Dendrobium officinale Kimura&Migo(D.officinale)is a common,high medicinal value food and herbal medicine used in the treatment of yin-deficient diseases.Adulterants obtained with similar inexpensive species of Den...Dendrobium officinale Kimura&Migo(D.officinale)is a common,high medicinal value food and herbal medicine used in the treatment of yin-deficient diseases.Adulterants obtained with similar inexpensive species of Dendrobium plants are available in the market.However,Dendrobium species with different quality levels or from different regions as well as analogous species have a similar appearance,which makes the discrimination of Dendrobium species difficult.Reliable objective methods for authenticating D.officinale are lacking.Here,samples of Dendrobium officinale Kimura&Migo(D.officinale)from two regions with three quality levels and sample of the analogous species Dendrobium compactum Rolfe ex W.Hackett(D.compactum)were subjected to genome-wide biological response fingerprinting(BioReF)in RAW264.7 cells by RNA sequencing.The biological pathways affected by these samples could distinguish D.officinale from D.compactum.The high-quality D.officinale samples from Yunnan exhibited the strongest inhibition of lipopolysaccharide(LPS)induced inflammatory genes and pathways,followed by samples from Myanmar.The medium-and low-quality samples from Yunnan exhibited weaker effects,and D.compactum exerted mild effects.The RNA sequencing results were strengthened by the results from analyses of the expression of the inflammation-related genes Il6,Ccl5,and Cox2 and the production of nitric oxide.Thus,transcriptomic profiling can be used to evaluate the quality of D.officinale and distinguish its origin.展开更多
[Objective] The paper was to understand the control efficacy and the optimal dosage of 16% validamycin？thifluzamide SC on controlling Selerotium rolfsii in Dendrobium officinale Kimura et Migo. [Method] The contr...[Objective] The paper was to understand the control efficacy and the optimal dosage of 16% validamycin？thifluzamide SC on controlling Selerotium rolfsii in Dendrobium officinale Kimura et Migo. [Method] The control efficacy of 16% validamycin·thifluzamide SC against S.rolfsii was determined by spraying agents on the whole plant of D.officinale. The significance of differences was analyzed by Duncan＇s new multiple range test （DMRT）. [Result] The control efficacy of 16% validamycin.thifluzamide SC sprayed at the doses of 80-160 mg a.i./kg was 85.33% at 14 d post spraying, higher than that of control agent 4% validamycin AS sprayed at the dose of 80 mg a.i./kg （78.36%）, and equivalent to that of control agent 240 g/L thifluzamide SC sprayed at the dose of 120 mg a.i./kg （90.60%）. [Conclusion] 16% Validamycin·thifluzamide SC sprayed at the doses of 80-160 mg a.i./kg could effectively control the damage of S.rolfsii in D.officinale, and could be popularized in the production.展开更多
