[Objective] This study aimed to investigate the prevalence and variation of porcine kobuvirus （PKV） in suckling piglets in China. [Method] In 2013-2014, 224 feces samples from suckling piglets with diarrhea in 27 pi...[Objective] This study aimed to investigate the prevalence and variation of porcine kobuvirus （PKV） in suckling piglets in China. [Method] In 2013-2014, 224 feces samples from suckling piglets with diarrhea in 27 pig farms of five provinces in China were collected to detect 3D genes of PKV with RT-PCR method; the sequences and genetic variation of 29 PKV 3D genes were analyzed. [Result] Total positive rate of PKV in feces samples from suckling piglets with diarrhea was 65.18% （146/224）; total positive rate of PKV in pig farms was 85,2% （23/27）; nucleotide sequences and the deduced amino acid sequences of 29 PKV 3D genes shared 87.0%-100% and 92.7%-100% homologies with six PKV-related 3D sequences, respectively. [Conclusion] PKV infection is prevalent in suckling piglets in China; PKV 3D genes exhibit high diversity.展开更多
Although norovirus,rotavirus,adenovirus and Astrovirus are considered the most important viral agents transmitted by food and water,in recent years other viruses,such as Aichi virus(AiV),have emerged as responsible fo...Although norovirus,rotavirus,adenovirus and Astrovirus are considered the most important viral agents transmitted by food and water,in recent years other viruses,such as Aichi virus(AiV),have emerged as responsible for gastroenteritis outbreaks associated with different foods.AiV belongs to the genus Kobuvirus of the family Picornaviridae.It is a virus with icosahedral morphology that presents a single stranded RNA genome with positive sense(8280 nucleotides)and a poly(A)chain.AiV was first detected from clinical samples and in recent years has been involved in acute gastroenteritis outbreaks from different world regions.Furthermore,several studies conducted in Japan,Germany,France,Tunisia and Spain showed a high prevalence of AiV antibodies in adults(between 80%and 99%),which is indicative of a large exposure to this virus.The aim of this review is to bring together all the discovered information about the emerging pathogen human Aichi virus(AiV),discussing the possibles routes of transmission,new detection techniques and future research.Although AiV is responsible for a low percentage of gastroenteritis outbreaks,the high seroprevalence shown by human populations indicates an evident role as an enteric agent.The low percentage of AiV detection could be explained by the fact that the pathogen is more associated to subclinical infections.Further studies will be needed to clarify the real impact of AiV in human health and its importance as a causative gastroenteritis agent worldwide.展开更多
文摘[Objective] This study aimed to investigate the prevalence and variation of porcine kobuvirus （PKV） in suckling piglets in China. [Method] In 2013-2014, 224 feces samples from suckling piglets with diarrhea in 27 pig farms of five provinces in China were collected to detect 3D genes of PKV with RT-PCR method; the sequences and genetic variation of 29 PKV 3D genes were analyzed. [Result] Total positive rate of PKV in feces samples from suckling piglets with diarrhea was 65.18% （146/224）; total positive rate of PKV in pig farms was 85,2% （23/27）; nucleotide sequences and the deduced amino acid sequences of 29 PKV 3D genes shared 87.0%-100% and 92.7%-100% homologies with six PKV-related 3D sequences, respectively. [Conclusion] PKV infection is prevalent in suckling piglets in China; PKV 3D genes exhibit high diversity.
基金support of Consellería de Educación,Universidade e Formación Profesional,Xunta de Galicia,Spain(Grant 2014-PG110),that funded the research that generated part of the results presented here.
文摘Although norovirus,rotavirus,adenovirus and Astrovirus are considered the most important viral agents transmitted by food and water,in recent years other viruses,such as Aichi virus(AiV),have emerged as responsible for gastroenteritis outbreaks associated with different foods.AiV belongs to the genus Kobuvirus of the family Picornaviridae.It is a virus with icosahedral morphology that presents a single stranded RNA genome with positive sense(8280 nucleotides)and a poly(A)chain.AiV was first detected from clinical samples and in recent years has been involved in acute gastroenteritis outbreaks from different world regions.Furthermore,several studies conducted in Japan,Germany,France,Tunisia and Spain showed a high prevalence of AiV antibodies in adults(between 80%and 99%),which is indicative of a large exposure to this virus.The aim of this review is to bring together all the discovered information about the emerging pathogen human Aichi virus(AiV),discussing the possibles routes of transmission,new detection techniques and future research.Although AiV is responsible for a low percentage of gastroenteritis outbreaks,the high seroprevalence shown by human populations indicates an evident role as an enteric agent.The low percentage of AiV detection could be explained by the fact that the pathogen is more associated to subclinical infections.Further studies will be needed to clarify the real impact of AiV in human health and its importance as a causative gastroenteritis agent worldwide.
