期刊文献+
共找到10篇文章
< 1 >
每页显示 20 50 100
Kingfisher FLEX自动工作站在批量提取脱落细胞检材DNA中的应用 被引量:2
1
作者 袁家龙 袁红 《刑事技术》 2017年第1期83-84,共2页
目的建立基于自动工作站的大批量提取实验室日常检案受理的脱落细胞检材的DNA的方法。方法针对不同的脱落细胞检材分别采用直接剪取法、两步擦拭法、胶带粘取法或负压吸附法提取脱落细胞,应用Kingfisher FLEX自动工作站,结合使用LC-超... 目的建立基于自动工作站的大批量提取实验室日常检案受理的脱落细胞检材的DNA的方法。方法针对不同的脱落细胞检材分别采用直接剪取法、两步擦拭法、胶带粘取法或负压吸附法提取脱落细胞,应用Kingfisher FLEX自动工作站,结合使用LC-超微磁珠DNA提取试剂盒和Power Plex?18D试剂盒,对现场脱落细胞检材DNA进行批量提取和扩增,计算成功检出STR基因座的检材比率。结果应用该方法,成功检出STR基因座的检材比率为59.7%。结论该方法稳定、高效、检出率高,可用于脱落细胞检材DNA的批量提取。 展开更多
关键词 法医物证学 kingfisher FLEX 脱落细胞 DNA批量提取
下载PDF
Abundance and prey capture success of Common Terns(Sterna hirundo)and Pied Kingfishers(Ceryle rudis)in relation to water clarity in south-east coastal Ghana
2
作者 Lars H.Holbech Francis Gbogbo Timothy Khan Aikins 《Avian Research》 CSCD 2018年第3期226-238,共13页
Background: Water clarity may negatively influence rate of plunge diving and prey capture success of piscivorous plunge-diving birds, and therefore has implications for their conservation in polluted urban wetlands. W... Background: Water clarity may negatively influence rate of plunge diving and prey capture success of piscivorous plunge-diving birds, and therefore has implications for their conservation in polluted urban wetlands. We studied the relationship between water clarity and the abundance and prey capture success of Pied Kingfishers(Ceryle rudis) and Common Terns(Sterna hirundo) in two polluted coastal waters of south-east Ghana—the Weija Lake and Densu Delta Ramsar Site.Methods: On each wetland, data on abundance and prey capture success of plunge-divers were collected in four spatio-temporal quadrats of 100 m × 100 m and analysed with concurrent measurements of water quality parameters using GLM regression with Pearson's correlation coefficients.Results: Mean prey capture success of Pied Kingfishers(34.7 e two species responded differently to water clarity. The± 13.1%) and Common Terns(35.3 abundance of Common Terns was± 11.0%) were similar but th significantly higher in less transparent/more turbid water while that of Pied Kingfishers showed no significant relationship with turbidity and transparency. In contrast, the prey capture success of Common Terns was neither related to transparency nor turbidity, as opposed to that of Pied Kingfishers which was significantly higher in more turbid/less transparent waters. Correlations between capture success and bird abundance, as well as capture attempts were insignificant, suggesting that increased fish abundance associated with cloudy water may not necessarily promote higher abundance and capture success of foraging birds. Thus, when foraging in less transparent water, capture success may depend more on predator avoidance by fish prey than lower prey detectability of foraging birds.Conclusion: Within a gradient of 15–51 cm transparency studied, lower water clarity did not constrain prey capture success of Common Terns and Pied Kingfishers. Further studies on the foraging ecology of plunge-divers in coastal Ghana are however required to make firm conclusions on the relationship between water clarity and foraging birds and fish prey abundances, as well as capture success. 展开更多