[Objectives] To observe and compare the clinical effect and safety of Dendrobium officinale Kimura et Migo from Longhushan on treatment of type 2 diabetes. [Methods] 90 patients with type 2 diabetes were randomly divi...[Objectives] To observe and compare the clinical effect and safety of Dendrobium officinale Kimura et Migo from Longhushan on treatment of type 2 diabetes. [Methods] 90 patients with type 2 diabetes were randomly divided into group A,group B and control group by randomized control method. Group A adopted D. officinale Kimura et Migo and metformin hydrochloride; group B adopted D. officinale Kimura et Migo; the control group adopted metformin hydrochloride. The fasting plasma glucose( FPG) of 3 groups was measured before the treatment and 2 weeks,4 weeks,6 weeks,8 weeks,10 weeks,and 12 weeks after the treatment,Hb A1 c was measured and diabetes symptom grade score was counted before the treatment and 12 weeks after treatment,observation indicators and clinical effect of 3 groups of patients were compared. [Results] The fasting plasma glucose level in group A was lower than that in other 2 groups,the difference was statistically significant( P < 0. 01),and there was no statistical significance in the difference between group B and the control group( P >0. 05); Hb A1 c of group A for 12 weeks after treatment was lower than that of other 2 groups,the difference was statistically significant( P < 0. 01),and there was no statistical significance in the difference between group B and the control group( P > 0. 05); the difference in diabetes symptom grade score of group A was greater than that in other 2 groups,the difference was statistically significant( P < 0. 01),and there was no statistical significance in the difference between group B and the control group( P > 0. 05). [Conclusions]D. officinale Kimura et Migo from Longhushan can effectively reduce the fasting plasma glucose level and Hb A1 c of type 2 diabetic patients and effectively improve the symptoms of patients; clinical application of D. officinale Kimura et Migo has equivalent effect with metformin hydrochloride,and combination of D. officinale Kimura et Migo with metformin hydrochloride has better effect than single use.展开更多
中药石斛基原复杂,其主流品种有霍山石斛Dendrobium huoshanense C.Z.Tang et S.J.Cheng、铁皮石斛D.officinale Kimura et Migo、金钗石斛D.nobile Lindl.,《中华人民共和国药典》(以下简称《中国药典》)2005年版石斛项下增加了“及其...中药石斛基原复杂,其主流品种有霍山石斛Dendrobium huoshanense C.Z.Tang et S.J.Cheng、铁皮石斛D.officinale Kimura et Migo、金钗石斛D.nobile Lindl.,《中华人民共和国药典》(以下简称《中国药典》)2005年版石斛项下增加了“及其近似种”的描述,难以确定具体物种,有必要对其进一步正本清源。对石斛属的起源进行本草考证,对霍山石斛、铁皮石斛、金钗石斛的本草起源进行梳理,为不同石斛属药材的应用提供参考。霍山石斛是最早作为中药石斛应用的品种,高效液相色谱法特征图谱等方法为《中国药典》2020年版收录霍山石斛提供了技术支撑;铁皮石斛应为石斛药材中应用时间最久、范围最广的品种,其主要为浙江、云南、贵州,以及广东、广西、福建、江西等地丹霞地貌上生长的种群,应高度重视并对其进行保护;D.nobile在历代文献中的中文名称混乱,应明确将其作为金钗石斛的拉丁学名。枫斗是我国名贵中药和传统保健品,起源于霍山石斛。古代名医对霍山石斛、铁皮石斛、金钗石斛、川石斛的用法各有特色,建议厘清品种来源、功效,加强现代研究,在修订《中国药典》时将其各自单列,充分发挥中药石斛的临床价值。展开更多
为探究12种必需矿质元素(包括N、P、K、Ca、Mg、S、Fe、Mn、Cu、Zn、B、Mo)对铁皮石斛(Dendrobium officinale Kimura et Migo)生长及生理指标的影响,以铁皮石斛3个月组培苗为材料,以MS培养基为基础设置全营养对照(CK)、单一缺素处理和...为探究12种必需矿质元素(包括N、P、K、Ca、Mg、S、Fe、Mn、Cu、Zn、B、Mo)对铁皮石斛(Dendrobium officinale Kimura et Migo)生长及生理指标的影响,以铁皮石斛3个月组培苗为材料,以MS培养基为基础设置全营养对照(CK)、单一缺素处理和缺素恢复处理,分析各处理条件下铁皮石斛的表型特征和生理变化。结果显示:缺N、P、Ca、Cu、Zn处理组铁皮石斛组培苗缺素症状明显,整株的生长状态及叶片和假鳞茎的解剖结构均受到影响;除Zn外,其他元素缺素恢复处理后症状均得到明显改善。缺N、K、Ca、S、Zn处理组假鳞茎长及缺N、Ca处理组的叶片数受抑制明显。除P外,其他元素缺素处理组叶片叶绿素含量明显降低,多数缺素恢复处理组叶片叶绿素a和叶绿素b含量较缺素处理组极显著(P<0.01)或显著(P<0.05)升高;缺Fe处理组的叶绿素a含量与叶绿素b含量的比值明显降低,恢复处理后接近于CK。除Fe外,其他微量元素缺素处理对叶片超氧化物歧化酶(SOD)活性抑制明显,而缺大量元素处理组的SOD活性均不同程度升高;缺P处理组的过氧化物酶(POD)活性明显降低,而缺N、Mg、S、Mn、Zn、Cu处理组的POD活性明显升高,除Mn外,其他元素缺素恢复处理组的POD活性均升高。缺N、P可大幅提高叶片可溶性糖含量,缺N、Mn、Cu可大幅提高叶片可溶性蛋白质含量。缺N、Cu处理组假鳞茎多糖含量大幅高于CK,Ca、Mo缺素恢复处理组假鳞茎多糖含量较缺素处理组极显著升高;缺Mg处理组假鳞茎总黄酮含量大幅高于CK。综上,在保证铁皮石斛正常生长的前提下,可通过Ca、Mo缺素恢复处理来提高铁皮石斛假鳞茎多糖含量,通过适量缺Mg处理来提高铁皮石斛假鳞茎总黄酮含量。展开更多
基金the Beijing Talents Foundation(Grant number 2017000021223ZK30)the National Natural Science Foundation of China(Grant number 61927819).
