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Microscopic Characteristics, Chromatographic Profiles and Inhibition of Peroxidase Activity of the Leaves of <i>Manihot esculenta</i>and <i>Manihot glaziovii</i>, Consumed as Traditional Vegetables
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作者 Paulin Mutwale Kapepula Patricia Mbombo Mungitshi +7 位作者 Dieudonné Tshitenge Tshitenge Thierry Franck Dieudonné Mumba Ngoyi Pascal Dibungi T. Kalenda Monique Tits Michel Frédérich Nadege Kabamba Ngombe Ange Mouithys-Mickalad 《Journal of Biosciences and Medicines》 2021年第9期59-73,共15页
Methanolic extracts from the leaves of <em>Manihot esculenta </em>(Two cultivars) and <em>Manihot glaziovii</em>, consumed as traditional vegetables in DR. Congo was chemically characterized by... Methanolic extracts from the leaves of <em>Manihot esculenta </em>(Two cultivars) and <em>Manihot glaziovii</em>, consumed as traditional vegetables in DR. Congo was chemically characterized by Thin layer Chromatography and High Performance Liquid Chromatography. <em>In vitro</em> biochemical activities of extracts against Radical Oxidative Species (ROS) production were assessed in cellular models, on enzymes, Myeloperoxidase (MPO) and Horseradish Peroxidase (HRP) involved in inflammation. The microscopic analysis of the powder of leaves showed that each species displays specific and discriminating botanical microscopic features. Varieties of<em> M. esculenta</em> had a chemical fingerprint different from <em>M. glaziovii</em>. The majority of compounds were polyphenols, represented mainly by rutin, kaempferol-3-O-rutinoside, amentoflavone, phenolic acids such as gallic acid. All extracts exhibited high cellular antioxidant activity in the range of 0.1 to 10 μg<span style="white-space:nowrap;">·</span>mL<sup>-1</sup> using lucigenin with neutrophils, but a moderate cellular antioxidant activity ranging between 10 and 100 μg<span style="white-space:nowrap;">·</span>mL<sup>-1</sup> with DCFDA on HL60 monocytes. Extracts from <em>Manihot</em> leaves showed a pronounced inhibitory effect on the production of extracellular ROS, on HRP and myeloperoxidase activity. Cellular antioxidant activities, the inhibitory effect on HRP of extracts from <em>M. glaziovii</em>, <em>M. esculenta</em> cultivar <em>Mwambu </em>were significantly higher, but their inhibitory effect on the activity of MPO was lower than those of <em>M. esculenta</em> cultivar TEM 419. The biological activities of <em>Manihot esculenta</em> and <em>Manihot glaziovii </em>were well correlated to their phytochemicals that could justify their traditional use as vegetables, potential functional foods or nutraceutical resources and medicines. 展开更多
关键词 Horseradish Peroxidase Kahemba konzo Manihot esculenta Manihot glaziovii MYELOPEROXIDASE Traditional Vegetable
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代谢营养性和中毒性脊髓病
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作者 刘琳 蒋雨平 张云云 《中国临床神经科学》 2013年第5期554-556,595,共4页
在整理近10年余国内外文献基础上,就维生素B12、叶酸、铜、维生素E缺乏,山黧豆中毒等引起的代谢营养性和中毒性脊髓病,从病因、发病机制、临床表现、诊断和治疗方面进行综述。
关键词 脊髓病 维生素B12 维生素E 叶酸 铜缺乏 山黧豆 中毒 konzo
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