关键词 Coastal Ghana Common Tern PIED kingfisher PREY capture SUCCESS Water TURBIDITY and transparency
下载PDF
KingFisher Flex核酸纯化系统上树木叶片DNA的提取方法 被引量:3
3
作者 杨合宇 李发根 +5 位作者 翁启杰 李梅 陈升侃 姬红霞 周长品 甘四明 《分子植物育种》 CAS CSCD 北大核心 2017年第5期1854-1861,共8页
以桉树4种/杂种的叶片为材料,比较了5种磁珠试剂盒在King Fisher Flex核酸纯化系统上自动提取的DNA得率和纯度,初步选出了2种较好的试剂盒。比较了King Fisher Flex上不同的洗涤速度,最优为Medium。初选的2种试剂盒所提的DNA均可有效用... 以桉树4种/杂种的叶片为材料,比较了5种磁珠试剂盒在King Fisher Flex核酸纯化系统上自动提取的DNA得率和纯度,初步选出了2种较好的试剂盒。比较了King Fisher Flex上不同的洗涤速度,最优为Medium。初选的2种试剂盒所提的DNA均可有效用于PCR扩增,表明DNA纯度均较好,确定得率最高的为最优试剂盒。对比手动的改良CTAB法(DNA得率19.0μg/g,OD_(260)/OD_(280)为1.97,OD_(260)/OD_(230)为2.03),本研究优化的方法可显著提高DNA得率(103.6μg/g)且DNA纯度相似(OD_(260)/OD_(280)为1.93,OD_(260)/OD_(230)为1.89)。优化的方法可有效用于马占相思、银中杨、降香黄檀、思茅松、马尾松和短枝木麻黄叶片的DNA提取。这对树木分子生物学研究的高通量DNA提取有重要的应用价值,对非木本植物的DNA提取和其他基于磁珠法的纯化系统亦可提供有益的方法参考。 展开更多
关键词 树木 DNA提取 kingfisher FLEX
原文传递
基于Kingfisher Flex系统树木叶片RNA的提取方法比较及优化 被引量:3
4
作者 何佳悦 王莉 +6 位作者 贾翠蓉 朱显亮 王亚琴 翁启杰 周长品 甘四明 李发根 《分子植物育种》 CAS CSCD 北大核心 2020年第9期3009-3017,共9页
随着林木功能基因组学的发展,如何快速高效地提取高质量的核酸变得越来越重要。本研究基于Kingfisher Flex全自动磁珠提取纯化系统,以细叶桉叶片为材料,利用5种磁珠法RNA提取试剂盒(A/B/C/D/E)比较提取RNA的质量和纯度,筛选出试剂盒C为... 随着林木功能基因组学的发展,如何快速高效地提取高质量的核酸变得越来越重要。本研究基于Kingfisher Flex全自动磁珠提取纯化系统,以细叶桉叶片为材料,利用5种磁珠法RNA提取试剂盒(A/B/C/D/E)比较提取RNA的质量和纯度,筛选出试剂盒C为最佳试剂盒。在试剂盒C默认提取程序基础上,利用正交设计L9(34),研究Kingfisher Flex全自动磁珠提取纯化系统上不同洗涤速度、洗脱速度及时间等机器参数对提取RNA的浓度和纯度的影响。正交试验结果表明,对提取RNA的浓度和纯度影响最大的机器参数是洗涤速度,正交试验最优程序组合下提取RNA的纯度稍优于试剂盒C,但是得率仅为试剂盒C的1/3,综合比较后确认试剂盒C默认程序为提取细叶桉叶片RNA的最优提取程序。另外利用试剂盒C也分别对降香黄檀、大果紫檀、油楠、檀香、黑木相思和柚木这六个树种的叶片RNA进行提取,结果表明提取出的RNA均满足cDNA建库以及转录组测序实验的要求。本研究建立了Kingfisher Flex全自动磁珠提取纯化系统上树木叶片RNA的高效提取方法,对树木叶片高通量RNA提取有重要的应用价值,并为其他树种在提取RNA程序上对相关参数进行优化和设置提供了参考。 展开更多
关键词 kingfisher Flex系统 树木叶片 RNA提取 磁珠法RNA提取试剂盒
原文传递
Effect of Feather Elasticity of Kingfisher Wing on Droplet Impact Dynamics 被引量:3
5
作者 Chengchun Zhang Zhengyang Wu +2 位作者 Xiumei Zhang Yongli Yue Jing Wang 《Journal of Bionic Engineering》 SCIE EI CSCD 2018年第4期731-740,共10页
We experimentally studied droplet impact dynamics onto wing feathers of kingfishers. Distilled water droplets with a fixed diameter of 2.06 mm were used as drop liquid and the initial impact velocities of droplets var... We experimentally studied droplet impact dynamics onto wing feathers of kingfishers. Distilled water droplets with a fixed diameter of 2.06 mm were used as drop liquid and the initial impact velocities of droplets varied from 0.28 m· s^-1 to 1.60 m·s^-1. Two high-speed cameras were utilized to capture the impact process of water droplets onto the wing feather surface from both horizontal and vertical directions. Two states of the feathers (elastic and inelastic) were considered to study the influence of elasticity. At the entire impact ve- locity range we studied, regular rebound, bubble