文摘Dendrobium officinale Kimura&Migo(D.officinale)is a common,high medicinal value food and herbal medicine used in the treatment of yin-deficient diseases.Adulterants obtained with similar inexpensive species of Dendrobium plants are available in the market.However,Dendrobium species with different quality levels or from different regions as well as analogous species have a similar appearance,which makes the discrimination of Dendrobium species difficult.Reliable objective methods for authenticating D.officinale are lacking.Here,samples of Dendrobium officinale Kimura&Migo(D.officinale)from two regions with three quality levels and sample of the analogous species Dendrobium compactum Rolfe ex W.Hackett(D.compactum)were subjected to genome-wide biological response fingerprinting(BioReF)in RAW264.7 cells by RNA sequencing.The biological pathways affected by these samples could distinguish D.officinale from D.compactum.The high-quality D.officinale samples from Yunnan exhibited the strongest inhibition of lipopolysaccharide(LPS)induced inflammatory genes and pathways,followed by samples from Myanmar.The medium-and low-quality samples from Yunnan exhibited weaker effects,and D.compactum exerted mild effects.The RNA sequencing results were strengthened by the results from analyses of the expression of the inflammation-related genes Il6,Ccl5,and Cox2 and the production of nitric oxide.Thus,transcriptomic profiling can be used to evaluate the quality of D.officinale and distinguish its origin.
文摘[Objective] The paper was to understand the control efficacy and the optimal dosage of 16% validamycin？thifluzamide SC on controlling Selerotium rolfsii in Dendrobium officinale Kimura et Migo. [Method] The control efficacy of 16% validamycin·thifluzamide SC against S.rolfsii was determined by spraying agents on the whole plant of D.officinale. The significance of differences was analyzed by Duncan＇s new multiple range test （DMRT）. [Result] The control efficacy of 16% validamycin.thifluzamide SC sprayed at the doses of 80-160 mg a.i./kg was 85.33% at 14 d post spraying, higher than that of control agent 4% validamycin AS sprayed at the dose of 80 mg a.i./kg （78.36%）, and equivalent to that of control agent 240 g/L thifluzamide SC sprayed at the dose of 120 mg a.i./kg （90.60%）. [Conclusion] 16% Validamycin·thifluzamide SC sprayed at the doses of 80-160 mg a.i./kg could effectively control the damage of S.rolfsii in D.officinale, and could be popularized in the production.
文摘[Objectives] To observe and compare the clinical effect and safety of Dendrobium officinale Kimura et Migo from Longhushan on treatment of type 2 diabetes. [Methods] 90 patients with type 2 diabetes were randomly divided into group A,group B and control group by randomized control method. Group A adopted D. officinale Kimura et Migo and metformin hydrochloride; group B adopted D. officinale Kimura et Migo; the control group adopted metformin hydrochloride. The fasting plasma glucose( FPG) of 3 groups was measured before the treatment and 2 weeks,4 weeks,6 weeks,8 weeks,10 weeks,and 12 weeks after the treatment,Hb A1 c was measured and diabetes symptom grade score was counted before the treatment and 12 weeks after treatment,observation indicators and clinical effect of 3 groups of patients were compared. [Results] The fasting plasma glucose level in group A was lower than that in other 2 groups,the difference was statistically significant( P < 0. 01),and there was no statistical significance in the difference between group B and the control group( P >0. 05); Hb A1 c of group A for 12 weeks after treatment was lower than that of other 2 groups,the difference was statistically significant( P < 0. 01),and there was no statistical significance in the difference between group B and the control group( P > 0. 05); the difference in diabetes symptom grade score of group A was greater than that in other 2 groups,the difference was statistically significant( P < 0. 01),and there was no statistical significance in the difference between group B and the control group( P > 0. 05). [Conclusions]D. officinale Kimura et Migo from Longhushan can effectively reduce the fasting plasma glucose level and Hb A1 c of type 2 diabetic patients and effectively improve the symptoms of patients; clinical application of D. officinale Kimura et Migo has equivalent effect with metformin hydrochloride,and combination of D. officinale Kimura et Migo with metformin hydrochloride has better effect than single use.
