This study aimed to examine the number of circulating Toll-like receptor 4（TLR4） ＋ CD14＋ monocytes in patients with different stages of chronic kidney disease（CKD）, their responses to lipopolysaccharide（LPS）, ...This study aimed to examine the number of circulating Toll-like receptor 4（TLR4） ＋ CD14＋ monocytes in patients with different stages of chronic kidney disease（CKD）, their responses to lipopolysaccharide（LPS）, and to explore the potential association of the number of TLR4＋CD14＋ monocytes with clinical laboratory measures. The numbers of TLR4＋CD14＋, LPS-stimulated TNF-？＋CD14＋ and interleukin（IL）-6＋CD14＋ monocytes were determined by flow cytometry in 9 patients with stage 3 CKD, 11 with stage 4 CKD, 16 with stage 5 CKD, and 19 healthy controls（HCs）. Their laboratory tests were performed by routine methods and the potential association among these measures was analyzed by Pearson＇s correlation analysis. The numbers of CD14＋, CD14＋TLR4＋, LPSstimulated TNF-？＋CD14＋ and IL-6＋CD14＋ monocytes in patients with CKD were significantly less than those of HCs（all P〈0.05）, and were negatively associated with patient disease severity. The number of CD14＋TLR4＋ monocytes was positively correlated with estimated glomerular filtration rate（e GFR, P〈0.001） and the levels of hematocrit（P〈0.01）, but negatively correlated with the levels of blood urine nitrogen, serum creatinine, and C-reactive protein（P〈0.001 for all）, in the CKD patients. Our data indicate that significant reduction in the number of TLR4＋ monocytes and their impaired responses to LPS may be associated with the progression of CKD in Chinese patients.展开更多
文摘This study aimed to examine the number of circulating Toll-like receptor 4（TLR4） ＋ CD14＋ monocytes in patients with different stages of chronic kidney disease（CKD）, their responses to lipopolysaccharide（LPS）, and to explore the potential association of the number of TLR4＋CD14＋ monocytes with clinical laboratory measures. The numbers of TLR4＋CD14＋, LPS-stimulated TNF-？＋CD14＋ and interleukin（IL）-6＋CD14＋ monocytes were determined by flow cytometry in 9 patients with stage 3 CKD, 11 with stage 4 CKD, 16 with stage 5 CKD, and 19 healthy controls（HCs）. Their laboratory tests were performed by routine methods and the potential association among these measures was analyzed by Pearson＇s correlation analysis. The numbers of CD14＋, CD14＋TLR4＋, LPSstimulated TNF-？＋CD14＋ and IL-6＋CD14＋ monocytes in patients with CKD were significantly less than those of HCs（all P〈0.05）, and were negatively associated with patient disease severity. The number of CD14＋TLR4＋ monocytes was positively correlated with estimated glomerular filtration rate（e GFR, P〈0.001） and the levels of hematocrit（P〈0.01）, but negatively correlated with the levels of blood urine nitrogen, serum creatinine, and C-reactive protein（P〈0.001 for all）, in the CKD patients. Our data indicate that significant reduction in the number of TLR4＋ monocytes and their impaired responses to LPS may be associated with the progression of CKD in Chinese patients.
