information than analyzing co-occurrence relations between two entities. Therefore,this method can be used for mapping knowledge domain that is manifested in association with the entities from multi-dimensional perspectives and in an all-round way.Research limitations: The technique could only be used to analyze co-occurrence relations of less than three entities at present.Practical implications: This research has expanded the study scope of co-occurrence analysis.The research result has provided a theoretical support for co-occurrence analysis.Originality/value: There has not been a systematic study on co-occurrence relations among multiple entities in collections of journal articles. This research defines multiple co-occurrence and the research scope,develops the visualization analysis tool and designs the analysis model of the knowledge discovery method.

Review： Perspectives on the metallic interconnects for solid oxide fuel cells

The various stages and progress in the development of interconnect materials for solid oxide fuel cells (SOFCs )over the last two decades are reviewed. The criteria for the application of materials as interconnects are highlighted. Interconnects based on lanthanum chromite ceramics demonstrate many inherent drawbacks and therefore are only useful for SOFCs operating around 1000℃. The advance in the research of anode-supported flat SOFCs facilitates the replacement of ceramic interconnects with metallic ones due to their significantly lowered working temperature. Besides, interconnects made of metals or alloys offer many advantages as compared to their ceramic counterpart. The oxidation response and thermal expansion behaviors of various prospective metallic interconnects are examined and evaluated. The minimization of contact resistance to achieve desired and reliable stack performance during their projected lifetime still remains a highly challenging issue with metallic interconnects. Inexpensive coating materials and techniques may play a key role in promoting the commercialization of SOFC stack whose interconnects are constructed of some current commercially available alloys. Alternatively, development of new metallic materials that are capable of forming stable oxide scales with sluggish growth rate and sufficient electrical conductivity is called for.

The Property of Nao Yi-an Granule (脑溢安颗粒) in Increasing Nestin Immunoreactive Neural Stem Cells in Rat Brains with Intracerebral Hemorrhage

Objective: To explore changes of neural stem cells (NSCs) following intracerebral hemorrhage (ICH) and the effect of Nao Yi-an granule (脑溢安颗粒, NYA) on them, and furthermore, to explore NYA's therapeutic mechanism Methods: Sixty rats were randomly divided into three groups, the sham operation control group, the ICH group and the NYA-treated group. Except those in the sham operation control group, all other rats were replicated to ICH models by stereotactical injection of collagenase type Ⅶ into their globus pallidus. After modeling, the rats in the NYA-treated group were administered with NYA at the dosage of 4. 92 g·kg-1·d-1 by gastrogavage, while those in the other two groups, 4 ml distilled water were given instead. The behavioral test was used to evaluate neurological deficit and immunohistochemical method was used to detect Nestin expression, the special marker for neural stem cells. Results: The ICH animals showed notable placing deficit in forlimbs from day 1 to day 28 after modeling. The deficit in the NYA-treated group was similar to that of the ICH group, but the former got recovered better than the latter from day 21 to 28, with significant difference shown ( P<0.05). Nestin-positive cells (N+ cells) could be seen around the hemotoma from day 2 on in the ICH group, the number of which gradually increased from day 4 to 7 ( P< 0.01) and reached the peak on day 14 (P<0.05), but reduced significantly on day 28 (P<0.01). Compared with that of the ICH group at the same time points , there was no significant difference in number of N+ cells in the NYA group on day 2 and 4, but it reached the peak earlier on day 7(P<0.01),with the level significantly higher than that in the ICH group( P<0. 01), and this high level lasted to day 14. Conclusion: NYA could promote the recovery of behavioral deficit in collagenase type Ⅶ-induced ICH rats, and increase the number of nestin-immunoreactive NSCs, suggesting that its effect on NSCs may be one of its pharmaceutical mechanisms in treating ICH.

Extracting Knowledge from On-Line Forums for Non-Obstructive Psychological Counseling Q&A System

Psychological counseling Q&A system is enjoying a remarkable and increasing popularity in recent years. Knowledge base is the important component for such kind of systems, but it is difficult and time-consuming to construct the knowledge base manually. Fortunately, there emerges large number of Q&A pairs in many psychological counseling websites, which can provide good source enriching the knowledge base. This paper presents the method of knowledge extraction from psychological consulting Q&A pairs of on-line psychological counseling websites, which include keywords, semantic extension and word sequence. P-XML, which is the knowledge template based on XML, is also designed to store the knowledge. The extracted knowledge has been successfully used in our non-obstructive psychologycal counseling system, called P.A.L., and the experimental results also demonstrated the feasibility and effectiveness of our approach.