[Objective] Antagonistic actinomyces of Amorphophallus konjac soft rot were isolated from the soil,the antibacterial activity of the fermentation was detected,and its active components were separated.[Method] The anta...[Objective] Antagonistic actinomyces of Amorphophallus konjac soft rot were isolated from the soil,the antibacterial activity of the fermentation was detected,and its active components were separated.[Method] The antagonistic actinomycetes were screened using plate confrontation culture method.The SJK18 antagonistic strain with strong activity was conducted single-factor tests using different carbon sources,nitrogen sources,temperatures and pH value as the culture condition,and the antibacterial activities of fermentation liquid was determined,respectively.The fermentation liquid of antagonistic strain was extracted with alcohol and acetic ester,and their antibacterial activities were determined.[Result] A total of 112 strains of actinomycetes were obtained,and 11 strains had antibacterial activity.The antibacterial activity of SJK18 strain was the strongest in the single-factor test,when the corn powder was adopted as the carbon sources,KNO3 was the nitrogen source,the temperature was 30 ℃ and the pH was 6.0,the appropriate pH value for the activity was 5.5-10.0.The diameters of inhibition zone of alcohol extract and raffinate of ethyl acetate were 17.0 and 20.0 mm,respectively,while the extraction of ethyl acetate had no antibacterial activity.[Conclusion] A strain of actinomyces with strong antibacterial activity against soft rot of A.konjac was obtained.展开更多
为了开发适合吞咽困难患者食用的植物蛋白3D打印食品,本文将亚麻籽胶和魔芋胶按照质量比2:3进行复配形成复合多糖,并与豌豆分离蛋白(Pea protein isolate,PPI)进行剪切处理,探究了不同浓度的复合多糖(0.5%、0.7%、0.9%、1.1%和1.3%)对...为了开发适合吞咽困难患者食用的植物蛋白3D打印食品,本文将亚麻籽胶和魔芋胶按照质量比2:3进行复配形成复合多糖,并与豌豆分离蛋白(Pea protein isolate,PPI)进行剪切处理,探究了不同浓度的复合多糖(0.5%、0.7%、0.9%、1.1%和1.3%)对乳液凝胶粒径、流变学特性和3D打印性能的影响。并通过国际吞咽障碍标准化倡议(IDDSI)对乳液凝胶进行评估。结果表明:不同浓度的复合多糖改善乳液凝胶的效果不同。粒径测试表明随着复合多糖浓度的增加,PPI乳液凝胶的粒径呈现减小的趋势。流变学特性表明多糖的加入使PPI乳液凝胶具有粘弹性,且所有的乳液凝胶样品均显示出G'>G''。当复合多糖浓度为1.1%时,乳液凝胶的粒径最小,G'和G''值最高,粘弹性最好,打印的产品具有最清晰的纹理、最稳定的结构和较好的印刷适应性。IDDSI测试表明添加1.1%和1.3%浓度复合多糖的乳液凝胶可归类为Ⅳ级过渡性食品。综上所述,1.1%浓度的复合多糖对豌豆分离蛋白乳液凝胶3D打印性能的改善最显著,这为开发植物基3D打印油墨提供理论依据。展开更多
[Objective]The research aimed to study the total DNA extraction methods of Amorphophallas.konjac.[Method]To investigate the optimum DNA extraction method,CTAB,SDS and new modified methods for isolating genomic DNA fro...[Objective]The research aimed to study the total DNA extraction methods of Amorphophallas.konjac.[Method]To investigate the optimum DNA extraction method,CTAB,SDS and new modified methods for isolating genomic DNA from Amorphophallas.konjac leaf were studied and the isolated DNAs were examined by ultraviolet absorption test,agarose gel electrophoresis and ISSR-PCR test for comparative analysis.[Result]The results showed that the new modified method was suitable for DNA isolation from Amorphophallas.konjac plants with high purity and yield;quality of DNA could meet ISSR-PCR requirement even extracted once by the new modified method.[Conclusion]The study provided basis for extracting high quality DNA sample,and offered the molecular genetics reference for exploring the genetic diversity of Amorphophallas.konjac.展开更多
[Objective] The effects of different application amounts of nitrogen, phos-phate and potassium fertilizers on soft rot of konjac were investigated in this study. [Method] The grey correlation analysis was adopted to e...[Objective] The effects of different application amounts of nitrogen, phos-phate and potassium fertilizers on soft rot of konjac were investigated in this study. [Method] The grey correlation analysis was adopted to evaluate the correlation be-tween the application amount of nitrogen, phosphate and potassium fertilizers and the occurrence of soft rot of konjac. [Result] The excessive application of nitrogen fertilizer would induce the occurrence of soft rot of konjac, but the application of potassium fertilizer had a good control effect on soft rot. [Conclusion] The applica-tion amount of nitrogen fertilizer should be control ed reasonably in the planting of konjac, but the application amount of phosphate and potassium fertilizer could be in-creased to some extent, reducing the occurrence of soft rot of konjac.展开更多
