Effects of tree size and organ age on variations in carbon,nitrogen,and phosphorus stoichiometry in Pinus koraiensis

Carbon(C),nitrogen(N),and phosphorus(P)are of fundamental importance for growth and nutrient dynamics within plant organs and deserve more attention at regional to global scales.However,our knowledge of how these nutrients vary with tree size,organ age,or root order at the individual level remains limited.We determined C,N,and P contents and their stoichiometric ratios(i.e.,nutrient traits)in needles,branches,and fine roots at different organ ages(0-3-year-old needles and branches)and root orders(1st-4th order roots)from 64 Pinus koraiensis of varying size(Diameter at breast height ranged from 0.3 to 100 cm)in northeast China.Soil factors were also measured.The results show that nutrient traits were regulated by tree size,organ age,or root order rather than soil factors.At a whole-plant level,nutrient traits decreased in needles and fine roots but increased in branches with tree size.At the organ level,age or root order had a negative effect on C,N,and P and a positive effect on stoichiometric ratios.Our results demonstrate that nutrient variations are closely related to organ-specific functions and ecophysiological processes at an individual level.It is suggested that the nutrient acquisition strategy by younger trees and organ fractions with higher nutrient content is for survival.Conversely,nutrient storage strategy in older trees and organ fractions are mainly for steady growth.Our results clarified the nutrient utilization strategies during tree and organ ontogeny and suggest that tree size and organ age or root order should be simultaneously considered to understand the complexities of nutrient variations.

Growth simulation and yield prediction for perennial jujube fruit tree by integrating age into the WOFOST model

Mathematical models have been widely employed for the simulation of growth dynamics of annual crops,thereby performing yield prediction,but not for fruit tree species such as jujube tree(Zizyphus jujuba).The objectives of this study were to investigate the potential use of a modified WOFOST model for predicting jujube yield by introducing tree age as a key parameter.The model was established using data collected from dedicated field experiments performed in 2016-2018.Simulated growth dynamics of dry weights of leaves,stems,fruits,total biomass and leaf area index(LAI) agreed well with measured values,showing root mean square error(RMSE) values of 0.143,0.333,0.366,0.624 t ha^-1 and 0.19,and R2 values of 0.947,0.976,0.985,0.986 and 0.95,respectively.Simulated phenological development stages for emergence,anthesis and maturity were 2,3 and 3 days earlier than the observed values,respectively.In addition,in order to predict the yields of trees with different ages,the weight of new organs(initial buds and roots) in each growing season was introduced as the initial total dry weight(TDWI),which was calculated as averaged,fitted and optimized values of trees with the same age.The results showed the evolution of the simulated LAI and yields profiled in response to the changes in TDWI.The modelling performance was significantly improved when it considered TDWI integrated with tree age,showing good global(R2≥0.856,RMSE≤0.68 t ha^-1) and local accuracies(mean R2≥0.43,RMSE≤0.70 t ha^-1).Furthermore,the optimized TDWI exhibited the highest precision,with globally validated R2 of 0.891 and RMSE of 0.591 t ha^-1,and local mean R2 of 0.57 and RMSE of 0.66 t ha^-1,respectively.The proposed model was not only verified with the confidence to accurately predict yields of jujube,but it can also provide a fundamental strategy for simulating the growth of other fruit trees.

N,P,and K characteristics of different age groups of temperate coniferous tree species in northwestern China

This study chose dominant tree species including Picea crassifolia,Pinus armandii and Pinus tabuliformis which are distributed in Qilian Mountains,Xiaolongshan Mountains,and Bailongjiang River.According to the different tree species,ages and components,we sampled leaves,branches,stems,and roots,and measured the contents of Nitrogen,Phosphorus,Potassium,along with soil fertility.The changes of N,P,and K contents in the different tree species were studied,and the relationship between nutrient content and environmental factors was analyzed.The results indicated that the content of P in all three species was the lowest(0.039–0.28 g kg),while N content was the highest(0.095–1.72 g kg).As the terminal organ of nutrient transport,the nutrient content of leaves was the highest.P.armandii(0.45 g kg) had a higher nutrient concentration than P.tabulaeformis(0.19 g kg) and P.crassifolia(0.29 g kg).The nutrient content of each species was highest in a young forest,but lowest in a mature forest.The nutrient content of all three tree species was significantly affected by soil nutrient content,and negatively correlated with available soil nutrients.

