Java CoG Kits在GridFTP开发中的应用

通过对Java CoG Kits软件包的研究,介绍了Java CoG Kits的结构和GridFTP的特性,讨论了Java CoG Kits中用于开发GridFTP客户端的FTP包的组件层次和类库,描述了GridFTP客户端类库用于编写GridFTP应用的函数,研究了GridFTP第三方控制数据传输模型,给出了实现的过程和步骤,最后基于Java CoG Kits设计实现了GridFTP第三方控制数据传输。

Comparison of Sputum Smear Microscopy and Rapid Tuberculosisantibody Detection Test Kits for Diagnosis of Pulmonary Tuberculosis in Abia State, Nigeria

The SSM （sputum smear microscopy） and five immunochromatographic tuberculosis antibody detection tests （DiaSpot TB, Spodex TB, SD Rapid TB, Clinotech TB Screen and Precious One-step TB） were compared for diagnosis of active TB at the Leprosy and Tuberculosis Referral Hospital, Uzuakoli, Abia State, Nigeria. Sputum specimens from 150 study participants （male/female ratio, 0.81） were cultured on Lowenstein-Jensen slopes and direct smears were stained by Ziehl-Neelsen technique and examined by light microscopy. Sera were tested for anti-TB antibodies using the rapid TB tests. A total of 91 participants were culture positive, 79 （86.8%） for M. tuberculosis and 12 （13.2%） for nontuberculous mycobacteria. The sensitivity of SSM was 50% （95% CI： 39.0-61.0） and specificity was 92.3% （95% CI： 86.4-98.2） in those culture positive for M. tuberculosis. The sensitivity and specificity of the Rapid TB tests ranged from 24.1-39.2% and 78.4-87.8%, respectively. None of the five rapid TB tests had acceptable level of accuracy for diagnosis of active TB. The sensitivity of SSM though moderate is inadequate for long term TB control in this setting.

Comparative Study on 4 EIA Kits for Screening Antibody to Hepatitis C Virus in Pooled Sera

Four enzyme immunoassay (EIA) test kits, 1 Canadian product and 3 Chinese products,were used in the comparative study. Each pool consisted of 5 sera, and the 5 single sera were tested as controls. The tests were carried out according to the instructions, keeping the same dilution of each serum in single and pool samples. It was found that with the Canadian kit,the positive and negative results of opled sera had no difference from that of the controls (P>0. 10). In the case of Chinese Yali and Kehua kits, the positive results of pooled sera showed no difference from the controls (P >0. 10), but the optical density (OD) of negative opls were increased (P < 0. 01 ), though quite distant from the cut-off values. In the case of Changzheng kit, the OD of opitive opls were significantly lower than those of the controls (P < 0. 05 ), and weak positive samples missed the detection. However this problem could be overcome by blocking the microwells beforehand. Our experiment demonstrate that not all EIA test kits are suitable for screening opls for antithey to hepatitis C virus, and that it is important to assess the sensitivity of the EIA kit to be used for this purpose.

Consistency Analysis of Detection Results of Two Herpes Simplex Virus (HSV) Type II Nucleic Acid Detection Kits

Objective: The objective of the study is to verify the clinical validity of the following kits with the comparative experimental analysis and evaluate whether their performance can meet the clinical requirements, i.e. Class III in vitro diagnostic reagent “Herpes Simplex Virus (HSV) Type II Nucleic Acid Detection Kit (PCR-Fluorescence Probe Method)” of Daan Gene Co., Ltd. (Daan kit for short) and “Herpes Simplex Virus (HSV) Type II Nucleic Acid Detection Kit (Fluorescence PCR Method)” of Wuhan Biot Gene Co., Ltd. (Biot kit for short). Method: In the study process, the samples were divided into positive and negative groups according to the control test results, and the clinical application performance of Daan kit and Biot kit was evaluated by comparing their test results. Results: The results show that two kits indicate the same test results, i.e. 26 positive and 107 negative samples in a total of 133 male urethral discharge samples, and 32 positive and 238 negative samples in a total of 270 female cervical secretion samples. Conclusion: It can be concluded from the clinical test that Daan and Biot Herpes Simplex Virus (HSV) Type II Nuc- leic Acid Test Kits are reliable, accurate, safe, convenient for use, stable and high-value in the clinical application.