In this paper, the hydrogen bonding network models of konjac glucomannan （KGM） are predicted in the approach of molecular dynamics （MD）. These models have been proved by experiments whose results are consistent wi...In this paper, the hydrogen bonding network models of konjac glucomannan （KGM） are predicted in the approach of molecular dynamics （MD）. These models have been proved by experiments whose results are consistent with those from simulation. The results show that the hydrogen bonding network structures of KGM are stable and the key linking points of hydrogen bonding network are at the O（6） and O（2） positions on KGM ring. Moreover, acety has significant influence on hydrogen bonding network and hydrogen bonding network structures are more stable after deacetylation.展开更多
Konjac glucomannan （KGM） was crosslinked with sodium tripolyphosphate （STPP） to synthesize hydrogels. The crosslinking reaction was confirmed by FT-IR. The results of degradation test show that the hydrogels retai...Konjac glucomannan （KGM） was crosslinked with sodium tripolyphosphate （STPP） to synthesize hydrogels. The crosslinking reaction was confirmed by FT-IR. The results of degradation test show that the hydrogels retain the enzymatic degradation character of KGM and can be degraded for 74.45% in 5 days by cellulase E0240.展开更多
The long-range structure of konjac glucomannan(KGM)is studied by using laser light scatter(LLS), gel permeation chromatography(GPC)and method of viscosidity. The weight-average molecular weight(Mw), root-mean-square r...The long-range structure of konjac glucomannan(KGM)is studied by using laser light scatter(LLS), gel permeation chromatography(GPC)and method of viscosidity. The weight-average molecular weight(Mw), root-mean-square ratio of gyration[(S2)1/2], second viral coefficient(A2)and multi-dispersion coefficient(Mw/Mn)are 1.04×106, 105. 0±0. 9 nm,(-1. 59±0.28)×10-3 mol ml g-2 and 1.015±0.003, respectively. Mark-Houwink equation is established as [η] = 5. 96×10-2Mw0.73 and the molecular chain parameters are as follows: ML=982. 82 nm-1, Lp = 27. 93 nm, d = 0. 74 nm, h = 0. 26 nm, L = l 054.11 nm. Further more molecular chain morphology of KGM is studied by using atom force microscope(AFM)and transmission electronic microscope(TEM), and the result shows that the KGM molecular is an extending semi-flexible linear chain without branch. Therefore, the image of molecular chain morphology confirms the deduction drawn by Mark-Houwink equation and molecular chain parameters.展开更多
The transformation of molecular conformation after gelatinization of konjac glucomannan is characterized by means of GC, GPC, FT-IR, DSC, XRD and TEM. The results showed that the molecule of KGM had no branch, the mon...The transformation of molecular conformation after gelatinization of konjac glucomannan is characterized by means of GC, GPC, FT-IR, DSC, XRD and TEM. The results showed that the molecule of KGM had no branch, the monose composition and connecting type of the molecular chain had no change, the transformation of molecule weight was little, and its drying powder sample formed an evidently new crystalline region. The hollow double helix structure of KGM in hydrosol changed from stretch chain into intercross and entwisted after gelatinization. The gelatin formation mechanism of konjac glucomannan is thereby expounded.展开更多
Molecular dynamics （MD） method is adopted to simulate the conformation variations of konjac glucomannan （KGM） saline solution at different temperatures, and structurally analyze the trends and reasons of viscosity...Molecular dynamics （MD） method is adopted to simulate the conformation variations of konjac glucomannan （KGM） saline solution at different temperatures, and structurally analyze the trends and reasons of viscosity change in KGM saline solution with temperature. The experimental results have been analyzed to find out that the sum of formative hydrogen bonds decreases with the rise of temperature and the amount of intramolecular hydrogen bonds suddenly increases at 323 K. Besides, in terms of molecular orbital data obtained from simulation, we can know that hydrogen bonding energy also decreases with the rise of temperature. Therefore, we can predict the viscosity of KGM saline solution decreases gradually when rising the temperature.展开更多
