<p style="text-align:justify;"> <i><span style="font-family:Verdana;">Background</span></i><span style="font-family:;" "=""> <i>&l...<p style="text-align:justify;"> <i><span style="font-family:Verdana;">Background</span></i><span style="font-family:;" "=""> <i><span style="font-family:Verdana;">and</span></i> <i><span style="font-family:Verdana;">aim</span></i><span style="font-family:Verdana;">: Hepatorenal toxicity is a very common ailment with </span><span style="font-family:Verdana;">resultant</span><span style="font-family:Verdana;"> deleterious burden on the overall body systems and high mortality rate. Although myriads of drug agents are in circulation, its medical management is still inadequate as no effective treatment which inhibits disease progression and </span><span style="font-family:Verdana;">complications,</span> <span style="font-family:Verdana;">has</span><span style="font-family:Verdana;"> been </span><span style="font-family:Verdana;">synthesized</span><span style="font-family:Verdana;"> yet. Therefore, this study focused on the potentials of </span><i><span style="font-family:Verdana;">Kigelia</span></i> <i><span style="font-family:Verdana;">africana</span></i><span style="font-family:Verdana;"> ethanolic leaf extract (KAELE) in preventing hepatorenal toxicity using </span><span><span style="font-family:Verdana;">CCl</span><sub><span style="font-family:Verdana;">4</span></sub></span><span style="font-family:Verdana;"> model of toxicity in rats. </span><i><span style="font-family:Verdana;">Method:</span></i><span style="font-family:Verdana;"> KAELE was subjected to phytochemical screening. Following two-week acclimatization, </span><span style="font-family:Verdana;">thirty-six</span><span style="font-family:Verdana;"> (N = 36) adult male Wistar rats were grouped into six consisting of six animals each (n = 6). Group I was given distilled water as control while groups II to VI received silymarin (100 mg/kg), CCl</span><sub><span style="font-family:Verdana;">4</span></sub><span style="font-family:Verdana;"> (1 ml/kg), KAELE (100 mg/kg, 200 mg/kg </span><span style="font-family:Verdana;">and</span><span style="font-family:Verdana;"> 400 mg/kg) respectively. All groups pre-treated with silymarin and </span></span><i><span style="font-family:Verdana;">Kigelia</span></i><span style="font-family:;" "=""> <i><span style="font-family:Verdana;">africana</span></i><span style="font-family:Verdana;"> ethanol leaf extract lasted for a period of fourteen (14) days using a gastric tube. CCl</span><sub><span style="font-family:Verdana;">4</span></sub><span style="font-family:Verdana;"> was administered intraperitoneally to groups II, III, IV, V </span><span style="font-family:Verdana;">and</span><span style="font-family:Verdana;"> VI 48 hours after the last pretreatment on day 14. </span><span style="font-family:Verdana;">Post treatment</span><span style="font-family:Verdana;">, animals were sacrificed and the blood obtained and sera </span><span style="font-family:Verdana;">used</span><span style="font-family:Verdana;"> for biochemical analysis while the tissues for histological evaluations. </span><i><span style="font-family:Verdana;">Results</span></i><span style="font-family:Verdana;">: </span></span><span style="font-family:;" "=""><span style="font-family:Verdana;">The phytochemical tests revealed the presence of flavonoids, tannins, steroids, terpenoids, saponins, glycosides, alkaloids, and phenols. There was a significant decrease (P < 0.05) in the level of all serum liver enzymes (AST, ALT </span><span style="font-family:Verdana;">and</span><span style="font-family:Verdana;"> ALP) in the </span><span style="font-family:Verdana;">extract-treated</span><span style="font-family:Verdana;"> groups. KAELE showed a dose-dependent </span><span style="font-family:Verdana;">hepato-protective</span><span style="font-family:Verdana;"> property as it significantly mitigated the effects of carbon tetrachloride on the liver function markers studied (total bilirubin, conjugated bilirubin, albumin </span><span style="font-family:Verdana;">and</span><span style="font-family:Verdana;"> total protein). KAELE showed </span><span style="font-family:Verdana;">the decrease</span><span style="font-family:Verdana;"> necrotic hepatic plates around the portal areas and damaged blood vessels with less fatty </span><span style="font-family:Verdana;">acids</span><span style="font-family:Verdana;"> infiltrations in this study. </span><i><span style="font-family:Verdana;">Conclusion:</span></i><span style="font-family:Verdana;"> KAELE possesses hepatorenal protective potentials.</span></span> </p>展开更多