DNA extraction from paraffin embedded colorectal carcinoma samples: A comparison study of manual vs automated methods,using four commercially kits

BACKGROUND Nucleic acid isolation from formalin-fixed, paraffin-embedded tissue(FFPET)samples is a daily routine in molecular pathology laboratories, but extraction from FFPET is not always easily achieved. Choosing the right extraction technique is key for further examinations.AIM To compare the performance of four commercially available kits used for DNA extraction in routine practice.METHODS DNA isolation was performed on 46 randomly selected formalin-fixed, paraffinembedded(FFPE) colorectal adenocarcinoma(CRC) surgical specimens. Four commercially available extraction kits were used: two for manual DNA extraction(the Pure Link Genomic DNA Mini Kit from Invitrogen and the High Pure FFPE DNA Isolation Kit from Roche) and two for automated DNA extraction(the i Prep Genomic DNA Kit from Invitrogen and the Magna Pure LC DNA Isolation Kit from Roche). The DNA concentration and quality(odds ratio) among the four systems were compared. The results were correlated with the clinicopathological aspects of CRC cases: age, gender, localization, macro-and microscopic features,lymph node metastases, and the lymph node ratio.RESULTS The highest DNA concentration was obtained using the manual kits: 157.24 ±62.99 ng/μL for the Pure Link Genomic DNA Mini Kit and 86.64 ng/μL± 43.84 for the High Pure FFPE DNA Isolation Kit(P < 0.0001). Lower concentrations were obtained with automated systems: 20.39 ± 21.19 ng/μL for the Magna Pure LC DNA Isolation Kit and 8.722 ± 6.408 ng/μL for the i Prep Genomic DNA Kit,with differences between the systems used(P < 0.0001). The comparison between age, gender, tumor localization, pT or pN stage and the lymph node ratio indicated no statistically significant difference in DNA concentration using any of the nucleic acid isolation kits. DNA concentration was influenced by the macroscopic features and grade of differentiation. A higher DNA concentration was obtained for well-differentiated polypoid colorectal adenocarcinomas(CRCs), compared with undifferentiated ulcero-infiltrative carcinomas,irrespective of the kit used.CONCLUSION For research or diagnosis that needs high DNA concentrations, manual methods of DNA isolation should be used. A higher amount of DNA can be obtained from polypoid-type differentiated CRCs. Automated systems confer comfort and a lower amount of DNA that is, however, sufficient for classic polymerase chain reaction(PCR) and real-time quantitative PCR molecular examinations. All four commercially available kits can be successfully used in daily practice.

Studies on Rapid Quantitative Analysis of Bt Toxin by Using Envirologix Kits in Transgenic Rice

Investigations were done on the usefulness of Envirologix CrylAb/CrylAc Plate kits for quantitative analysis of Bt toxin content in transgenic rice grains. Two transgenic rice lines: Kemingdao 1 (KMD1) and Kemingdao 2 (KMD2), transformed with a crylAb gene, and their parental variety, cv. Xiushui 11, were used as positive and negative samples. Results showed that the correlation coefficients as high as 0. 985 - 0. 998, significant at probability level of 0. 05 or 0.01, were obtained for linear regression equations by using the appended calibrators of the kits. No significant differences were detected for values of same rice sample obtained from different trials or by using different lots of the product (kit). The detectable Bt toxin content by this method could be as low as 0.5 ng/g. The Envirologix Kit could be useful for rapid quantitative detection of Bt toxin in rice grains because of its preciseness, simplicity and time saving.

Sensitivity and specificity of five malaria rapid diagnostic kits used in south-south region of Nigeria

Sensitivity and specificity of five commonly used rapid malaria test kits in South-South geopolitical zone of Nigeria were analyzed, namely Plasmotest (Biotech International, Germany), Malariatest (Acon Diagnostic, USA), AcumPF (Acumen, China), PF test (Diaspot, Indonesia) and Malaria P.f. (Global, USA). A total of 200 positive malaria blood sample using microscopy as gold standard and 200 negative were obtained from adult and children. All the kits were tested against these gold standards as directed by the manufacturers. Of the 100 positive gold standard used from children sample, 96% sensitivity were recorded for Biotech, Acon and Global 97%, while Acumen and Diaspot had 99% and 98% respectively. The the highest Specificity was recorded with Biotech 99% both Acumen and Diaspot had 97% specificity. In adult blood sample, there was general reduction in the sensitivity of the test kits. We obtained Sensitivity of 30% with Biotech, 46% with Acon, Global 73%, Diaspot 75%, and Acumen 78%. Considering the increasing influx of MRDT’s into the country, and from the result obtained from this study, there is need for control agensis to ensure that diagnostic test kits be reevaluated before introduction into Nigeria market.