The simulation is carried out by employing the method of molecular dynamics with the single chain of konjac glucomannan （KGM） in vacuum as the structural model to discuss the factors that affect the single chain str...The simulation is carried out by employing the method of molecular dynamics with the single chain of konjac glucomannan （KGM） in vacuum as the structural model to discuss the factors that affect the single chain structure, the dynamic structure of the chain and the acting forces that maintain the chain structure. The results show that the shape and stability of the chain are affected by the degree of polymerization. As for the KGM with high degree of polymerization, its chain presents random coiling state and its stability declines. Both before and after deacetylation in the process of dynamic motion, the chain of KGM presents random coiling state with periodic variation of extension and coil and demonstrates favorable flexibility, indicating acetyl is not the main factor that affects the shape of chain, whereas dihedral angle and static actions are respectively the key bonding and nonbonding acting forces that influence the single chain conformations in vacuum.展开更多
Biological activity of konjac glucomannan is closely related to its structure,in particular to its high-level structure.Researches on the activity mechanism of konjac glucomannan are significant for revealing the myst...Biological activity of konjac glucomannan is closely related to its structure,in particular to its high-level structure.Researches on the activity mechanism of konjac glucomannan are significant for revealing the mysteries of participation in life activity.In this paper,analysis of the effects of konjac glucomannan configuration and various factors on its structure and activity was conducted,mechanism of biological activity of konjac glucomannan was explored,and the hot research topic of konjac glucomannan was given.展开更多
In this work, the formation sites, helical parameters and hydrogen bond positions of Konjac glucomannan molecular helices were investigated using molecular dynamic simulation method. To our interest, the KGM chain is ...In this work, the formation sites, helical parameters and hydrogen bond positions of Konjac glucomannan molecular helices were investigated using molecular dynamic simulation method. To our interest, the KGM chain is mainly composed by local left and right helix struetttres. The formation sites of KGM chain might locate at the chain-segments containing acetyl groups, and the left helix is the favorable conformation of KGM. Temperature-dependent molecule conformation study indicates that the right helix is dominant when the temperature is lower than 343 K, above which, however, the left helix is dominating （right helix disappears）. In addition, intramolecular hydrogen bonds in the left helix can be found at the -OH groups on C（2）, C（4） and C（6） of mannose residues; comparably, the intramolecular hydrogen bonds in the right helix can be mainly observed at the -OH groups on C（4） and C（6） of the mannose residues and C（3） of the glucose residues. In conclusion, molecular dynamic simulation is an efficient method for the microscopic conformation study of glucomannan molecular helices.展开更多