Ecological Structure and Prediction Equations for Estimating Tree Age, and Dendometric Parameters of <i>Acacia senegal</i>in the Senegalese Semi-Arid Zone—Ferlo

The development of equations to predict tree height, crown diameter, crown depth from stem diameter of a tree species enables arborists, researchers, and urban forest managers to model costs and benefits, analyze alternative management scenarios, and determine the best management practices for sustainable forests. The objective of this study was to develop regression prediction models for tree age, tree height, crown diameter, crown ratio and crown depth for A. senegal growing in Ferlo, in the northern Senegal. Four plantations of different years old (ISRA, 10 years old plantations, Ndodj, 8 years old plantations, Boulal, 5 years old plantations and Déali, 4 years old plantations) were selected. The following dendometric variables: crown height, crown diameter, stem diameter at the breast height, stem basal diameter (at 0.30 m) and the height from the tree base to first branch were measured on a total of 489 trees. The results suggested that the ecological structure of the different year old A. Senegal plantation revealed a bell-shaped form with left dissymmetric distribution indicating a predominance of individuals with small diameter at breast height. Allometry study of A. Senegal showed highly significant positive correlations (p = 0.00) between stem diameter at breast height, stem basal diameter, tree height, crown diameter and crown depth. Positive correlations were also found between crown diameter, tree height and crown height. Prediction models derived from these relationships can be used to estimate the tree height, stem diameter at breast height and crown depth from crown diameter with greater precision. As for A. Senegal age estimation, the established model is not strong as it can explain only 49.1% of the age variation.

Age-related changes of corneal endothelial cell in healthy Chinese tree shrew measured by non-contact specular microscope

AIM：To determine the impact of age on the morphology of endothelial cells and central corneal thickness（CCT） in Chinese tree shrew.METHODS：One-hundred and twenty eyes of 60 healthy Chinese tree shrews were studied.Based on age,the tree shrews were divided into four groups.After general anesthesia,the images of endothelium were acquired using non-contact specular microscope Topcon 3000 P.Eight parameters of corneal endothelial cells were measured by built-in software,including CCT,endothelial cell density（ECD）,percent hexagonality（HG%）,coeffi cient of variability（CV）,size of minimal cell（Smin）,size of maximal cell（Smax）,average cells size（Savg） and size standard deviation（Ssd）.Data were analyzed using STATA software.The differences of eight parameters among groups and correlations with age were analyzed.RESULTS：In all studied animals,the average CCT was 2 4 9.6 ± 2 .29 μ m（202-301 μm）,ECD was 308 0.72 ± 460.76 cells/mm^2（1239.6-4047.6 cells/mm^2） and CV was 29.10±7.60（13.6-54.6）.CV was significantly different among different groups（P〈0.001）.Strong correlation with age was found in ECD,Smax,Savg,Ssd and CV.CONCLUSION：Cornea of Chinese tree shrews had half CCT of human cornea and similar ECD,CV and size of corneal endothelial cells.Young adult tree shrews had higher ECD,HG% and low CV.ECD,Smax,Savg,Ssd and CV correlated with age signifi cantly.

Aging Mongolian pine plantations face high risks of drought-induced growth decline:evidence from both individual tree and forest stand measurements

Discerning vulnerability differences among different aged trees to drought-driven growth decline or to mortality is critical to implement age-specific countermeasures for forest management in water-limited areas.An important species for afforestation in dry environments of northern China,Mongolian pine(Pinus sylvestris var.mongolica Litv.)has recently exhibited growth decline and dieback on many sites,particularly pronounced in old-growth plantations.However,changes in response to drought stress by this species with age as well as the underlying mechanisms are poorly understood.In this study,tree-ring data and remotely sensed vegetation data were combined to investigate variations in growth at individual tree and stand scales for young(9-13 years)and aging(35-52 years)plantations of Mongolian pine in a water-limited area of northern China.A recent decline in tree-ring width in the older plantation also had lower values in satellited-derived normalized difference vegetation indices and normalized difference water indices relative to the younger plantations.In addition,all measured growth-related metrics were strongly correlated with the self-calibrating Palmer drought severity index during the growing season in the older plantation.Sensitivity of growth to drought of the older plantation might be attributed to more severe hydraulic limitations,as reflected by their lower sapwood-and leaf-specific hydraulic conductivities.Our study presents a comprehensive view on changes of growth with age by integrating multiple methods and provides an explanation from the perspective of plant hydraulics for growth decline with age.The results indicate that old-growth Mongolian pine plantations in water-limited environments may face increased growth declines under the context of climate warming and drying.