文摘中药石斛基原复杂,其主流品种有霍山石斛Dendrobium huoshanense C.Z.Tang et S.J.Cheng、铁皮石斛D.officinale Kimura et Migo、金钗石斛D.nobile Lindl.,《中华人民共和国药典》(以下简称《中国药典》)2005年版石斛项下增加了“及其近似种”的描述,难以确定具体物种,有必要对其进一步正本清源。对石斛属的起源进行本草考证,对霍山石斛、铁皮石斛、金钗石斛的本草起源进行梳理,为不同石斛属药材的应用提供参考。霍山石斛是最早作为中药石斛应用的品种,高效液相色谱法特征图谱等方法为《中国药典》2020年版收录霍山石斛提供了技术支撑;铁皮石斛应为石斛药材中应用时间最久、范围最广的品种,其主要为浙江、云南、贵州,以及广东、广西、福建、江西等地丹霞地貌上生长的种群,应高度重视并对其进行保护;D.nobile在历代文献中的中文名称混乱,应明确将其作为金钗石斛的拉丁学名。枫斗是我国名贵中药和传统保健品,起源于霍山石斛。古代名医对霍山石斛、铁皮石斛、金钗石斛、川石斛的用法各有特色,建议厘清品种来源、功效,加强现代研究,在修订《中国药典》时将其各自单列,充分发挥中药石斛的临床价值。
文摘为探究12种必需矿质元素(包括N、P、K、Ca、Mg、S、Fe、Mn、Cu、Zn、B、Mo)对铁皮石斛(Dendrobium officinale Kimura et Migo)生长及生理指标的影响,以铁皮石斛3个月组培苗为材料,以MS培养基为基础设置全营养对照(CK)、单一缺素处理和缺素恢复处理,分析各处理条件下铁皮石斛的表型特征和生理变化。结果显示:缺N、P、Ca、Cu、Zn处理组铁皮石斛组培苗缺素症状明显,整株的生长状态及叶片和假鳞茎的解剖结构均受到影响;除Zn外,其他元素缺素恢复处理后症状均得到明显改善。缺N、K、Ca、S、Zn处理组假鳞茎长及缺N、Ca处理组的叶片数受抑制明显。除P外,其他元素缺素处理组叶片叶绿素含量明显降低,多数缺素恢复处理组叶片叶绿素a和叶绿素b含量较缺素处理组极显著(P<0.01)或显著(P<0.05)升高;缺Fe处理组的叶绿素a含量与叶绿素b含量的比值明显降低,恢复处理后接近于CK。除Fe外,其他微量元素缺素处理对叶片超氧化物歧化酶(SOD)活性抑制明显,而缺大量元素处理组的SOD活性均不同程度升高;缺P处理组的过氧化物酶(POD)活性明显降低,而缺N、Mg、S、Mn、Zn、Cu处理组的POD活性明显升高,除Mn外,其他元素缺素恢复处理组的POD活性均升高。缺N、P可大幅提高叶片可溶性糖含量,缺N、Mn、Cu可大幅提高叶片可溶性蛋白质含量。缺N、Cu处理组假鳞茎多糖含量大幅高于CK,Ca、Mo缺素恢复处理组假鳞茎多糖含量较缺素处理组极显著升高;缺Mg处理组假鳞茎总黄酮含量大幅高于CK。综上,在保证铁皮石斛正常生长的前提下,可通过Ca、Mo缺素恢复处理来提高铁皮石斛假鳞茎多糖含量,通过适量缺Mg处理来提高铁皮石斛假鳞茎总黄酮含量。