Based on the knot theory and researching of network structures of glucomannan molecules, the polysaccharides were analyzed. The link prediction analysis is to further reveal the interactions between polysaccharides, t...Based on the knot theory and researching of network structures of glucomannan molecules, the polysaccharides were analyzed. The link prediction analysis is to further reveal the interactions between polysaccharides, to elaborate QSAR of polysaccharides, and to analyze the network conformation relationships among polysaccharides. We made a classification for glucomannan molecules based on the related domestic and international theories, and investigated their network structures and application prospects. The knot theory and the link predictions not only simplify the glucomannan microscopic descriptions but also play a guiding role in predicting and regulating the structures.展开更多
Konjac glucomannan （KGM） was aminated by 2-chloroethyl-amine （CEA） as reagent so as to study the influence of concentration of CEA （based on the amount of KGM）, concentration of NaOH, reaction time and temperatu...Konjac glucomannan （KGM） was aminated by 2-chloroethyl-amine （CEA） as reagent so as to study the influence of concentration of CEA （based on the amount of KGM）, concentration of NaOH, reaction time and temperature on the extent of amination. And the molecular simulation technology was adopted to analyze the conformation stability of aminate （AKGM）. The results indicate that when the amount of CEA is higher, the extent of amination is higher. The optimum concentration of NaOH, reaction time and temperature are 10% NaOH, 70 ℃ and 45 rain, respectively. IR shows KGM is successfully aminated. The conformation of AKGM is in a random clew-like shape.展开更多
To understand the effect of plasma treatment on the Konjac glucomannan film, the nitrogen plasma was injected into the film by ion beam injection machine in this study. The structures and properties of Konjac glucoman...To understand the effect of plasma treatment on the Konjac glucomannan film, the nitrogen plasma was injected into the film by ion beam injection machine in this study. The structures and properties of Konjac glucomannan film after plasma treatment were analyzed by Infrared spectroscopy, Raman spectrum, X-ray, ect. The result showed that nitrogen groups appeared in the KGM molecular chain and part of this chain fractured, and the number of hydrogen bonds increased after the treatment of plasma. The form of KGM molecule remained amorphous non-crystalline state, but the crystalline region was increased and became more ordered. The mechanical property of tensile strength and breaking elongation was improved, while the WVP was decreased. The nitrogen groups were grafted on the KGM molecular chain after plasma treatment, which led to the improvement of the properties of KGM film.展开更多
基金Supported by Key Laboratory Fund in Hubei Province(2008NP11)Science and Technology Research and Development Project in Yichang City(A09302-17)~~
文摘[Objective] Antagonistic actinomyces of Amorphophallus konjac soft rot were isolated from the soil,the antibacterial activity of the fermentation was detected,and its active components were separated.[Method] The antagonistic actinomycetes were screened using plate confrontation culture method.The SJK18 antagonistic strain with strong activity was conducted single-factor tests using different carbon sources,nitrogen sources,temperatures and pH value as the culture condition,and the antibacterial activities of fermentation liquid was determined,respectively.The fermentation liquid of antagonistic strain was extracted with alcohol and acetic ester,and their antibacterial activities were determined.[Result] A total of 112 strains of actinomycetes were obtained,and 11 strains had antibacterial activity.The antibacterial activity of SJK18 strain was the strongest in the single-factor test,when the corn powder was adopted as the carbon sources,KNO3 was the nitrogen source,the temperature was 30 ℃ and the pH was 6.0,the appropriate pH value for the activity was 5.5-10.0.The diameters of inhibition zone of alcohol extract and raffinate of ethyl acetate were 17.0 and 20.0 mm,respectively,while the extraction of ethyl acetate had no antibacterial activity.[Conclusion] A strain of actinomyces with strong antibacterial activity against soft rot of A.konjac was obtained.