trapping and jetting, partial pinning and partial rebound of droplets on inelastic wing feather surface were observed as the initial impact velocity increased. However, only one dynamic behavior (regular rebound) was found on the elastic wing feather surface. The elasticity plays a more important role in the direction difference of droplet spreading than wing feather microstructure. The contact time of water droplets on the elastic wing feather surface was less than that on the inelastic surface within the range of Web numbers from 1.06 to 36 under test conditions. 展开更多
关键词 BIONIC droplet impact kingfisher feather ELASTICITY contact time spreading behavior
原文传递
KF油田模糊带成因分析及技术对策
6
作者 张鹏 陈磅 《内蒙古石油化工》 CAS 2023年第11期57-61,共5页
Kingfisher油田位于乌干达Albert湖区东南岸,Albert湖区为克拉通之上的大型裂谷盆地。受近岸东西向物源快速沉积等地质因素、采集参数、资料处理等因素影响,油田主体区成像差,地震资料在上新统目的层,存在明显模糊区,地层产状难以刻画,... Kingfisher油田位于乌干达Albert湖区东南岸,Albert湖区为克拉通之上的大型裂谷盆地。受近岸东西向物源快速沉积等地质因素、采集参数、资料处理等因素影响,油田主体区成像差,地震资料在上新统目的层,存在明显模糊区,地层产状难以刻画,模糊区成像问题制约了中浅部目的层储层预测及表征。为解决模糊带成像问题,针对KF油田,结合地震地质条件,系统开展了模糊带地震波场传播机理分析,模糊带成像主要由于因为高部位自冲沟而下的快速现代沉积,造成边界断层附近浅部地层欠压实,地层速度偏低,这是影响模糊带成因最主要因素。现有资料采集参数主要以勘探评价为主要目的,气枪震源穿透力弱,不利于成像;覆盖次数低,不利于提高信噪比;纵横向采样密度不够,不利于断层成像和油藏描述,难以满足油气田开发需求,是影响模糊带成因次要因素。厘清了影响地震资料品质的因素,提出了改善成像采集和处理对策,为KF油田下一步开发提供了指导依据。 展开更多
关键词 kingfisher油田 模糊带成像 浅层气屏蔽 宽频震源 宽频处理第一
下载PDF
微量脱落细胞DNA提取纯化方法比较 被引量:2
7
作者 江煜灵 巫家盛 +1 位作者 赵春鹤 袁家龙 《黑龙江科技信息》 2016年第9期34-35,共2页
本文比较了实验室常用的kingfisher flex工作站纯化方案及kingfisher FL纯化方案的检验效果。日常案件检材的检验结果表明运用kingfisher flex方案对微量脱落细胞DNA进行纯化的检测结果优于kingfisher FL方案。实验检材检验结果也提示... 本文比较了实验室常用的kingfisher flex工作站纯化方案及kingfisher FL纯化方案的检验效果。日常案件检材的检验结果表明运用kingfisher flex方案对微量脱落细胞DNA进行纯化的检测结果优于kingfisher FL方案。实验检材检验结果也提示在微量范围内,kingfisher flex方案优于kingfisher FL方案,在检材DNA含量较多时,两种纯化方案效果相当。 展开更多
关键词 kingfisher FLEX kingfisher FL 脱落细胞 微量 DNA纯化
下载PDF
沃特公司向美国海军推销无人海上飞机
8
作者 肖霞 《电子对抗》 2005年第4期35-35,共1页
沃特飞机公司透露了一种武装无人海上飞机概念,沃特公司说,这个概念在当今无人机领域内是独特的,其绰号是“鱼狗”Ⅱ(Kingfisher Ⅱ)。
关键词 无人海上飞机 kingfisher 传感器 沃特公司 美国 海军
下载PDF
Dietary selenium requirement of yellowtail kingfish (<i>Seriola lalandi</i>) 被引量:9
9
作者 Ky Trung Le Ravi Fotedar 《Agricultural Sciences》 2013年第6期68-75,共8页
The dietary selenium (Se) requirement of yellowtail kingfish (Seriola lalandi) in normal and infected conditions was investigated. The fish were fed one of five experimental diets;a control un-supplemented diet (3.35 ... The dietary selenium (Se) requirement of yellowtail kingfish (Seriola lalandi) in normal and infected conditions was investigated. The fish were fed one of five experimental diets;a control un-supplemented diet (3.35 mg/kg Se) or diets supplemented with Se to provide 4.86, 5.38, 5.85 or 6.38 mg/kg Se. After feeding for 6 weeks, the fish were challenged by Vibrio anguillarum immersion and then observed for 4 weeks. Supplementation of Se had no effect on feed intake, feed conversion ratio and survival over 6 weeks of feeding;however, it significantly increased growth and Se content in muscle tissues. The