Solving Electricity Deficit in Kinshasa With Solar Kits

This paper highlights the importance of adopting use of solar kits to overcome the shortage of electricity supply in the city of Kinshasa instead of polluting generators.Given a need for electricity delivery estimated at 5 kWh for a poor household of three occupants,a 1 kW solar kit sold for US$990 would perfectly replace SNEL network,the unique electric utility company that uses rolling blackout to handle its low power supply capability within the town,with all the environmental benefits in lieu of a 4 kW power generator sold at US$900 with all the consequences related to the use of fossil fuels in terms of CO2 emissions harmful to health and the environment.Similarly for a middle class household of five occupants,a solar kit of 3 kW to 2,700 dollars or 5 kW to 4,600 dollars is far preferable to an 8 kW generator of US$4,155 which pollutes and entails expenses related to the fuel consumption.

基于转录测序技术对KIT基因在听觉色素障碍类疾病初步研究

目的 利用KITF860S突变耳聋小鼠模型研究探讨KIT基因突变导致听觉色素障碍类疾病致聋的分子机制。方法 利用稳定遗传的KITF860S小鼠模型家系,并对家系中不同表型个体进行长期听觉电生理测试、同窝不同表型小鼠病理切片、进行全基因组转录测序分析其表达差异基因。结果 该小鼠模型家系内杂合型小鼠在初期与野生型小鼠听力表型并无明显差距,在5月龄时表现为渐进性听力下降,纯合型小鼠在听力形成初期即表现为先天性中重度听力下降,10周龄时即表现为全聋表现。转录测序结果显示,以野生型小鼠为对照,氧化磷酸化通路在KIT基因纯合突变及杂合突变中均表现为下调,紧密连接通路在KIT基因不同组别比较中为不同上下调表现。结论本研究结果表明,KIT基因突变所致听觉色素障碍类疾病表现与氧化磷酸化、紧密连接等通路相关目的基因靶蛋白表达异常有一定相关性,最终导致听力损失。对KIT基因突变致病分子机制的研究对于丰富人类耳聋基因库具有重要意义,也为后续相关综合征类疾病基因治疗提供理论基础。

难治性晚期KIT外显子9突变胃肠间质瘤患者的治疗探索:靶向联合治疗加肿瘤减灭术

KIT外显子9突变是胃肠间质瘤第二常见的基因突变类型,较其他类型呈现恶性度更高的生物学行为。大多数KIT外显子9突变胃肠间质瘤经标准序贯治疗后并不能获得满意的无进展生存期。本文报道了1例44岁晚期KIT外显子9突变女性患者,在标准二线治疗进展后予阿伐替尼联合舒尼替尼治疗,1个月后肿瘤退缩达15.7%,获得手术机会。患者术后继续靶向联合治疗,截至2023年7月,共有6个月未见肿瘤复发。

Summary of the Detection Kits for SARS-CoV-2 Approved by the National Medical Products Administration of China and Their Application for Diagnosis of COVID-19

The on-going global pandemic of coronavirus disease 2019(COVID-19)caused by a novel coronavirus called severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)has been underway for about 11 months.Through November 20,2020,51 detection kits for SARS-CoV-2 nucleic acids(24 kits),antibodies(25 kits),or antigens(2 kits)have been approved by the National Medical Products Administration of China(NMPA).Convenient and reliable SARS-CoV-2 detection assays are urgently needed worldwide for strategic control of the pandemic.In this review,the detection kits approved in China are summarised and the three types of tests,namely nucleic acid,serological and antigen detection,which are available for the detection of COVID-19 are discussed in detail.The development of novel detection kits will lay the foundation for the control and prevention of the COVID-19 pandemic globally.