文摘为了开发适合吞咽困难患者食用的植物蛋白3D打印食品,本文将亚麻籽胶和魔芋胶按照质量比2:3进行复配形成复合多糖,并与豌豆分离蛋白(Pea protein isolate,PPI)进行剪切处理,探究了不同浓度的复合多糖(0.5%、0.7%、0.9%、1.1%和1.3%)对乳液凝胶粒径、流变学特性和3D打印性能的影响。并通过国际吞咽障碍标准化倡议(IDDSI)对乳液凝胶进行评估。结果表明:不同浓度的复合多糖改善乳液凝胶的效果不同。粒径测试表明随着复合多糖浓度的增加,PPI乳液凝胶的粒径呈现减小的趋势。流变学特性表明多糖的加入使PPI乳液凝胶具有粘弹性,且所有的乳液凝胶样品均显示出G'>G''。当复合多糖浓度为1.1%时,乳液凝胶的粒径最小,G'和G''值最高,粘弹性最好,打印的产品具有最清晰的纹理、最稳定的结构和较好的印刷适应性。IDDSI测试表明添加1.1%和1.3%浓度复合多糖的乳液凝胶可归类为Ⅳ级过渡性食品。综上所述,1.1%浓度的复合多糖对豌豆分离蛋白乳液凝胶3D打印性能的改善最显著,这为开发植物基3D打印油墨提供理论依据。
基金Supported by Specialized Research Fund from Shaanxi Institute of Technology (SLGQD0716)~~
文摘[Objective]The research aimed to study the total DNA extraction methods of Amorphophallas.konjac.[Method]To investigate the optimum DNA extraction method,CTAB,SDS and new modified methods for isolating genomic DNA from Amorphophallas.konjac leaf were studied and the isolated DNAs were examined by ultraviolet absorption test,agarose gel electrophoresis and ISSR-PCR test for comparative analysis.[Result]The results showed that the new modified method was suitable for DNA isolation from Amorphophallas.konjac plants with high purity and yield;quality of DNA could meet ISSR-PCR requirement even extracted once by the new modified method.[Conclusion]The study provided basis for extracting high quality DNA sample,and offered the molecular genetics reference for exploring the genetic diversity of Amorphophallas.konjac.
基金Supported by Scientific Research Fund of Yunnan Provincial Department of Education(2012C1402012Z024)~~
文摘[Objective] The effects of different application amounts of nitrogen, phos-phate and potassium fertilizers on soft rot of konjac were investigated in this study. [Method] The grey correlation analysis was adopted to evaluate the correlation be-tween the application amount of nitrogen, phosphate and potassium fertilizers and the occurrence of soft rot of konjac. [Result] The excessive application of nitrogen fertilizer would induce the occurrence of soft rot of konjac, but the application of potassium fertilizer had a good control effect on soft rot. [Conclusion] The applica-tion amount of nitrogen fertilizer should be control ed reasonably in the planting of konjac, but the application amount of phosphate and potassium fertilizer could be in-creased to some extent, reducing the occurrence of soft rot of konjac.
基金supported by the National Natural Science Foundation of China(30371009, 30471218) Science Foundation of Fujian Department of Education (JA03059)
文摘In this paper, the hydrogen bonding network models of konjac glucomannan （KGM） are predicted in the approach of molecular dynamics （MD）. These models have been proved by experiments whose results are consistent with those from simulation. The results show that the hydrogen bonding network structures of KGM are stable and the key linking points of hydrogen bonding network are at the O（6） and O（2） positions on KGM ring. Moreover, acety has significant influence on hydrogen bonding network and hydrogen bonding network structures are more stable after deacetylation.
基金The authors are grateful for the financial support of the National Science Foundation of China(Grant No.20174029)National Key Basic Research and Development Program(G1999064703).
文摘Konjac glucomannan （KGM） was crosslinked with sodium tripolyphosphate （STPP） to synthesize hydrogels. The crosslinking reaction was confirmed by FT-IR. The results of degradation test show that the hydrogels retain the enzymatic degradation character of KGM and can be degraded for 74.45% in 5 days by cellulase E0240.