optimal Se level for maximal growth of yellowtail kingfish estimated by second order regression was 5.56 mg/kg. Following the bacterial challenge, the immune-stimulating effects of Se were demonstrated in lysozyme and bactericidal activities, and there was a corresponding increase in survival and antibody response by supplementation of Se at ≥2 mg/kg (measured Se of ≥5.38 mg/kg). Under normal and infectious conditions, antioxidant capacity of fish measured as glutathione peroxidase activity increased by supplementation of Se. During post-challenge period, haematocrits were higher in the fish fed Se supplemented diets than the fish fed the control diet, while more macrophage aggregates were seen in the control group than in the others. Furthermore, there was evidence of myopathy in fish fed the diet without Se supplementation. Therefore, the results indicated that the optimal dietary Se requirement of yellowtail kingfish is 5.56 mg/kg. 展开更多
关键词 SELENIUM REQUIREMENT Vibrio ANGUILLARUM YELLOWTAIL Kingfish
下载PDF
Stakeholders’ Views on Management Arrangements: A Case of Kingfish Fishery in the Sultanate of Oman 被引量:1
10
作者 Abdullah Hilal Al-Balushi Shekar Bose Anesh Govender 《Natural Resources》 2016年第5期251-264,共14页
This paper exemplifies a primary step towards eliciting primary and secondary stakeholders’ views on management issues pertaining to kingfish fishery in Oman, and potential options for effective management of the fis... This paper exemplifies a primary step towards eliciting primary and secondary stakeholders’ views on management issues pertaining to kingfish fishery in Oman, and potential options for effective management of the fishery using questionnaire surveys and focus group interviews. There was consensus from stakeholder groups which included fishers, fishery managers, and fishery scientists that the current stock condition is not biologically sustainable. It is found that fishing effort control and technical measures are preferred to catch control by both groups. The role of mass media and the traditional institution in communicating fisheries issues are found to be relatively minor. Although the overall rating on the comprehensiveness of the proposed plan is promising, there are significant differences between the two groups with regard to legislative arrangements (χ<sup>2</sup> = 24.793, p-value = 0.000), management goals (χ<sup>2</sup> = 16.206, p-value = 0.001), operational objectives (χ<sup>2</sup> = 19.884, p-value = 0.000), performance indicators (χ<sup>2</sup> = 15.524, p-value = 0.001), and measures (χ<sup>2</sup> = 13.483, p-value = 0.004). Policy implications of the key findings are discussed in both national and regional contexts. Management authorities can use these findings to design an appropriate plan of actions for achieving sustainability in this fishery. 展开更多
关键词 Sustainability Kingfish Fishery Stakeholder Views Management Arrangements Oman
下载PDF
上一页 1 下一页 到第
使用帮助 返回顶部