LSTM RNN:detecting exploit kits using redirection chain sequences

While consumers use the web to perform routine activities,they are under the constant threat of attack from malicious websites.Even when visiting‘trusted’sites,there is always a risk that site is compromised,and,hosting a malicious script.In this scenario,the injected script would typically force the victim’s browser to undergo a series of redirects before reaching an attacker-controlled domain,which,delivers the actual malware.Although these malicious redirection chains aim to frustrate detection and analysis efforts,they could be used to help identify web-based attacks.Building upon previous work,this paper presents the first known application of a Long Short-Term Memory(LSTM)network to detect Exploit Kit(EK)traffic,utilising the structure of HTTP redirects.Samples are processed as sequences,where each timestep represents a redirect and contains a unique combination of 48 features.The experiment is conducted using a ground-truth dataset of 1279 EK and 5910 benign redirection chains.Hyper-parameters are tuned via K-fold cross-validation(5f-CV),with the optimal configuration achieving an F1 score of 0.9878 against the unseen test set.Furthermore,we compare the results of isolated feature categories to assess their importance.

胃肠间质瘤的分子靶向药物研究与应用进展

胃肠道间质瘤(GIST)是消化道最常见的间充质肿瘤,占所有胃肠道肿瘤的1-2%,约有80%和10%的GIST分别存在的KIT和PDGFRA突变,还有约10%的GIST不存在KIT和PDGFRA突变,称之为野生型GIST。手术切除仍是治疗GIST的首选方法,但对于复发、转移或不可切除的GIST,靶向药物酪氨酸激酶受体抑制剂(TKI)伊马替尼是治疗GIST的首选药物,由于GIST基因突变位点的不同,对不同的靶向药物的敏感度也不同,因此需要不同的药物治疗,并且随着治疗的进行,越来越多的患者会出现继发耐药性,传统的靶向药物已经难以为继,因此需要更换新的靶向药物来治疗,如舒尼替尼、瑞戈非尼、瑞普替尼等药物,因此本文对GIST的靶向药物的研究现状进行综述。

Sensitivity of Commercial Enzyme Inhibition Colorimetric Pesticide Residue Rapid Test Kit to a Variety of Pesticides

[Objectives]To fully understand the quality of commercial enzyme inhibition-colorimetric pesticide residue rapid detection kits,so that they can play a greater role in the detection and supervision of agricultural products.[Methods]The sensitivity of 28 kinds of pesticides was determined by using the commercially available enzyme inhibition colorimetric rapid detection kit with Hendu brand.[Results]There was a significant difference in the sensitivity of the kit to each pesticide,and the kit was more sensitive to dichlorvos among the 28 pesticides tested.The sensitivity to methyl isosalifos,dimethoate,isocarbophos,fenthion and phorate was poor,and the sensitivity to quinalphos was different between 3.0 and 2.5 mL.[Conclusions]The large difference of the sensitivity of the enzyme inhibition-colorimetric rapid detection kit for pesticide residues to different kits is a reason for the false positive and false negative test results of the kit,which needs to be considered by relevant personnel.

基于Raspberry Pi及Drone Kit的无人机飞行控制教学实践应用

基于开源硬件Raspberry Pi,Pixhawk及开源软件Drone Kit,设计了高校无人机飞行控制教学实践课程。课程中使用Raspberry Pi作为无人机的机载计算机与Pixhawk飞控芯片协同工作,通过使用MAVLink协议通信。学生在实践操作中需要掌握硬件各接口的功能属性进行无人机组装,在调测环境下,基于Drone Kit开发无人机飞行控制程序。所提的实践课程可以提高学生的动手实践能力,新环境下的创新应用能力能加深对飞行控制技术的理论理解。

A Clinical Study to Assess the Effectiveness of Oral Combination Kit Therapy in Syndromic Management of Abnormal Vaginal Discharge (FEMINE Study) in Kinshasa, Democratic Republic of Congo