文摘The long-range structure of konjac glucomannan(KGM)is studied by using laser light scatter(LLS), gel permeation chromatography(GPC)and method of viscosidity. The weight-average molecular weight(Mw), root-mean-square ratio of gyration[(S2)1/2], second viral coefficient(A2)and multi-dispersion coefficient(Mw/Mn)are 1.04×106, 105. 0±0. 9 nm,(-1. 59±0.28)×10-3 mol ml g-2 and 1.015±0.003, respectively. Mark-Houwink equation is established as [η] = 5. 96×10-2Mw0.73 and the molecular chain parameters are as follows: ML=982. 82 nm-1, Lp = 27. 93 nm, d = 0. 74 nm, h = 0. 26 nm, L = l 054.11 nm. Further more molecular chain morphology of KGM is studied by using atom force microscope(AFM)and transmission electronic microscope(TEM), and the result shows that the KGM molecular is an extending semi-flexible linear chain without branch. Therefore, the image of molecular chain morphology confirms the deduction drawn by Mark-Houwink equation and molecular chain parameters.
文摘The transformation of molecular conformation after gelatinization of konjac glucomannan is characterized by means of GC, GPC, FT-IR, DSC, XRD and TEM. The results showed that the molecule of KGM had no branch, the monose composition and connecting type of the molecular chain had no change, the transformation of molecule weight was little, and its drying powder sample formed an evidently new crystalline region. The hollow double helix structure of KGM in hydrosol changed from stretch chain into intercross and entwisted after gelatinization. The gelatin formation mechanism of konjac glucomannan is thereby expounded.
基金Supported by the National Natural Science Foundation of China (30371009)Science Foundation of Fujian Department of Education (JA03059)Key Project of Science and Technology of Fujian Province (2003Y008)
文摘Molecular dynamics （MD） method is adopted to simulate the conformation variations of konjac glucomannan （KGM） saline solution at different temperatures, and structurally analyze the trends and reasons of viscosity change in KGM saline solution with temperature. The experimental results have been analyzed to find out that the sum of formative hydrogen bonds decreases with the rise of temperature and the amount of intramolecular hydrogen bonds suddenly increases at 323 K. Besides, in terms of molecular orbital data obtained from simulation, we can know that hydrogen bonding energy also decreases with the rise of temperature. Therefore, we can predict the viscosity of KGM saline solution decreases gradually when rising the temperature.
基金This work was supported by the National Natural Science Foundation of China (30371009), Science Foundation of Fujian Department of Education (JA03059) and Key Project of Science and Technology of Fujian Province (2003Y008)
文摘The simulation is carried out by employing the method of molecular dynamics with the single chain of konjac glucomannan （KGM） in vacuum as the structural model to discuss the factors that affect the single chain structure, the dynamic structure of the chain and the acting forces that maintain the chain structure. The results show that the shape and stability of the chain are affected by the degree of polymerization. As for the KGM with high degree of polymerization, its chain presents random coiling state and its stability declines. Both before and after deacetylation in the process of dynamic motion, the chain of KGM presents random coiling state with periodic variation of extension and coil and demonstrates favorable flexibility, indicating acetyl is not the main factor that affects the shape of chain, whereas dihedral angle and static actions are respectively the key bonding and nonbonding acting forces that influence the single chain conformations in vacuum.
基金Sponsored by the National Natural Science Foundation of China (No.30871749,30901004 and 31071518)Special Research Fund of Institution of Higher Learning of Ministry of Education for the Doctoral Program of Joint Funding(20113515110010)+3 种基金Natural Science Foundation of Fujian Province (No.2011J01285)a scientific problem-tackling project of Guangdong Province (No.2010B080701079)a Guangzhou Yangcheng Scholar Scientific Project (No.10B005D)Fuzhou Science and Technology Plan Project (2011-N-44)
文摘Biological activity of konjac glucomannan is closely related to its structure,in particular to its high-level structure.Researches on the activity mechanism of konjac glucomannan are significant for revealing the mysteries of participation in life activity.In this paper,analysis of the effects of konjac glucomannan configuration and various factors on its structure and activity was conducted,mechanism of biological activity of konjac glucomannan was explored,and the hot research topic of konjac glucomannan was given.