Characteristics of Soil Organic Carbon and Total Nitrogen in Rubber Plantations Soil at Different Age Stages in the Western Region of Hainan Island

[Objective]The aim was to study on the characteristics of soil organic carbon and nitrogen in rubber （Hevea brasiliensis Muell-Arg） plantations at different age stages in the western region of Hainan Island,so as to evaluate the ecological benefits of rubber plantations and provide basic data for studying the effect of tropical land utilization/cover change on the global carbon and nitrogen cycle. [Method]The situs was in Danzhou city,western region of Hainan Island,and the samples were four kinds of rubber plantations soil at different ages and one kind of control check （pepper,Piper nigrum L.） soil. In this research,four quadrats were set up in each sample,and the size of each was 20 cm×20 cm. Four specimens were gathered from four layers of 0-15,15-30,30-45,45-60,and the average of them was the last analysis result of each sample. Soil density was measured by cutting ring method,soil containing and hygroscopic water was detected by oven drying method,soil organic carbon （SOC） was measured by low temperature heated outside potassium dichromate oxidation-colorimetry method,and soil total nitrogen （STN） was detected by semimicro Kjeldahl method. [Result]SOC contents of different layers in rubber plantations soil at different age stages （including the CK pepper soil,the same as below） varied little,and the content of SOC in surface layer （0-15 cm） was higher,while the underlayer （45-60 cm） was lower than the average value; there was significant difference in SOC content among different kinds of soil,and the content was of 6.03-7.78 g/kg,tapping young trees （7 years） CK pepper mature age trees （30 years） prophase of young trees （2 years） tapping trees （16 years）; there was no significant difference in SOC storage among different kinds of soil,and the storage was of 61.33-74.29 t/hm2,mature age trees （30 years） tapping young trees （7 years） prophase of young trees （2 years） CK pepper tapping trees （16 years）; there was significant difference in STN content among rubber plantations soil at different age stages,the content was of 410.86-664.14 mg/kg2,CK pepper tapping young trees （7 years） prophase of young trees （2 years） mature age trees （30 years） tapping trees （16 years）,and STN content of tapping trees （16 years） soil was extremely lowest; there was significant difference in C/N ratio among different kinds of soil,the ratio was of 10.94-14.47,and the ratio of tapping trees （16 years） mature age trees （30 years） tapping young trees （7 years） CK pepper prophase of young trees （2 years）. [Conclusion]There wasn＇t unhealthy effect of rubber trees planted in tropical area on the content and storage of SOC,the content of STN and the ratio of C/N. there was no significant difference between rubber plantations and CK pepper soil,and the effects of rubber plantation on soil carbon-nitrogen was similar to that of other tropical crops （such as pepper）.

基于PAT TREE统计语言模型与关键词自动提取

未登录关键词的识别是中文信息处理中的一个关键问题。文章利用PAT TREE实现了一种可变长统计语言模型，由于不存在n元统计语言模型的截断效应，从而对待提取的关键词的长度没有限制。在该模型的基础上，通过相关性检测，从540M汉语语料中自动提取出了12万个关键词候选字串。最后，经过分析和筛选，候选字串的准确度由82．3％上升到96．1％。实验表明，基于PAT TREE的统计语言模型是实现未登录词提取的有力工具。

Stability evaluation of radial growth of Picea schrenkiana in different age groups in response to climate change in the eastern Tianshan Mountains

Global warming causes an unstable response in tree radial growth at high latitudes in the Northern Hemisphere.Additionally,different climatic responses of different age groups of trees have been found due to their different physiological mechanisms.In this study,the response stability and growth trend of three age groups(young<100 a,middle 100-200 a,old≥200 a)of Picea schrenkiana(Schrenk spruce)to climate change and the causes of the different responses in different age groups were analyzed in the relatively dry climate of the eastern Tianshan Mountains.The results showed that:(1)With the abrupt increase in temperature in 1989,the annual mean minimum temperature became the dominant radial growth-limiting factor of the three age groups of Schrenk spruce.(2)The radial growth of the middle and young groups was more sensitive than that of the old group based on growth-climate correlation analysis.(3)The radial growth of the different age groups had different responses to climate factors,and all age groups were unstable on time scales.(4)The trend of the linear regression simulation of the basal area increment(BAI)indicated that the Schrenk spruce had the same growth trends in different age groups with growth first increased and then decreased;however,the decreased growth rate was higher in the middle and young age groups than in the old age group after the abrupt increase in temperature.Therefore,we should pay active attention to the impact of drought on Schrenk spruce in the eastern Tianshan Mountains and should particularly strengthen the conservation and management of the middle and young age groups.