This paper reports the beneficial effect of twice daily ingestion (a table spoonful) of dried Kigelia africana fruit powder in the management of Polycystic Ovary Syndrome (PCOS) in two patients. Both patients had the ...This paper reports the beneficial effect of twice daily ingestion (a table spoonful) of dried Kigelia africana fruit powder in the management of Polycystic Ovary Syndrome (PCOS) in two patients. Both patients had the classical triad of Amenorrhoea, acne and hirsutism. The two were 25 years old and 22 years old spinsters respectively. The ultrasonography was suggestive only in the latter;unfortunately there were no facilities to do the confirmatory serum enzymes assay. The use of herbal preparation restored the menstrual flow in both of them as well as leading to significant reduction in the acne but there was no noticeable effect on the hirsutism. There was no observable side effect associated with the use of the powder. These preliminary data thus suggest that Kigelia africana fruit powder may be beneficial for cases of PCOS especially in the developing countries where the new generation oral contraceptives, presently being used for the condition, may not be readily available.展开更多
Objective: Kigelia africana, a tropical tree, which has long been used in African traditional medicine. The objective of the current study has been identifying the constituents of K. africana and verifying its utiliti...Objective: Kigelia africana, a tropical tree, which has long been used in African traditional medicine. The objective of the current study has been identifying the constituents of K. africana and verifying its utilities in traditional medicine. Materials and Methods: The methanol extract of K. africana fruits was subjected to chromatographic fractionation utilizing different techniques. The methanol extract together with the isolated compounds were tested for their bioactivities in a series of cell-based assays. Results: The current work led to isolation and characterization of nine constituents including iridoid glycosides, phenylpropanoid derivatives, and a eucommiol derivative. The hexanes extract caused inhibition of the opportunistic yeast; Cryptococcus neoformans Pinh. The chloroform extract exhibited substantial antileishmanial activity of Leishmania donovani. Verminoside(1) showed weak inhibition of the CB1, CB2, and Kappa opioid receptors. Compound 4 exhibited weak inhibition of the Kappa and Mu opioid receptors. The hexanes and the chloroform extracts of K. africana exhibited inhibitory activity against the pathogenic parasite Trypanosoma brucei. The ethyl acetate extract showed the same activity. Conclusions: This is the first report on the isolation of coniferyl 4-0-(3-D-glucopyranoside(7), a eucommiol derivative(crescentin IV)(6), and 6-feruloylcatalpol(4) from the genus Kigelia. It is also the first report on the separation of ajugol(2), catalpol(3), and specioside(5) from the fruits of K. africana. Revision of the^1 H and ^(13)C-NMR spectra of 6-feruloylcatalop(4) and 6-p-hydroxycinnamoylcatalpol(5, specioside) is described. Further, the results of the in vitro assays corroborate the traditional utility of this plant in medicine.展开更多
文摘<p style="text-align:justify;"> <i><span style="font-family:Verdana;">Background</span></i><span style="font-family:;" "=""> <i><span style="font-family:Verdana;">and</span></i> <i><span style="font-family:Verdana;">aim</span></i><span style="font-family:Verdana;">: Hepatorenal toxicity is a very common ailment with </span><span style="font-family:Verdana;">resultant</span><span style="font-family:Verdana;"> deleterious burden on the overall body systems and high mortality rate. Although myriads of drug agents are in circulation, its medical management is still inadequate as no effective treatment which inhibits disease progression and </span><span style="font-family:Verdana;">complications,</span> <span style="font-family:Verdana;">has</span><span style="font-family:Verdana;"> been </span><span style="font-family:Verdana;">synthesized</span><span style="font-family:Verdana;"> yet. Therefore, this study focused on the potentials of </span><i><span style="font-family:Verdana;">Kigelia</span></i> <i><span style="font-family:Verdana;">africana</span></i><span style="font-family:Verdana;"> ethanolic leaf extract (KAELE) in preventing hepatorenal toxicity using </span><span><span style="font-family:Verdana;">CCl</span><sub><span style="font-family:Verdana;">4</span></sub></span><span style="font-family:Verdana;"> model of toxicity in rats. </span><i><span style="font-family:Verdana;">Method:</span></i><span style="font-family:Verdana;"> KAELE was subjected to phytochemical screening. Following two-week acclimatization, </span><span style="font-family:Verdana;">thirty-six</span><span style="font-family:Verdana;"> (N = 36) adult male Wistar rats were grouped into six consisting of six animals each (n = 6). Group I was given distilled water as control while groups II to VI received silymarin (100 mg/kg), CCl</span><sub><span style="font-family:Verdana;">4</span></sub><span style="font-family:Verdana;"> (1 ml/kg), KAELE (100 mg/kg, 200 mg/kg </span><span style="font-family:Verdana;">and</span><span style="font-family:Verdana;"> 400 mg/kg) respectively. All groups pre-treated with silymarin and </span></span><i><span style="font-family:Verdana;">Kigelia</span></i><span style="font-family:;" "=""> <i><span