Background: Vaginal discharge is one of most common and nagging problems that women face. About 20% - 25% of women who visit gynecology department complain of vaginal discharge and leucorrhoea. An orally administered combination kit, containing 2 g secnidazole, 1 g azithromycin and 150 mg fluconazole (Azimyn FS Kit), has been successfully evaluated in clinical trials and used in several countries for management syndromic vaginal discharge due to infections. Methods: This is a longitudinal study which aimed to verify the clinical efficacy of the combined oral kit containing secnidazole, azithromycin and fluconazole (Azimyn FS Kit®) in the syndromic treatment of abnormal vaginal discharge in patients received in outpatient consultations in Kinshasa/DR Congo from March to September 2023. Results: Majority of patients had whitish vaginal discharge (51.6%) of average abundance (56.2%), accompanied by pruritus in 72.1% of cases, and dyspareunia in 23.5% of cases and hypogastralgia in 40.2% of cases. One week after treatment with the Azimyn FS® combined kit, at the greatest majority of patients (97.3%), abnormal vaginal discharge had decreased by more than 50% (84.1%). Two weeks after treatment with the Azimyn FS® combined kit, almost all patients (97.3%) no longer had abnormal vaginal discharge which had completely disappeared. Conclusion: A single dose of secnidazole, azithromycin and fluconazole in the form of an oral combi-kit (Azimyn FS Kit) has shown excellent therapeutic effectiveness in the syndromic treatment of abnormal vaginal discharge wherein patients were treated without diagnostic confirmation.

雪山的回响 Kitoron之森温泉度假酒店

Kitoron之森温泉度假酒店位于日本北海道东川町的一处草地之上,与隈研吾事务所北海道办公室相去不远,从这个山坡可以俯瞰整个温泉小镇,也是北海道地区最著名的景点之一。整栋建筑的外墙材料取自北海道当地的落叶松,而室内部分和家具则主要采用当地的桦木,并由设计师与来自东川町的木匠共同设计并制作完成。

Y‑STR Kits and Y‑STR Diversity in the South African Population: A Review

The South African population consists of four ethnic groups,i.e.,Blacks,Coloreds,Indians,and Whites,and is considered the most diverse conglomeration of humans.In addition to autosomal short tandem repeat(STR)variation,an important tool to study population diversity is Y-chromosome(Y)-STR analysis.Y-STRs aid in forensic investigations and provide essential data about paternal lineage origins.Y-STR kits consisting of an array of stable and rapidly mutating markers offer crucial information on a given population’s genetic and haplotype diversity.This review discusses the development of Y-STR kits over the years and highlights some prominent Y-STR studies conducted on the South African population.The earliest Y-STR kit developed was the Y-PLEX™6,with the most recent being the UniQTyper™Y-10 Multiplex.The South African population studies show varying data,with the“minimal haplotype”having low discrimination capacity among the ethnic groups and the UniQTyper™Y-10 showing high genetic diversity among the ethnic groups of the country.There is a dearth of Y-STR studies on the South African population.With the advent of new Y-STR kits with increased discriminatory markers,additional studies are required to represent the South African population in the Y-STR databases.Considering the diversity of the South African population,establishment of a local/regional population database would be beneficial.In addition,data on the origins and prevalence of mutations and silent alleles should be obtained from STR datasets generated during kinship investigations(specifically,parentage tests)so that detailed information about the frequencies of mutations,silent alleles,and uniparental disomy in the South African population at Y STR loci can be estimated.

Self-Testing Medical Kits Not Far-Fetched-Doctor

站在新世纪的起跑线上,遥望新科技的前景,我们感到绵延了数千年的大同小异的人类生活将发生根本性的巨变。许多传统的、想来无法更改的生活方式,或许将面目一新。 本文的主题句不同凡响,它不是“急吼吼”地去囊括文章的中心思想,而是使用轻松活泼的语言,轻点主题: Do you dread going to the doctor? In the 2Ist century.you may not have to-self-diagnosis(自我诊断)couldbe as easy as swallowing(吞下)a little pill. 得了病,吞服一粒药丸,诊断随即获得。诊断的结果再被传送到医生那里,而对症下药的处方随即又从医生那儿传送过来。问题就是这么简单！ 本文称这种化验体检的方法为 near-patient tests/on-the-spot checks,其后的同位语对它解释道:testing and diagnosing patients on the spot without theneed to send samples to a laboratory。据此,我们是否可以将它译成:就地检验。 关于此项创造的原动力,文章有所提及: Catering for(迎合)astronauts’medical needs on space missions and themilitary’s need to detect agents(试剂)of biological warfare had provided themain impetus(动力)for the development of the kits, he said. 其中的the military’s need让人感觉不是滋味。人类,