Response of plant,litter,and soil C:N:P stoichiometry to growth stages in Quercus secondary forests on the Loess Plateau,China

Ecological stoichiometry is an important indicator of biogeochemical cycles and nutrient limitations in terrestrial ecosystems.However,little is known about the response of ecological stoichiometry to plant growth.In this study,carbon(C),nitrogen(N),and phosphorus(P)concentrations were evaluated in plant tissues(trees,shrubs,and herbs),litter,and soil of young(≤40-year-old),middle-aged(41–60-year-old),near-mature(61–80-year-old),and mature(81–120-year-old)Quercus secondary forests on the Loess Plateau,China.Vegetation composition,plant biomass,and C stock were determined to illustrate their interaction with stoichiometry.Only tree biomass C signifi cantly increased with stand development.Leaf N and trunk P concentrationsgenerally increased,but branch P decreased with growth stage.Fine roots had the highest C and P concentrations at the middle-aged stage.In contrast,shrubs,herbs,litter,and soil C:N:P stoichiometry did not change signifi cantly during stand development.Leaf N and P were positively correlated with soil C,N,P,and their ratios.However,there was no signifi cant correlation between litter and leaves in terms of C:N:P stoichiometry.A redundancy analysis showed that soil N best explained leaf N and P variance,and tree biomass and C stock were related to biotic factors such as tree age and shrub biomass.Hierarchical partitioning analysis indicated that,compared with soil or litter variables,stand age only accounted for a relatively small proportion of leaf C,N,and P variation.Thus,secondary Quercus ecosystems might have inherent ability to maintain sensitive responses of metabolically active organs to environmental factors during stand aging.The results of this work help to elucidate the biogeochemical cycling of secondary forest ecosystems in tree development,provide novel insights into the adaptation strategies of plants in diff erent organs and growth stages,and could be used to guide fertilization programs and optimize forest structure.

肿瘤抗原MAGE-A家族成员进化关系的初步研究

目的：探讨肿瘤抗原MAGE-A家族成员间的同源性，发现公共CTL表位对抗肿瘤逃逸。方法：采用1999DNNAstar软件Windwow95/98平台支持下的蛋白质核酸序列编辑、分析工具对MAGE-A家族成员进行开放阅读框、蛋白及其CTL序列同源性分析井构建种系发生树。结果：表明MAGE-A家族核苷酸同源性高达57．6％-98.0％，种系发生树显示各成员来源于同一祖先井在不同时间分野，且部分CIL表位高度相似。结论：MAGE-A 家族成员来自于同一祖先的同一家族，在肿瘤细胞中表达存在异质性，故此研究结果将有利于在发展肿瘤治疗性细胞免疫疫苗中选择合适的CTL表位。

Study on Anticipant Mature Age of Pinus tabuliformis in Shandong Province

Through analysis on materials, the fitting empirical equation for growth process of Pinus tabuliformis was obtained, i.e. , the function with increment as variable and time as the independent variable. The mature age of P. tabuliformis was obtained by mathematical operation. The mature age of forest plantation was determined as 61 a, and that of public welfare forest was 81 a. And P. tabuliformis age groups were divided according to related technical requirements.

Activities of some defense proteins associated with age or plant family in larch needles of Larix olgensis and L. kaempferi× L. gmelinii

Defence proteins are a kind of chemical defence compounds. They play a key role in plant restraining biotic and abiotic harm. To illuminate activities of some defense proteins associated with age or plant family of larch, the larch needles were collected from two different families of Korean larch, Larix olgensis and a hybrid larch, L. kaempferi × L. gmelinii, respectively, and then the activities of defence proteins in those were tested using a UV spectrophotometry. The results showed that the activities of protective enzymes at thel6-/17- and 19-year-age groups were higher than those at the other age groups in the both larch species. While the activities of polyphenol oxidase （PPO） at the 16-/17-year-age group and phenylalanine ammonia-lyase （PAL） and protease inhibitors at the 19-year-age group were the highest among all age groups. Then, compared with L. olgensis, the hybrid larch had significant effects on the activities of protective enzymes. The effects of plant family on the activities of PAL and chymotrypsin inhibitor were significantly different, and then those on the activities of PPO were not significantly different. The effects of the two families in L. olgensis on the activities of trypsin inhibitor （TI） were significantly different, while those in the hybrid larch on the activities of TI were contrary. To conclusions showed that the different age and plant family significantly affected the activities of defence proteins in the needles of two larch species, and then enhanced the larch resistance to pests. These could play a key function in forestry tree genetic improvement and management in future.