style="font-family:Verdana;">africana</span></i><span style="font-family:Verdana;"> ethanol leaf extract lasted for a period of fourteen (14) days using a gastric tube. CCl</span><sub><span style="font-family:Verdana;">4</span></sub><span style="font-family:Verdana;"> was administered intraperitoneally to groups II, III, IV, V </span><span style="font-family:Verdana;">and</span><span style="font-family:Verdana;"> VI 48 hours after the last pretreatment on day 14. </span><span style="font-family:Verdana;">Post treatment</span><span style="font-family:Verdana;">, animals were sacrificed and the blood obtained and sera </span><span style="font-family:Verdana;">used</span><span style="font-family:Verdana;"> for biochemical analysis while the tissues for histological evaluations. </span><i><span style="font-family:Verdana;">Results</span></i><span style="font-family:Verdana;">: </span></span><span style="font-family:;" "=""><span style="font-family:Verdana;">The phytochemical tests revealed the presence of flavonoids, tannins, steroids, terpenoids, saponins, glycosides, alkaloids, and phenols. There was a significant decrease (P < 0.05) in the level of all serum liver enzymes (AST, ALT </span><span style="font-family:Verdana;">and</span><span style="font-family:Verdana;"> ALP) in the </span><span style="font-family:Verdana;">extract-treated</span><span style="font-family:Verdana;"> groups. KAELE showed a dose-dependent </span><span style="font-family:Verdana;">hepato-protective</span><span style="font-family:Verdana;"> property as it significantly mitigated the effects of carbon tetrachloride on the liver function markers studied (total bilirubin, conjugated bilirubin, albumin </span><span style="font-family:Verdana;">and</span><span style="font-family:Verdana;"> total protein). KAELE showed </span><span style="font-family:Verdana;">the decrease</span><span style="font-family:Verdana;"> necrotic hepatic plates around the portal areas and damaged blood vessels with less fatty </span><span style="font-family:Verdana;">acids</span><span style="font-family:Verdana;"> infiltrations in this study. </span><i><span style="font-family:Verdana;">Conclusion:</span></i><span style="font-family:Verdana;"> KAELE possesses hepatorenal protective potentials.</span></span> </p>
文摘This paper reports the beneficial effect of twice daily ingestion (a table spoonful) of dried Kigelia africana fruit powder in the management of Polycystic Ovary Syndrome (PCOS) in two patients. Both patients had the classical triad of Amenorrhoea, acne and hirsutism. The two were 25 years old and 22 years old spinsters respectively. The ultrasonography was suggestive only in the latter;unfortunately there were no facilities to do the confirmatory serum enzymes assay. The use of herbal preparation restored the menstrual flow in both of them as well as leading to significant reduction in the acne but there was no noticeable effect on the hirsutism. There was no observable side effect associated with the use of the powder. These preliminary data thus suggest that Kigelia africana fruit powder may be beneficial for cases of PCOS especially in the developing countries where the new generation oral contraceptives, presently being used for the condition, may not be readily available.
文摘Objective: Kigelia africana, a tropical tree, which has long been used in African traditional medicine. The objective of the current study has been identifying the constituents of K. africana and verifying its utilities in traditional medicine. Materials and Methods: The methanol extract of K. africana fruits was subjected to chromatographic fractionation utilizing different techniques. The methanol extract together with the isolated compounds were tested for their bioactivities in a series of cell-based assays. Results: The current work led to isolation and characterization of nine constituents including iridoid glycosides, phenylpropanoid derivatives, and a eucommiol derivative. The hexanes extract caused inhibition of the opportunistic yeast; Cryptococcus neoformans Pinh. The chloroform extract exhibited substantial antileishmanial activity of Leishmania donovani. Verminoside(1) showed weak inhibition of the CB1, CB2, and Kappa opioid receptors. Compound 4 exhibited weak inhibition of the Kappa and Mu opioid receptors. The hexanes and the chloroform extracts of K. africana exhibited inhibitory activity against the pathogenic parasite Trypanosoma brucei. The ethyl acetate extract showed the same activity. Conclusions: This is the first report on the isolation of coniferyl 4-0-(3-D-glucopyranoside(7), a eucommiol derivative(crescentin IV)(6), and 6-feruloylcatalpol(4) from the genus Kigelia. It is also the first report on the separation of ajugol(2), catalpol(3), and specioside(5) from the fruits of K. africana. Revision of the^1 H and ^(13)C-NMR spectra of 6-feruloylcatalop(4) and 6-p-hydroxycinnamoylcatalpol(5, specioside) is described. Further, the results of the in vitro assays corroborate the traditional utility of this plant in medicine.