基金supported by the National Natural Science Foundation of China (30871749,30901004)Natural Science Foundation of Fujian Province(2009J01061)
文摘In this work, the formation sites, helical parameters and hydrogen bond positions of Konjac glucomannan molecular helices were investigated using molecular dynamic simulation method. To our interest, the KGM chain is mainly composed by local left and right helix struetttres. The formation sites of KGM chain might locate at the chain-segments containing acetyl groups, and the left helix is the favorable conformation of KGM. Temperature-dependent molecule conformation study indicates that the right helix is dominant when the temperature is lower than 343 K, above which, however, the left helix is dominating （right helix disappears）. In addition, intramolecular hydrogen bonds in the left helix can be found at the -OH groups on C（2）, C（4） and C（6） of mannose residues; comparably, the intramolecular hydrogen bonds in the right helix can be mainly observed at the -OH groups on C（4） and C（6） of the mannose residues and C（3） of the glucose residues. In conclusion, molecular dynamic simulation is an efficient method for the microscopic conformation study of glucomannan molecular helices.
基金Supported by the National Natural Science Foundation of China(31271837 and 31071518)Specialized Research Fund for the Doctoral Program of Higher Education jointly funded by Ministry of Education(20113515110010)+2 种基金Special Research Funds from Ministry of Science and Technology(2012GA7200022)Major projects of industries,universities and research in Fujian Province(2013N5003)Natural Science Foundation of Fujian Province(2011J0101)
文摘Based on the knot theory and researching of network structures of glucomannan molecules, the polysaccharides were analyzed. The link prediction analysis is to further reveal the interactions between polysaccharides, to elaborate QSAR of polysaccharides, and to analyze the network conformation relationships among polysaccharides. We made a classification for glucomannan molecules based on the related domestic and international theories, and investigated their network structures and application prospects. The knot theory and the link predictions not only simplify the glucomannan microscopic descriptions but also play a guiding role in predicting and regulating the structures.
基金Supported by Foundation of Minnan Normal University,the Funding(type A,No.JA11167)from the Fujian Education DepartmentNational Natural Science Foundation of China(31071518 and 31271837)+2 种基金Joint Specialized Research Fund for the Doctoral Program of Higher Education,MOE(20113515110010)Science and Technology Planning Project of technological department(2012GA7200022)Natural Science Foundation of Fujian Province(2011J01285)
文摘Konjac glucomannan （KGM） was aminated by 2-chloroethyl-amine （CEA） as reagent so as to study the influence of concentration of CEA （based on the amount of KGM）, concentration of NaOH, reaction time and temperature on the extent of amination. And the molecular simulation technology was adopted to analyze the conformation stability of aminate （AKGM）. The results indicate that when the amount of CEA is higher, the extent of amination is higher. The optimum concentration of NaOH, reaction time and temperature are 10% NaOH, 70 ℃ and 45 rain, respectively. IR shows KGM is successfully aminated. The conformation of AKGM is in a random clew-like shape.
基金sponsored by the National Natural Science Foundation of China(No.30901004,31071518 and 31271837)the Fuzhou Science and Technology Plan(No.2011-N-44)+1 种基金the Guangdong Province Science and Technology Plan(No.2010B080701079)Guangzhou Yangcheng Scholar Scientific Project in Universities(No.10B005D)
文摘To understand the effect of plasma treatment on the Konjac glucomannan film, the nitrogen plasma was injected into the film by ion beam injection machine in this study. The structures and properties of Konjac glucomannan film after plasma treatment were analyzed by Infrared spectroscopy, Raman spectrum, X-ray, ect. The result showed that nitrogen groups appeared in the KGM molecular chain and part of this chain fractured, and the number of hydrogen bonds increased after the treatment of plasma. The form of KGM molecule remained amorphous non-crystalline state, but the crystalline region was increased and became more ordered. The mechanical property of tensile strength and breaking elongation was improved, while the WVP was decreased. The nitrogen groups were grafted on the KGM molecular chain after plasma treatment, which led to the improvement of the properties of KGM film.