Study on Expected Mature Age of Individual Ailanthus altissima

This study was conducted on the analytic tree and got the fitting empirical equation of tree growth,in which the tree growth rate was used as the variable and time as the independent variable.The arithmetical operation to the function got the mature age of tree growth,and the expected mature age of Ailanthus altissima was 21 a.In addition,the application as well as the research direction and matters needing attention were proposed.

Study on the Practical Mature Age of Individual Platycladus orientalis

This study was conducted on the analytic tree and got the fitting empirical equation of tree growth, in which the tree growth rate was used as the variable and time as the independent variable. The arithmetical operation to the function got the mature age of tree growth, and the mature ages of the timer forest, the protection forest and the special-purpose forest of Platycladus orientalis were 71, 111 and 141 a, respectively. In addition, the application as well as the research direction and matters needing attention were proposed.

Ants Assemblage Method According to an Age Gradient of Mango Orchards in Korhogo (C&ocirc;te d’Ivoire)

This study was conducted in the Korhogo region in an age gradient of mango orchards. The goal was to assess diversity and determine the influence of the environment on the composition of these organisms. Sampling was done using pitfall traps, threshing and manual collection. A total of 82 ant species were sampled on all plots. The cultivated plots are richer in ant species than the natural environment. Of the three (3) sampling methods, the Manual Collection proved to be more efficient (eff = 79.52%), followed by threshing (eff = 75.15%) and finally the least effective trap pit (eff = 73.39%). The number of individuals of the species varies according to the environment. The highest value was obtained in the young plot (485 individuals), followed by the intermediate plot (478 individuals), then the older plot (426 individuals) and finally the control plot (320 individuals). The species Camponotus acvapimensis was harvested mainly with 159 individuals. On the other hand, the species Lepisiota sp.1;Camponotus rufigenis, Camponotus sericeus and Oecophylla longinoda were specifically harvested in the control, young, intermediate and aged plots, respectively.

Study on the Practical and Ideal Mature Age of Pinus densiflora

This study was conducted on the analytic tree and got the fitting empirical equation of tree growth, in which the tree growth rate was used as the variable and time as the independent variable. The arithmetical operation to the function got the mature age of tree growth, and the practical and ideal mature ages of Pinus densiflora were 58 and 123.5 a, respectively. In addition, the application research direction and matters needing attention were proposed.

Study on Practical Mature Age of Individual Pinus thunbergii×P. densiflora

This study was conducted on the analytic tree and got the fitting empirical equation of tree growth, in which the tree increment was used as the variable and time as the independent variable. The arithmetical operation to the function gave the mature age of tree growth, and the practical mature age of Pinus thunbergii×P. densiflora was 41 a. In addition, the application as well as the research direction and matters needing attention were proposed.

面向岗位能力匹配的多关键词编码MPB-Tree索引技术

单一关键词筛选的求职者信息与对应岗位能力的匹配度具有较大偏差,为帮助企业人力资源管理部门准确、全面地完成人才筛选,提出了一种多关键词编码MPB-Tree索引技术。扩展处理核心关键词,并构建岗位能力需求关键词轮廓集合。结合PB-Tree与Patricia索引建立支持多关键词索引的MPB-Tree索引结构。基于MPB-Tree索引结果与关键词轮廓集合的相关度,索引岗位能力需求关键词,实现岗位能力匹配。结果表明,所提技术的查全率和查准率均高于单一核心关键词索引下的查全率和查准率,由此说明所提技术的索引结果更为全面和准确。

集合空间关键字内聚组查询方法

给定一个道路网络和社交网络,集合空间关键字查询的目的是找到一组兴趣点,该组兴趣点的文本信息包含所有查询关键字,与查询的位置较近且彼此之间的距离较小。内聚组查询的目的是找到在地理位置和社交关系上紧密联系的一组用户;而集合空间关键字内聚组查询的目的是找到满足查询要求的一对最佳匹配的兴趣点集合和用户集合。针对这一问题,提出一种新的集合空间关键字内聚组查询处理模式。首先通过快速贪心查询过程获得候选兴趣点集合,然后使用core-tree结构存储(k,c)-core核心分解的结果,从而提高内聚组查询效率,并且保证查询结果能够同时满足用户之间的社会关系约束和兴趣点之间的空间位置约束。通过在真实数据集上开展实验,结果表明提出的方法比枚举方法的查询效率快1~2个数量级,并且具有较高查询准确性。