Kiwifruit(Actinidia deliciosa)‘Bruno’is prone to accumulate ethanol rapidly after respiratory climacteric during storage at ambient conditions without stresses,which causes quality deterioration of the fruit associa...Kiwifruit(Actinidia deliciosa)‘Bruno’is prone to accumulate ethanol rapidly after respiratory climacteric during storage at ambient conditions without stresses,which causes quality deterioration of the fruit associated with alcohol off-flavor.For maintaining the postharvest quality of kiwifruit‘Bruno’,the effects of 1.0μL•L^−11-methylcyclopropene(1-MCP)treatment on regulating the ethanol fermentation andγ-aminobutyric acid(GABA)shunt pathway associated with control of alcohol off-flavor were investigated during storage at room temperature(24±1)°C for 27 days.The results showed that 1-MCP treatment significantly reduced the respiration rate,ethylene production,decay rate,ascorbic acid(AsA)loss,and delayed the decline in the firmness and titratable acidity(TA),and the increase in total soluble solid(TSS)in kiwifruit.Furthermore,1-MCP treatment effectively inhibited the increases in contents of acetaldehyde,ethanol,and GABA along with the suppressed activities of key enzymes involved in ethanol fermentation and GABA shunt pathway,such as pyruvate decarboxylase(PDC),alcohol dehydrogenase(ADH),glutamate decarboxylase(GAD),and GABA-transaminase(GABA-T)in kiwifruit during storage.In conclusion,1-MCP treatment efficiently regulated the ethanol fermentation and GABA shunt pathway by delaying the ripening process to avoid the alcohol off-flavor development,thereby contributed to maintaining the quality of the kiwifruit.展开更多
Kiwifruit is an important fruit crop that is highly sensitive to environmental stresses,such as drought,heat,cold,water logging and phytopathogens.Therefore it is indispensable to identify stress-responsive candidate ...Kiwifruit is an important fruit crop that is highly sensitive to environmental stresses,such as drought,heat,cold,water logging and phytopathogens.Therefore it is indispensable to identify stress-responsive candidate genes in kiwifruit cultivars for the stress resistance improvement.Here we report the isolation and characterization of a novel kiwifruit R1R2R3-MYB homolog(AcMYB3R)whose expression was induced by drought,salinity and cold stress.In vitro assays showed that AcMYB3R is a nuclear protein with transcriptional activation activity by binding to the cis-element of the kiwifruit orthologue of G2/M phase-specific gene KNOLLE.The Arabidopsis transgenic plants overexpressing AcMYB3R showed drastically enhanced tolerance to drought and salt stress.The expressions of stress-responsive genes such as RD29A,RD29B,COR15A and RD22 were prominently up-regulated by ectopic expression of Ac MYB3R.Our study provides a valuable piece of information for functional genomics studies of kiwifruit and molecular breeding in improving stress tolerance for crop production.展开更多
以秦美猕猴桃（Actinidia deliciosa C.F．Liang et A．R．Ferguson．Vat．Qinmei）果实为材料，研究了1-甲基环丙烯（1-MCP）处理在低温下对猕猴桃果实生理和品质的影响。0～2℃冷库贮存时，1-MCP能延缓猕猴桃硬度降低、重量损失、果...以秦美猕猴桃（Actinidia deliciosa C.F．Liang et A．R．Ferguson．Vat．Qinmei）果实为材料，研究了1-甲基环丙烯（1-MCP）处理在低温下对猕猴桃果实生理和品质的影响。0～2℃冷库贮存时，1-MCP能延缓猕猴桃硬度降低、重量损失、果实中维生素C含量的下降，抑制猕猴桃果实的可溶性固形物含量上升，保持较高的好果率，有显著的保鲜效果。低温下1-MCP明显抑制猕猴桃果实的呼吸作用，降低呼吸强度和推迟呼吸高峰出现，同时延缓乙烯释放高峰出现的时间。1-MCP保鲜的机理可能存在对乙烯受体抑制和乙烯合成抑制两种机制。展开更多
基金The research was supported by Natural Science Foundation of Zhejiang Province for youth(Grant No.LQ19C200004)the National Natural Science Foundation(Grant No.31671908)the Science and Technology Planning Project of Linhai City(Grant No.2018SHJH04).
文摘Kiwifruit(Actinidia deliciosa)‘Bruno’is prone to accumulate ethanol rapidly after respiratory climacteric during storage at ambient conditions without stresses,which causes quality deterioration of the fruit associated with alcohol off-flavor.For maintaining the postharvest quality of kiwifruit‘Bruno’,the effects of 1.0μL•L^−11-methylcyclopropene(1-MCP)treatment on regulating the ethanol fermentation andγ-aminobutyric acid(GABA)shunt pathway associated with control of alcohol off-flavor were investigated during storage at room temperature(24±1)°C for 27 days.The results showed that 1-MCP treatment significantly reduced the respiration rate,ethylene production,decay rate,ascorbic acid(AsA)loss,and delayed the decline in the firmness and titratable acidity(TA),and the increase in total soluble solid(TSS)in kiwifruit.Furthermore,1-MCP treatment effectively inhibited the increases in contents of acetaldehyde,ethanol,and GABA along with the suppressed activities of key enzymes involved in ethanol fermentation and GABA shunt pathway,such as pyruvate decarboxylase(PDC),alcohol dehydrogenase(ADH),glutamate decarboxylase(GAD),and GABA-transaminase(GABA-T)in kiwifruit during storage.In conclusion,1-MCP treatment efficiently regulated the ethanol fermentation and GABA shunt pathway by delaying the ripening process to avoid the alcohol off-flavor development,thereby contributed to maintaining the quality of the kiwifruit.
基金financially supported by the National Natural Science Foundation of China (31471157 and 31700266)
文摘Kiwifruit is an important fruit crop that is highly sensitive to environmental stresses,such as drought,heat,cold,water logging and phytopathogens.Therefore it is indispensable to identify stress-responsive candidate genes in kiwifruit cultivars for the stress resistance improvement.Here we report the isolation and characterization of a novel kiwifruit R1R2R3-MYB homolog(AcMYB3R)whose expression was induced by drought,salinity and cold stress.In vitro assays showed that AcMYB3R is a nuclear protein with transcriptional activation activity by binding to the cis-element of the kiwifruit orthologue of G2/M phase-specific gene KNOLLE.The Arabidopsis transgenic plants overexpressing AcMYB3R showed drastically enhanced tolerance to drought and salt stress.The expressions of stress-responsive genes such as RD29A,RD29B,COR15A and RD22 were prominently up-regulated by ectopic expression of Ac MYB3R.Our study provides a valuable piece of information for functional genomics studies of kiwifruit and molecular breeding in improving stress tolerance for crop production.
文摘以秦美猕猴桃（Actinidia deliciosa C.F．Liang et A．R．Ferguson．Vat．Qinmei）果实为材料，研究了1-甲基环丙烯（1-MCP）处理在低温下对猕猴桃果实生理和品质的影响。0～2℃冷库贮存时，1-MCP能延缓猕猴桃硬度降低、重量损失、果实中维生素C含量的下降，抑制猕猴桃果实的可溶性固形物含量上升，保持较高的好果率，有显著的保鲜效果。低温下1-MCP明显抑制猕猴桃果实的呼吸作用，降低呼吸强度和推迟呼吸高峰出现，同时延缓乙烯释放高峰出现的时间。1-MCP保鲜的机理可能存在对乙烯受体抑制和乙烯合成抑制两种机制。
